Article
Medicine, General & Internal
Benjamin Lardinois, Michael Hardy, Isabelle Michaux, Geoffrey Horlait, Thomas Rotens, Hugues Jacqmin, Sarah Lessire, Pierre Bulpa, Alain Dive, Francois Mullier
Summary: This study compared a point-of-care version of APTT with laboratory-based APTT and measurements of anti-Xa activity in terms of correlation, agreement, and turnaround time (TAT). The results showed that although the use of the POCT-APTT device significantly reduced the time to results, the results obtained were poorly consistent with those obtained by lab-APTT or anti-Xa activity and should not be used with the nomograms developed for lab-APTT.
JOURNAL OF CLINICAL MEDICINE
(2022)
Article
Hematology
Dominique Lasne, Marie Toussaint-Hacquard, Celine Delassasseigne, Anne Bauters, Claire Flaujac, Philippe Savard, Christine Mouton, Emmanuel De Maistre, Alain Stepanian, Valerie Eschwege, Maxime Delrue, Jean-Louis Georges, Antoine Gros, Alexandre Mansour, Guillaume Leroy, Romain Jouffroy, Matthieu Mattei, Antoine Beurton, Adeline Pontis, Marie Neuwirth, Fabienne Nedelec-Gac, Thomas Lecompte, Emmanuel Curis, Virginie Siguret, Isabelle Gouin-Thibault
Summary: This study aimed to evaluate the impact of different reagents and blood collection tubes on unfractionated heparin (UFH) anti-Xa levels. The results showed that reagents containing Dextran Sulfate (DS) significantly increased the anti-Xa levels, while blood samples collected with CTAD tubes had slightly higher anti-Xa levels compared to citrate. These differences may have clinical implications in treatment decisions.
THROMBOSIS AND HAEMOSTASIS
(2023)
Article
Medicine, General & Internal
Anna Matyjek, Aleksandra Rymarz, Zuzanna Nowicka, Slawomir Literacki, Tomasz Rozmyslowicz, Stanislaw Niemczyk
Summary: The study found that the efficacy of enoxaparin is reduced in severe nephrotic syndrome patients, and the dose should be adjusted based on ideal body weight to achieve target anti-FXa activity.
JOURNAL OF CLINICAL MEDICINE
(2021)
Article
Hematology
Pierre Toulon, Motalib Smahi, Neila De Pooter
Summary: The study aimed to compare the anti-Xa-correlated APTT therapeutic ranges calculated using different combinations of APTT and anti-Xa reagents in frozen citrated plasmas from 87 inpatients on UFH. Results showed that the APTT therapeutic ranges calculated based on different combinations of reagents varied significantly in correlation with anti-Xa activities between 0.30 and 0.70 IU/ml.
JOURNAL OF THROMBOSIS AND HAEMOSTASIS
(2021)
Article
Medicine, General & Internal
Nada Riahi, Laurence Rozen, Anne Demulder
Summary: This study aims to evaluate if a heparin-calibrated anti-Xa assay could be suitable to estimate the concentrations of apixaban and rivaroxaban and establish an algorithm for clinicians in emergency situations. The results show that this method can accurately predict or exclude the presence of DOACs when specific tests are not available.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Medicine, General & Internal
Tuukka Helin, Tomi Tirri, Heidi Korkala, Kimmo Lappalainen, Lotta Joutsi-Korhonen
Summary: The study aimed to validate the use of activated clotting time (ACT) in monitoring unfractionated heparin (UFH) in endovascular radiology. The results showed a moderate correlation between ACT and anti-Xa levels, indicating that ACT monitoring of UFH in endovascular radiology is feasible.
Article
Hematology
Michael Hardy, Julien Cabo, Antoine Deliege, Jonathan Douxfils, Isabelle Gouin-Thibault, Thomas Lecompte, Francois Mullier
Summary: This study investigated the impact of dextran sulfate (DS) on anti-Xa assays for unfractionated heparin (UFH) monitoring. The results showed that DS increased the anti-Xa effect, leading to higher FXa inhibition than predicted. In the presence of heparin antagonists, DS also increased anti-Xa levels, further affecting the accuracy of the assay results.
RESEARCH AND PRACTICE IN THROMBOSIS AND HAEMOSTASIS
(2023)
Article
Hematology
Guido Willekens, Jan-Dirk Studt, Adriana Mendez, Lorenzo Alberio, Pierre Fontana, Walter A. Wuillemin, Adrian Schmidt, Lukas Graf, Bernhard Gerber, Cedric Bovet, Thomas C. Sauter, Michael Nagler
Summary: The newly developed universal anti-Xa assay simplifies drug concentration measurement in laboratory procedures and can be widely implemented. Through a prospective study in clinical practice, this method has been shown to accurately measure the concentrations of rivaroxaban, apixaban, and edoxaban, and predict relevant clinical levels effectively.
BRITISH JOURNAL OF HAEMATOLOGY
(2021)
Article
Surgery
Julie Elizabeth Farrar, Molly Elizabeth Droege, Carolyn Dosen Philpott, Eric William Mueller, Neil Edward Ernst, Amy Teres Makley, Kaley Marie Deichstetter, Christopher Allen Droege
Summary: This study found that trauma patients with higher subprophylactic anti-Xa concentrations had higher total body weight and lower weight-adjusted enoxaparin dose. However, these factors did not result in differences in the incidence of VTE or red blood cell transfusions.
JOURNAL OF SURGICAL RESEARCH
(2021)
Article
Hematology
Shravan Morla, Hiroshi Deguchi, John H. Griffin
Summary: The study found that skeletal muscle myosin and cardiac myosin can neutralize the enhancement of antithrombin's anticoagulant activity by unfractionated heparin, but the length of the heparin chain can have different effects on this interaction.
JOURNAL OF THROMBOSIS AND HAEMOSTASIS
(2021)
Review
Hematology
Amalie Baun Kjaergaard, Jens Fuglsang, Anne-Mette Hvas
Summary: Prophylactic dosing strategies with weight dosed, fixed dosed, or anti-Xa adjusted LMWH showed similar effectiveness and safety in pregnant women. Therapeutic weight-adjusted LMWH and weight plus anti-Xa-adjusted LMWH provided comparable results in effectiveness and safety for pregnant women with VTE or high thromboembolic risk. Pregnant women with mechanical heart valves receiving therapeutic anti-Xa-adjusted LMWH had mixed outcomes, with some experiencing thrombosis and bleeding within target anti-Xa ranges. According to the systematic review, there is currently no evidence to support anti-Xa monitoring when using LMWH during pregnancy, but further investigation may be needed for pregnant women with MHV.
SEMINARS IN THROMBOSIS AND HEMOSTASIS
(2021)
Article
Clinical Neurology
Charlyne Brakta, Alain Stepanian, Peggy Reiner, Maxime Delrue, Mikael Mazighi, Emmanuel Curis, Virginie Siguret
Summary: This study developed and validated an easy-to-use nomogram that accurately predicts DOAC concentrations based on LMWH-anti-Xa results in emergency situations. Using DOAC <50 ng/mL equivalent threshold, instead of the fixed LMWH <0.5 IU/mL one, would allow proposing thrombolysis to more patients.
Article
Pediatrics
Alba Perez Ortiz, Carl E. Dempfle, Toni Jung, Thalia Doniga, Christel Weiss, Svetlana Hetjens, Thomas Schaible, Neysan Rafat
Summary: The study found that anti-factor Xa levels correlate better with heparin dose than activated clotting time in newborns with congenital diaphragmatic hernia on ECMO support. Using anti-factor Xa assay instead of activated clotting time for dosing of unfractionated heparin could reduce thrombotic complications in this population. Thromboelastometry showed no additional benefit for this purpose.
FRONTIERS IN PEDIATRICS
(2021)
Article
Clinical Neurology
Casey C. May, Santino Cua, Keaton S. Smetana, Ciaran J. Powers
Summary: In this study, male, heavier weight, or morbidly obese neurosurgical patients were less likely to have anti-Xa levels within the target range. Higher anti-Xa levels may predispose patients to major bleeding, as shown in previous research.
WORLD NEUROSURGERY
(2022)
Article
Surgery
Rachel Bigos, Edmond Solomon, Jon D. Dorfman, Michael Ha
Summary: The study evaluated a weight-based and anti-Xa-guided enoxaparin dosing protocol in ICU trauma patients. The protocol was safe and effective in achieving initial anti-Xa levels within goal, but monitoring may be beneficial for patients over 100 kg to prevent sub-prophylactic levels.
JOURNAL OF SURGICAL RESEARCH
(2021)
Article
Chemistry, Medicinal
Hugo Groult, Remi Cousin, Caroline Chot-Plassot, Maheva Maura, Nicolas Bridiau, Jean-Marie Piot, Thierry Maugard, Ingrid Fruitier-Arnaudin
Article
Oncology
Yahya Ashraf, Hanane Mansouri, Valerie Laurent-Matha, Lindsay B. Alcaraz, Pascal Roger, Severine Guiu, Danielle Derocq, Gautier Robin, Henri-Alexandre Michaud, Helene Delpech, Marta Jarlier, Martine Pugniere, Bruno Robert, Anthony Puel, Lucie Martin, Flavie Landomiel, Thomas Bourquard, Oussama Achour, Ingrid Fruitier-Arnaudin, Alexandre Pichard, Emmanuel Deshayes, Andrei Turtoi, Anne Poupon, Joelle Simony-Lafontaine, Florence Boissiere-Michot, Nelly Pirot, Florence Bernex, William Jacot, Stanislas du Manoir, Charles Theillet, Jean-Pierre Pouget, Isabelle Navarro-Teulon, Nathalie Bonnefoy, Andre Pelegrin, Thierry Chardes, Pierre Martineau, Emmanuelle Liaudet-Coopman
JOURNAL FOR IMMUNOTHERAPY OF CANCER
(2019)
Article
Chemistry, Medicinal
Amandine Adrien, Antoine Bonnet, Delphine Dufour, Stanislas Baudouin, Thierry Maugard, Nicolas Bridiau
Article
Biochemistry & Molecular Biology
Justine Bodin, Amandine Adrien, Pierre-Edouard Bodet, Delphine Dufour, Stanislas Baudouin, Thierry Maugard, Nicolas Bridiau
Review
Chemistry, Medicinal
Heitor Gomes de Araujo-Filho, Jucilene F. dos Santos, Mikaella T. B. Carvalho, Laurent Picot, Ingrid Fruitier-Arnaudin, Hugo Groult, Lucindo J. Quintans-Junior, Jullyana S. S. Quintans
Summary: Limonene (LIM), a monoterpene abundant in Citrus fruits peels, has shown potential as a natural anticancer compound by inhibiting tumor initiation, growth, and angiogenesis, as well as inducing cancer cells apoptosis.
PHYTOTHERAPY RESEARCH
(2021)
Article
Nutrition & Dietetics
Marine Dupuit, Vivien Chavanelle, Benoit Chassaing, Fanny Perriere, Monique Etienne, Claire Plissonneau, Audrey Boscaro, Nicolas Barnich, Vincent Pialoux, Thierry Maugard, Florian Le Joubioux, Sebastien Peltier, Pascal Sirvent, Yolanda F. Otero, Nathalie Boisseau
Summary: The combination of high-intensity interval training (HIIT) and TOTUM-63 has shown to limit weight gain, improve glycemic control, increase colon mucosa microbiota diversity, and enhance the relative abundance of beneficial bacteria. These results suggest that this combination could be effective for managing obesity and prediabetes.
Article
Microbiology
Camille Maziere, Helene Agogue, Cristiana Cravo-Laureau, Christine Cagnon, Isabelle Lanneluc, Sophie Sable, Ingrid Fruitier-Arnaudin, Christine Dupuy, Robert Duran
Summary: The study revealed that microbial mats from exploited and abandoned salterns exhibit different bacterial and meiofaunal diversity, with higher salinity in exploited sites leading to lower bacterial diversity but higher eukaryotic diversity. The presence of certain bacterial and eukaryotic groups in the mats may be attributed to human actions such as salt recovery practices in exploited salterns.
MICROBIOLOGICAL RESEARCH
(2021)
Article
Chemistry, Medicinal
Jennifer Saliba, Chanez Manseur, Hugo Groult, Hussein Akil, Mona Tannoury, Danielle Troutaud, Thierry Maugard, Jean Feuillard, Ingrid Arnaudin, Chantal Jayat-Vignoles
Summary: EBV infection is widespread and can lead to lymphomas. Both LCLs and DLBCLs cells overexpressing PD-L1 can inhibit T-cell response. Fucoidan, extracted from brown seaweed, shows potential for reducing PD-L1 expression and improving treatment of lymphoma cells. This finding has implications for immunotherapy.
Article
Chemistry, Medicinal
Chanez Manseur, Hugo Groult, Manon Porta, Pierre-Edouard Bodet, Rachida Mersni-Achour, Raphaelle Petit, Samir Ali-Moussa, Benjamin Musnier, Didier Le Cerf, Tony Varacavoudin, Oualid Haddad, Angela Sutton, Cintia Emi Yanaguibashi Leal, Edilson Beserra Alencar-Filho, Jean-Marie Piot, Nicolas Bridiau, Thierry Maugard, Ingrid Fruitier-Arnaudin
Summary: Oligosaccharides derived from ?-carrageenan (?-COs) have shown promise in regulating heparanase activity and have potential therapeutic applications in cancer treatment. Commercial ?-carrageenan is a mixture of different CAR families and its composition does not reflect the real composition, limiting its clinical use. By comparing six commercial ?-CARs and applying H2O2-assisted depolymerization, ?-CO formulations with similar molar masses and sulfation degrees to those suitable for antitumor properties were obtained. However, the anti-HPSE activity of these new ?-COs was affected by other features, such as differences in the initial mixture composition and degradation induced by H2O2-based hydrolysis.
Article
Chemistry, Multidisciplinary
Hugo Groult, Susana Carregal-Romero, David Castejon, Mikel Azkargorta, Ana-Beatriz Miguel-Coello, Krishna Reddy Pulagam, Vanessa Gomez-Vallejo, Remi Cousin, Maria Munoz-Caffarel, Charles H. Lawrie, Jordi Llop, Jean-Marie Piot, Felix Elortza, Thierry Maugard, Jesus Ruiz-Cabello, Ingrid Fruitier-Arnaudin
Summary: This study successfully prepared HEP-ESIONP probes with excellent MRI contrast using a one-pot microwave method with heparin oligosaccharides (HO) of different lengths as coatings, demonstrating anticoagulant properties and/or specific antitumor activity based on the length of HO used. In vivo distribution study showed significant changes in probe behaviors and renal clearance with shortening of HO length, while in vitro characterization of protein coronas provided insight into the crucial impact of HO length on opsonization-mediated immune response and elimination. The precise HO length identified in this study resulted in ESIONP probes with prolonged vascular lifetime, moderate tumor accumulation, low uptake by non-specific organs, and favorable urinary clearance, meeting prerequisites for advanced theranostic medical applications with dual MRI/PET hot spot capability and potential antitumor activity.
Review
Chemistry, Applied
Xingran Kou, Dongdong Su, Fei Pan, Xiwei Xu, Qingran Meng, Qinfei Ke
Summary: This review provides a systematic discussion of the application of molecular dynamics (MD) simulations in aroma compounds (ACs)/cyclodextrins (CDs) inclusion complexes (ICs). It covers the establishment of the simulation process, parameter selection, model evaluation, and various application cases, summarizing the major achievements and challenges of this method.
CARBOHYDRATE POLYMERS
(2024)
Review
Chemistry, Applied
Haoyu Yu, Quanbin Zhang, Ammad Ahmad Farooqi, Jing Wang, Yang Yue, Lihua Geng, Ning Wu
Summary: Brown algae are rich in fucoidan, which has been found to have anti-cancer and anti-metastasis effects. Fucoidan inhibits tumor cell growth, proliferation, and metastasis, and also promotes immune responses in the tumor microenvironment.
CARBOHYDRATE POLYMERS
(2024)
Review
Chemistry, Applied
Liang Dong, Yanan Li, Hailin Cong, Bing Yu, Youqing Shen
Summary: Gene therapy is a revolutionary treatment that requires suitable vectors for protecting and releasing exogenous nucleic acids in target cells. Chitosan, as a non-viral vector, has gained attention due to its good biocompatibility and ability to load large amounts of nucleic acids. This paper summarizes the potential of chitosan and its derivatives as gene delivery vector materials, discusses factors influencing transfection efficiency, performance evaluation, ways to optimize infectious efficiency, and current research development directions. It also provides an outlook on the future prospects of chitosan.
CARBOHYDRATE POLYMERS
(2024)
Article
Chemistry, Applied
Ana Iglesias-Mejuto, Beatriz Magarinos, Tania Ferreira-Goncalves, Ricardo Starbird-Perez, Carmen Alvarez-Lorenzo, Catarina Pinto Reis, Ines Ardao, Carlos A. Garcia-Gonzalez
Summary: This study developed a novel processing strategy to manufacture drug-loaded and personalized aerogels with nanostructures. The aerogels demonstrated bioactivity and antimicrobial effects, promoting bone regeneration and preventing infections in bone tissue engineering.
CARBOHYDRATE POLYMERS
(2024)
Article
Chemistry, Applied
Zhenxu Shi, Dimei Yang, Yan Zhou, Xinyu Chen, Lin Gan, Jin Huang
Summary: This study proposes a micro-assembly method to improve the photoluminescent properties of crystalline cellulose nanocrystals (CNCs) by organizing them within a sub-micrometer-sized metal-organic framework and coating with TiO2. The TiO2 coating prevents CNC assembly breakdown and allows information to be revealed using screenprinted labels for anti-counterfeiting purposes.
CARBOHYDRATE POLYMERS
(2024)
Article
Chemistry, Applied
Xuejiao Liu, Bin Fu, Jierong Chen, Zhenyu Sun, Dongdong Zheng, Zhonghua Li, Bing Gu, Ying Zhang, Haojie Lu
Summary: Liver disease is a major cause of global mortality, and identifying biomarkers for diagnosing its progression is crucial for improving outcomes. Targeted mass spectrometry is a powerful tool for verifying biomarker candidates and clinical applications, particularly for glycoproteins translation. However, the limitation of analyzing only one sample per run has become apparent. In this study, a high-throughput intact N-glycopeptides quantification strategy was developed, allowing the validation of 20 samples per run with an average analysis time of 3 minutes per sample. The strategy was applied in a cohort of 461 serum samples and identified a panel of 10 IgG N-glycopeptides that have strong clinical utility in evaluating the severity of liver disease.
CARBOHYDRATE POLYMERS
(2024)
Article
Chemistry, Applied
Qian Wang, Hao Jiang, Hongli Zhang, Weiqiao Lu, Xiao Wang, Wenfeng Xu, Jia Li, Youjing Lv, Guoyun Li, Chao Cai, Guangli Yu
Summary: This study proposes a novel strategy of antibody-beta-glucan conjugates (AGC) to enhance the antitumor immune response to immune checkpoint blockade (ICB) therapy. AGC demonstrated powerful tumor suppression and promoted interaction between tumor cells and dendritic cells (DCs), thereby enhancing immunotherapeutic benefits.
CARBOHYDRATE POLYMERS
(2024)
Article
Chemistry, Applied
Chaeyeon Lee, Sojeong Lee, Sang Wook Kang
Summary: The study aims to enhance the properties of porous membranes by addressing the limitations associated with phase separation. By using cellulose acetate and citric acid, the researchers were able to fabricate membranes with improved mechanical strength and thermal stability. The cross-linking effect of citric acid resulted in a more uniform pore structure and higher porosity.
CARBOHYDRATE POLYMERS
(2024)
Review
Chemistry, Applied
Tejaswini Kolipaka, Giriraj Pandey, Noella Abraham, Dadi A. Srinivasarao, Rajeev Singh Raghuvanshi, P. S. Rajinikanth, Vidya Tickoo, Saurabh Srivastava
Summary: This review focuses on the design and application of polysaccharide-based hydrogel wound dressings, highlighting aspects such as biocompatibility, biodegradability, drug entrapment, moisturizing ability, swelling, and mechanical properties. Additionally, various crosslinking methods and recent developments in stimuli-responsive hydrogels are discussed.
CARBOHYDRATE POLYMERS
(2024)
Article
Chemistry, Applied
Anqi Xu, Nan Zhang, Shixing Su, Hongyu Shi, Daoqiang Lu, Xifeng Li, Xin Zhang, Xin Feng, Zhuohua Wen, Gengwu Ma, Mengshi Huang, Chi Huang, Yuqi Hu, Hao Yuan, Qinwen Liu, Daogang Guan, Jun Wang, Chuanzhi Duan
Summary: The study presents a one-pot radical polymerization method to fabricate a hydrogel with adhesive properties, which can effectively treat bacterial-infected diabetic wounds and accelerate wound healing.
CARBOHYDRATE POLYMERS
(2024)
Article
Chemistry, Applied
Montarop Yamabhai, Munthipha Khamphio, Thae Thae Min, Chai Noy Soem, Nguyen Cao Cuong, Waheni Rizki Aprilia, Krisanai Luesukprasert, Karsidete Teeranitayatarn, Atthaphon Maneedaeng, Tina R. Tuveng, Silje B. Lorentzen, Simen Antonsen, Paiboon Jitprasertwong, Vincent G. H. Eijsink
Summary: The study investigates the bioconversion of chitosan into soluble anti-inflammatory chitosan oligosaccharides (CHOS) using an enzyme. The results show that the generated CHOS have anti-inflammatory activity, but the magnitude of the activity depends on the substrate and production process. Different methods of dissolving chitosan also affect the properties of CHOS. The study highlights the importance of quality assurance in CHOS preparations.
CARBOHYDRATE POLYMERS
(2024)
Article
Chemistry, Applied
Wen Cao, Jiangping Wu, Xinya Zhao, Zixu Li, Jie Yu, Taili Shao, Xuefeng Hou, Lutan Zhou, Chunfei Wang, Guodong Wang, Jun Han
Summary: In this study, a water-soluble polysaccharide (PLP1) was successfully isolated and purified from Pueraria lobata. It was found that PLP1 was composed of specific glycosidic units and exhibited a better free radical-scavenging ability. Moreover, PLP1 effectively protected the liver against acute alcoholic liver disease (ALD) in mice.
CARBOHYDRATE POLYMERS
(2024)
Article
Chemistry, Applied
Ya Su, Yaqian Liu, Xueyan Hu, Yueqi Lu, Jinyuan Zhang, Wenbo Jin, Wang Liu, Yan Shu, Yuen Yee Cheng, Wenfang Li, Yi Nie, Bo Pan, Kedong Song
Summary: A 3D printed scaffold based on carbon dots-curcumin nano-drug release has been developed for drug delivery after breast cancer surgery. The scaffold showed effective inhibition of tumor growth, antibacterial activity, and promotion of wound healing, making it a promising approach for preventing tumor recurrence.
CARBOHYDRATE POLYMERS
(2024)
Article
Chemistry, Applied
Ekaterina Sokolova, Diane Jouanneau, Antonin Chevenier, Murielle Jam, Nathalie Desban, Pierre Colas, Elizabeth Ficko-Blean, Gurvan Michel
Summary: Carrageenans, a compound synthesized in red algae, have various biological properties and are valuable in the pharmaceutical and cosmetic industries. Their fine structure affects wound healing, oxidative processes, hemostasis, and inflammation. Enzymatic modification of carrageenans produces oligosaccharides that bind to natural human serum antibodies and specific antibodies, showing potential for therapeutic applications.
CARBOHYDRATE POLYMERS
(2024)
Article
Chemistry, Applied
Yan Shi, Miao Yu, Kaijin Qiu, Tiantian Kong, Chunjing Guo, Wenxue Zhang, Daquan Chen, Ming Kong
Summary: In this study, functionalized transfersomes were developed to co-deliver doxorubicin and 1MT towards primary tumors and tumor draining lymph nodes via transdermal administration using microneedles. The results showed that the nano-complexed microneedles exhibited a stronger suppression in tumor growth compared to the intravenous group.
CARBOHYDRATE POLYMERS
(2024)