期刊
CANCER LETTERS
卷 318, 期 2, 页码 173-179出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.canlet.2011.12.017
关键词
Leukemia; Leukemic stem cell; NOD/SCID mice; Natural killer cell; NKG2D ligand
类别
资金
- Guangdong Provincial Natural Science Fund [7001196]
- Guangdong Provincial Science and Technology Program [2009B030801245]
- Guangdong Provincial Traditional Chinese Medicine Administration [2007180]
Leukemic stem cells (LSCs) play the central role in the relapse and refractory of acute myeloid leukemia (AML) and highlight the critical need for the new therapeutic strategies to directly target the LSC population. However, relatively little is known about the unique molecular mechanisms of drug and natural killer cells (NK)-killing resistance of LSCs because of very small number of LSCs in bone marrow. In this study, we investigated whether established leukemia cell line contains LSCs. We showed that KG1a leukemia cell line contained leukemic stem-like cells, which have been phenotypically restricted within the CD34(+)CD38(-) fractions. CD34(+)CD38(-) cells could generate CD34(+)CD38(+) cells in culture medium and had renewal function. Moreover. CD34(+)CD38(-) cells had self-renewal potential. We found that leukemic stem-like cells from KG1a cells were resistant to chemotherapy and NK-mediated cytotoxicity. NKG2D ligands involve in protecting LSCs from NK-mediated attack. Taken together, our studies provide a novel cell model for leukemic stem cells research. Our data also shed light on mechanism of double resistant to chemotherapy and NK cell immunotherapy, which was helpful for developing novel effective strategies for LSCs. (C) 2011 Elsevier Ireland Ltd. All rights reserved.
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