期刊
CARBOHYDRATE POLYMERS
卷 80, 期 4, 页码 1042-1047出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.carbpol.2010.01.022
关键词
Chitosan/alginate nanoparticles; EGFR; Antisense; Cytotoxicity; RT-PCR; Immunocytochemistry
资金
- office of vice-chancellor for research of TUMS [4206]
Chitosan/alginate nanoparticles optimized for size and loading efficiency were evaluated for their potential of antisense oligonucleotide delivery. The antisense for epidermal growth factor receptor (EGFR) that is over-expressed in many cancer cells was loaded in chitosan/alginate nanoparticles. The T47D breast cancer cell line was chosen to study the efficiency of optimized nanoparticles (chitosan/alginate 1:1, alginate/calcium chloride 0.2% and N/P ratio of 5 and 25) on EGFR expression. The MU cytotoxicity evaluation of nanoparticles confirmed non-toxic properties of these carriers. The FITC-labeled EGFR antisense showed that T47D cells can uptake antisense-loaded nanoparticles better than naked antisense. Both RT-PCR and immunocytochemistry analyses showed that nanoparticles with N/P ratio of 5 can down-regulate the expression of EGFR in T47D breast cancer cell line by improving internalization and stability of antisense molecules. (C) 2010 Elsevier Ltd. All rights reserved.
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