期刊
CARBOHYDRATE POLYMERS
卷 110, 期 -, 页码 142-147出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2014.03.060
关键词
Lonicera japonica Thunb.; A beta(42); Glucan; Aggregation and neurotoxicity; Alzheimer's disease
资金
- New Drug Creation and Manufacturing Program [2012ZX09301001-003]
- National Science Fund [81125025]
- National Natural Science Foundation of China [31230022]
- Industry-University-Research Institute Alliance Fund in Guangdong Province, China [2010A090200041]
- Shanghai Research Program [13ZR1447500]
Inhibition of A beta aggregation and attenuation of its cytotoxicity are considered to valuable therapeutics for Alzheimer's disease (AD). Here, a glucan named as LJW0F2 was purified from flowers of Lonicera japonica Thunb. Using monosaccharides composition analysis, methylation analysis, IR and NMR spectroscopy, this polysaccharide was elucidated to be an alpha-D-(1 -> 4)-glucan with an alpha-(1 -> 4) linked branch attached to the C-6 position. Its inhibitory effect on A beta(42) aggregation was measured by fluorescence spectroscopic analysis with thioflavine T (ThT) and atomic force microscopy (AFM). We showed that polysaccharide LJW0F2 could inhibit A beta(42) aggregation in a dose-dependent-manner. Besides, LJW0F2 could attenuate the cytotoxicity induced by A beta(42) aggregation in SH-SY5Y neuroblastoma cells. To the best of our knowledge, this was the first report that the exogenous plant-derived polysaccharide might block A beta(42) aggregation directly and reduce its toxicity in SH-SY5Y cells. (C) 2014 Elsevier Ltd. All rights reserved.
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