Article
Biochemistry & Molecular Biology
Kaito Kobayashi, Hisaka Shimizu, Nobukiyo Tanaka, Kouji Kuramochi, Hiroyuki Nakai, Masahiro Nakajima, Hayao Taguchi
Summary: The IALB_1185 protein is a novel enzyme that plays a key role in glucooligosaccharide transfer reaction. It exhibits unique enzymatic activity and substrate specificity towards beta-1,2-glucooligosaccharides, which is different from known enzymes.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Multidisciplinary Sciences
Oghenesivwe Osiebe, Isaac Olusanjo Adewale, Bridget Okiemute Omafuvbe
Summary: This study reports a cost-effective method for producing industrial enzyme invertase using inexpensive materials. The results showed that using cassava-soybean medium increased the production of invertase compared to yeast sucrose broth medium. The enzyme was purified and characterized, and it demonstrated optimal activity at 50 degrees C and pH 5.0. These findings suggest that Saccharomyces cerevisiae grown on cassava-soybean is a viable alternative for commercial invertase production.
SCIENTIFIC REPORTS
(2023)
Article
Multidisciplinary Sciences
Amira Hassan Alabdalall, Norah A. Al-Anazi, Lena A. Aldakheel, Fatma H. I. Amer, Fatimah A. Aldakheel, Ibtisam M. Ababutain, Azzah I. Alghamdi, Eida M. Al-Khaldi
Summary: This research conducted a biochemical characterization of lipase activity and stability for two strains of Aspergillus niger, identifying optimal conditions for enzyme activity under different temperatures and pH levels. Additionally, the study explored the effects of organic solvents, surfactants, and metal ions on lipase activity, providing insights for potential industrial applications. The results demonstrated that both strains exhibited robust lipase activity in detergent formulations, with promising potential for practical use in industrial settings.
SCIENTIFIC REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Deyaa Abol-Fotouh, Ola E. A. AlHagar, Mohamed A. Hassan
Summary: This study aimed to produce a highly compatible thermoalkaliphilic lipase using new thermophilic bacterial strains from fish wastes. The optimized fermentation parameters significantly increased the productivity of the TA lipase, which demonstrated outstanding activity and stability in extreme conditions. The purified enzyme showed high specific activity and fold purification, with potential for detergent formulations.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Biology
Shamsan A. Almowallad, Ghedeir M. Alshammari, Muneer M. Alsayadi, Naofel Aljafer, Ekram A. Al-Sanea, Mohammed Abdo Yahya, Laila Naif Al-Harbi
Summary: Pectinase enzymes are important industrial enzymes that are widely used in various industries, especially in food processing. This study successfully produced and purified exo-polygalacturonase (exo-PG) using local Penicillium oxalicum fungus grown on sugar beet waste. The purified exo-PG exhibited desirable biochemical properties for industrial application, making it a promising method for producing useful enzymes.
Article
Food Science & Technology
Wei Zhou, Cuizhu Ye, Lijing Geng, Guannan Chen, Xiaoyu Wang, Weijie Chen, Rina Sa, Junpeng Zhang, Xiang Zhang
Summary: Bromelain extracted from pineapple peel has potential applications in food industry and pharmaceutical products due to its antioxidant properties. Stability tests showed factors that inhibit or enhance the activity of purified bromelain, while kinetics study revealed the thermal inactivation of bromelain under different temperature conditions, aiding in predicting its inactivation time during food processing.
JOURNAL OF FOOD SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Yanan Li, Zongfu Chen, Peng Zhang, Feng Gao, Junfeng Wang, Li Lin, Haibin Zhang
Summary: In this study, a novel copper-zinc superoxide dismutase (CuZnSOD) was cloned and characterized from a new sea cucumber species Psychropotes verruciaudatus (PVCuZnSOD). The PVCuZnSOD showed optimum temperature at 20 degrees C and maintained high activity from 0-60 degrees C. It also exhibited strong tolerance to various ions and chemical reagents, indicating its potential applications in medicine, food, and other products.
Article
Biochemistry & Molecular Biology
Yonca Yuzugullu Karakus, Binnur Yildirim, Arda Acemi
Summary: Fennel seeds were identified as a promising source of polyphenol oxidase (PPO) and the purified PPO enzyme was characterized in detail for the first time. The enzyme showed the best activity towards the substrate catechol, suggesting potential industrial applications in producing low-cost enzymes. Inhibitors and organic solvents were found to impact PPO activity, with certain solvents maintaining the enzyme's activity at high concentrations.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Chemistry, Physical
Mohd Hadzdee Mohd Din, Anusha Nair, Malihe Masomian, Mohd Shukuri Mohamad Ali, Raja Noor Zaliha Raja Abd Rahman
Summary: The LIP2 gene of oil palm mesocarp lipase was isolated, expressed, purified and characterized. It showed distinctive lipolytic properties, optimal temperature and pH, increased stability with metal ions and organic solvents, and suitable substrate specificity. The thermal denaturing point of LIP2 was at 66 degrees C with a composition of random patterns, alpha-helix, turns, and beta-sheet. The successful purification and characterization of LIP2 allows for further exploration of its potential in industrial applications.
Article
Agricultural Engineering
Myeong-Eun Lee, Sang Kyu Shin, Jeong-Joo Oh, Dong-Hyeok Hwang, Young Jin Ko, Jeong Eun Hyeon, Sung Ok Han
Summary: CcGlcNAcase is a cellulosomal component that enhances the degradation of fungi-infected lignocellulosic biomass in the presence of chitin, leading to higher levels of glucose and total reducing sugars.
BIORESOURCE TECHNOLOGY
(2021)
Article
Medicine, General & Internal
Jeong-Ju Yoo, Jung Hyun Kwon, Young Seok Kim, Soon Woo Nam, Ji Won Park, Hee Yeon Kim, Chang Wook Kim, Seung Kak Shin, Young Eun Chon, Eun Sun Jang, Sook-Hyang Jeong, Jin Woo Lee, Do Seon Song, Jin Mo Yang, Sung Won Lee, Hae Lim Lee, Young Kul Jung, Hyung Joon Yim, Bora Lee, Sang Gyune Kim, Ju Hyun Kim
Summary: Urine NAG is associated with the severity of AKI in cirrhotic patients, and it is a useful predictor of 3-month transplant-free survival rate. However, it does not predict the response to terlipressin treatment in HRS patients.
JOURNAL OF CLINICAL MEDICINE
(2021)
Review
Biochemistry & Molecular Biology
F. Rafiee, M. Rezaee
Summary: Immobilized enzymes, particularly lipase on chitosan support, have attracted significant attention due to their advantages in industry and biochemistry. This review highlights the recent studies on lipase immobilization and discusses the potential solutions for current limitations.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Spectroscopy
Jiaxin Liu, Keke Ning, Yao Fu, Yujie Sun, Jiangong Liang
Summary: In this paper, a fluorescent sensor based on PEG-400 modified and H2O2-assisted etched SQDs for the fast and sensitive detection of NAG is developed. By utilizing the IFE, the fluorescence of SQDs can be quenched by PNP generated through NAG-catalyzed hydrolysis of PNP-NAG. The method demonstrates a detection range for NAG activity from 0.4 to 7.5 U center dot L-1 with a detection limit of 0.1 U center dot L-1, and it shows high selectivity in detecting NAG activity in bovine serum samples, indicating its potential in clinical detection.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2023)
Article
Pharmacology & Pharmacy
Oliwia Degorska, Daria Szada, Agata Zdarta, Wojciech Smulek, Teofil Jesionowski, Jakub Zdarta
Summary: In this study, lipase immobilized from Aspergillus niger by physical immobilization and mixed physical immobilization and ion exchange was used for kinetic resolution of the ketoprofen racemic mixture. The immobilized lipase showed improved activity and stability, and phosphate buffer at pH 7 was found to be the best solvent.
Article
Multidisciplinary Sciences
Gabriella Cavazzini Pavarina, Eliana Gertrudes de Macedo Lemos, Natalia Sarmanho Monteiro Lima, Joao Martins Pizauro Jr
Summary: Researchers identified a new bifunctional enzyme from the Nellore cattle rumen microbiota, which is stable and tolerant to various conditions and suitable for industrial environments. The enzyme shows potential for improving the degradation efficiency of heteroxylans and could be applied in lignocellulosic biomass processing and animal feed additives.
SCIENTIFIC REPORTS
(2021)
Review
Chemistry, Applied
Xingran Kou, Dongdong Su, Fei Pan, Xiwei Xu, Qingran Meng, Qinfei Ke
Summary: This review provides a systematic discussion of the application of molecular dynamics (MD) simulations in aroma compounds (ACs)/cyclodextrins (CDs) inclusion complexes (ICs). It covers the establishment of the simulation process, parameter selection, model evaluation, and various application cases, summarizing the major achievements and challenges of this method.
CARBOHYDRATE POLYMERS
(2024)
Review
Chemistry, Applied
Haoyu Yu, Quanbin Zhang, Ammad Ahmad Farooqi, Jing Wang, Yang Yue, Lihua Geng, Ning Wu
Summary: Brown algae are rich in fucoidan, which has been found to have anti-cancer and anti-metastasis effects. Fucoidan inhibits tumor cell growth, proliferation, and metastasis, and also promotes immune responses in the tumor microenvironment.
CARBOHYDRATE POLYMERS
(2024)
Review
Chemistry, Applied
Liang Dong, Yanan Li, Hailin Cong, Bing Yu, Youqing Shen
Summary: Gene therapy is a revolutionary treatment that requires suitable vectors for protecting and releasing exogenous nucleic acids in target cells. Chitosan, as a non-viral vector, has gained attention due to its good biocompatibility and ability to load large amounts of nucleic acids. This paper summarizes the potential of chitosan and its derivatives as gene delivery vector materials, discusses factors influencing transfection efficiency, performance evaluation, ways to optimize infectious efficiency, and current research development directions. It also provides an outlook on the future prospects of chitosan.
CARBOHYDRATE POLYMERS
(2024)
Article
Chemistry, Applied
Ana Iglesias-Mejuto, Beatriz Magarinos, Tania Ferreira-Goncalves, Ricardo Starbird-Perez, Carmen Alvarez-Lorenzo, Catarina Pinto Reis, Ines Ardao, Carlos A. Garcia-Gonzalez
Summary: This study developed a novel processing strategy to manufacture drug-loaded and personalized aerogels with nanostructures. The aerogels demonstrated bioactivity and antimicrobial effects, promoting bone regeneration and preventing infections in bone tissue engineering.
CARBOHYDRATE POLYMERS
(2024)
Article
Chemistry, Applied
Zhenxu Shi, Dimei Yang, Yan Zhou, Xinyu Chen, Lin Gan, Jin Huang
Summary: This study proposes a micro-assembly method to improve the photoluminescent properties of crystalline cellulose nanocrystals (CNCs) by organizing them within a sub-micrometer-sized metal-organic framework and coating with TiO2. The TiO2 coating prevents CNC assembly breakdown and allows information to be revealed using screenprinted labels for anti-counterfeiting purposes.
CARBOHYDRATE POLYMERS
(2024)
Article
Chemistry, Applied
Xuejiao Liu, Bin Fu, Jierong Chen, Zhenyu Sun, Dongdong Zheng, Zhonghua Li, Bing Gu, Ying Zhang, Haojie Lu
Summary: Liver disease is a major cause of global mortality, and identifying biomarkers for diagnosing its progression is crucial for improving outcomes. Targeted mass spectrometry is a powerful tool for verifying biomarker candidates and clinical applications, particularly for glycoproteins translation. However, the limitation of analyzing only one sample per run has become apparent. In this study, a high-throughput intact N-glycopeptides quantification strategy was developed, allowing the validation of 20 samples per run with an average analysis time of 3 minutes per sample. The strategy was applied in a cohort of 461 serum samples and identified a panel of 10 IgG N-glycopeptides that have strong clinical utility in evaluating the severity of liver disease.
CARBOHYDRATE POLYMERS
(2024)
Article
Chemistry, Applied
Qian Wang, Hao Jiang, Hongli Zhang, Weiqiao Lu, Xiao Wang, Wenfeng Xu, Jia Li, Youjing Lv, Guoyun Li, Chao Cai, Guangli Yu
Summary: This study proposes a novel strategy of antibody-beta-glucan conjugates (AGC) to enhance the antitumor immune response to immune checkpoint blockade (ICB) therapy. AGC demonstrated powerful tumor suppression and promoted interaction between tumor cells and dendritic cells (DCs), thereby enhancing immunotherapeutic benefits.
CARBOHYDRATE POLYMERS
(2024)
Article
Chemistry, Applied
Chaeyeon Lee, Sojeong Lee, Sang Wook Kang
Summary: The study aims to enhance the properties of porous membranes by addressing the limitations associated with phase separation. By using cellulose acetate and citric acid, the researchers were able to fabricate membranes with improved mechanical strength and thermal stability. The cross-linking effect of citric acid resulted in a more uniform pore structure and higher porosity.
CARBOHYDRATE POLYMERS
(2024)
Review
Chemistry, Applied
Tejaswini Kolipaka, Giriraj Pandey, Noella Abraham, Dadi A. Srinivasarao, Rajeev Singh Raghuvanshi, P. S. Rajinikanth, Vidya Tickoo, Saurabh Srivastava
Summary: This review focuses on the design and application of polysaccharide-based hydrogel wound dressings, highlighting aspects such as biocompatibility, biodegradability, drug entrapment, moisturizing ability, swelling, and mechanical properties. Additionally, various crosslinking methods and recent developments in stimuli-responsive hydrogels are discussed.
CARBOHYDRATE POLYMERS
(2024)
Article
Chemistry, Applied
Anqi Xu, Nan Zhang, Shixing Su, Hongyu Shi, Daoqiang Lu, Xifeng Li, Xin Zhang, Xin Feng, Zhuohua Wen, Gengwu Ma, Mengshi Huang, Chi Huang, Yuqi Hu, Hao Yuan, Qinwen Liu, Daogang Guan, Jun Wang, Chuanzhi Duan
Summary: The study presents a one-pot radical polymerization method to fabricate a hydrogel with adhesive properties, which can effectively treat bacterial-infected diabetic wounds and accelerate wound healing.
CARBOHYDRATE POLYMERS
(2024)
Article
Chemistry, Applied
Montarop Yamabhai, Munthipha Khamphio, Thae Thae Min, Chai Noy Soem, Nguyen Cao Cuong, Waheni Rizki Aprilia, Krisanai Luesukprasert, Karsidete Teeranitayatarn, Atthaphon Maneedaeng, Tina R. Tuveng, Silje B. Lorentzen, Simen Antonsen, Paiboon Jitprasertwong, Vincent G. H. Eijsink
Summary: The study investigates the bioconversion of chitosan into soluble anti-inflammatory chitosan oligosaccharides (CHOS) using an enzyme. The results show that the generated CHOS have anti-inflammatory activity, but the magnitude of the activity depends on the substrate and production process. Different methods of dissolving chitosan also affect the properties of CHOS. The study highlights the importance of quality assurance in CHOS preparations.
CARBOHYDRATE POLYMERS
(2024)
Article
Chemistry, Applied
Wen Cao, Jiangping Wu, Xinya Zhao, Zixu Li, Jie Yu, Taili Shao, Xuefeng Hou, Lutan Zhou, Chunfei Wang, Guodong Wang, Jun Han
Summary: In this study, a water-soluble polysaccharide (PLP1) was successfully isolated and purified from Pueraria lobata. It was found that PLP1 was composed of specific glycosidic units and exhibited a better free radical-scavenging ability. Moreover, PLP1 effectively protected the liver against acute alcoholic liver disease (ALD) in mice.
CARBOHYDRATE POLYMERS
(2024)
Article
Chemistry, Applied
Ya Su, Yaqian Liu, Xueyan Hu, Yueqi Lu, Jinyuan Zhang, Wenbo Jin, Wang Liu, Yan Shu, Yuen Yee Cheng, Wenfang Li, Yi Nie, Bo Pan, Kedong Song
Summary: A 3D printed scaffold based on carbon dots-curcumin nano-drug release has been developed for drug delivery after breast cancer surgery. The scaffold showed effective inhibition of tumor growth, antibacterial activity, and promotion of wound healing, making it a promising approach for preventing tumor recurrence.
CARBOHYDRATE POLYMERS
(2024)
Article
Chemistry, Applied
Ekaterina Sokolova, Diane Jouanneau, Antonin Chevenier, Murielle Jam, Nathalie Desban, Pierre Colas, Elizabeth Ficko-Blean, Gurvan Michel
Summary: Carrageenans, a compound synthesized in red algae, have various biological properties and are valuable in the pharmaceutical and cosmetic industries. Their fine structure affects wound healing, oxidative processes, hemostasis, and inflammation. Enzymatic modification of carrageenans produces oligosaccharides that bind to natural human serum antibodies and specific antibodies, showing potential for therapeutic applications.
CARBOHYDRATE POLYMERS
(2024)
Article
Chemistry, Applied
Yan Shi, Miao Yu, Kaijin Qiu, Tiantian Kong, Chunjing Guo, Wenxue Zhang, Daquan Chen, Ming Kong
Summary: In this study, functionalized transfersomes were developed to co-deliver doxorubicin and 1MT towards primary tumors and tumor draining lymph nodes via transdermal administration using microneedles. The results showed that the nano-complexed microneedles exhibited a stronger suppression in tumor growth compared to the intravenous group.
CARBOHYDRATE POLYMERS
(2024)