期刊
CANCER LETTERS
卷 352, 期 2, 页码 220-227出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.canlet.2014.06.018
关键词
Adipose tissue-derived stem cells; MCF-7; IFN-beta; High density culture; Growth suppression
类别
资金
- Basic Science Research Program through the National Research Foundation of Korea (NRF) - Ministry of Education [NRF-2012R1A1A2004981]
Although it has been reported that mesenchymal stem cells (MSCs) suppress tumor growth in vitro and in vivo, little is known about the underlying molecular mechanisms. We found that type I interferon is expressed in adipose tissue-derived stem cells (ASCs) cultured at high density, and ASCs and their conditioned medium (ASC-CM) suppress the growth of MCF-7 cells in vitro. Growth inhibition was amplified by glucose deprivation that resulted from high density culture of ASCs after 3 days. The cytotoxic effect of the ASC-CM obtained from high density culture of ASCs was neutralized by anti-IFN-beta antibody. STAT1 was phosphorylated in MCF-7 cells treated with ASC-CM, and JAK1/JAK2 inhibitor treatment decreased STAT1 phosphorylation. The cytotoxic effect of ASC-CM was reduced especially by JAK1 inhibitors in MCF-7 cells. Our findings suggest that ASCs cultured at high density express type I interferons, which suppresses tumor growth via STAT1 activation resulting from IFN-beta secretion in MCF-7 breast cancer cells. (C) 2014 Elsevier Ireland Ltd. All rights reserved.
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