Article
Medicine, Research & Experimental
Dong Zhou, Yanping He, Hengping Li, Weidong Huang
Summary: The study investigated the impact of KLK6 on the proliferation, migration, and invasion of gastric cancer cells, finding that KLK6 inhibition could suppress cell proliferation, migration, and invasion by inhibiting EMT.
EXPERIMENTAL AND THERAPEUTIC MEDICINE
(2021)
Article
Biochemical Research Methods
Yingying Ma, Songbiao Zhu, Meiqi Yi, Wenhao Zhang, Yuanyuan Xue, Xiaohui Liu, Haiteng Deng
Summary: Protein S-glutathionylation is an important post-translational modification that regulates cellular processes. This study investigated the changes in glutathionylome during epithelial-mesenchymal transition (EMT) and found that the glutathionylation level of 15-hydroxyprostaglandin dehydrogenase (15-PGDH) increased, inhibiting its enzymatic activity and promoting EMT.
JOURNAL OF PROTEOME RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Woodys Lenga Ma Bonda, Marion Lavergne, Virginie Vasseur, Lucie Brisson, Sebastien Roger, Antoine Legras, Antoine Guillon, Serge Guyetant, Pieter S. Hiemstra, Mustapha Si-Tahar, Sophie Iochmann, Pascale Reverdiau
Summary: KLK5 has been identified as a crucial protease in extracellular matrix remodeling and bronchial epithelial repair after injury. It promotes epithelial-to-mesenchymal transition (EMT) and cell migration by activating the mitogen-activated protein kinase signaling pathway, while also delaying wound healing repair.
Review
Biochemistry & Molecular Biology
Ming-Yu Chou, Muh-Hwa Yang
Summary: The interplay between EMT and immune metabolic changes in cancer cells during tumor metastasis has gained interest among researchers. Understanding how they influence each other may provide novel explanations for immune surveillance during metastasis. Studying immunometabolism in the regulatory loop between tumor-infiltrating immune cells and EMT is crucial for cancer treatment.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Immunology
Tiantian Wu, Caijin Tang, Renchuan Tao, Xiangzhi Yong, Qiaozhi Jiang, Cong Feng
Summary: The research found that PD-L1 is highly expressed in oral squamous cell carcinoma (OSCC), correlated with worse survival, and related to macrophage infiltration and EMT process. Patients with high PD-L1 expression may benefit from ICI treatment and show higher sensitivity to certain chemical drugs.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Oncology
Valerio Ciccone, Arianna Filippelli, Chiara Bacchella, Enrico Monzani, Lucia Morbidelli
Summary: This study investigates the antitumor effects of a novel nitric oxide (NO) donor, [Zn(PipNONO)Cl], on cancer cells and endothelial cells. The results demonstrate the potential therapeutic role of this NO donor in controlling tumor growth and metastasis by affecting epithelial-mesenchymal transition (EMT) and endothelial-mesenchymal transition (EndMT) processes.
Review
Oncology
Barbora Kvokackova, Jan Remsik, Mohit Kumar Jolly, Karel Soucek
Summary: Epithelial-mesenchymal transition (EMT) and mesenchymal-epithelial transition (MET) play critical roles in the development of TNBC, shaping aggressive and heterogeneous biological features, but may also contribute to the progression of metastatic disease.
Article
Medicine, Research & Experimental
Yan Sun, Lang Xiong, Xiaoran Wang, Liping Wang, Baoxin Chen, Jingqi Huang, Mi Huang, Jieping Chen, Jing Wu, Shan Huang, Yizhi Liu
Summary: This study demonstrated that TGF-beta 2 induces autophagy flux during EMT progression of LECs, with autophagy activation enhancing fibrogenic responses and cell migration, while autophagy inhibition alleviates EMT markers and cell migration. Additionally, modulation of Smad signaling by autophagy was observed, and ATG7-silenced LECs showed antifibrosis effect induced by TGF-beta 2. Overall, intervention/inhibition of autophagy could attenuate TGF-beta 2-induced EMT in LECs, providing insights for preventing and treating fibrotic cataracts and other fibrotic diseases.
Article
Pharmacology & Pharmacy
Jingnan Zhang, Ze Zhang, Zhenlin Huang, Manlin Li, Fan Yang, Zeqi Wu, Qian Guo, Xiyu Mei, Bin Lu, Changhong Wang, Zhengtao Wang, Lili Ji
Summary: This study reported that a natural compound isotoosendanin (ITSN) reduced TNBC metastasis by inhibiting TGF-P-induced EMT and the formation of invadopodia. ITSN can directly interact with TGF-P receptor type-1 (TGFPR1) and abrogated the kinase activity of TGFPR1, thereby blocking the TGF-P-initiated downstream signaling pathway. Moreover, ITSN also improved the inhibitory efficacy of programmed cell death 1 ligand 1 (PD-L1) antibody for TNBC in vivo via inhibiting the TGF-P-mediated EMT in the tumor microenvironment.
ACTA PHARMACEUTICA SINICA B
(2023)
Review
Biochemistry & Molecular Biology
Joanna Kozak, Alicja Forma, Marcin Czeczelewski, Pawel Kozyra, Elzbieta Sitarz, Elzbieta Radzikowska-Buchner, Monika Sitarz, Jacek Baj
Summary: Epithelial-mesenchymal transition (EMT) is a key process in the development of gastric cancer, affecting patient outcomes. There is an urgent need for research on pharmacological agents targeting EMT to prevent progression and potential metastasis of gastric cancer.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Chien-Hung Lin, Chuan Wan, Wen-Sheng Liu, Hsin-Hui Wang
Summary: Exposure to PM2.5 can induce EMT characteristics in HK-2 cells, with the IL-6/STAT3 pathway playing a key role in mediating early renal tubule cell injury.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Toxicology
Mahsa Ghorbaninejad, Anna Meyfour, Samaneh Maleknia, Shabnam Shahrokh, Meghdad Abdollahpour-Alitappeh, Hamid Asadzadeh-Aghdaei
Summary: This study revealed that increased SHH signaling activity in patients with inflammatory bowel disease enhanced inflammatory responses and affected epithelial cell characteristics. Inhibiting SHH signaling may have therapeutic benefits in treating inflammatory conditions in the intestines.
TOXICOLOGY IN VITRO
(2022)
Article
Biochemistry & Molecular Biology
Zirong Deng, Yun Xu, Yuchen Cai, Weiling Lin, Libei Zhang, Aoqing Jiang, Yuhang Zhou, Rui Zhao, Heyan Zhao, Zhaoguo Liu, Tingdong Yan
Summary: This study found that RRP15 expression was associated with overall survival and disease-free survival in colon cancer patients. In vitro experiments showed that knockdown of RRP15 inhibited the growth, colony-forming ability, and invasive ability of colon cancer cells, while overexpression of RRP15 enhanced these oncogenic functions. In vivo experiments in nude mice further confirmed that RRP15 knockdown inhibited tumor growth. Additionally, RRP15 knockdown inhibited the epithelial-mesenchymal transition (EMT) in colon cancer cells. It suggests that RRP15 inhibition may be a promising therapeutic target for colon cancer.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Longxiyu Meng, Jie Gao, Wenjing Mo, Baojun Wang, Hongwei Shen, Wenmin Cao, Meng Ding, Wenli Diao, Wei Chen, Qing Zhang, Jiaxin Shu, Huiqi Dai, Hongqian Guo
Summary: This study reveals that MIOX is a crucial regulatory molecule in ccRCC, which impacts the growth, metastasis, and prognosis of the disease.
Article
Environmental Sciences
Yan Wang, Siling Li, Jing Zhao, Qiuyue Li, Chunjie Xu, Huihui Wu, Zhonghui Zhu, Lin Tian
Summary: The study revealed that partial EMT may be involved in the development of pulmonary fibrosis diseases, with Snail playing a critical role in the process. The process of EMT in epithelial cells promoted the differentiation of lung myofibroblasts, and this epithelial-mesenchymal crosstalk was mainly controlled by Snail.
Article
Chemistry, Multidisciplinary
Georgios Angelis, Golfo G. Kordopati, Eleni Zingkou, Anastasia Karioti, Georgia Sotiropoulou, Georgios Pampalakis
Summary: Research suggests that Enceladus, one of Saturn's satellites, may have a soda-type subsurface ocean with conditions potentially able to support life, as well as an iron ore-based core. The ocean chemistry related to Enceladus could support the development of Fe-based hydrothermal vents, which are thought to be potential cradles of life. Chemical structures formed in this environment can catalyze the transformation of simple organic prebiotic molecules into complex polymers, indicating that basic biochemical precursors may have emerged in Enceladus.
CHEMISTRY-A EUROPEAN JOURNAL
(2021)
Review
Biochemistry & Molecular Biology
Georgios Pampalakis, Eleni Zingkou, Christos Panagiotidis, Georgia Sotiropoulou
Summary: Kallikrein-related peptidases (KLKs) have been found to be important regulators of viral infections, facilitating the entry of viruses like influenza, coronaviruses, and papillomavirus into host cells through proteolysis. Apart from directly activating viruses, KLK5 and 12 can also promote viral entry indirectly through proteolytic cascade events. Their potential as host targets for developing new antivirals has been highlighted in recent studies.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2021)
Article
Developmental Biology
Eleni Zingkou, Georgios Pampalakis, Georgia Sotiropoulou
Summary: This study highlights the role of abnormal epidermal differentiation and proteolysis in skin diseases such as Netherton syndrome, seborrheic dermatitis, and psoriasis. The expression of specific proteins and enzymes associated with differentiation and proteolysis is altered in these diseases, providing insights into their pathology and potential treatment options.
INTERNATIONAL JOURNAL OF DEVELOPMENTAL BIOLOGY
(2022)
Article
Immunology
Eleni Zingkou, Georgios Pampalakis, Georgia Sotiropoulou
Summary: This study found that a combination therapy of anti-TNF alpha and anti-KLK5 can effectively treat Netherton syndrome, providing evidence for this potential treatment.
JOURNAL OF CLINICAL IMMUNOLOGY
(2022)
Review
Oncology
Georgios Pampalakis, Georgios Angelis, Eleni Zingkou, Kostas Vekrellis, Georgia Sotiropoulou
Summary: ALS is a fatal disease involving degeneration of motor neurons. Multiple causative genes have been identified, indicating diverse pathways leading to ALS. Chemical entities are a promising choice to delay ALS progression, but have shown no efficacy in clinical trials. Exploiting the chemical entity-genotype correlation could guide patient stratification for treatment.
CLINICAL AND TRANSLATIONAL MEDICINE
(2022)
Letter
Oncology
Georgia Sotiropoulou, Eleni Zingkou, Georgios Pampalakis
CANCER BIOLOGY & THERAPY
(2022)
Article
Biochemistry & Molecular Biology
Stavroula Kostoudi, Georgios Pampalakis
Summary: Compounds with phosphorus-carbon (P-C) bonds have significant pharmacological, biochemical, and toxicological properties. The Michaelis-Arbuzov reaction, a notable method for forming P-C bonds, has undergone modifications and expansions in its application, resulting in a broader range of reactants and products.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Oncology
Georgia Sotiropoulou, Eleni Zingkou, Georgios Pampalakis
Summary: This article discusses the regulatory mechanisms of epidermal proteolysis and its importance in skin physiology and disease states. Recent studies have revealed the complexity of epidermal proteolytic and signaling pathways, as well as their association with lipid metabolism.
JOURNAL OF PATHOLOGY
(2022)
Review
Pharmacology & Pharmacy
Georgia Sotiropoulou, Eleni Zingkou, Evangelos Bisyris, Georgios Pampalakis
Summary: Proteases are regulated enzymes that can be detected and quantified using activity-based probes (ABPs), which have potential applications in molecular imaging, diagnosis, and therapy.
Article
Chemistry, Multidisciplinary
Georgios Angelis, Georgios Sant, Ioannis S. Vizirianakis, Georgios Pampalakis
Summary: Chemobrionic architectures are generated by slowly injecting aqueous silicate solution into gaseous TiCl4. These structures were characterized using XRD, SEM, and wet chemistry control experiments, and their formation mechanism was discovered. They serve as laboratory models for studying the formation of calthemites or soda straws.
CHEMICAL COMMUNICATIONS
(2023)
Article
Medicine, Research & Experimental
Anna Woestemeier, Pasquale Scognamiglio, Yu Zhao, Jonas Wagner, Franziska Muscate, Christian Casar, Francesco Siracusa, Filippo Cortesi, Theodora Agalioti, Simone Mueller, Adrian Sagebiel, Leonie Konczalla, Ramez Wahib, Karl-Frederick Karstens, Anastasios D. Giannou, Anna Dupree, Stefan Wolter, Milagros N. Wong, Anne K. Muhlig, Agata A. Bielecka, Vikas Bansal, Tianran Zhang, Oliver Mann, Victor G. Puelles, Tobias B. Huber, Ansgar W. Lohse, Jakob R. Izbicki, Noah W. Palm, Stefan Bonn, Samuel Huber, Nicola Gagliani
Summary: This study reveals the role of CD4(+) T cells with a Th17 polarization state in the progression of nonalcoholic fatty liver disease (NAFLD) to nonalcoholic steatohepatitis (NASH) and the development of fibrosis. The study also suggests a potential contribution of the gut-liver axis in NASH, with Bacteroides enrichment in the intestine correlating with the frequency of multicytokine-producing CD4(+) T cells.
Review
Biochemistry & Molecular Biology
Georgios Pampalakis, Stavroula Kostoudi
Summary: The study provides a comprehensive overview of V-agents, categorizing them based on their structures to facilitate further research. It also describes the production, physical properties, toxicity, and storage stability of V-agents. The high toxicity and stability of V-agents make them a serious percutaneous hazard, with the potential to contaminate exposed areas for weeks.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Multidisciplinary
Georgios Angelis, Maria-Eleni Katsanou, Alexandros Giannopoulos-Dimitriou, Ioannis S. Vizirianakis, Georgios Pampalakis
Summary: CaCl2 addition to a highly alkaline phosphate buffer generates submerged transparent chemobrionic bubbles mimicking jellyfish that are stable and malleable. Regular vertical chemical gardens can grow from the bubble through a gas micro-rocket propelled mechanism triggered by a compartmentalized O-2-generating reaction. The bubbles can mechanically separate to yield two daughter bubbles in a process resembling cytokinesis or natural jellyfish regeneration, and then re-grow through new injection of CaCl2. Finally, E. coli bacteria genetically engineered to produce green fluorescence were loaded inside the bubbles to create a biomimetic symbiotic analogue.
Article
Chemistry, Organic
Evangelos Bisyris, Eleni Zingkou, Golfo G. Kordopati, Minos Matsoukas, Plato A. Magriotis, Georgios Pampalakis, Georgia Sotiropoulou
Summary: Kallikrein 7 (KLK7) is a chymotrypsin-like serine protease with established roles in skin diseases, and a new mixed alkyl aryl phosphonate quenched activity-based probe has been developed to potentially monitor its activity.
ORGANIC & BIOMOLECULAR CHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Evangelos Bisyris, Eleni Zingkou, Golfo G. Kordopati, Minos Matsoukas, Plato A. Magriotis, Georgios Pampalakis, Georgia Sotiropoulou
Summary: A new in silico approach was used to design a kallikrein 7 (KLK7)-specific phosphonate activity-based probe (ABP) for quantifying active KLK7. The epidermal application of the ABP-inhibitor on Netherton syndrome model mice reversed disease hallmarks, showcasing the potential of using ABPs as theranostics.
CHEMICAL COMMUNICATIONS
(2021)