期刊
CARBOHYDRATE POLYMERS
卷 84, 期 3, 页码 997-1003出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2010.12.058
关键词
Pholiota dinghuensis Bi; Polysaccharide; Production; Preliminary characterization; Antitumor activity
资金
- National Natural Science Foundation of China [30870547]
The production, preliminary characterization and antitumor activity in vitro of mycelial polysaccharides from Pholiota dinghuensis Bi (PDP) were investigated in the present study. Firstly, crude PDP was prepared from the mycelia of P. dinghuensis Bi by submerged culture. Then, the crude PDP was purified by chromatography of DEAE-52 cellulose and Sephadex G-100, resulting three purified fractions of PDP-1. PDP-2 and PDP-3. We found that the monosaccharide composition of PDP-3 was greatly different from that of PDP-1 or PDP-2. In addition, it contained the highest contents of protein, sulfate and uronic acid among the polysaccharides tested. Furthermore, PDP-3 exhibited higher antiproliferative activity against human gastric cancer BGC-823 cells in vitro than crude PDP. PDP-1 or PDP-2. At a concentration of 400 mg/L and an exposure time of 72 h, the inhibition rates for crude PDP, PDP-2 and PDP-3 were 69.36%, 73.65% and 85.78%, respectively. (C) 2010 Elsevier Ltd. All rights reserved.
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