期刊
CARBOHYDRATE POLYMERS
卷 83, 期 2, 页码 554-560出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2010.08.024
关键词
Gynostemma pentaphyllum Makino; Sulfated derivatives; Ionic liquid; Catalyst; Antitumor activity
资金
- National Natural Science Foundation of China (NSFC) [20775029]
- Program for New Century Excellent Talents in University [NCET-07-0400]
- Lanzhou University
A native polysaccharides (GPP2) was isolated from Gynostemma pentaphyllum Makino Four sulfated derivatives of GPP2 (GPP2-s1 to s4) were prepared by chlorosulfonic acid method with ionic liquids (ILs) as solvent and 4-dimethylaminopyridine (DMAP) as catalyst The structures of the sulfated derivatives were analyzed by FT-IR spectra and C-13 NMR spectra which indicated that the sulfated groups were introduced mainly at the C-6 position as well as at the C-2 position The products showed different degree of substitution (DS) ranging from 0 79 to 1 34 and different weight-average molecular mass (Mw) ranging from 8 64 to 11 2 Da The sulfated derivatives inhibited the growth of HepG2 cells and Hela cells in vitro significantly Furthermore they had no obvious influence on 293 cells which indicated that they had low toxicity Flow cytometric studies revealed that treatment of the sulfated derivatives with Hela cells could mediate the cell-cycle arrest in the S phase (C) 2010 Elsevier Ltd All rights reserved
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