Article
Biochemistry & Molecular Biology
Saadiya Zia, Komal Tehreem, Sidra Batool, Mehreen Ishfaq, Shaher Bano Mirza, Shahrukh Khan, Majed N. Almashjary, Mohannad S. Hazzazi, Husam Qanash, Ahmad Shaikh, Roua S. Baty, Ibrahim Jafri, Nouf H. Alsubhi, Ghadeer I. Alrefaei, Rokayya Sami, Ramla Shahid
Summary: This study found that the expression of EpCAM in cancer cells was inhibited by costunolide, suggesting that costunolide could be a potential therapeutic biomolecule for leukemia.
Article
Oncology
Mustafa Karabicici, Sena Alptekin, Zeynep Firtina Karagonlar, Esra Erdal
Summary: Therapeutic induction of senescence may increase cancer stem cell phenotype, leading to increased tumor aggressiveness and disease recurrence. In liver cancer cells, doxorubicin can induce cellular senescence, increase stem cell gene expression, and cause a shift in stem cell characteristics.
MOLECULAR ONCOLOGY
(2021)
Article
Veterinary Sciences
Christa Y. Heyward, Lynn Dong, Hayk Shakhzadyan, Christopher Wan, Tracy Stokol
Summary: The study found that EpCAM is expressed in specific epithelia in cats and is variably expressed in feline mammary tumors and oropharyngeal squamous cell carcinoma. A higher avidity cross-reactive or feline-specific antibody is needed for further investigation of EpCAM expression in feline tissue or for detecting CTCs in the blood of tumor-bearing cats.
FRONTIERS IN VETERINARY SCIENCE
(2021)
Article
Multidisciplinary Sciences
Anouk Mentink, Khrystany T. Isebia, Jaco Kraan, Leon W. M. M. Terstappen, Michiel Stevens
Summary: When evaluating EpCAM-based enrichment technologies for CTCs, it is important to use cell lines that closely resemble real CTCs and measure the EpCAM expression of both CTCs and cell lines at different institutions and times. In this study, CTCs were enriched by depleting leukocytes from diagnostic leukapheresis products and EpCAM expression was measured using quantitative flow cytometry. Results showed varying but relatively low EpCAM expression in CTCs from castration-sensitive prostate cancer patients. There were also significant variations in antigen expression among identical cell lines cultured at different institutions, affecting capture efficiency.
SCIENTIFIC REPORTS
(2023)
Article
Medicine, Research & Experimental
Chi-Chiu Lee, Chia-Jui Yu, Sushree Shankar Panda, Kai-Chi Chen, Kang-Hao Liang, Wan-Chen Huang, Yu-Shiuan Wang, Pei-Chin Ho, Han-Chung Wu
Summary: This study explores the interaction between EpCAM and HGFR and its impact on the metastatic and invasive abilities of colon cancer cells. The results demonstrate that EpCAM binds to HGFR and activates the ERK and FAK-AKT signaling pathways, leading to the promotion of colon cancer metastasis. Furthermore, the combined treatment with EpCAM and HGFR antibodies significantly inhibits tumor progression and improves patient survival.
JOURNAL OF TRANSLATIONAL MEDICINE
(2023)
Review
Biochemistry & Molecular Biology
Taylor C. Brown, Narendra Sankpal, William E. Gillanders
Summary: Epithelial cell adhesion molecule (EpCAM) is a transmembrane glycoprotein that plays a crucial role in epithelial tissue homeostasis and is a target of molecular therapies for tumor biology. Its expression is frequently high in epithelial cancers and silenced in mesenchymal cancers. EpCAM's functional impact on tumor biology is multifunctional and depends on the predominant oncogenic signaling pathways in different cancer types. Its regulation during epithelial-to-mesenchymal transition (EMT) involves downregulation by extracellular signal-regulated kinases (ERK) and EMT transcription factors, as well as regulated intramembrane proteolysis (RIP).
Article
Multidisciplinary Sciences
Yang Gao, Wan-Hung Fan, Zhengbo Song, Haizhou Lou, Xixong Kang
Summary: By comparing the CTC detection rates with EpCAM and CSV antibodies in different solid tumors, it was found that EpCAM has higher detection rates in colorectal cancer, while CSV performs better in sarcoma.
Article
Oncology
Chunjin Huang, Sheng Ding, Chunyan Huang, Feng Pan, Xiaodong Liu, Haijiao Zhang, Jian Zhou, Xiaofei Liang, Xinyan Wang, Ping Song
Summary: The study aimed to determine the count of Circulating Tumor Cells (CTCs) in peripheral blood from high-risk population, healthy subjects, and patients with Pan-cancer. Results showed significant differences in CTCs count among healthy subjects, high-risk population, and total cancer patients, with a notable impact on the survival time of cancer patients.
FRONTIERS IN ONCOLOGY
(2021)
Article
Gastroenterology & Hepatology
Taro Yamashita, Naohiko Koshikawa, Tetsuro Shimakami, Takeshi Terashima, Masatoshi Nakagawa, Kouki Nio, Rika Horii, Noriho Iida, Kazunori Kawaguchi, Kuniaki Arai, Yoshio Sakai, Tatsuya Yamashita, Eishiro Mizukoshi, Masao Honda, Azusa Kitao, Satoshi Kobayashi, Shizuko Takahara, Yasuhito Imai, Kenichi Yoshimura, Toshinori Murayama, Yasunari Nakamoto, Eisaku Yoshida, Toru Yoshimura, Motoharu Seiki, Shuichi Kaneko
Summary: The study found that the LG2m gene signature is associated with poor prognosis in hepatocellular carcinoma (HCC) patients, and elevated serum LG2m may be a risk factor for HCC development. In chronic hepatitis C (CHC) patients who have achieved sustained virological response (SVR), elevated serum LG2m is correlated with a high incidence of HCC.
Article
Multidisciplinary Sciences
Cecile Gaston, Simon De Beco, Bryant Doss, Meng Pan, Estelle Gauquelin, Joseph D'Alessandro, Chwee Teck Lim, Benoit Ladoux, Delphine Delacour
Summary: The study highlights the importance of EpCAM/RhoA co-trafficking and actomyosin rearrangement in cell spreading and polarization, furthering our understanding of how biochemical and mechanical properties contribute to cell plasticity.
NATURE COMMUNICATIONS
(2021)
Article
Oncology
Yanping Yang, Raymond Louie, Janusz Puc, Yogindra Vedvyas, Yago Alcaina, Irene M. Min, Matt Britz, Fabio Luciani, Moonsoo M. Jin
Summary: This study investigates the interactions between CAR T cells targeting EpCAM and tumor cells in a xenograft model of gastric cancer. The research reveals that within resistant tumors, CAR T cells proliferate but become largely dysfunctional, and CD4 T cells transform into regulatory T cells. The resistant tumor cells also exhibit specific gene changes that promote cancer growth. The study provides valuable insights into the mechanisms of resistance to CAR T cell therapy and may guide future developments in cancer treatment.
Article
Oncology
Han Chen, Kouki Nio, Taro Yamashita, Hikari Okada, Ru Li, Tsuyoshi Suda, Yingyi Li, Phuong Thi Bich Doan, Akihiro Seki, Hidetoshi Nakagawa, Tadashi Toyama, Takeshi Terashima, Noriho Iida, Tetsuro Shimakami, Hajime Takatori, Kazunori Kawaguchi, Yoshio Sakai, Tatsuya Yamashita, Eishiro Mizukoshi, Masao Honda, Shuichi Kaneko
Summary: The presence of cancer stem cells in hepatocellular carcinoma is closely related to bone morphogenic protein 9 (BMP9) signaling. High BMP9 expression leads to poorer outcomes in HCC patients, while BMP9 promotes CSC properties in a specific subtype of HCC. Inhibitors of BMP receptors have therapeutic potential in treating HCC by blocking BMP9 signaling and suppressing CSC activation.
MOLECULAR ONCOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Wei-Che Yen, Qiao-Zhu Li, Li-Hsien Wu, Wei-Ya Lee, Wen-Wei Chang, Peng-Ju Chien, Che-Hsin Lee
Summary: EpCAM plays a crucial role in tumor metastasis and progression by regulating epithelial-mesenchymal transition and inducing stem cell-like phenotype. Salmonella-based treatments show potential in inhibiting tumor metastasis through downregulation of EpCAM and inhibition of the AKT/mTOR signaling pathway.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Biochemistry & Molecular Biology
Yoshiro Asahina, Hajime Takatori, Kouki Nio, Hikari Okada, Takehiro Hayashi, Tomoyuki Hayashi, Tomomi Hashiba, Tsuyoshi Suda, Masaki Nishitani, Saiho Sugimoto, Masao Honda, Shuichi Kaneko, Taro Yamashita
Summary: This study found that dUTPase is expressed abundantly in EpCAM+ HCC cells and β-HIVS can decrease dUTPase expression and inhibit the proliferation of liver CSCs.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Kei Takuma, Shintaro Fujihara, Koji Fujita, Hisakazu Iwama, Mai Nakahara, Kyoko Oura, Tomoko Tadokoro, Shima Mimura, Joji Tani, Tingting Shi, Asahiro Morishita, Hideki Kobara, Takashi Himoto, Tsutomu Masaki
Summary: This study investigates the antitumor mechanism of regorafenib on hepatocellular carcinoma (HCC) and the impact on microRNA (miRNA) expression. The results suggest that regorafenib inhibits cell proliferation and tumor growth in HCC by altering the cell cycle and miRNA expression, particularly downregulating cyclin D1.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Medicine, General & Internal
Hideto Kawaratani, Yasuteru Kondo, Ryoji Tatsumi, Naoto Kawabe, Norikazu Tanabe, Akira Sakamaki, Kazuo Okumoto, Yoshihito Uchida, Kei Endo, Takumi Kawaguchi, Tsunekazu Oikawa, Yoji Ishizu, Shuhei Hige, Taro Takami, Shuji Terai, Yoshiyuki Ueno, Satoshi Mochida, Yasuhiro Takikawa, Takuji Torimura, Tomokazu Matsuura, Masatoshi Ishigami, Kazuhiko Koike, Hitoshi Yoshiji
Summary: This study found that long-term treatment with rifaximin is effective and safe for patients with hepatic encephalopathy. Ammonia levels significantly improved after 3 months of treatment and remained improved until 12 months. There were no serious adverse events reported, and the incidence of overt hepatic encephalopathy was low.
JOURNAL OF CLINICAL MEDICINE
(2022)
Article
Gastroenterology & Hepatology
Toshiya Machida, Taku Obara, Makoto Miyazaki, Jun Inoue, Nariyasu Mano
Summary: Patients with comorbid non-alcoholic fatty liver disease (NAFLD) and/or non-alcoholic steatohepatitis (NASH) tend to be prescribed specific drugs for type 2 diabetes, hypertension, and dyslipidemia. However, the differences in prescription ratios are not considerable, and further investigation is needed to confirm the effects of these drugs on the prognosis of patients with NAFLD or NASH.
ANNALS OF HEPATOLOGY
(2022)
Article
Gastroenterology & Hepatology
Kazumasa Sakamoto, Kiyoaki Ito, Hiroshi Yotsuyanagi, Hiroshi Yatsuhashi, Yasuhito Tanaka, Shuhei Hige, Yasuhiro Takikawa, Yoshiyuki Ueno, Kazuhide Yamamoto, Fumio Imazeki, Jun Inoue, Masayuki Kurosaki, Takeji Umemura, Hidenori Toyoda, Eiji Mita, Kojiro Michitaka, Tatsuji Maeshiro, Norie Yamada, Atsushi Suetsugu, Miwa Kawanaka, Yuya Seko, Kentaro Matsuura, Akinori Okumura, Yoshitaka Fukuzawa, Masaya Sugiyama, Masashi Mizokami, Masashi Yoneda
Summary: This study found that the distribution of HBV genotypes among CHB patients in Japan has changed over time, with an increase in genotype A among young adults and a shift of genotypes B and C towards older age groups. This data provides important baseline information for comparative studies on the impact of universal HBV vaccination.
JOURNAL OF GASTROENTEROLOGY
(2022)
Letter
Gastroenterology & Hepatology
Y. Sasaki, Y. Abe, S. Nishise, Y. Ueno
JOURNAL OF GASTROENTEROLOGY AND HEPATOLOGY
(2022)
Article
Gastroenterology & Hepatology
Toshiya Machida, Taku Obara, Mami Ishikuro, Keiko Murakami, Fumihiko Ueno, Aoi Noda, Tomomi Onuma, Fumiko Matsuzaki, Jun Inoue, Shinichi Kuriyama, Nariyasu Mano
Summary: This study aimed to examine the association between liver steatosis and fibrosis and atherosclerosis and chronic kidney disease. The results showed that a higher fatty liver index was associated with chronic kidney disease, independent of other risk factors. Further research is needed to understand the causal relationship between liver fat accumulation and chronic kidney disease.
ANNALS OF HEPATOLOGY
(2023)
Article
Medicine, General & Internal
Mio Tsuruoka, Jun Inoue, Keishi Ouchi, Kaname Uno, Hideaki Itami, Masashi Ninomiya, Tomoaki Iwata, Akitoshi Sano, Kosuke Sato, Masazumi Onuki, Satoko Sawahashi, Tomoyuki Koike, Atsushi Masamune
Summary: We report a rare case of duodenal varices rupture in an 87-year-old man during chemotherapy with atezolizumab and bevacizumab (ATZ/BV) for hepatocellular carcinoma. Emergency esophagogastroduodenoscopy (EGD) revealed massive bleeding of a ruptured varix in the horizontal portion of the duodenum. Successful hemostasis was achieved through endoscopic injection sclerotherapy. Although esophageal varices rupture has been reported with ATZ/BV treatment, this is the first case of duodenal varices rupture. Regular evaluation of both duodenal and esophageal varices is recommended before ATZ/BV treatment.
Article
Multidisciplinary Sciences
Kaori Sakurada, Tsuneo Konta, Narumi Murakami, Naoko Kosugi, Takafumi Saito, Masafumi Watanabe, Kenichi Ishizawa, Yoshiyuki Ueno, Takamasa Kayama
Summary: This study found that lack of sexual interest may be a risk factor for all-cause mortality in Japanese males over 40 years old.
Article
Medical Laboratory Technology
Akihiro Saito, Masafumi Kikuchi, Yuko Matsumoto, Erina Sugawara, Gesshu Takao, Hayato Inomata, Akane Takahashi, Yuji Sato, Masaki Kumondai, Yu Sato, Toshihiro Sato, Masashi Ninomiya, Jun Inoue, Masamitsu Maekawa, Nariyasu Mano
Summary: A novel liquid chromatography-electrospray ionization tandem mass spectrometry method was developed to accurately measure lenvatinib in human plasma, and it demonstrated good reproducibility. Therefore, MSW combined with this new detection method could serve as a useful microsampling device for lenvatinib therapeutic drug monitoring in patients with HCC.
THERAPEUTIC DRUG MONITORING
(2022)
Review
Medicine, General & Internal
Yu Sasaki, Yasuhiko Abe, Naoko Mizumoto, Eiki Nomura, Yoshiyuki Ueno
Summary: Eosinophilic gastroenteritis (EoGE) is a rare digestive disorder characterized by eosinophilic infiltration in the stomach and intestines. The endoscopic findings of EoGE in the small intestine are not well understood, but this literature review identified common findings such as redness, villous atrophy, erosion, ulceration, edema, and stenosis. These findings contribute to the development of EoGE practice.
Review
Medicine, General & Internal
Yasuhiko Abe, Yu Sasaki, Makoto Yagi, Naoko Mizumoto, Yusuke Onozato, Matsuki Umehara, Yoshiyuki Ueno
Summary: Eosinophilic esophagitis is a chronic immune-mediated inflammatory disease characterized by esophageal dysfunction and intense eosinophil infiltration. The recognition of characteristic endoscopic features and subsequent biopsy are crucial for early diagnosis and treatment, but the diagnostic consistency among endoscopists is still inadequate. Image-enhanced endoscopy holds promise for improving diagnostic efficiency.
Article
Pharmacology & Pharmacy
Junji Furuse, Namiki Izumi, Kenta Motomura, Yoshitaka Inaba, Yoshio Katamura, Yasuteru Kondo, Kazuhisa Yabushita, Katsuaki Motoyoshi, Masatoshi Kudo
Summary: This study aimed to evaluate the real-world safety and effectiveness of Lenvatinib for unresectable hepatocellular carcinoma (uHCC) in clinical practice in Japan. Data of 703 uHCC patients were analyzed, and it was found that the treatment response rate and median overall survival of Lenvatinib were similar to or better than the results of the global Phase III REFLECT trial.
DRUGS-REAL WORLD OUTCOMES
(2023)
Article
Biochemistry & Molecular Biology
Shinpei Sugahara, Hiroaki Haga, Chisaki Ikeda, Naohiko Makino, Akiko Matsuda, Yasuharu Kakizaki, Kyoko Hoshikawa, Tomohiro Katsumi, Tetsuya Ishizawa, Toshikazu Kobayashi, Keita Maki, Fumiya Suzuki, Ryoko Murakami, Hidenori Sato, Yoshiyuki Ueno
Summary: Despite extensive study on liver regeneration, the impact of bile-derived extracellular vesicles (bile EVs) on hepatocytes remains unclear. Using a rat model of partial hepatectomy (PH), this study found that bile EVs released 12 hours after PH significantly promoted hepatocyte proliferation. Transcriptome analysis revealed an upregulation of genes promoting cell cycle progression in hepatocytes treated with PH24-EVs.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Oncology
Takayuki Tokunaga, Masakuni Tateyama, Yasuteru Kondo, Satoshi Miuma, Shiho Miyase, Kentaro Tanaka, Satoshi Narahara, Hiroki Inada, Sotaro Kurano, Yoko Yoshimaru, Katsuya Nagaoka, Takehisa Watanabe, Hiroko Setoyama, Kotaro Fukubayashi, Motohiko Tanaka, Yasuhito Tanaka
Summary: This study aimed to evaluate the impact of therapeutic modifications of atezolizumab plus bevacizumab therapy in patients with unresectable hepatocellular carcinoma (uHCC). Patients with therapeutic modifications other than discontinuation of both drugs had favorable overall survival and time to progression, while those with discontinuation of both drugs had unfavorable outcomes. Modified liver function and immune-related adverse events increased the risk of discontinuation without other therapeutic modifications. Avoiding discontinuation without other therapeutic modifications may be the optimal management for uHCC.
Article
Oncology
Masatoshi Kudo, Kazuomi Ueshima, Issei Saeki, Toru Ishikawa, Yoshitaka Inaba, Naoki Morimoto, Hiroshi Aikata, Nobukazu Tanabe, Yoshiyuki Wada, Yasuteru Kondo, Masahiro Tsuda, Kazuhiko Nakao, Takanori Ito, Tetsuya Hosaka, Yusuke Kawamura, Teiji Kuzuya, Shunsuke Nojiri, Chikara Ogawa, Hironori Koga, Keisuke Hino, Masafumi Ikeda, Michihisa Moriguchi, Takashi Hisai, Kenichi Yoshimura, Junji Furuse, Yasuaki Arai
Summary: The TACTICS-L trial investigated the efficacy and safety of TACE plus lenvatinib in patients with unresectable intermediate-stage HCC. The results showed that LEN-TACE has significant anti-tumor activity and an acceptable safety profile.
Article
Medicine, General & Internal
Kosuke Sato, Jun Inoue, Takehiro Akahane, Tomoo Kobayashi, Satoshi Takai, Takuya Nakamura, Toshihiro Sato, Osamu Kimura, Masashi Ninomiya, Tomoaki Iwata, Akitoshi Sano, Mio Tsuruoka, Masazumi Onuki, Satoko Sawahashi, Hirofumi Niitsuma, Atsushi Masamune
Summary: Switching from ETV to TAF may effectively suppress HBV DNA levels in chronic hepatitis B patients with detectable HBV DNA, even at a low level.
TOHOKU JOURNAL OF EXPERIMENTAL MEDICINE
(2022)