Article
Biochemistry & Molecular Biology
Laura Nicolle, Jens Casper, Melanie Willimann, Celine M. A. Journot, Pascal Detampel, Tomaz Einfalt, Hiu Man Grisch-Chan, Beat Thony, Sandrine Gerber-Lemaire, Jorg Huwyler
Summary: The study developed a novel chitosan-derivative, dCS-Suc-LPEI-14, showing promising biocompatibility, cytotoxicity, and transfection efficiency. Through in vitro and in vivo experiments, its potential for non-viral gene therapy was confirmed.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Jens Casper, Laura Nicolle, Melanie Willimann, Evrim Umit Kuzucu, Alan Tran, Perrine Robin, Pascal Detampel, Hiu Man Grisch-Chan, Beat Thony, Jorg Huwyler, Sandrine Gerber-Lemaire
Summary: The study investigates the improvement of nucleic acid delivery by using depolymerized chitosan-polyethylenimine DNA complexes. This modular approach combines core-dCS-linear PEI/DNA complexes with a shell consisting of dCS-PEG-COOH, resulting in improved nucleic acid encapsulation, cellular uptake, and transfection potency. The optimized ternary complex shows high reporter gene signal for three days after administration, indicating its potential for in vitro and in vivo gene delivery.
MACROMOLECULAR BIOSCIENCE
(2023)
Article
Chemistry, Multidisciplinary
Han Qin, Yujie Ji, Guangyue Li, Xiaohui Xu, Chuangwei Zhang, Wenjie Zhong, Shihan Xu, Yuanyuan Yin, Jinlin Song
Summary: This study developed a nanocomplex based on graphene oxide (GO) for effective cellular delivery of miR-29b, which plays a crucial role in bone formation. The nanocomplex, functionalized with polyethyleneglycol (PEG) and polyethylenimine (PEI), showed good biocompatibility, miRNA loading capacity, and transfection efficiency. The encapsulation of miR-29b/GO-PEG-PEI nanocomplex into chitosan (CS) hydrogel successfully promoted osteogenesis in vitro and in vivo.
FRONTIERS IN CHEMISTRY
(2022)
Article
Chemistry, Applied
Jun Wang, Justin Liu, Dao-Qiang Lu, Lijing Chen, Rujia Yang, Dahai Liu, Bin Zhang
Summary: In this study, diselenide-crosslinked nanoparticles were successfully fabricated for doxorubicin delivery. The nanoparticles exhibited desirable morphology and size distribution, and the starting material used improved biocompatibility and physiological stability. The high drug encapsulation efficiency of the nanoparticles was achieved through electrostatic interactions between doxorubicin and the material. In vitro and in vivo experiments demonstrated that the doxorubicin-loaded diselenide-crosslinked nanoparticles had higher drug accumulation and enhanced growth inhibition compared to the control samples.
CARBOHYDRATE POLYMERS
(2022)
Review
Chemistry, Applied
Zohreh Mohammadi, Maryam Eini, Ali Rastegari, Morteza Rafiee Tehrani
Summary: Numerous studies have demonstrated the significant role of biomolecules in treating diseases, but the challenge lies in designing efficient delivery systems. Chitosan, a unique biopolymer with special properties, has been widely utilized for biomolecule delivery, with research groups focusing on its versatile application.
CARBOHYDRATE POLYMERS
(2021)
Article
Biochemistry & Molecular Biology
Reena Singh, Pradeep Kumar
Summary: PEIs have been modified with disaccharides to produce organic nanoparticles for efficient gene delivery, showing improved cell viability and transfection efficiency. Among them, trehalose-PEI nanoparticles exhibit higher pDNA transportation potential and anticancer activity.
BIOORGANIC CHEMISTRY
(2021)
Article
Chemistry, Physical
Fereshteh Hassani, Amir Heydarinasab, Homayon Ahmad Panahi, Elham Moniri
Summary: In this study, a pH and thermosensitive polymer-modified polyethylenimine dendrimer-grafted tungsten disulfide nanocarrier was successfully synthesized for triggered letrozole delivery in cancer chemotherapy. The nanocarrier exhibited dual responsiveness, with drug release enhanced under near infrared laser irradiation. The pH and thermosensitivity of the nanocarrier were demonstrated through release experiments at different pH values and temperatures. The release of letrozole from the nanocarrier followed non-Fickian diffusion mechanism, and the adsorption of the drug on the pH and thermosensitive polymer was best explained by Langmuir isotherm and pseudo-second-order kinetic models.
JOURNAL OF MOLECULAR LIQUIDS
(2023)
Article
Biotechnology & Applied Microbiology
Ramzi Mukred Saeed, Mohammed Abdullah, Mamoun Ahram, Mutasem Omar Taha
Summary: The study developed novel ellipsoid non-cytotoxic NPs crosslinked with TPP, utilizing the advantages of CSP and LC to efficiently deliver siRNA into cancer cells. The NPs showed excellent stability and cellular penetration, achieving significant silencing efficiency against SLUG protein.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Engineering, Environmental
Wenting Zheng, Ratnakar Reddy Kuchukulla, Xiangning Xu, Dandan Zhang, Lihong Zhou, Qingle Zeng
Summary: The article introduces a novel adsorbing material CC-GP-D with comb-shaped structure, prepared with chitosan as the main chain, which shows higher adsorption efficiency and cost-effectiveness in removing heavy metal ions from sewage.
JOURNAL OF POLYMERS AND THE ENVIRONMENT
(2022)
Article
Biotechnology & Applied Microbiology
Jae Been Lee, Yun Hwan Park, Min Seo Jeon, Sok Kim, Yoon-E Choi
Summary: In this study, the researchers successfully increased the production of astaxanthin in Haematococcus pluvialis by combining polyethylenimine (PEI) with chitosan. The polyethylenimine concentration had an effect on astaxanthin accumulation, while the molecular weight of chitosan did not. The treatment also increased the reactive oxygen species content in cells, leading to the upregulation of enzymes involved in astaxanthin biosynthesis. The PEI-chitosan fiber treatment showed high recyclability and maintained over 90% astaxanthin production efficiency.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Biochemical Research Methods
Raghu Ramanathan, Nathan A. Delvaux, Kevin G. Rice
Summary: We developed an improved in vitro transfection assay for testing the efficiency of non-viral vector DNA nanoparticle transfection of primary hepatocytes. The assay was optimized for cell plating number, DNA dose, and PEI to DNA ratio, and provides reliable in vitro expression results for direct comparison of different non-viral gene delivery vectors.
ANALYTICAL BIOCHEMISTRY
(2022)
Review
Chemistry, Applied
Liang Dong, Yanan Li, Hailin Cong, Bing Yu, Youqing Shen
Summary: Gene therapy is a revolutionary treatment that requires suitable vectors for protecting and releasing exogenous nucleic acids in target cells. Chitosan, as a non-viral vector, has gained attention due to its good biocompatibility and ability to load large amounts of nucleic acids. This paper summarizes the potential of chitosan and its derivatives as gene delivery vector materials, discusses factors influencing transfection efficiency, performance evaluation, ways to optimize infectious efficiency, and current research development directions. It also provides an outlook on the future prospects of chitosan.
CARBOHYDRATE POLYMERS
(2024)
Article
Chemistry, Multidisciplinary
David Arango, Javier Cifuentes, Paola Ruiz Puentes, Tatiana Beltran, Amaury Bittar, Camila Ocasion, Carolina Munoz-Camargo, Natasha I. Bloch, Luis H. Reyes, Juan C. Cruz
Summary: Gene delivery using nanostructured vehicles, such as iron oxide nanoparticles, shows promise for gene therapy due to their versatility, biocompatibility, and strong magnetization. In this study, an iron oxide nanoparticle-based delivery vehicle was developed to release nucleic acids under reducing conditions. The developed nanocarriers demonstrated excellent biocompatibility and efficient cargo penetration and release. This represents the first magnetic nanoparticle-based nanocarrier capable of delivering nucleic sequences while preserving functionality under specific reducing conditions.
Article
Biochemistry & Molecular Biology
Tony Z. Jia, Niraja Bapat, Ajay Verma, Irena Mamajanov, H. James Cleaves, Kuhan Chandru
Summary: This study demonstrates that minor modifications in polyester composition can significantly impact the functional properties of primitive compartments, suggesting a process by which primitive chemical systems can increase diversity of compartment phenotype through simple chemical modifications.
Article
Biochemistry & Molecular Biology
Hong Zhou, Fenglin Wan, Yufan Jian, Fuyou Guo, Miao Zhang, Shiyao Shi, Liang Yang, Shili Li, Ying Liu, Wei Ding
Summary: This study elucidates the molecular mechanism by which chitosan nanoparticles-based RNAi enhances the efficiency of dsRNA delivery. Chitosan/dsRNA polyplex nanoparticles (PNs) were found to upregulate the clathrin heavy chain (CHC) gene, thereby activating the clathrin-dependent endocytosis (CDE) pathway. The PNs directly bound to the CHC protein, a core component in CDE, to advance RNAi efficiency.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Review
Chemistry, Applied
Xingran Kou, Dongdong Su, Fei Pan, Xiwei Xu, Qingran Meng, Qinfei Ke
Summary: This review provides a systematic discussion of the application of molecular dynamics (MD) simulations in aroma compounds (ACs)/cyclodextrins (CDs) inclusion complexes (ICs). It covers the establishment of the simulation process, parameter selection, model evaluation, and various application cases, summarizing the major achievements and challenges of this method.
CARBOHYDRATE POLYMERS
(2024)
Review
Chemistry, Applied
Haoyu Yu, Quanbin Zhang, Ammad Ahmad Farooqi, Jing Wang, Yang Yue, Lihua Geng, Ning Wu
Summary: Brown algae are rich in fucoidan, which has been found to have anti-cancer and anti-metastasis effects. Fucoidan inhibits tumor cell growth, proliferation, and metastasis, and also promotes immune responses in the tumor microenvironment.
CARBOHYDRATE POLYMERS
(2024)
Review
Chemistry, Applied
Liang Dong, Yanan Li, Hailin Cong, Bing Yu, Youqing Shen
Summary: Gene therapy is a revolutionary treatment that requires suitable vectors for protecting and releasing exogenous nucleic acids in target cells. Chitosan, as a non-viral vector, has gained attention due to its good biocompatibility and ability to load large amounts of nucleic acids. This paper summarizes the potential of chitosan and its derivatives as gene delivery vector materials, discusses factors influencing transfection efficiency, performance evaluation, ways to optimize infectious efficiency, and current research development directions. It also provides an outlook on the future prospects of chitosan.
CARBOHYDRATE POLYMERS
(2024)
Article
Chemistry, Applied
Ana Iglesias-Mejuto, Beatriz Magarinos, Tania Ferreira-Goncalves, Ricardo Starbird-Perez, Carmen Alvarez-Lorenzo, Catarina Pinto Reis, Ines Ardao, Carlos A. Garcia-Gonzalez
Summary: This study developed a novel processing strategy to manufacture drug-loaded and personalized aerogels with nanostructures. The aerogels demonstrated bioactivity and antimicrobial effects, promoting bone regeneration and preventing infections in bone tissue engineering.
CARBOHYDRATE POLYMERS
(2024)
Article
Chemistry, Applied
Zhenxu Shi, Dimei Yang, Yan Zhou, Xinyu Chen, Lin Gan, Jin Huang
Summary: This study proposes a micro-assembly method to improve the photoluminescent properties of crystalline cellulose nanocrystals (CNCs) by organizing them within a sub-micrometer-sized metal-organic framework and coating with TiO2. The TiO2 coating prevents CNC assembly breakdown and allows information to be revealed using screenprinted labels for anti-counterfeiting purposes.
CARBOHYDRATE POLYMERS
(2024)
Article
Chemistry, Applied
Xuejiao Liu, Bin Fu, Jierong Chen, Zhenyu Sun, Dongdong Zheng, Zhonghua Li, Bing Gu, Ying Zhang, Haojie Lu
Summary: Liver disease is a major cause of global mortality, and identifying biomarkers for diagnosing its progression is crucial for improving outcomes. Targeted mass spectrometry is a powerful tool for verifying biomarker candidates and clinical applications, particularly for glycoproteins translation. However, the limitation of analyzing only one sample per run has become apparent. In this study, a high-throughput intact N-glycopeptides quantification strategy was developed, allowing the validation of 20 samples per run with an average analysis time of 3 minutes per sample. The strategy was applied in a cohort of 461 serum samples and identified a panel of 10 IgG N-glycopeptides that have strong clinical utility in evaluating the severity of liver disease.
CARBOHYDRATE POLYMERS
(2024)
Article
Chemistry, Applied
Qian Wang, Hao Jiang, Hongli Zhang, Weiqiao Lu, Xiao Wang, Wenfeng Xu, Jia Li, Youjing Lv, Guoyun Li, Chao Cai, Guangli Yu
Summary: This study proposes a novel strategy of antibody-beta-glucan conjugates (AGC) to enhance the antitumor immune response to immune checkpoint blockade (ICB) therapy. AGC demonstrated powerful tumor suppression and promoted interaction between tumor cells and dendritic cells (DCs), thereby enhancing immunotherapeutic benefits.
CARBOHYDRATE POLYMERS
(2024)
Article
Chemistry, Applied
Chaeyeon Lee, Sojeong Lee, Sang Wook Kang
Summary: The study aims to enhance the properties of porous membranes by addressing the limitations associated with phase separation. By using cellulose acetate and citric acid, the researchers were able to fabricate membranes with improved mechanical strength and thermal stability. The cross-linking effect of citric acid resulted in a more uniform pore structure and higher porosity.
CARBOHYDRATE POLYMERS
(2024)
Review
Chemistry, Applied
Tejaswini Kolipaka, Giriraj Pandey, Noella Abraham, Dadi A. Srinivasarao, Rajeev Singh Raghuvanshi, P. S. Rajinikanth, Vidya Tickoo, Saurabh Srivastava
Summary: This review focuses on the design and application of polysaccharide-based hydrogel wound dressings, highlighting aspects such as biocompatibility, biodegradability, drug entrapment, moisturizing ability, swelling, and mechanical properties. Additionally, various crosslinking methods and recent developments in stimuli-responsive hydrogels are discussed.
CARBOHYDRATE POLYMERS
(2024)
Article
Chemistry, Applied
Anqi Xu, Nan Zhang, Shixing Su, Hongyu Shi, Daoqiang Lu, Xifeng Li, Xin Zhang, Xin Feng, Zhuohua Wen, Gengwu Ma, Mengshi Huang, Chi Huang, Yuqi Hu, Hao Yuan, Qinwen Liu, Daogang Guan, Jun Wang, Chuanzhi Duan
Summary: The study presents a one-pot radical polymerization method to fabricate a hydrogel with adhesive properties, which can effectively treat bacterial-infected diabetic wounds and accelerate wound healing.
CARBOHYDRATE POLYMERS
(2024)
Article
Chemistry, Applied
Montarop Yamabhai, Munthipha Khamphio, Thae Thae Min, Chai Noy Soem, Nguyen Cao Cuong, Waheni Rizki Aprilia, Krisanai Luesukprasert, Karsidete Teeranitayatarn, Atthaphon Maneedaeng, Tina R. Tuveng, Silje B. Lorentzen, Simen Antonsen, Paiboon Jitprasertwong, Vincent G. H. Eijsink
Summary: The study investigates the bioconversion of chitosan into soluble anti-inflammatory chitosan oligosaccharides (CHOS) using an enzyme. The results show that the generated CHOS have anti-inflammatory activity, but the magnitude of the activity depends on the substrate and production process. Different methods of dissolving chitosan also affect the properties of CHOS. The study highlights the importance of quality assurance in CHOS preparations.
CARBOHYDRATE POLYMERS
(2024)
Article
Chemistry, Applied
Wen Cao, Jiangping Wu, Xinya Zhao, Zixu Li, Jie Yu, Taili Shao, Xuefeng Hou, Lutan Zhou, Chunfei Wang, Guodong Wang, Jun Han
Summary: In this study, a water-soluble polysaccharide (PLP1) was successfully isolated and purified from Pueraria lobata. It was found that PLP1 was composed of specific glycosidic units and exhibited a better free radical-scavenging ability. Moreover, PLP1 effectively protected the liver against acute alcoholic liver disease (ALD) in mice.
CARBOHYDRATE POLYMERS
(2024)
Article
Chemistry, Applied
Ya Su, Yaqian Liu, Xueyan Hu, Yueqi Lu, Jinyuan Zhang, Wenbo Jin, Wang Liu, Yan Shu, Yuen Yee Cheng, Wenfang Li, Yi Nie, Bo Pan, Kedong Song
Summary: A 3D printed scaffold based on carbon dots-curcumin nano-drug release has been developed for drug delivery after breast cancer surgery. The scaffold showed effective inhibition of tumor growth, antibacterial activity, and promotion of wound healing, making it a promising approach for preventing tumor recurrence.
CARBOHYDRATE POLYMERS
(2024)
Article
Chemistry, Applied
Ekaterina Sokolova, Diane Jouanneau, Antonin Chevenier, Murielle Jam, Nathalie Desban, Pierre Colas, Elizabeth Ficko-Blean, Gurvan Michel
Summary: Carrageenans, a compound synthesized in red algae, have various biological properties and are valuable in the pharmaceutical and cosmetic industries. Their fine structure affects wound healing, oxidative processes, hemostasis, and inflammation. Enzymatic modification of carrageenans produces oligosaccharides that bind to natural human serum antibodies and specific antibodies, showing potential for therapeutic applications.
CARBOHYDRATE POLYMERS
(2024)
Article
Chemistry, Applied
Yan Shi, Miao Yu, Kaijin Qiu, Tiantian Kong, Chunjing Guo, Wenxue Zhang, Daquan Chen, Ming Kong
Summary: In this study, functionalized transfersomes were developed to co-deliver doxorubicin and 1MT towards primary tumors and tumor draining lymph nodes via transdermal administration using microneedles. The results showed that the nano-complexed microneedles exhibited a stronger suppression in tumor growth compared to the intravenous group.
CARBOHYDRATE POLYMERS
(2024)