Review
Biochemistry & Molecular Biology
Cailin O'Connell, Sabrina VandenHeuvel, Aparna Kamat, Shreya Raghavan, Biana Godin
Summary: This review provides an overview of the clinical features and diagnostic importance of ovarian cancer, focusing on the role of proteases in its progression and the potential of protease-enabled nanomedicines for diagnosis and therapy.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Cynthia Buelck, Elisabeth E. L. Nystroem, Tomas Koudelka, Michael Mannbar-Frahm, Gerrit Andresen, Mariem Radhouani, Florian Tran, Franka Scharfenberg, Friederike Schrell, Fred Armbrust, Eileen Dahlke, Bei Zhao, Alex Vervaeke, Franziska Theilig, Philip Rosenstiel, Philipp Starkl, Stephan P. Rosshart, Helmut Fickenscher, Andreas Tholey, Gunnar C. Hansson, Christoph Becker-Pauly
Summary: The metalloproteases meprin alpha and meprin beta are highly expressed in the healthy gut but significantly decreased in inflammatory bowel disease. They form covalently linked heterodimers in the colon, and the major intestinal substrate for these heterodimers is galectin-3. The cleavage of galectin-3 by meprin alpha/beta heterodimers may play a key role in colon host-microbiome homeostasis.
Review
Chemistry, Analytical
Jin-Ha Choi
Summary: Proteolytic enzymes are important biomarkers for early diagnosis of diseases such as cancer. By selectively degrading specific sequences of polypeptides, these enzymes can be quantified by changing detectable signals causing degradation of the peptide chain. Combining polypeptides with functional nanomaterials allows for more sensitive and rapid measurement of proteolytic enzymes. This paper reviews proteolytic enzymes measured using a polypeptide degradation method and discusses recently studied functional nanomaterials-based proteolytic biosensors. The proteolytic nanobiosensors discussed in this review are expected to provide valuable information on physiological changes from a cellular level for individual and early diagnosis.
Review
Biochemistry & Molecular Biology
Preston Carey, Ethan Low, Elizabeth Harper, M. Sharon Stack
Summary: Proteases, particularly those of the matrix metalloproteinase family, play a crucial role in ovarian cancer progression and metastasis by enhancing migratory ability, ECM breakdown, and tumor support system development. Upregulation of certain proteases influences angiogenesis for tumor growth and development of metastatic implants.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Endocrinology & Metabolism
Christoph Klenk, Leif Hommers, Martin J. Lohse
Summary: This study reveals that the extracellular domain of PTH1R is cleaved by metalloproteinases, leading to impaired stability of the receptor. The cleavage site is located in the first loop of the extracellular domain. A receptor mutant resistant to proteolytic cleavage showed reduced signaling to G(s) and increased activation of G(q).
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Entomology
Yi Wei, Xiao-Lin Zhou, Peng Chen, Tai-Hang Liu, Cheng Lu, Min-Hui Pan
Summary: The study found that matrix metalloproteinase 2 (MMP2) is highly expressed in the ovarian capsules and ovarioles of B. mori, and knockout of MMP2 leads to abnormal ovarian development. The abnormal degradation of collagen I due to MMP2 deletion results in abnormal development of ovarioles and eggs. Furthermore, insulin-like peptide (ILP) expression also undergoes significant changes during ovarian development, and abnormal expression of ILP significantly affects ovarian development and MMP2 expression.
Article
Biochemical Research Methods
Nisitha Wijewantha, Morgan M. Eikanger, Ryan M. Antony, Rashaun A. Potts, Khosrow Rezvani, Grigoriy Sereda
Summary: The study aimed to develop a therapeutic strategy using nanoparticles to selectively deliver the anticancer molecule VTD to colorectal cancer cells and release it on demand, enhancing efficiency through enzyme-triggered release with minimal side effects, making it a promising anticancer drug.
BIOCONJUGATE CHEMISTRY
(2021)
Article
Medicine, General & Internal
Jane B. Maoga, Muhammad A. Riaz, Agnes N. Mwaura, Georgios Scheiner-Bobis, Ezekiel Mecha, Charles O. A. Omwandho, Ivo Meinhold-Heerlein, Lutz Konrad
Summary: Matrix metalloproteinases (MMPs) play an important role in menstruation and endometriosis. The expression of MT2-MMP and MT3-MMP proteins varies in different types of endometrial tissues.
Article
Immunology
Jennifer Vandooren, Rafaela Vaz Sousa Pereira, Estefania Ugarte-Berzal, Vasily Rybakin, Sam Noppen, Melissa R. Stas, Eline Bernaerts, Eva Ganseman, Mieke Metzemaekers, Dominique Schols, Paul Proost, Ghislain Opdenakker
Summary: Interleukin 7 (IL-7) plays a crucial role in T cell development, cancer, and autoimmune diseases. Abnormalities in IL-7 signaling are associated with T cell acute lymphoblastic leukemia (T-ALL) and involve interactions with matrix metalloproteinase-9 (MMP-9).
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Amit Itzhar, Gal Yosef, Maayan Eilon-Ashkenazy, Yulia Shmidov, Hadas Gil, Shiran Lacham-Hartman, Sigal Elyagon, Sharon Etzion, Ronit Bitton, Smadar Cohen, Yoram Etzion, Niv Papo
Summary: Sustained drug-release systems prolong the retention of therapeutic drugs within target tissues, reducing the need for repeated drug administration. The presented system provides controlled and universal drug-release by binding therapeutic proteins to alginate sulfate through heparin-binding peptide repeats.
JOURNAL OF CONTROLLED RELEASE
(2023)
Review
Biochemistry & Molecular Biology
Karina Reiss, Sinje Leitzke, Jana Seidel, Maria Sperrhacke, Sucharit Bhakdi
Summary: Proteolytic ectodomain release is a crucial mechanism for regulating cell surface protein function, with ADAM10 and ADAM17 as key players. Recent research suggests that the cell membrane itself plays a critical role in upregulating sheddase function. This article summarizes the current knowledge on cell membrane changes, phosphatidylserine exposure, and proteolytic activity of transmembrane proteases, as well as their potential consequences in immune response, infection, and cancer.
Review
Cell & Tissue Engineering
Priscila Anhel Medrano-Gonzalez, Osmar Rivera-Ramirez, Luis Felipe Montano, Erika P. Rendon-Huerta
Summary: CD44 is a transmembrane glycoprotein expressed in healthy and tumor tissues, with modifications in its structure affecting its activity, particularly through consecutive cleavage of its extracellular ectodomain by metalloproteases. This process generates biologically active substrates, contributing to the regulation of genes and proteins associated with cancer development.
STEM CELLS INTERNATIONAL
(2021)
Article
Multidisciplinary Sciences
Wei-Yun Shen, Hui Li, An-Hui Zha, Ru-Yi Luo, Yan-Ling Zhang, Cong Luo, Ru-Ping Dai
Summary: Platelets have the ability to modulate immune responses and are associated with cardiac disease. Despite low platelet counts, platelets in patients with acute aortic dissection (AAD) are activated, leading to significant alterations in immune-modulating mediators. Monocytes in AAD patients have suppressed immune status, which correlates with poor outcomes following surgery. Platelets preferentially aggregate with monocytes, and the levels of monocyte-platelet aggregates (MPAs) are related to postoperative recovery. Platelets restore suppressed monocyte functions by forming aggregates and secreting matrix metalloproteinase-9 (MMP-9), potentially improving postoperative outcomes following complex cardiovascular surgery.
Review
Plant Sciences
Jonas De Backer, Frank Van Breusegem, Inge De Clercq
Summary: This review focuses on the proteolytic activation mechanisms of membrane-bound or membrane-tethered transcription factors (MB-TFs) in plants, particularly regulated intramembrane proteolysis (RIP). By mapping protease recognition sequences and structural features, proteolytically activated MB-TFs are predicted. The functions of MB-TFs in plant responses to environmental stresses and plant development are considered.
FRONTIERS IN PLANT SCIENCE
(2022)
Review
Biochemistry & Molecular Biology
Eva Nagyova, Lucie Nemcova, Antonella Camaioni
Summary: The extracellular matrix plays a crucial role in the development of ovarian follicles, and its formation and function are regulated by proteolytic cleavage and hormonal stimulation. Specifically, the proteasome inhibitor MG132 affects the cytoskeletal structure, organization of cumulus ECM, steroidogenesis, and nuclear maturation in gonadotropin-stimulated oocyte-cumulus complexes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Ryo Iwamoto, Naoki Mine, Hiroto Mizushima, Eisuke Mekada
JOURNAL OF CELL SCIENCE
(2017)
Article
Biochemistry & Molecular Biology
Takayo Takemura, Yuichi Yoshida, Shinichi Kiso, Takashi Kizu, Kunimaro Furuta, Hisao Ezaki, Mina Hamano, Mayumi Egawa, Norihiro Chatani, Yoshihiro Kamada, Yasuharu Imai, Shigeki Higashiyama, Ryo Iwamoto, Eisuke Mekada, Tetsuo Takehara
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2013)
Article
Biochemistry & Molecular Biology
Ayaka Okamoto, Tomohiro Asai, Hiroki Kato, Hidenori Ando, Tetsuo Minamino, Eisuke Mekada, Naoto Oku
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2014)
Article
Oncology
Takuya Murata, Hiroto Mizushima, Ichino Chinen, Hiroki Moribe, Shigeo Yagi, Robert M. Hoffman, Tadashi Kimura, Kiyoshi Yoshino, Yutaka Ueda, Takayuki Enomoto, Eisuke Mekada
Article
Oncology
Shingo Miyamoto, Ryo Iwamoto, Akiko Furuya, Kumiko Takahashi, Yuka Sasaki, Hiroshi Ando, Fusanori Yotsumoto, Tomoko Yoneda, Miki Hamaoka, Hiroshi Yagi, Takuya Murakami, Sayaka Hori, Kenya Shitara, Eisuke Mekada
CLINICAL CANCER RESEARCH
(2011)
Article
Biochemistry & Molecular Biology
Hitomi Masuda, Kuniaki Nakamura, Nozomu Takata, Bunsho Itoh, Takashi Hirose, Hiroki Moribe, Eisuke Mekada, Masato Okada
Article
Gastroenterology & Hepatology
Takayo Takemura, Yuichi Yoshida, Shinichi Kiso, Yukiko Saji, Hisao Ezaki, Mina Hamano, Takashi Kizu, Mayumi Egawa, Norihiro Chatani, Kunimaro Furuta, Yoshihiro Kamada, Ryo Iwamoto, Eisuke Mekada, Shigeki Higashiyama, Norio Hayashi, Tetsuo Takehara
HEPATOLOGY RESEARCH
(2013)
Article
Biochemistry & Molecular Biology
Keisuke Suzuki, Hiroto Mizushima, Hiroyuki Abe, Ryo Iwamoto, Haruki Nakamura, Eisuke Mekada
JOURNAL OF BIOCHEMISTRY
(2015)
Article
Biochemistry & Molecular Biology
Elena Odintsova, Guillaume van Niel, Helene Conjeaud, Graca Raposo, Ryo Iwamoto, Eisuke Mekada, Fedor Berditchevski
JOURNAL OF BIOLOGICAL CHEMISTRY
(2013)
Article
Chemistry, Multidisciplinary
Kaoru Nishikawa, Tomohiro Asai, Hirokazu Shigematsu, Kosuke Shimizu, Hisakazu Kato, Yoshihiro Asano, Seiji Takashima, Eisuke Mekada, Naoto Oku, Tetsuo Minamino
JOURNAL OF CONTROLLED RELEASE
(2012)
Article
Genetics & Heredity
Hiroki Moribe, Ryouji Konakawa, Daisuke Koga, Tatsuo Ushiki, Kuniaki Nakamura, Eisuke Mekada
Article
Oncology
Sung Ouk Nam, Fusanori Yotsumoto, Kohei Miyata, Yuki Suzaki, Hiroshi Yagi, Takashi Odawara, Sadao Manabe, Toyokazu Ishikawa, Masahide Kuroki, Eisuke Mekada, Shingo Miyamoto
ANTICANCER RESEARCH
(2014)
Article
Oncology
Noriyuki Kasai, Kazuhiro Kobayashi, Shinobu Shioya, Yukitaka Yoshikawa, Fusanori Yotsumoto, Shingo Miyamoto, Eisuke Mekada, Junichi Enokizono
AMERICAN JOURNAL OF TRANSLATIONAL RESEARCH
(2012)
Article
Oncology
Shoko Hikita, Fusanori Yotsumoto, Tatsuya Fumaki, Shinji Horiuchi, Ayako Sanui, Kohei Miyata, Sung Ouk Nam, Hiroshi Tsujioka, Taeko Ueda, Kyoko Shirota, Toshiyuki Yoshizato, Kazuhiro Maeda, Toyokazu Ishikawa, Yoshinobu Okuno, Masahide Kuroki, Eisuke Mekada, Shingo Miyamoto
ANTICANCER RESEARCH
(2011)