Article
Oncology
Iori Motoo, Sohachi Nanjo, Takayuki Ando, Satoshi Yamashita, Toshikazu Ushijima, Ichiro Yasuda
Summary: The study reveals that in diffuse-type gastric cancers, ULK2 is methylated and knockdown of ULK2 can induce autophagy, cell migration, and EMT. mRNA microarray analysis showed that knockdown of ULK2 altered the expressions of oncogenic genes associated with cell migration and EMT.
Article
Medicine, Research & Experimental
Yuto Kubo, Koji Tanaka, Yasunori Masuike, Tsuyoshi Takahashi, Kotaro Yamashita, Tomoki Makino, Takuro Saito, Kazuyoshi Yamamoto, Tomoyuki Tsujimoto, Takashi Harino, Yukinori Kurokawa, Makoto Yamasaki, Kiyokazu Nakajima, Hidetoshi Eguchi, Yuichiro Doki
Summary: This study revealed that decreased mtDNA copy number induced EMT through modulation of MMP and DNA methylation in ESCC. Therapeutic strategies aimed at increasing mtDNA copy number and using DNMT inhibitors may be effective in preventing EMT and chemosensitivity resistance.
JOURNAL OF TRANSLATIONAL MEDICINE
(2022)
Review
Oncology
Cosima C. Hoch, Fabian Stoegbauer, Barbara Wollenberg
Summary: Salivary adenoid cystic carcinoma (SACC) is an aggressive tumor with a high tendency for metastasis. Epithelial-mesenchymal transition (EMT) plays a crucial role in the invasiveness of SACC by enhancing mobility and invasiveness. This manuscript reviews the latest research on potential biomarkers of EMT in SACC, aiming to identify new therapeutic targets and improve patient management.
Review
Biochemistry & Molecular Biology
Jian Qin, Jian Xu
Summary: Arginine methylation plays a critical role in regulating EMT, affecting signaling, transcription, and splicing regulation.
Article
Genetics & Heredity
Min Kyung Lee, Meredith S. Brown, Owen M. Wilkins, Diwakar R. Pattabiraman, Brock C. Christensen
Summary: The study indicates the importance of both distinct and shared epigenetic profiles associated with EMT processes, which may be targeted to prevent EMT progression.
Article
Oncology
Weijie Xue, Bingzi Dong, Yanjie Zhao, Yixiu Wang, Chenyu Yang, Yuwei Xie, Zhaojian Niu, Chengzhan Zhu
Summary: The study revealed that TTYH3 is highly expressed in CCA tissues at both mRNA and protein levels and its overexpression correlates with poor overall survival of the patients. TTYH3 promotes proliferation, migration, and invasion of CCA cells and induces epithelial-mesenchymal transition (EMT) through the Wnt/β-catenin pathway in CCA.
Article
Oncology
Hao Wu, Juanjuan Qiu, Zhenru Wu, Tao He, Chen Zhou, Qing Lv
Summary: This study reveals that ADCY6 is expressed at low levels in breast cancer, leading to increased proliferation, invasion, and migration of breast cancer cells. The low expression of ADCY6 is attributed to the low demethylation of TET1, and the methylation of ADCY6 can be altered by TET1. Bioinformatics analysis shows that TET1 is regulated by miR-27a-3p and regulates the methylation of ADCY6, thereby affecting the EMT process of breast cancer cells and promoting the malignant biological behavior of breast cancer.
FRONTIERS IN ONCOLOGY
(2022)
Article
Oncology
Lingge Yang, Zhiqiang Wu, Wei Sun, Peng Luo, Shiqi Chen, Yong Chen, Wangjun Yan, Yan Li, Chunmeng Wang
Summary: The study revealed that CCNDBP1 is a tumor suppressor gene and potential prognostic marker in DDL, with low expression being associated with poor prognosis in patients. CCNDBP1 inhibits invasion and metastasis of DDL cells by repressing EMT and promoting cell apoptosis. Methylation of specific DNA sites can decrease CCNDBP1 expression and worsen patient prognosis.
FRONTIERS IN ONCOLOGY
(2021)
Article
Cell Biology
Huafu Li, Chunming Wang, Linxiang Lan, Axel Behrens, Mona Tomaschko, Josue Ruiz, Qiao Su, Guangying Zhao, Cheng Yuan, Xing Xiao, Bo Li, Leping Yan, Wang Wu, Wuguo Li, Junzong Chen, Yulong He, Changhua Zhang
Summary: EMT plays a crucial role in the aggressiveness and metastasis of gastric cancer, especially in the infiltration of NK cells into tumor cells. Vinculin expression is closely related to cancer aggressiveness and distant metastasis, affecting patient survival prognosis. Additionally, VCL can influence EMT and tumor immunity by regulating EPCAM gene expression.
Review
Veterinary Sciences
Federico Armando, Federico Mazzola, Luca Ferrari, Attilio Corradi
Summary: This review discusses the general concept of epithelial-mesenchymal transition (EMT) and its regulation by various factors. It covers the role of transcription factors, microRNAs, reactive oxygen species, exosomes, microvesicles, and viruses in EMT. The review also examines EMT in embryonic development, fibrosis, and cancer metastasis, including discussions on the tumor microenvironment, pre-metastatic niches, and cancer stem cells. Additionally, it explores the use of EMT as a prognostic marker and provides insights into mesenchymal-epithelial transition (MET) in sarcomas. Therapeutic approaches for targeting EMT and MET are also addressed.
VETERINARY SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Yuyang Hong, Leiqin Liu, Yan Feng, Zhiqiang Zhang, Rui Hou, Qiong Xu, Jiantao Shi
Summary: mHapBrowser is a comprehensive database for visualizing and analyzing DNA methylation haplotypes, providing various functionalities and tools for studying gene expression and classifying tumors based on methylation patterns.
NUCLEIC ACIDS RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Maria Urbanova, Verona Buocikova, Lenka Trnkova, Sabina Strapcova, Viera Horvathova Kajabova, Emma Barreto Melian, Maria Novisedlakova, Miroslav Tomas, Peter Dubovan, Julie Earl, Jozef Bizik, Eliska Svastova, Sona Ciernikova, Bozena Smolkova
Summary: Pancreatic ductal adenocarcinoma (PDAC) is characterized by severe hypoxia, and DNA methylation plays an important role in PDAC aggressiveness and epithelial-to-mesenchymal transition (EMT). Inhibiting DNA methylation can reactivate silenced genes and potentially be targeted for PDAC progression.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Jia Chen, Shu-Fen Hou, Feng-Jie Tang, Dai-Song Liu, Zi-Zi Chen, Hong-Lian Zhang, Shao-Hua Wang
Summary: HOTAIR, through up-regulating Sp1 and targeting miR-199a, promotes stemness and progression of CSCC. Targeting HOTAIR, Sp1 or the underlying mechanisms may benefit CSCC treatment, as shown by functional assays and in vivo models.
Review
Oncology
Fernan Gomez-Valenzuela, Enrico Escobar, Ricardo Perez-Tomas, Viviana P. Montecinos
Summary: COX-2 overexpression in the tumor microenvironment can promote epithelial-mesenchymal transition (EMT) and drive initial metastatic-related events.
FRONTIERS IN ONCOLOGY
(2021)
Review
Plant Sciences
Kajal Samantara, Aalok Shiv, Lorenna Lopes de Sousa, Karansher Singh Sandhu, Parichita Priyadarshini, Sourav Ranjan Mohapatra
Summary: Epigenetics is an important mechanism that can help plants cope with various stressors by altering gene expression without involving changes in DNA sequences. It provides diversity to aid plants in responding to the impacts of climate change.
ENVIRONMENTAL AND EXPERIMENTAL BOTANY
(2021)
Review
Oncology
Veronica Davalos, Manel Esteller
Summary: Cancer development is caused by changes in the structure and function of the genome. Epigenetic alterations regulate gene expression programs that promote tumorigenesis and can be used as useful tools in clinical decision making. Unlike genetic changes, epigenetic modifications can be reversed, making them an attractive target for drug development.
CA-A CANCER JOURNAL FOR CLINICIANS
(2023)
Review
Oncology
Iris van der Strate, Fatemeh Kazemzadeh, Iris D. Nagtegaal, Debbie Robbrecht, Agnes van de Wouw, Catarina S. Padilla, Saskia Duijts, Manel Esteller, F. Anthony Greco, Nicholas Pavlidis, Amir Qaseem, Petur Snaebjornsson, Sophie Veldhuijzen van Zanten, Caroline Loef
Summary: The study proposes a multi-layer classification for the diagnostic workup of CUP using the Delphi method. The recommended initial diagnostic includes history and physical examination, full blood count, serum marker analysis, biopsy of accessible lesion, CT scan, and immunohistochemical testing. Consensus was also reached on the need for an ideal diagnostic lead time for CUP patients.
CRITICAL REVIEWS IN ONCOLOGY HEMATOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Paolo Petazzi, Olga Caridad Jorge-Torres, Antonio Gomez, Iolanda Scognamiglio, Jordi Serra-Musach, Angelika Merkel, Daniela Grases, Clara Xiol, Mar O'Callaghan, Judith Armstrong, Manel Esteller, Sonia Guil
Summary: Rett syndrome is a severe neurodevelopmental disease caused by mutations in the MeCP2 gene, which affects synaptic plasticity and neuronal activity. The MeCP2 protein is critical for the proper expression of immediate-early genes (IEGs) involved in activity-dependent responses, and abnormal expression of IEGs is found in MeCP2-deficient neurons and in RTT patients. These findings highlight the importance of IEGs in synaptic development and the regulatory role of MeCP2.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
David Ortega-Alarcon, Rafael Claveria-Gimeno, Sonia Vega, Olga C. Jorge-Torres, Manel Esteller, Olga Abian, Adrian Velazquez-Campoy
Summary: Hydroxymethylated cytosine (5hmC) is a stable DNA epigenetic mark that interacts with MeCP2 in a distinct mode with a specific thermodynamic signature. Mutations associated with Rett syndrome alter the interaction between MeCP2 and dsDNA in a cytosine modification-specific manner, which may be correlated with disease onset time and clinical severity score.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Biochemistry & Molecular Biology
Miriam Gene, Miriam Cuatrecasas, Irene Amat, Jesus Alberto Veiga, Maria Jesus Fernandez Acenero, Victoria Fuste Chimisana, Jordi Tarragona, Ismael Jurado, Rebeca Fernandez-Victoria, Carolina Martinez Ciarpaglini, Cristina Alenda Gonzalez, Carlos Zac, Pilar Ortega de la Obra, Maria Teresa Fernandez-Figueras, Manel Esteller, Eva Musulen
Summary: Colitis-associated colorectal carcinoma (CAC) in inflammatory bowel disease (IBD) is caused by p53 alterations and follows a chronic inflammation-dysplasia-cancer carcinogenesis pathway. Gastric metaplasia (GM) is described as the initial event of serrated colorectal cancer (CRC) in the colon mucosa under chronic stress. This study analyzed p53 alterations and microsatellite instability (MSI) to characterize CAC and investigate its relationship with GM. Results showed that p53 mutation pattern was present in more than half of the CAC cases, with stable (MSS) tumors being the most common and MUC5AC negative. Only six unstable (MSI-H) tumors had wild-type p53 pattern (p=0.010) and were MUC5AC positive (p=0.005). MUC5AC staining was more frequently observed in intestinal mucosa with inflammation or chronic changes than in CAC, particularly in those with wild-type p53 pattern and MSS.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Cankut Cubuk, Carlos Loucera, Maria Pena-Chilet, Joaquin Dopazo
Summary: Reprogramming metabolism is a key feature of cancer, and recent research suggests that metabolites may play a crucial role in regulating signaling pathways. Mechanistic models were used to analyze the metabolic and signaling pathway activities of Breast invasive Carcinoma (BRCA), and Gaussian Processes combined with SHapley Additive exPlanations (SHAP) were employed to identify potential causal relationships between metabolite production and signaling pathway regulation. A total of 317 metabolites were found to significantly impact signaling circuits, revealing a complex crosstalk between signaling and metabolic pathways.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Oncology
Ruben Olivera-Salguero, Elia Segui, Juan Miguel Cejalvo, Mafalda Oliveira, Pablo Tolosa, Maria Vidal, Marcos Malumbres, Joaquin Gavila, Cristina Saura, Sonia Pernas, Rafael Lopez, Mireia Margeli, Judith Balmana, Montserrat Munoz, Isabel Blancas, Valentina Boni, Eva Ciruelos, Elena Galve, Antonia Perello, Rodrigo Sanchez-Bayona, Susana de la Cruz, Miguel de la Hoya, Patricia Galvan, Esther Sanfeliu, Blanca Gonzalez-Farre, Valeria Sirenko, Aura Blanch-Torras, Jordi Canes, Helena Masanas, Rosa Olmos, Margarita Forns, Aleix Prat, Ana Casas, Tomas Pascual
Summary: This study aims to promote active patient participation in the management of metastatic breast cancer (mBC) through a digital tool, providing NGS testing and medical interpretation and recommendations from a multidisciplinary molecular advisory board (MAB), to overcome the challenges of limited access and high cost of NGS in clinical practice.
FRONTIERS IN ONCOLOGY
(2023)
Article
Cell Biology
Juan J. Alburquerque-Bejar, Pablo Navajas-Chocarro, Maria Saigi, Ana Ferrero-Andres, Juan M. Morillas, Andrea Vilarrubi, Antonio Gomez, JoseL. Mate, Ana M. Munoz-Marmol, Octavio A. Romero, Pedro Blecua, Veronica Davalos, Manel Esteller, Eva Pros, Paula Llabata, Manuel Torres-Diz, Anna Esteve-Codina, Montse Sanchez-Cespedes
Summary: This study reveals subsets of lung cancer that are completely refractory or show submaximal response to interferon-γ (IFNy) stimulation, which may be due to JAK2 or IFNGR1 inactivation and impaired IFNy-stimulated genes (IySGs) expression. It also suggests that MYC activation downregulates JAK2 and impairs IySGs stimulation, potentially predicting response to anti-programmed death-1/ligand-1 (PD1/L1) therapies.
CELL REPORTS MEDICINE
(2023)
Review
Oncology
Maxime Janin, Veronica Davalos, Manel Esteller
Summary: Most cancer-related deaths and illnesses can be attributed to metastasis. The role of epigenetic and epitranscriptomic changes in the origin and progression of cancer has been extensively demonstrated. These two-layer regulatory mechanisms, driven by DNA or RNA modifiers, are finely controlled in normal tissue but dysregulated in cancer. Understanding these mechanisms could have important clinical implications for prevention and management of advanced malignancies. Reversing these epi-alterations with small molecules or inhibitors against epi-modifiers could offer novel therapeutic alternatives.
CANCER AND METASTASIS REVIEWS
(2023)
Article
Biochemistry & Molecular Biology
Miriam Paya-Milans, Maria Pena-Chilet, Carlos Loucera, Marina Esteban-Medina, Joaquin Dopazo
Summary: Soft tissue sarcoma is a group of rare and difficult-to-treat cancers, and the study shows that computational analysis of gene expression data can reveal the importance of signaling pathways in patient survival. Thirteen signaling circuits were identified as predictors of patient survival, and the protective role of the immune system in the tumor microenvironment and potential therapeutic strategies were also described.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Letter
Oncology
Carlos A. Garcia-Prieto, Veronica Davalos, Manel Esteller
JNCI-JOURNAL OF THE NATIONAL CANCER INSTITUTE
(2023)
Article
Oncology
Lucia Salz, Alexander Seitz, Daniel Schaefer, Julia Franzen, Tatjana Holzer, Carlos A. Garcia-Prieto, Iris Buerger, Olaf Hardt, Manel Esteller, Wolfgang Wagner
Summary: CAR T cell expansion during culture leads to DNA hypermethylation, which downregulates the expression of genes relevant to T cell function. This hypermethylation signature can predict cell culture time and is associated with reduced long-term survival and therapeutic outcome in CAR T cell products.
Article
Biochemistry & Molecular Biology
Javier Perez-Florido, Carlos S. Casimiro-Soriguer, Francisco Ortuno, Jose L. Fernandez-Rueda, Andrea Aguado, Maria Lara, Cristina Riazzo, Manuel A. Rodriguez-Iglesias, Pedro Camacho-Martinez, Laura Merino-Diaz, Inmaculada Pupo-Ledo, Adolfo de Salazar, Laura Vinuela, Ana Fuentes, Natalia Chueca, Federico Garcia, Joaquin A. Dopazo, Jose Lepe
Summary: Recombination is an evolutionary strategy to acquire new viral properties quickly. The Andalusian genomic surveillance strategy has revealed a high number of co-infections, providing an ideal setting for the emergence of new recombinants. Whole genome sequencing of SARS-CoV-2 has been conducted, detecting three novel recombinant variants with two break points. The increased frequency of co-infection and recombination raises the risk of recombinant variants with increased transmissibility and pathogenicity.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)