期刊
CARBOHYDRATE POLYMERS
卷 101, 期 -, 页码 484-492出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2013.09.052
关键词
Heparin; Heparinases; Low molecular weight heparin; Enzymatic depolymerization
资金
- National High Technology Research and Development Program of China [2012AA022201E]
- National Nature Science Foundation of China [20836004]
- China Postdoctoral Science Foundation [20100470140]
Enzymatic depolymerization of heparin by heparinases is promising for production of low molecular weight heparins (LMWHs) as anticoagulants, due to its mild reaction conditions and high selectivity. Here, different heparinase combinations were used to depolymerize heparin. Heparinase I and heparinase II can depolymerize heparin more efficiently than heparinase III, respectively, but heparinase III was the best able to protect the anticoagulant activities of LMWHs. Heparinase III and heparinase I/II combinations were able to efficiently depolymerize heparin to LMWHs with higher anticoagulant activity than the LMWHs produced by the respective heparinase land heparinase II. HepIII and HepI is the best combination for maintaining high anti-ha activity (75.7 +/- 4.21 IU/mg) at the same M-w value. Furthermore, considering both the changes in molecular weight and anticoagulant activity, the action patterns of heparinase I and heparinase II were found not to follow the exolytic and processive depolymerizing mechanism from the reducing end of heparin. (C) 2013 Elsevier Ltd. All rights reserved.
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