Article
Pharmacology & Pharmacy
Vera Vysochinskaya, Sergey Shishlyannikov, Yana Zabrodskaya, Elena Shmendel, Sergey Klotchenko, Olga Dobrovolskaya, Nina Gavrilova, Darya Makarova, Marina Plotnikova, Ekaterina Elpaeva, Andrey Gorshkov, Dmitry Moshkoff, Mikhail Maslov, Andrey Vasin
Summary: The design of cationic liposomes composed of 2X3 and DOPE can greatly enhance mRNA delivery for mRNA-based therapies. The study investigated the effect of 2X3-DOPE composition and lipid-to-mRNA ratio (N/P) on mRNA transfection. It was found that lipoplex-mediated transfection efficiency relies on both liposome composition and N/P ratio, with liposomes 2X3-DOPE (1:3) showing superior delivery compared to Lipofectamine MessengerMax.
Article
Pharmacology & Pharmacy
Jose Antonio Lebron, Pilar Lopez-Cornejo, Elena Garcia-Dionisio, Pablo Huertas, Margarita Garcia-Calderon, Maria Luisa Moya, Francisco Jose Ostos, Manuel Lopez-Lopez
Summary: The tendency of surfactants to self-aggregate plays a key role in determining their efficiency for compacting nucleic acids, with 12NBr showing the most effectiveness as a DNA compacting agent. Lipoplexes of DOPE/12NBr/pEGFP-C1 were prepared and characterized, with investigations into their cytotoxicity and transfection efficiency.
Article
Nanoscience & Nanotechnology
Liefeng Hu, Shuting Li, Yonggang Lv
Summary: In this study, a pH-sensitive linear poly(ortho ester) polycation was successfully prepared and used for gene transfection in neuroma cells. The polymer showed good biocompatibility and highly specified transfection efficiency on human neuroblastoma cells, with serum tolerance and deeper transfection depth. Compared with the control group, this polymer demonstrated great potential in neuroma therapy.
ACS APPLIED NANO MATERIALS
(2023)
Article
Pharmacology & Pharmacy
Sohail Akhter, Mathieu Berchel, Paul-Alain Jaffres, Patrick Midoux, Chantal Pichon
Summary: This study investigated the characteristics of cationic liposomes as carriers for mRNA delivery. The results showed that specific co-lipids could enhance the transfection efficiency of mRNA, and the acid-mediated membrane fusion activity of liposomes was not strongly correlated with the transfection level. Additionally, the study found a negative correlation between mRNA density and translation efficiency after release. The development of methods to maintain mRNA translation activity is crucial.
Article
Peripheral Vascular Disease
Juan Pablo Robles, Magdalena Zamora, Lourdes Siqueiros-Marquez, Elva Adan-Castro, Gabriela Ramirez-Hernandez, Francisco Freinet Nunez, Fernando Lopez-Casillas, Robert P. Millar, Thomas Bertsch, Gonzalo Martinez de la Escalera, Jakob Triebel, Carmen Clapp
Summary: The study revealed that the hormone prolactin generates vasoinhibin with therapeutic potential against diseases, and identified the short linear motif HGR as the functional determinant which can effectively inhibit angiogenesis and vasopermeability.
Article
Biochemistry & Molecular Biology
Yu-Rong Zhan, Ping Chen, Xi He, Meng-Wei Hei, Ji Zhang, Xiao-Qi Yu
Summary: In this study, a dual gene delivery system based on sodium alginate-doping cationic nanoparticles was developed for gene therapy. The results showed that the nanoparticles exhibited good biocompatibility and high gene transfection efficiency. The codelivery of p53 and KillerRed reporter genes using the nanoparticles resulted in a synergistic effect, showing potential for photodynamic therapy.
Article
Chemistry, Multidisciplinary
Marie Halaskova, Filip Kostelansky, Jiri Demuth, Ingrid Hlbocanova, Miroslav Miletin, Petr Zimcik, Miloslav Machacek, Veronika Novakova
Summary: Effective interaction with biomembranes is crucial for the activity of photosensitizers. In this study, amphiphilic cationic phthalocyanines with different bulkiness of substitution on the lipophilic part (-H, -SMe, -StBu) were prepared and their interaction with biomembranes was investigated. The results showed that even the bulky -StBu derivatives could enter biomembranes and exhibited good photosensitizing activity. Moreover, these derivatives demonstrated excellent anti-tumor activity in various cell lines.
Review
Biotechnology & Applied Microbiology
Kai Ma, Chun-Liu Mi, Xiang-Xiang Cao, Tian-Yun Wang
Summary: Gene delivery systems are crucial for gene therapy and recombinant protein production, with non-viral vector gene delivery reagents offering advantages such as large packaging load capacity and low immunogenicity. Liposomes and non-liposome cationic polymers are commonly used as gene delivery reagents, with different head structures including quaternary ammonium salt, amine, amino acid, polypeptide, guanidine salt, and a heterocyclic ring.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Pharmacology & Pharmacy
Yu-Jen Lu, Hao-Lung Hsu, Yu-Hsiang Lan, Jyh-Ping Chen
Summary: Thermosensitive cationic magnetic liposomes (TCMLs) were prepared for controlled drug/gene release in cancer treatment. The liposomes were made from DPPC, cholesterol, PEGylated lipid, and DDAB, and co-entrapped MNPs and CPT-11. The drug release was triggered by temperature increase or magneto-heating, and the liposomes showed enhanced cytotoxicity and gene silencing effect in glioblastoma cells.
Article
Pharmacology & Pharmacy
Daniil V. Gladkikh, Aleksandra V. Sen'kova, Ivan V. Chernikov, Tatyana O. Kabilova, Nelly A. Popova, Valery P. Nikolin, Elena V. Shmendel, Mikhail A. Maslov, Valentin V. Vlassov, Marina A. Zenkova, Elena L. Chernolovskaya
Summary: The study shows that liposome F is not toxic in healthy mice and can accumulate rapidly in tumors with high folate receptor levels. Combined therapy including anti-MDR1 siRNA/F complexes and polychemotherapy can significantly inhibit the growth of multidrug-resistant tumors without inducing liver toxicity.
Review
Pharmacology & Pharmacy
Jeroen Heuts, Wim Jiskoot, Ferry Ossendorp, Koen van der Maaden
Summary: Cationic nanoparticles have shown to be effective in cancer immunotherapy by delivering tumor-associated antigens to dendritic cells and inducing strong antigen-specific cellular immune responses. Lipid- and polymer-based cationic nanoparticles are utilized for different types of cancer vaccines, and their potential in T-cell induction is discussed for therapeutic cancer vaccination.
Article
Pharmacology & Pharmacy
Amanda C. Carita, Jacqueline Resende de Azevedo, Yves Chevalier, Delphine Arquier, Marcus Buri, Karin A. Riske, Gislaine Ricci Leonardi, Marie-Alexandrine Bolzinger
Summary: The study investigates the influence of hydrophilic surfactants on the membrane elasticity of liposomes and their effect on the skin absorption of vitamin C. Encapsulation of vitamin C inside cationic liposomes is aimed at improving its delivery to the skin. Elastic liposomes (ELs) formed by the addition of Polysorbate 80 to conventional liposomes (CLs) were compared. The presence of a positive charge in the liposomal membrane significantly increases the encapsulation efficacy of both CLs and ELs, indicating a potential mechanism involving interactions between cationic lipids and vitamin C during skin diffusion.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2023)
Article
Biochemistry & Molecular Biology
Rejhana Kolasinac, Dirk Bier, Laura Schmitt, Andriy Yabluchanskiy, Bernd Neumaier, Rudolf Merkel, Agnes Csiszar
Summary: The study focused on the development of cationic fusogenic liposomes with intercalated radioactive [I-131]I- for potential therapeutic applications. Experiment results showed that these liposomes, with high membrane fusion ability, had a special delivery pathway and exhibited excellent delivery efficiency in in vitro experiments, suitable for invasive cancer cell models.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Materials Science, Multidisciplinary
Hui Liu, Xiaoyun Zhao, Yu Bai, Huichao Xie, Zhen Yang, Yichen Wang, Yongfeng Chen, Yucen Luo, Mengrui Ma, Wenwen Lu, Jizhuang Ma, Tianzhi Yang, Bo Jin, Pingtian Ding
Summary: In this study, a class of amphiphilic polymer vectors with different hydrophobicities and biodegradability were synthesized to improve gene delivery efficiency and reduce cytotoxicity through hydrophobic modification. These vectors could form flexible and uniform nanoparticles, enhance endocytosis efficiency, and demonstrate higher transfection efficiency. Moreover, the hydrophobic structural domain of the vectors improved serum stability and exhibited the ability to inhibit tumor growth in breast cancer models.
MATERIALS & DESIGN
(2022)
Article
Chemistry, Physical
Dipanjan Mukherjee, Md Nur Hasan, Ria Ghosh, Gourab Ghosh, Arpan Bera, Sujanthi Easwara Prasad, Ankita Hiwale, Praveen K. Vemula, Ranjan Das, Samir Kumar Pal
Summary: Complexes of cationic liposomes with DNA have shown promise as nonviral vectors for gene therapy. This study examines the kinetics of liposome/DNA complex formation and reveals two different kinetic processes using novel cationic lipids. The observed difference in kinetics is likely due to variations in the lipid/DNA complex structures.
JOURNAL OF PHYSICAL CHEMISTRY B
(2022)
Article
Respiratory System
Makon-Sebastien Njock, Julien Guiot, Monique A. Henket, Olivier Nivelles, Marc Thiry, Franck Dequiedt, Jean-Louis Corhay, Renaud E. Louis, Ingrid Struman
Article
Oncology
Pierre Foidart, Cassandre Yip, Jean Radermacher, Silvia Blacher, Mehdi Lienard, Laetitia Montero-Ruiz, Erik Maquoi, Elodie Montaudon, Sophie Chateau-Joubert, Joelle Collignon, Michel Coibion, Veronique Jossa, Elisabetta Marangoni, Agnes Noel, Nor Eddine Sounni, Guy Jerusalem
CLINICAL CANCER RESEARCH
(2019)
Article
Oncology
Charline Dubois, Natacha Rocks, Silvia Blacher, Irina Primac, Anne Gallez, Melissa Garcia-Caballero, Celine Gerard, Laurent Brouchet, Agnes Noel, Francoise Lenfant, Didier Cataldo, Christel Pequeux
ENDOCRINE-RELATED CANCER
(2019)
Article
Medicine, Research & Experimental
Irina Primac, Erik Maquoi, Silvia Blacher, Ritva Heljasvaara, Jan Van Deun, Hilde Y. H. Smeland, Annalisa Canale, Thomas Louis, Linda Stuhr, Nor Eddine Sounni, Didier Cataldo, Taina Pihlajaniemi, Christel Pequeux, Olivier De Wever, Donald Gullberg, Agnes Noel
JOURNAL OF CLINICAL INVESTIGATION
(2019)
Review
Medicine, General & Internal
Julien Guiot, Ingrid Struman, Edouard Louis, Renaud Louis, Michel Malaise, Makon-Sebastien Njock
JOURNAL OF CLINICAL MEDICINE
(2019)
Article
Biochemistry & Molecular Biology
Jennifer Perez-Boza, Amandine Boeckx, Michele Lion, Franck Dequiedt, Ingrid Struman
CELLULAR AND MOLECULAR LIFE SCIENCES
(2020)
Article
Biochemistry & Molecular Biology
Marie-Emilie Francart, Aline M. Vanwynsberghe, Justine Lambert, Morgane Bourcy, Anthony Genna, Julien Ancel, Jennifer Perez-Boza, Agnes Noel, Philippe Birembaut, Ingrid Struman, Myriam Polette, Christine Gilles
Article
Respiratory System
Julien Guiot, Maureen Cambier, Amandine Boeckx, Monique Henket, Olivier Nivelles, Fanny Gester, Edouard Louis, Michel Malaise, Franck Dequiedt, Renaud Louis, Ingrid Struman, Makon-Sebastien Njock
Article
Oncology
Maureen Van de Velde, Marie Ebroin, Tania Durre, Marc Joiret, Lionel Gillot, Silvia Blacher, Liesbet Geris, Frederic Kridelka, Agnes Noel
Summary: This study revealed a new mechanism by which tumor exposed-LEC promote tumor cell proliferation, showcasing their role in enhancing cancer cell invasion and proliferation in vivo. The analysis of teLEC secretome identified IL6 as a key molecule modulated in teLEC, with neutralization of IL6 abolishing the mitogenic effects mediated by teLEC in vivo. The findings suggest a novel function for teLEC beyond their traditional role in lymphatic vessel formation, emphasizing their potential as key stromal determinants in future studies.
Review
Biochemistry & Molecular Biology
Lionel Gillot, Louis Baudin, Loic Rouaud, Frederic Kridelka, Agnes Noel
Summary: Lymph node metastasis is a significant prognostic factor in various cancers, serving as a gateway for further dissemination. Prior to metastasis, the primary tumor reshapes the draining lymph node by secreting soluble factors or releasing extracellular vesicles, creating a pre-metastatic niche for the survival and growth of metastatic cells. This review highlights the structure of lymph nodes, matrix composition, cellular heterogeneity, and major processes associated with nodal pre-metastatic niche formation, offering insights into potential research directions and clinical implications.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Gilles Parzibut, Monique Henket, Catherine Moermans, Ingrid Struman, Edouard Louis, Michel Malaise, Renaud Louis, Benoit Misset, Makon-Sebastien Njock, Julien Guiot
Summary: This study analyzed miRNA expression in plasma-derived exosomes of ARDS patients through small RNA sequencing, identifying differentially expressed miRNAs and validating their potential as diagnostic biomarkers in discriminating ARDS patients from healthy subjects.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Marie Fontaine, Stephanie Herkenne, Ek Olivier, Alicia Paquot, Amandine Boeckx, Cecile Paques, Olivier Nivelles, Marc Thiry, Ingrid Struman
Summary: This study identifies the significance of two microRNAs in the communication between endothelial cells and vascular smooth muscle cells through extracellular vesicles, and reveals their role in regulating cell phenotypes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Lionel Gillot, Alizee Lebeau, Louis Baudin, Charles Pottier, Thomas Louis, Tania Durre, Remi Longuespee, Gabriel Mazzucchelli, Christophe Nizet, Silvia Blacher, Frederic Kridelka, Agnes Noel
Summary: This study revealed an association between POSTN deposition and lymphangiogenesis in lymph nodes of cervical cancer patients, highlighting the key role of POSTN in promoting lymphatic vessel formation and the seeding of metastatic cells.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Fabrice Gucciardo, Sebastien Pirson, Louis Baudin, Alizee Lebeau, Agnes Noel
Summary: The urokinase plasminogen activator receptor-associated protein (uPARAP/Endo180) is a key protein involved in collagen internalization and degradation, playing a role in pathologies like fibrosis and cancer. Recent research has also shown its importance in vascular biology and immune system regulation.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Article
Cell Biology
Quentin Roblain, Thomas Louis, Cassandre Yip, Louis Baudin, Ingrid Struman, Vincenza Caolo, Vincent Lambert, Julie Lecomte, Agnes Noel, Stephane Heymans
Summary: Age-related macular degeneration (AMD) is a leading cause of blindness in individuals over 50 years old worldwide, with wet AMD being the most aggressive form characterized by specific features and pathological processes. MiR-142-3p plays a key role in CNV formation, highlighting its importance in the progression of the disease.
Review
Oncology
Xinru Zhou, Yin Jia, Chuanbin Mao, Shanrong Liu
Summary: Small extracellular vesicles (sEVs), such as exosomes, have emerged as crucial targets for liquid biopsy and promising drug delivery vehicles in tumor progression. They can serve as biomarkers for tumor diagnosis and as drug carriers for cancer treatment.
Article
Oncology
Ruochan Chen, Ju Zhu, Xiao Zhong, Jie Li, Rui Kang, Daolin Tang
Summary: The interplay between autophagy and apoptosis plays a crucial role in tumorigenesis and cancer therapy, with HMGB1 serving as a key regulator in these processes.
Article
Oncology
Zongfu Pan, Xixuan Lu, Tong Xu, Jinming Chen, Lisha Bao, Ying Li, Yingying Gong, Yulu Che, Xiaozhou Zou, Zhuo Tan, Ping Huang, Minghua Ge
Summary: This study uncovered the emerging role of HN1 in promoting dedifferentiation of anaplastic thyroid cancer (ATC) cells. HN1 negatively regulated the thyroid differentiation markers and had an inhibitory effect on the transcriptional activation of CTCF, thereby influencing the chromatin accessibility of thyroid differentiation genes.
Article
Oncology
Yi Qin, Shengjun Xiong, Jun Ren, Gautam Sethi
Summary: Autophagy plays an important regulatory role in glioblastoma, and its dysregulation can lead to drug resistance and radioresistance. It also affects stem cell characteristics, overall growth, and metastasis. Therefore, autophagy is a promising target for glioblastoma therapy.
Article
Oncology
Katsuya Nagaoka, Xuewei Bai, Dan Liu, Kevin Cao, Joud Mulla, Chengcheng Ji, Hongze Chen, Muhammad Azhar Nisar, Amalia Bay, William Mueller, Grace Hildebrand, Jin-Song Gao, Shaolei Lu, Hiroko Setoyama, Yasuhito Tanaka, Jack R. Wands, Chiung-Kuei Huang
Summary: This study found that serum 2-OG levels in cholangiocarcinoma patients are associated with the effectiveness of chemotherapy. Patients with progressive disease showed significantly higher levels of serum 2-OG compared to stable disease and partial response patients. The study also revealed that overexpression of ASPH mimics the effects of 2-OG, and knockdown of ASPH improves chemotherapy. Targeting ASPH enhances the effects of chemotherapy by modulating ATM and ATR, two key regulators of DDRs.