期刊
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
卷 740, 期 -, 页码 8-13出版社
ELSEVIER SCIENCE SA
DOI: 10.1016/j.jelechem.2014.12.037
关键词
Acetylcholinesterase; Covalent immobilization; Graphene nanoribbon; Pesticide; Biosensor
资金
- National Natural Science Foundation of China [21175061, 21375050]
- Key Laboratory of Modern Agriculture Equipment and Technology [NZ201109]
- Qing Lan and the Priority Academic Program Development of Jiangsu Higher Education Institutions [PAPD-2014-37]
This work described a sensitive electrochemical biosensor for the detection of carbaryl pesticide based on covalent immobilization of acetylcholinesterase (AChE) on the multiwall carbon nanotubes/graphene oxide nanoribbons (MWCNTs/GONRs) nanostructure. The catalytic activity of AChE immobilized on the MWCNTs/GONRs film was superior to that of MWCNTs due to the covalent binding technique, and the biosensor showed high affinity to acetylthiocholine with a Michaelis-Menten constant value of 0.25 mM. Based on the inhibition of carbaryl on the enzymatic activity of the immobilized AChE, the resulting biosensor exhibited superior performance for carbaryl detection including good reproducibility, acceptable stability, and wide linear range from 5 nM up to 5000 nM with a low detection limit of 1.7 nM. The biosensor was successfully challenged with real sample demonstrating to be a useful analytical tool for insecticide detection. (C) 2014 Elsevier B.V. All rights reserved.
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