Article
Microbiology
Jieyi Li, Zhongwang Zhang, Jianliang Lv, Zhongyuan Ma, Li Pan, Yongguang Zhang
Summary: This study conducted a comprehensive analysis of the phosphoproteome of Senecavirus A (SVA) infection, revealing the involvement of phosphorylated proteins in apoptosis and spliceosome pathways, with a majority located in the nucleus. Additionally, several proteins related to SVA infection were identified, and the reversal of kinase activity following SVA infection was predicted. This is the first phosphoproteomics analysis of SVA infection and expands our understanding of the virus's interactions with host cells.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Medicine, Research & Experimental
Nathalia D. de Moura Sperotto, Rodrigo Braccini Madeira Silva, Marcia Alberton Perello, Ana Flavia Borsoi, Adilio da Silva Dadda, Candida Deves Roth, Raquel Dal Sasso Freitas, Ana Paula Duarte de Souza, Deise do Nascimento de Freitas, Jaqueline Nascimento Picada, Jayne Torres de Sousa, Debora Dreher Nabinger, Stefani Altenhofen, Carla Denise Bonan, Valnes S. Rodrigues-Junior, Cristiano Valim Bizarro, Luiz Augusto Basso, Pablo Machado
Summary: The study investigated the effects of the hTP inhibitor CPBMF-223 in a xenograft tumor model induced by human colorectal carcinoma cells, showing significant inhibition of tumor growth, reduction in vessel number, and decrease in cell migration. Additionally, CPBMF-223 demonstrated a favorable toxicity profile and pharmacokinetic characteristics, highlighting its potential as a promising drug candidate for colorectal cancer.
BIOMEDICINE & PHARMACOTHERAPY
(2021)
Article
Multidisciplinary Sciences
Matthew F. Buas, Charles W. Drescher, Nicole Urban, Christopher I. Li, Lisa Bettcher, Nitai C. Hait, Kirsten B. Moysich, Kunle Odunsi, Daniel Raftery, Li Yan
Summary: The study found significant differences in lipid metabolism between ovarian cancer and benign disease, with certain lipid species showing significantly reduced concentrations in patients. Some lipids exhibited greater alterations in early- (such as diacylglycerols) or late-stage (such as lysophospholipids) cases, and multiple lipids in plasma and ascites were positively correlated.
SCIENTIFIC REPORTS
(2021)
Article
Biotechnology & Applied Microbiology
Pavel Sinitcyn, Alicia L. Richards, Robert J. Weatheritt, Dain R. Brademan, Harald Marx, Evgenia Shishkova, Jesse G. Meyer, Alexander S. Hebert, Michael S. Westphall, Benjamin J. Blencowe, Juergen Cox, Joshua J. Coon
Summary: Deep proteome sequencing provides an 80% coverage of the human proteome. Shotgun proteomics experiments detect about 10,000 human proteins from a single sample, but fail to distinguish protein variants and isoforms. Our study using various methods identifies a million unique peptides and provides evidence for the translation of nonsynonymous variants.
NATURE BIOTECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Haojie Li, Hong Chen, Xiangzheng Zhang, Yaling Qi, Bing Wang, Yiqiang Cui, Jie Ren, Yichen Zhao, Yu Chen, Tianyu Zhu, Yue Wang, Liping Yao, Yueshuai Guo, Hui Zhu, Yan Li, Chenghao Situ, Xuejiang Guo
Summary: Using phosphoproteomic approach, this study identified the phosphorylation sites and regulatory factors essential for meiosis progression and male fertility. CDK9 was found to be important for meiosis progression to metaphase I, while HASPIN played a critical role in male fertility. These findings highlight the importance of protein phosphorylation in the regulation of meiosis.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Olayiwola O. Oduwole, Ariel Poliandri, Anthony Okolo, Phil Rawson, Milena Doroszko, Marcin Chrusciel, Nafis A. Rahman, Gilberto Serrano de Almeida, Charlotte L. Bevan, Wolfgang Koechling, Ilpo T. Huhtaniemi
Summary: The study suggests that FSH, rather than LH, may directly stimulate the growth of androgen-independent prostate cancer. Continuous suppression of FSH during GnRH antagonist treatment may offer a therapeutic advantage over agonist in inhibiting tumor growth.
Article
Cell Biology
Jiajia Li, Siqi Wen, Biao Li, Na Li, Xianquan Zhan
Summary: The study identified 595 differentially phosphorylated proteins in human nonfunctional pituitary neuroendocrine tumors (NF-PitNETs), with involvement in multiple biological processes and signaling pathways. Kinase analysis of these phosphorylated proteins revealed potential new drug targets. Further validation through experiments confirmed the research findings.
Article
Hematology
Yuanliang Peng, Li Tang, Yanan Li, Jianhui Song, Hong Liu, Pan Wang, Zhizhou Zhong, Yifei Yang, Shihui Wang, Lixiang Chen, Ji Zhang, Shijie Zhang, Zi Wang, Min Li, Long Liang, Jing Liu
Summary: This study characterized the quantitative and qualitative analysis of phosphorylated proteins during human early erythropoiesis using tandem mass-tagging technology. The results revealed the important regulatory role of phosphorylated proteins in erythroid development and identified functional modules related to cell cycle and signaling pathways.
BRITISH JOURNAL OF HAEMATOLOGY
(2022)
Article
Endocrinology & Metabolism
Roni H. G. Wright, Viviana Vastolo, Javier Quilez Oliete, Jose Carbonell-Caballero, Miguel Beato
Summary: This study used antibody microarrays and phosphoproteomics to investigate the signaling pathways involved in breast cancer cell proliferation following progestin exposure. Through detailed analysis, new key proteins, phosphorylation sites, and signaling pathways were identified, shedding light on the mechanisms underlying cell cycle regulation in breast cancer cells. The study also revealed changes in protein complexes involved in nuclear structure and epithelial to mesenchyme transition. This research provides valuable insights for understanding breast cancer biology and may have implications for cancer management.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Cell Biology
Cong He, Luoyan Sheng, Deshen Pan, Shuai Jiang, Li Ding, Xiaojun Ma, Yaohua Liu, Deshui Jia
Summary: High-grade glioma, characterized by extensive tumor heterogeneity, was analyzed through single-cell RNA sequencing to identify cellular and molecular mechanisms driving tumor heterogeneity. The study revealed a dominance of tumor-associated macrophages in the immune microenvironment, along with five distinct subpopulations of tumor cells and highlighted the critical role of the SPP1/CD44 axis in glioma progression.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Oncology
Can Chen, Yimin Cai, Yizhuo Liu, Shuoni Chen, Yanmin Li, Fuwei Zhang, Ming Zhang, Zequn Lu, Pingting Ying, Jinyu Huang, Linyun Fan, Xiaomin Cai, Caibo Ning, Wenzhuo Wang, Yuan Jiang, Heng Zhang, Shuhui Yang, Zhihua Wang, Xiaoyang Wang, Shaokai Zhang, Chaoqun Huang, Bin Xu, Zhenming Fu, Qibin Song, Mingjuan Jin, Kun Chen, Hongda Chen, Min Dai, Xiaoping Miao, Xiaojun Yang, Ying Zhu, Jianbo Tian
Summary: This study systematically identified 38,660 mbQTLs across multiple cancer types, with a strong enrichment of mbQTLs among transcription factor binding sites and chromatin regulatory elements, as well as in cancer GWAS loci. This provides additional insights into cancer etiology.
Article
Public, Environmental & Occupational Health
Chen Liu, Jae Hyoung Lee, Amanda J. Gupta, Austin Tucker, Chris Larkin, Patricia Turimumahoro, Achilles Katamba, J. Lucian Davis, David Dowdy
Summary: Human-centred design (HCD) is a problem-solving approach used to develop global health interventions. The cost-effectiveness of HCD depends on the number of clients reached and the trade-off between HCD costs and intervention delivery costs.
Article
Biochemistry & Molecular Biology
Shu En Lim, Megan D. Joseph, Charlotte M. de Winde, Sophie E. Acton, Sabrina Simoncelli
Summary: Upon initial immune challenge, dendritic cells (DCs) migrate to lymph nodes and interact with fibroblastic reticular cells (FRCs) via C-type lectin-like receptor 2 (CLEC-2). CLEC-2 binds to the membrane glycoprotein podoplanin (PDPN) on FRCs, inhibiting actomyosin contractility through the FRC network and permitting lymph node expansion. CD44 is required for FRCs to respond to DCs and stabilize large pools of PDPN at the membrane of FRCs upon CLEC-2 interaction, facilitating cellular crosstalk between FRCs and DCs.
Article
Nanoscience & Nanotechnology
Jia Ouyang, Yu Jiang, Chao Deng, Zhiyuan Zhong, Qing Lan
Summary: The study demonstrated that doxorubicin hydrochloride loaded in ApoE peptide-functionalized reduction-sensitive polymersomes induces potent and safe chemotherapy of orthotopic U-87 MG model in nude mice, effectively inhibiting glioblastoma invasion with minimal side effects.
INTERNATIONAL JOURNAL OF NANOMEDICINE
(2021)
Article
Biochemical Research Methods
She Chen, Yue Zhao, Lin Li, Xinhui Wang, Sudan He, Weifeng Shi
Summary: This study used a proteomic technique to investigate the changes in the protein and phosphoprotein profiles of human rhabdomyosarcoma cells during EV-A71 infection. The study identified significant alterations in host proteins and phosphosites upon viral infection, affecting pathways related to protein translation, cell cycle control, and cell survival. Additionally, the study discovered conserved phosphorylation sites in viral polyproteins that were previously unreported.
JOURNAL OF PROTEOME RESEARCH
(2022)
Article
Biochemical Research Methods
Martin Klammer, Klaus Godl, Andreas Tebbe, Christoph Schaab
BMC BIOINFORMATICS
(2010)
Article
Biochemistry & Molecular Biology
Michael A. D'Elia, Kathryn E. Millar, Amit P. Bhavsar, Ana M. Tomijenovic, Bernd Hutter, Christoph Schaab, Gabriel Moreno-Hagelsieb, Eric D. Brown
CHEMISTRY & BIOLOGY
(2009)
Article
Biochemical Research Methods
Sibylle Guendisch, Kathrin Grundner-Culemann, Claudia Wolff, Christina Schott, Bilge Reischauer, Manuela Machatti, Daniel Groelz, Christoph Schaab, Andreas Tebbe, Karl-Friedrich Becker
JOURNAL OF PROTEOME RESEARCH
(2013)
Article
Biochemical Research Methods
Felix S. Oppermann, Martin Klammer, Caroline Bobe, Juergen Cox, Christoph Schaab, Andreas Tebbe, Henrik Daub
JOURNAL OF PROTEOME RESEARCH
(2013)
Article
Biochemical Research Methods
Martin Klammer, Marc Kaminski, Alexandra Zedler, Felix Oppermann, Stephanie Blencke, Sandra Marx, Stefan Mueller, Andreas Tebbe, Klaus Godl, Christoph Schaab
MOLECULAR & CELLULAR PROTEOMICS
(2012)
Article
Biochemical Research Methods
Tamar Geiger, Anja Wehner, Christoph Schaab, Juergen Cox, Matthias Mann
MOLECULAR & CELLULAR PROTEOMICS
(2012)
Article
Biochemical Research Methods
Christoph Schaab, Tamar Geiger, Gabriele Stoehr, Juergen Cox, Matthias Mann
MOLECULAR & CELLULAR PROTEOMICS
(2012)
Article
Biochemical Research Methods
Gabriele Stoehr, Christoph Schaab, Johannes Graumann, Matthias Mann
MOLECULAR & CELLULAR PROTEOMICS
(2013)
Article
Biochemical Research Methods
Nina Maeusbacher, Thiemo B. Schreiber, Manuela Machatti, Christoph Schaab, Henrik Daub
Article
Oncology
Laura Conradt, Klaus Godl, Christoph Schaab, Andreas Tebbe, Stefan Eser, Sandra Diersch, Christoph W. Michalski, Joerg Kleeff, Angelika Schnieke, Roland M. Schmid, Dieter Saur, Guenter Schneider
Article
Microbiology
B Hutter, C Schaab, S Albrecht, M Borgmann, NA Brunner, C Freiberg, K Ziegelbauer, CO Rock, I Ivanov, H Loferer
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2004)
Article
Microbiology
B Hutter, C Fischer, A Jacobi, C Schaab, H Loferer
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2004)
Article
Biochemistry & Molecular Biology
AW Machl, C Schaab, I Ivanov
NUCLEIC ACIDS RESEARCH
(2002)