期刊
CARBOHYDRATE POLYMERS
卷 84, 期 2, 页码 743-750出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2010.06.023
关键词
A. coerulea; C-13 labeled chitosan; Fungal mycelia; G. butleri; Solid substrate fermentation; Submerged fermentation
资金
- Grants-in-Aid for Scientific Research [22500417] Funding Source: KAKEN
Nowadays, chitin and chitosan are applied in many medical and pharmaceutical products. However little is known about the metabolism of chitin and chitosan in vivo. In the human body, lysozyme will degrade chitin and chitosan into chito-oligosaccharides. C-13 labeled chitosan is an essential prerequisite for the further study of the fate of chito-oligosaccharides in vivo. To fulfill this requirement, chitosans were extracted from mycelia of fungi. Absidia coerulea ATCC 14076 and Gongronella butleri USDB 0201 and ATCC 42618 grown in solid substrate fermentation (SSF) and submerged fermentation (SMF) to select the best fungus and fermentation method for the production of C-13 labeled chitosan. Based on the production yield of chitosan, the SSF method is the best method for the production of fungal chitosan when compared with SMF methods (i.e., fed-batch fermentation and batch fermentation). However synthesis of C-13 labeled chitosan in cell wall of G. butleri grown in SSF medium coated with 1-C-13-glucose was not observed. Alternatively, fungus A. coerulea was grown in SMF medium containing 2-C-13-glucose. The successful synthesis of C-13 labeled glucosamine from 2-C-13-glucose was observed in mycelia of A. coerulea grown in SMF medium containing 2-C-13-glucose in a yield of about 13 g/100 g mycelia. (C) 2010 Elsevier Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据