期刊
CARBOHYDRATE POLYMERS
卷 79, 期 4, 页码 811-817出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2009.08.028
关键词
Ginseng pectin; Rhamnogalacturonan I; Structural analysis; Arabinogalactan
资金
- National Natural Science Foundation of China [30770489, 30973857]
- Natural Science Foundation of Jilin Province [20070710]
- Hongjiu Ginseng Co. Ltd
- Analysis and Testing Foundation of Northeast Normal University
Five rhamnogalacturonan I (RG-I) domains RG-I-1, RG-I-2, RG-I-3A, RG-I-3B and RC-I-4 were isolated from ginseng pectin by endo-polygalacturonase hydrolysis and a combination of ion-exchange and gel-permeation chromatography. The five domains all contain galacturonic acid, rhamnose, galactose and arabinose as main components and their rhamnose/galacturonic acid is from 0.26 to 0.64, among the range of RG-I The molecular weights of RG-I-1 (5 kDa), RG-I-2 (4 kDa) and RG-I-3B (6 kDa) are smaller than those of RG-I-3A (45 kDa) and RG-I-4 (60 kDa). C-13 NMR spectra of all domains showed RG-I features. RG-I-2 and RG-I-3B contained RG-I domains linked with highly methyl-esterified and acetylated homogalacturonan domains and the side chains probably belonging to type I and type II arabinogalactans, while RG-I-3A and RG-I-4 may have the side chains of type I arabinogalactans. 4-0-methyl-beta-D-glucuronic acid residues were present at non-reducing terminals of RG-I-2. (C) 2009 Elsevier Ltd. All rights reserved.
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