Article
Oncology
Meiou Dai, Julien Boudreault, Ni Wang, Sophie Poulet, Girija Daliah, Gang Yan, Alaa Moamer, Sergio A. Burgos, Siham Sabri, Suhad Ali, Jean-Jacques Lebrun
Summary: The study demonstrates distinct and novel functions of CDK4 and CDK6 in regulating tumor formation and metastatic colonization in various solid tumors. Transcriptomic analysis reveals these kinases to regulate cancer progression through different mechanisms, with CDK4 affecting prometastatic inflammatory signaling and CDK6 controlling DNA replication and repair processes. Inhibition of CDK6 but not CDK4 results in defective DNA repair and increased DNA damage, suggesting potential targeted therapy for deadly tumors.
Article
Cell Biology
Kwan Long Mung, William B. Eccleshall, Niina M. Santio, Adolfo Rivero-Muller, Paivi J. Koskinen
Summary: The study demonstrates that PIM kinases negatively regulate LKB1 and affect AMPK activity in an LKB1-dependent manner. Cells lacking both LKB1 and PIMs exhibit impaired cell proliferation and tumor growth in the context of cancer, suggesting a shared signaling role of these kinases.
CELL COMMUNICATION AND SIGNALING
(2021)
Article
Biochemistry & Molecular Biology
Yun Ma, Guijie Guo, Tingting Li, Faxin Wen, Jianling Yang, Biao Chen, Xuefei Wang, Ji-Long Chen
Summary: The study reveals that lncRNA-IUR1 plays an inhibitory role in Abl-mediated cellular transformation, providing new insights into the molecular mechanisms underlying Abl-induced leukemogenesis.
Article
Biochemistry & Molecular Biology
Kwan Long Mung, Annika Meinander, Paivi J. Koskinen
Summary: LDHA is an important enzyme in glycolysis, and it can be phosphorylated by PIM kinases at serine 161. Phosphorylation suppresses the degradation of nuclear LDHA and promotes its interaction with 14-3-3 proteins. PIM kinases support the expression and function of nuclear LDHA through phosphorylation-dependent interactions.
Article
Cell Biology
Rachel K. Toth, Regina Solomon, Noel A. Warfel
Summary: The study revealed that PIM kinases are upregulated at the protein level in hypoxia independently of HIF-1, and the deubiquitinase USP28 plays a key role in stabilizing PIM1 and PIM2 by reducing their ubiquitination levels in hypoxic conditions. Interaction between PIM kinases and USP28 increased in response to hypoxia, leading to enhanced protein stability and decreased ubiquitination. USP28 knockdown slowed tumor growth and reduced PIM1 levels in vivo, highlighting its role as an important regulator of the hypoxia-induced protumorigenic signaling pathway.
Article
Microbiology
Bin Wang, Xiaoying Zhou, Scott A. Gerber, Jennifer J. Loros, Jay C. Dunlap
Summary: The study reveals that changes in intracellular calcium levels in the Neurospora clock affect the phosphorylation of core clock components, leading to a shortened circadian period length.
Article
Multidisciplinary Sciences
Masahiro Shimizu, Hiroshi Shibuya, Nobuyuki Tanaka
Summary: Cancer stem cells (CSCs) have the ability to initiate and regenerate tumors. This study reveals that the oncogenic RAS gene can induce the expression of reprogramming factor SOX2, leading to the generation of CSCs. The activation of CDK1 and enhancement of protein O-GlcNAcylation are crucial for SOX2 induction and CSC generation.
SCIENTIFIC REPORTS
(2022)
Article
Cell Biology
Chen Wang, Jie Zeng, Li-Jie Li, Min Xue, Si-Li He
Summary: The study showed that Cdc25A regulates ferroptosis in cervical cancer cells by modulating the levels of PKM2 and ErbB2, affecting the response of cervical cancer cells to sorafenib treatment.
CELL DEATH & DISEASE
(2021)
Article
Biochemistry & Molecular Biology
Lan Li, Bin Li, Sirui Zhu, Long Wang, Limei Song, Jia Chen, Zhenhua Ming, Xuanming Liu, Xiushan Li, Feng Yu
Summary: In this study, a previously unknown mechanism was identified where a plasma membrane protein kinase positively regulates ABI2. Further analysis revealed that TMK4 phosphorylates ABI2 to enhance its activity in the ABA signaling pathway. The phosphorylation of ABI2 by TMK4 was shown to complement the ABA hypersensitive phenotype.
JOURNAL OF INTEGRATIVE PLANT BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Victoria R. Mingione, Zachariah H. Foda, YiTing Paung, Hannah Philipose, Aziz M. Rangwala, Yibing Shan, Markus A. Seeliger
Summary: Allostery plays a critical role in regulating protein activity and has important implications in human disease and drug discovery. However, identifying allosteric binding sites remains challenging due to their cryptic nature and lack of clear geometric or chemical features. In this study, we present a computational protocol that utilizes unbiased ligand binding simulations to predict allosteric ligand binding sites. We demonstrate the feasibility of this protocol using Src kinase as a model system and validate a novel allosteric site using virtual screening and experimental assays. This study provides proof-of-concept for the use of unbiased ligand binding simulations in identifying cryptic allosteric binding sites.
JOURNAL OF MOLECULAR BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Fiorella Faienza, Federica Polverino, Girish Rajendraprasad, Giacomo Milletti, Zehan Hu, Barbara Colella, Deborah Gargano, Flavie Strappazzon, Salvatore Rizza, Mette Vixo Vistesen, Yonglun Luo, Manuela Antonioli, Valentina Cianfanelli, Caterina Ferraina, Gian Maria Fimia, Giuseppe Filomeni, Daniela De Zio, Joern Dengjel, Marin Barisic, Giulia Guarguaglini, Sabrina Di Bartolomeo, Francesco Cecconi
Summary: AMBRA1 is a key factor for nervous system development, primarily associated with autophagy and cell proliferation control. This study reveals that AMBRA1 is phosphorylated during mitosis and is critical for spindle function and orientation, driven by NUMA1 protein. The localization and dynamics of NUMA1 are dependent on AMBRA1 presence, phosphorylation, and binding ability. These findings suggest an additional role of AMBRA1 in tissue morphogenesis and differentiation, which could have implications for development and cancer oncogenesis.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2023)
Article
Microbiology
Lixiang Zhai, Chunhua Huang, Ziwan Ning, Yijing Zhang, Min Zhuang, Wei Yang, Xiaolei Wang, Jingjing Wang, Lu Zhang, Haitao Xiao, Ling Zhao, Pallavi Asthana, Yan Y. Lam, Chi Fung Willis Chow, Jian-dong Huang, Shuofeng Yuan, Kui Ming Chan, Chun-Su Yuan, Johnson Yiu-Nam Lau, Hoi Leong Xavier Wong, Zhao-xiang Bian
Summary: Diarrhea-predominant irritable bowel syndrome (IBS-D) is a common functional gastrointestinal disorder associated with increased gut motility caused by elevated serotonin levels. This study found that the bacterium Ruminococcus gnavus has a pathogenic role in IBS-D by stimulating serotonin production. R. gnavus catabolizes dietary phenylalanine and tryptophan to generate phenethylamine and tryptamine, which directly stimulate serotonin synthesis in intestinal cells. This increase in serotonin levels induced by R. gnavus promotes gut motility, but can be inhibited by blocking a specific receptor. These findings provide insights into the molecular and pathogenetic mechanisms of how gut microbial metabolites derived from essential amino acids regulate gut motility.
CELL HOST & MICROBE
(2023)
Article
Chemistry, Multidisciplinary
Lucia Tapia, Naiara Solozabal, Jordi Sola, Yolanda Perez, W. Todd Miller, Ignacio Alfonso
Summary: The study introduces a new approach to selectively protect tyrosine residues in peptides from phosphorylation by the Src TK enzyme using pseudopeptidic cages. Fluorescence emission titrations indicate that the most efficient cage inhibitors strongly bind peptide substrates, with a good correlation between binding constant and inhibitory potency. Further experimental evidence supports the proposed mechanism of selectively protecting substrates through supramolecular interactions, showcasing the potential of supramolecular complexes for modulating TK signaling.
CHEMISTRY-A EUROPEAN JOURNAL
(2021)
Article
Biochemistry & Molecular Biology
Trisiani Affandi, Angela M. Ohm, Dany Gaillard, Ami Haas, Mary E. Reyland
Summary: The study demonstrates that tyrosine kinase inhibitors (TKIs) provide radioprotection to salivary gland tissues by enhancing DNA repair mechanisms. TKIs increase the repair of DNA double-stranded breaks, regulate pathways involved in DNA repair, and prime cells for more efficient repair post-irradiation, suggesting a potential clinical application in head and neck cancer patients receiving radiation therapy.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Correction
Biochemistry & Molecular Biology
Xu Zhang, Zunnan Huang, Yeye Guo, Ta Xiao, Ling Tang, Shuang Zhao, Lisha Wu, Juan Su, Weiqi Zeng, Hongbin Huang, Zheng Li, Juan Tao, Jianda Zhou, Xiang Chen, Cong Peng
Summary: A correction to this paper has been published, for detailed information please visit the provided link.
Article
Cell Biology
Ruijie Zhang, Chunxiao Liu, Liu Yang, Tong Ji, Nana Zhang, Xiaoqing Dong, Xin Chen, Jing Ma, Wei Gao, Shile Huang, Long Chen
Summary: Research shows that PD toxins induce neuronal apoptosis by generating H2O2 through NOX2, hindering the AMPK/Akt-mTOR signaling pathway. Inhibiting NOX2 or using antioxidants may help alleviate neuronal loss caused by oxidative stress.
CELLULAR SIGNALLING
(2022)
Article
Integrative & Complementary Medicine
Yu Gan, Shi-Le Huang, Meng-Qi Luo, Min Chen, Hui Zheng
Summary: The efficacy of acupuncture combined with usual care in treating irritable bowel syndrome (IBS) has been studied through network meta-analysis. The results show that manual acupuncture combined with usual care is the most effective approach, and adding manual acupuncture to usual care provides additional benefits.
ACUPUNCTURE IN MEDICINE
(2022)
Review
Pharmacology & Pharmacy
Di Qin, Qing-Feng Tao, Shi-Le Huang, Min Chen, Hui Zheng
Summary: This study compared the efficacy of eluxadoline and antispasmodics in the treatment of irritable bowel syndrome (IBS). The results showed that eluxadoline was as effective as antispasmodics in relieving abdominal pain, but had more reported adverse events. Therefore, antispasmodics remain the first choice for the treatment of IBS.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Pharmacology & Pharmacy
Qinghui Lan, Peiqing Guan, Chunzheng Huang, Shile Huang, Peiling Zhou, Changzheng Zhang
Summary: This study demonstrated that arecoline significantly increased the firing rate of dopaminergic neurons and induced burst firing. This may contribute to areca nut dependency and provide a potential target for cessation therapy.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Immunology
Tithi Roy, Sergette Banang-Mbeumi, Samuel T. Boateng, Emmanuelle M. Ruiz, Roxane-Cherille N. Chamcheu, Lin Kang, Judy A. King, Anthony L. Walker, Bolni Marius Nagalo, Konstantin G. Kousoulas, Stephane Esnault, Shile Huang, Jean Christopher Chamcheu
Summary: Fisetin has potential therapeutic effects on refractory psoriasis by inhibiting abnormal immune response, promoting keratinocyte differentiation and autophagy, and reducing disease inflammation.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Yanwei Zhang, Xiaojuan Chi, Jingyun Hu, Shulin Wang, Senhong Zhao, Yanan Mao, Benqun Peng, Jilong Chen, Song Wang
Summary: Studies have shown that the long noncoding RNA LINC02574 is induced by influenza A virus (IAV) infection and plays a crucial role in inhibiting IAV replication. The expression of LINC02574 is stimulated by viral genomic RNA, poly (I:C), or interferons (IFNs) through the RIG-I-dependent interferon signaling pathway. Knockdown of LINC02574 impairs the expression of type I and type III IFNs, multiple ISGs, and the activation of STAT1 triggered by IAV infection, indicating that LINC02574 positively regulates the innate immune response.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Correction
Neurosciences
Qianyun Yu, Ruijie Zhang, Tianjing Li, Liu Yang, Zhihan Zhou, Long Hou, Wen Wu, Rui Zhao, Xiaoling Chen, Yajie Yao, Shile Huang, Long Chen
MOLECULAR NEUROBIOLOGY
(2023)
Article
Neurosciences
Qianyun Yu, Ruijie Zhang, Tianjing Li, Liu Yang, Zhihan Zhou, Long Hou, Wen Wu, Rui Zhao, Xiaoling Chen, Yajie Yao, Shile Huang, Long Chen
Summary: Defective autophagy is involved in the development of Parkinson's disease (PD), and PD toxins impede autophagy through the PTEN/Akt signaling pathway, leading to neuronal apoptosis. Activation of PTEN and inactivation of Akt by PD toxins inhibit autophagy, and this process is regulated by the protein Parkin. Manipulation of PTEN/Akt/autophagy signaling with antioxidants could be a potential therapeutic strategy for preventing neuronal loss in PD.
MOLECULAR NEUROBIOLOGY
(2023)
Article
Biology
Terry C. Lairmore, Jehan Abdulsattar, Arrigo De Benedetti, Runhua Shi, Shile Huang, Md Imtiaz Khalil, Stephan N. Witt
Summary: Immunohistochemical analysis showed an inverse association between menin expression and tumor grade in cholangiocarcinoma. Menin expression was higher in low-grade tumors and lower in high-grade tumors. Menin expression was also positively associated with fibrosis status.
BMC RESEARCH NOTES
(2023)
Article
Biochemistry & Molecular Biology
Boyue Zhang, Ting Cai, Hongling He, Xuezhe Huang, Guie Chen, Yanqin Lai, Yongwen Luo, Shile Huang, Jun Luo, Xiaofeng Guo
Summary: Rabies is a highly fatal zoonotic disease with no effective treatment available. Recent studies have shown that the tripartite-motif protein (TRIM) family plays a role in regulating viral replication. Specifically, tripartite-motif protein 21 (TRIM21) has been found to be upregulated in the brain tissue of mice infected with rabies virus and its knockdown inhibits viral replication. Knockdown of interferon-alpha and interferon-beta further modulates the inhibition of viral replication caused by TRIM21 knockdown. TRIM21 regulates RABV replication via the IRF7-IFN axis, highlighting its potential as a target for rabies treatment or management.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Virology
Mohamed Maarouf, Lulu Wang, Yiming Wang, Kul Raj Rai, Yuhai Chen, Min Fang, Ji-Long Chen
Summary: circRNAs are abundant and highly stable non-coding RNAs in eukaryotic cells, and they play critical roles in regulating viral infection and replication. They can regulate gene expression through microRNA and protein sponging, as well as encoding small polypeptides. Due to their stability and low immunogenicity, circRNAs also have potential applications in diagnosis, prognosis, RNA vaccines, and antiviral therapy.
Article
Cell Biology
Shasha Liu, Siya Liu, Ziding Yu, Wenzhuo Zhou, Meichun Zheng, Rongrong Gu, Jinxuan Hong, Zhou Yang, Xiaojuan Chi, Guijie Guo, Xinxin Li, Na Chen, Shile Huang, Song Wang, Ji-Long Chen
Summary: This study reveals that early-stage infection with various viruses, including influenza A virus, leads to IL-6-independent phosphorylation of STAT3 Y705, which is critical for antiviral immunity. Knockin mice with STAT3Y705F/1 mutation show suppressed antiviral response, lung tissue injury, and poor survival upon IAV infection. The phosphorylation of STAT3 Y705 restrains IAV pathogenesis by repressing excessive IFN production, and blocking the phosphorylation enhances IFN expression and potentiates IAV virulence in mice. Knockout of IFNAR1 or IFNLR1 in STAT3Y705F/1 mice protects them from lung injury and reduces viral load.
Article
Genetics & Heredity
Boyue Zhang, Ting Cai, Hongling He, Xuezhe Huang, Yongwen Luo, Shile Huang, Jun Luo, Xiaofeng Guo
Summary: TRIM25 plays a crucial role in the infection of rabies virus, regulating its replication through the RIG-I-IFN axis. This finding suggests that TRIM25 may serve as a potential target for the development of antiviral drugs against rabies.
Meeting Abstract
Oncology
Lin Li, Lie Liu, Shile Huang
Meeting Abstract
Oncology
Shile Huang, Lei Liu, Yan Luo