期刊
CARBOHYDRATE POLYMERS
卷 93, 期 1, 页码 57-64出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2012.05.016
关键词
Lactic acid bacteria; Homopolysaccharides; Heteropolysaccharides; Production; Purification; Methods
Lactic acid bacteria (LAB) produce homopolysaccharides (HOPS) and heteropolysaccharides (HePS) with potential functional properties. In this work, we have performed a comparative analysis of production and purification trials of these biopolymers from bacterial culture supernatants. LAB strains belonging to four different genera, both natural as well as recombinant, were used as model systems for the production of HoPS and HePS. Two well characterized strains carrying the gft gene were used for beta-glucan production, Pediococcus parvulus 2.6 (P. parvulus 2.6) isolated from cider, and the recombinant strain Lactococcus lactis NZ9000[pGTF] (L lactis NZ9000[pGTF]). In addition, another cider isolate, Lactobacillus suebicus CUPV225 (L. suebicus CUPV225), and Leuconostoc mesenteroides RTF10 (L mesenteroides RTF10), isolated from meat products were included in the study. Chemical analysis of the EPS revealed that L mesenteroides produces a dextran, L suebicus a complex heteropolysaccharide, and the beta-glucan producing-strains the expected 2-substituted (1,3)-beta-glucan. (C) 2012 Elsevier Ltd. All rights reserved.
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