Article
Cell Biology
Claudia Tanja Mierke, Alexander Hayn, Tony Fischer
Summary: Cell migration plays a critical role in physiological and pathological processes. The mechano-phenotype of the cytoskeleton affects the efficiency of cell migration and invasion. PINCH1, a focal adhesion protein, plays a key role in cell mechanics and facilitates 3D motility.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Review
Immunology
Changlin Du, Na Cai, Jiahui Dong, Chuanting Xu, Qi Wang, Zhenming Zhang, Jun Li, Cheng Huang, Taotao Ma
Summary: Neutrophils, a type of lymphocyte, undergo a unique form of cell death called NETosis, during which they release neutrophil extracellular traps (NETs) composed of modified chromatin structures and proteins. Recent research focuses on understanding the mechanisms of NET formation and the role of the cytoskeleton in this process. The enhanced understanding of the relationship between the cytoskeleton and NET formation will drive future research and the development of targeted therapies.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2023)
Article
Cell Biology
Jaekwang Jeong, Jae Hun Shin, Wenxue Li, Jun Young Hong, Jaechul Lim, Jae Yeon Hwang, Jean-Ju Chung, Qin Yan, Yansheng Liu, Jungmin Choi, John Wysolmerski
Summary: The lipid raft resident protein MAL2 contributes to the pathogenesis of malignancies, including breast cancer, through its effects on HER2 signaling and membrane stability. Physical interactions between HER2 and MAL2 in lipid rafts play a key role in maintaining low intracellular calcium concentrations and are enhanced in trastuzumab-resistant breast cancer cells, suggesting MAL2 as a potential therapeutic target.
Article
Biochemistry & Molecular Biology
Bianca Cruz Pachane, Ana Carolina Caetano Nunes, Thais Regiani Cataldi, Kelli Cristina Micocci, Bianca Caruso Moreira, Carlos Alberto Labate, Heloisa Sobreiro Selistre-de-Araujo, Wanessa Fernanda Altei
Summary: Hypoxia in triple-negative breast tumors promotes the secretion of extracellular vesicles, which enhance cell invasion. In this study, we investigated the invasive properties triggered by hypoxic small EVs derived from TNBC tumors. The results showed that hypoxic EVs have increased protein abundance and diversity, and are enriched in pro-invasive pathways.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biotechnology & Applied Microbiology
Hang Zhou, Naoto Isozaki, Kazuya Fujimoto, Ryuji Yokokawa
Summary: This study provides plausible explanations for the discrepancies in the regulation of MT flexural rigidity and develops a new method for evaluating MT rigidity. Furthermore, it reveals a new relationship between the dynamics and mechanics of MTs, where MT flexural rigidity decreases through three phases with increasing growth rate. The growth rate-dependent flexural rigidity of MTs influences pattern formation in collective motion, which is validated using machine learning.
JOURNAL OF NANOBIOTECHNOLOGY
(2021)
Article
Engineering, Biomedical
Yonggang Lv, Hongjun Wang, Gui Li, Boyuan Zhao
Summary: A study prepared 3D DECM scaffolds with suitable stiffness to mimic the tumor microenvironment of human breast tissue, exploring the impact of matrix stiffness on drug resistance in breast cancer cells.
BIOACTIVE MATERIALS
(2021)
Article
Nanoscience & Nanotechnology
Gerard Rubi-Sans, Agata Nyga, Elena Rebollo, Soledad Perez-Amodio, Jorge Otero, Daniel Navajas, Miguel A. Mateos-Timoneda, Elisabeth Engel
Summary: The study introduced a novel method to produce mesenchymal cell-derived matrices (CDMs) aimed at mimicking the fibrotic tumor microenvironment, showing that they closely mimic the composition, structure, and mechanical properties of human fibrotic ECM. CDMs have highly reproducible composition and tunable mechanical properties, and exhibit increased doxorubicin resistance in cancer cells following chemotherapeutic treatment in comparison to 3D culture in collagen hydrogels.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Chemistry, Multidisciplinary
Guotao Peng, Viviana Gonzalez, Ester Vazquez, Jon O. Lundberg, Bengt Fadeel
Summary: This study demonstrates that two-dimensional molybdenum disulfide (MoS2) nanosheets can trigger neutrophil extracellular traps (NETs) and possess antibacterial effects. Additionally, the MoS2 nanosheets exhibit nitrite reductase-like properties.
Article
Materials Science, Biomaterials
Swatilekha Hazra, Souradeep Dey, Biman B. Mandal, Charanya Ramachandran
Summary: Silk films have potential as substrates for culturing human corneal endothelial cells. Despite their higher tensile strength compared to native basement membrane, the expression of extracellular matrix proteins and integrins on silk films is comparable to native tissue, confirming their suitability for engineering corneal endothelial cells for transplantation.
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2023)
Article
Nanoscience & Nanotechnology
Zhuoyue Lei, Hongwei Jiang, Jie Liu, Yuping Liu, Di Wu, Chenwei Sun, Qijun Du, Liangwen Wang, Guohua Wu, Shuqi Wang, Xingdong Zhang
Summary: This study presents a novel method to enhance the biofabrication of cancer-derived extracellular vesicles (EVs) using audible acoustic wave (AAW), which significantly increases EV yield and promotes cancer cell migration and invasion. Additionally, AAW stimulation activates the ESCRT pathway and upregulates membrane fusion-associated proteins, contributing to the enhancement of EV biofabrication.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Biochemistry & Molecular Biology
Shunji Yunoki, Hirosuke Hatayama, Yoshimi Ohyabu, Kazuto Kobayashi
Summary: This study extracted collagen from barramundi fish scales and demonstrated its suitability for use in collagen fibril matrices (CFM). The fish scale collagen had high thermal stability and supported the well-developed growth of cells in 3D culture. This is the first report of fish CFM used in conventional 3D cell culture.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Cell Biology
Klaudia Sztolsztener, Karolina Konstantynowicz-Nowicka, Anna Pedzinska-Betiuk, Adrian Chabowski
Summary: This study investigated the effects of cannabigerol (CBG) on the composition of extracellular matrix (ECM) in fibrotic liver cells. The results showed that low concentrations of CBG reduced the expression of TGF-beta 1 and inhibited the deposition of collagen, suggesting that CBG may promote fibrosis regression and regeneration.
Article
Chemistry, Multidisciplinary
Linpeng Fan, Zengxiao Cai, Jian Zhao, Xungai Wang, Jing-Liang Li
Summary: This study reports a simple strategy for the scalable manufacturing of biomimetic materials. By interpenetrating natural polymers in an aqueous solution, a nanofibrous network with collagen fiber-like hierarchical structure and multilevel pores can be formed in situ. The resulting biomimetic matrices are bioactive and have tunable mechanical properties and hydrophilicity.
Article
Biochemistry & Molecular Biology
Eunae You, Jangho Jeong, Jieun Lee, Seula Keum, Ye Eun Hwang, Jee-Hye Choi, Sangmyung Rhee
Summary: Cell signals for growth factors are affected by the mechanical properties of the extracellular matrix (ECM). This study found that casein kinase 2 (CK2) plays an important role in the activation of α-tubulin acetyltransferase 1 (α-TAT1) induced by transforming growth factor (TGF)-β in a soft matrix. CK2 directly binds to the C-terminal domain of α-TAT1 and promotes the phosphorylation of serine at the 236th position, regulating its catalytic activity. These findings provide new insights into the molecular mechanisms underlying the activation of α-TAT1 in a soft matrix induced by TGF-β.
Article
Microbiology
Seo-Young Kim, Minjun Kim, Tae-Jong Kim
Summary: This study reveals a mechanism for regulating biofilm formation in Staphylococcus aureus through strain-specific physiological changes induced by a plant steroid called diosgenin. Diosgenin was found to either reduce or increase biofilm formation in different strains of S. aureus by affecting the expression of regulatory genes associated with physiological changes. The study provides insights into the signal transduction mechanisms that regulate biofilm formation in S. aureus based on physiological and gene expression changes.