Article
Chemistry, Analytical
Ya Wang, Tai Ye, Min Yuan, Hui Cao, Jingsong Yu, Fengqin Yin, Xiuxiu Wu, Liling Hao, Fei Xu
Summary: A fluorescent aptasensor was developed for the rapid detection of Pb2+ using a G-quadruplex structure and fluorescence quenching technology. The method showed a good linear relationship and low detection limit under optimal conditions, and demonstrated satisfactory recovery rates in spiked lettuce samples. This target-induced PET platform has potential for further expansion to analyze other heavy metals.
Article
Nanoscience & Nanotechnology
Qian Wang, Huanshun Yin, Jia Ding, Yunlei Zhou, Shiyun Ai
Summary: The study developed a photoelectrochemical biosensor based on WS2/Bi/BiOBr nanocomposites, black TiO2 (b-TiO2), and hemin/G-quadruplex for the highly sensitive and selective detection of 5-formyluracil-2'-deoxyuridine-5'-triphosphate (5fdUTP). The biosensor showed a linear range of 0.005-500 nM and a detection limit of 1.53 pM, with enhanced PEC response by the hemin/G-quadruplex structure. The method exhibited optimistic selectivity through precision and accuracy evaluation with standard addition recovery experiments.
ACS APPLIED NANO MATERIALS
(2021)
Article
Chemistry, Multidisciplinary
Anthony R. Monte Carlo, Jinglin Fu
Summary: Reliable catalysis is crucial for the synthesis of chemicals, molecular sensing, and biomedicine. The G-quadruplex/Hemin (GQH) complex, a peroxidase-mimicking DNAzyme, has been widely used, but its kinetics is unstable. This study investigates the factors causing the inactivation of GQH and signal degradation during long reaction periods, proposing suggestions for optimizing GQH-catalyzed reactions and improving catalytic reliability. The results have implications for GQH-related applications.
Article
Chemistry, Analytical
Hanxiao Wang, Mengyan Wang, Jinzhi Liu, Gaiping Li, Baoxian Ye, Lina Zou
Summary: An ultrasensitive photoelectrochemical (PEC) analysis method based on terminal deoxynucleotidyl transferase (TdT) amplification effect and G-quadruplex/hemin (G4/hemin) catalytic reaction was developed for the determination of ampicillin. The method showed a wide detection range and low limit of detection, making it a promising tool for the analysis of ampicillin.
ANALYTICA CHIMICA ACTA
(2022)
Article
Chemistry, Analytical
Jingyi Dong, Xia Qiu, Mimi Huang, Xiaohu Chen, Yongxin Li
Summary: A new sensing platform based on G-quadruplex-hemin DNAzyme (GQH) functionalized gold-coated nanopipettes was established. GQH was immobilized on the gold-coated nanopipette and acted as a catalyst for the reaction of H2O2 with ABTS to promote the conversion of ABTS to ABTS+ ions inside the nanopipette, allowing real-time monitoring of transmembrane ion current changes. The correlation between ion current and H2O2 concentration in a certain range enables the use of this immobilized nanopipette for hydrogen peroxide sensing.
Article
Chemistry, Analytical
Hao Wang, Cheng Yang, Haoran Tang, Yongxin Li
Summary: This study developed an electrochemical amplification strategy to monitor the electrocatalytic behavior of single G-quadruplex/hemin, and designed a hybridization chain reaction strategy for miRNA-15 detection. These provide a meaningful approach for nucleic acid sensing at the single molecule/particle level with good selectivity and sensitivity.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Juan Li, Meilisha Xu, Xiangyi Huang, Jicun Ren
Summary: This paper systematically investigates the effects of reactants and catalysts on the efficiency of CRET using CL imaging. The results show that the concentration of the catalyst hemin/G-quadruplex has a significant impact on the CRET efficiency, while the concentration of the CL reactants has no significant effect. Additionally, controlling the distance between the donor and acceptor using a DNA double-stranded structure reveals a decrease in CRET efficiency with increasing distance.
Article
Chemistry, Analytical
Xiaobo Zhang, Fengya Wang, Hui Zhi, Peng Wan, Liang Feng
Summary: This study reports a label-free colorimetric sensor that integrates a 3D paper-based analytical device and a smartphone for rapid and sensitive detection of ochratoxin A (OTA) residues in food samples. The paper-based analytical device enables specific recognition of OTA and self-assembly of G-quadruplex (G4)/hemin DNAzyme, and converts the OTA binding event signal into a colorimetric signal. The device exhibited a detection range of 0.1-500 ng/mL and a detection limit of 41.9 pg/mL for OTA, with good results obtained in spiked real samples.
Article
Chemistry, Multidisciplinary
Jiayu Zhang, Xiaofang Song, Miaoren Xia, Yanan Xue, Mengxue Zhou, Lifo Ruan, Huiru Lu, Jun Chen, Dongqi Wang, Zhifang Chai, Yi Hu
Summary: The study found an unexpected difference in the DNAzyme activity between in vitro assays and in mitochondria, with molecular dynamic simulations illustrating how the interaction of G-quadruplex with hemin could modulate the DNAzyme activity. These results may aid in better understanding the catalytic mechanism of DNAzymes and in designing stable and active DNAzymes for intracellular catalysis.
CHEMICAL COMMUNICATIONS
(2021)
Article
Chemistry, Physical
Petr Stadlbauer, Barira Islam, Michal Otyepka, Jielin Chen, David Monchaud, Jun Zhou, Jean-Louis Mergny, Jiri Sponer
Summary: Guanine quadruplex nucleic acids (G4s) play important roles in biological processes and have biotechnological applications. Molecular dynamics simulations reveal complex interaction mechanisms between hemin and G4, suggesting unique biocatalytic properties.
JOURNAL OF CHEMICAL THEORY AND COMPUTATION
(2021)
Article
Chemistry, Physical
Wenqin Zhou, Rui Lai, Yu Cheng, Yu Bao, Wenhui Miao, Xupeng Cao, Guoqing Jia, Guohui Li, Can Li
Summary: In this study, the effect of NH4+ ions on the catalytic behavior of the dimeric G4/Hemin DNAzyme was investigated. It was found that NH4+ ions increased the accumulation of compound I (Cpd I) in the dimeric DNAzyme process. Molecular dynamics simulations showed that NH4+ ions could stabilize the dimeric G4 and interact with the substrate hydrogen peroxide through hydrogen bonds. The interaction between NH4+ ions and H2O2 contributed to the stability of intermediate and transition-state species involved in the catalytic formation of Cpd I.
Article
Polymer Science
Cheng Deng, Hai Yang, Simin Liu, Zhiyong Zhao
Summary: In this study, two types of DNA-dendrimer amphiphiles were successfully constructed, including the G-quadruplex-dendrimer hybrid (G20-Den) and single-stranded DNA-dendrimer hybrid (D20-Den). Both of them self-assembled into nanofibers with a length of several micrometers in MES buffer, and the diameter of G20-Den nanofibers was smaller than that of D20-Den nanofibers due to the compact stacking of G-quadruplex formed in G20-Den hybrids. The catalytic properties of G20-Den nanofiber/hemin, as well as the single-stranded G20/hemin system, were investigated, and they exhibited higher catalytic peroxidation activity than D20-Den nanofiber/hemin. Furthermore, the recycle of these nanofibers can be simply achieved through simple ultrafiltration.
Article
Biochemistry & Molecular Biology
Yun Zhang, Xinao Ma, Jingtian Zhang, Feixian Luo, Wenshu Wang, Xiaojie Cui
Summary: This study introduced a dimeric G-quadruplex structure into DNAzyme for the development of a high-sensitivity biosensor targeting norovirus nucleic acids. The catalytic activity was enhanced through the synergistic effect of hemin, providing a cost-effective and simple-operating colorimetric biosensing method for the detection of target nucleic acids.
Article
Chemistry, Analytical
Zongbing Li, Huanwen Xu, Shiqiang Li, Shujie Wu, Xiangmin Miao
Summary: A multi-signal synergistically amplified electrochemical sensing method for HIV DNA was proposed, utilizing a 2D DNA-Au nanowire structure, hemin/G-quadruplex, and PCR. By combining various signal amplification processes, ultra-sensitive detection of HIV DNA was achieved, demonstrating potential for clinical diagnosis.
ANALYTICA CHIMICA ACTA
(2021)
Article
Nanoscience & Nanotechnology
Xuanxiang Mao, Dehui Qiu, Shijiong Wei, Xiaobo Zhang, Jianping Lei, Jean-Louis Mergny, Huangxian Ju, Jun Zhou
Summary: This study reports a new dual-Hemin-G-quadruplex DNAzyme and its integration with glucose oxidase (GOx) within a hydrophilic metal-organic framework MAF-7 for a biocatalytic cascade system. The system shows high catalytic activity, good protective performance, and excellent recyclability, making it suitable for biosensing and industrial processes.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Optics
Lijun Xu, Xianyue Liu, Peng Zhang, Xiaohua Wang
Summary: This paper investigates the simulation and experiment of non-line-of-sight underwater wireless optical communication (NLOS UWOC), which utilizes scattering of small particles for communication in turbid seawater. The experimental results match the simulation results, verifying the correctness of the model.
JOURNAL OF OPTICS-INDIA
(2023)
Article
Biochemistry & Molecular Biology
Ying Liu, Wanqiu Liu, Siqi Fan, Yuanyuan Yu, Zhaoxia Shi, Wenyuan Liu, Zongyao Wu, Yuzhi Jiao, Huiling Tang, Jie Zhang, Lijun Xu, Feng Feng, Jian Xu
Summary: In this study, UPLC/MS and GNPS methods were used to predict chemical compositions of fermented medicine. The dynamic changes of strains, chemical compositions and anti-inflammatory activity during fermentation process were investigated, and the interactions between strains, chemical compositions and efficacy were revealed. The potential strains related to the production of anti-inflammatory ingredients were identified.
CHEMISTRY & BIODIVERSITY
(2023)
Review
Biochemistry & Molecular Biology
Mengru Li, Lijun Xu, Rui Zhang, Chunming Dong
Summary: ASXL2, a transcription regulator, is a hot topic in tumor detection. Aberrant expression of ASXL2 protein is mainly associated with malignant hematological and heart diseases. To explore the predictive value of ASXL2 in diseases, we reviewed its structure, function, post-translational modification mechanism, and expression in different disease pathogenesis, providing a theoretical basis and support for future treatments.
CURRENT PROTEIN & PEPTIDE SCIENCE
(2023)
Article
Energy & Fuels
Fangyan Li, Lijun Xu, Lijun Yang, Zhang Cao
Summary: This paper proposes a method for describing the thermo-acoustic coupling process in a Helmholtz pulse combustor based on three thermo-acoustic-related parameters. The results indicate that these parameters interact and couple, influencing the self-excited pulsation process.
Article
Engineering, Electrical & Electronic
Xiaokai Zhang, Jiangtao Sun, Jinhai Hu, Peng Suo, Xingbin Liu, Ronghua Xie, Bing Cai, Liang Sun, Tao Wang, Chenwei Wang, Lijun Xu
Summary: Due to long-term exploitation of oil wells, high water-cut oil-water two-phase flow has become common in offshore oil fields. This study analyzes and compares two cross correlation flowmeters, the transverse excitation mode flowmeter and the axial excitation mode flowmeter, for this type of flow. The resolutions and sensitivities of the sensors are compared theoretically, numerically, and experimentally to demonstrate their ability to detect oil bubbles in water. The results show that the transverse excitation mode flowmeter has higher resolution and sensitivity than the axial excitation mode flowmeter.
IEEE SENSORS JOURNAL
(2023)
Article
Engineering, Electrical & Electronic
Zining Wang, Xiaolu Li, Tengfei Bi, Duan Li, Lijun Xu
Summary: A compact lab-built LiDAR is designed and constructed, featuring a programmable-scanning-pattern image-space scanner and a Gauss-Newton (GN) iteration-based in situ ranging system. The angular resolution of the lab-built LiDAR is evaluated using a trapezoid-fitting method, which shows satisfactory performance when compared with a commercial LiDAR. Experimental results demonstrate that the lab-built LiDAR achieves high ranging accuracy, small angular error, and fine angular resolution. The proposed LiDAR and evaluation method have potential applications in hazard detection and avoidance in aerospace.
IEEE TRANSACTIONS ON INSTRUMENTATION AND MEASUREMENT
(2023)
Article
Engineering, Chemical
Zaixing Liu, Zhiyang Ma, Qiuya Tu, Shijie Sun, Lijun Xu, Ruihuan Ge, Wuqiang Yang, Haigang Wang
Summary: In this study, a non-intrusive measurement approach combining electrical capacitance tomography (ECT), pressure measurements, and high-speed CCD imaging technology was proposed to investigate the spouted flow characteristics in a rectangular spout-fluid bed with bafflers. The results showed that the flow regime in the jet region transited from slugging to spouting during the fountain formation process, and the flow regime in the same region changed to slugging bed when the fluidization velocity in one side increased. The spouting pressure drop was affected by the fluidization condition in the side region, and the high characteristic frequency of the pressure signal decreased when the side region condition changed from blockage to fluidization. The fountain height increased linearly with the superficial gas velocity and tended to be offset by the influence of the lateral airflow caused by the imbalance of the air pressure on two side regions.
CHEMICAL ENGINEERING SCIENCE
(2023)
Article
Chemistry, Multidisciplinary
Lijun Xu, Ruslan A. Rymzhanov, Pengfei Zhai, Shengxia Zhang, Peipei Hu, Xuan Meng, Jian Zeng, Youmei Sun, Jie Liu
Summary: In this study, a new method to directly fabricate size- and density-controllable sub-10 nm nanopores in WO3 nanosheets using single swift heavy ions (SHIs) without any chemical etching process was demonstrated. Nanopores with sizes from 1.8 to 7.4 nm can be created in WO3 nanosheets by selecting ions of different electronic energy losses (Se). The viscosity and surface tension of the transient molten phase caused by SHIs were proposed as the key factors for the formation of nanopores. This method provides a way to fabricate solid-state nanopores in materials with a low viscosity and surface tension.
Review
Endocrinology & Metabolism
Lingjun Rong, Lixia Zhang, Zaigang Yang, Lijun Xu
Summary: Bone-related diseases are a major health burden, and stem cell therapy has emerged as an effective strategy for tissue repair. However, the identity and definition of skeletal stem cells remain controversial. Recent progress in purifying skeletal stem cells and understanding their role in bone development and repair, as well as the impact of aging, has been made through advances in technology.
OSTEOPOROSIS INTERNATIONAL
(2023)
Article
Materials Science, Multidisciplinary
Pan Zhang, Lijun Xu, Pengzhao Shi, Fan Yang, Minglin Wang
Summary: A cellular automaton-finite element model and secondary dendrite arm spacing (SDAS) model were established to investigate the solidification structure and secondary dendrites of 2311 die steel slab. The experimental results of nail shooting and acid etching were used to validate the temperature field and solidification structure. The mathematical model and simulation results were used to obtain the SDAS model, which was consistent with the metallographic microscope observation. The effects of slab thickness on equiaxed crystal ratio (ECR) and SDAS were studied under the same water flow and superheat, and their impact on macrosegregation was analyzed.
Article
Engineering, Electrical & Electronic
Peng Suo, Jiangtao Sun, Xiaokai Zhang, Xiaolin Li, Shijie Sun, Lijun Xu
Summary: This article presents an adaptive group-based sparse representation scheme for image reconstruction in electrical capacitance tomography. The proposed method accurately recovers the shape and location of abnormities by exploiting local self-similarity and the sparsity of the image in the form of a group. It achieves more sparsity and less computation by using sparse coefficients and representation matrices obtained via adaptive self-learning. The method shows potential in fulfilling high requirements for temporal and spatial resolutions in dynamic process imaging.
IEEE TRANSACTIONS ON INSTRUMENTATION AND MEASUREMENT
(2023)
Article
Engineering, Electrical & Electronic
Yudong Guo, Lijun Xu, Hongyao Li, Hongyu Zhang, Zhang Cao
Summary: This study introduces a planar laser-induced fluorescence (PLIF) technique that can be used to image the temperature and concentration distribution inside flames. The proposed method utilizes laser absorption spectroscopy (LAS) tomography to provide initial images of gas temperature, which are then used to update the fluorescence image iteratively. The results of numerical simulations and experiments demonstrate the effectiveness of this method, with relative errors of temperature and concentration within acceptable ranges.
IEEE TRANSACTIONS ON INSTRUMENTATION AND MEASUREMENT
(2023)
Article
Engineering, Electrical & Electronic
Fanghao Lu, Lijun Xu, Yigong Wu, Zhang Cao
Summary: A novel downsampling scheme was proposed to directly extract a precise and bias-immune absorption spectrum for dynamic gas temperatures. Multiple direct absorption profiles were generated by downsampling the absorbed wavelength-modulated laser intensity at equal phases in each modulation cycle. These profiles were employed to remove intensity biases from thermal radiations and obtain an entire absorption spectrum. Experimental results showed that the proposed method achieved higher precision and noise reduction compared to direct absorption spectroscopy and wavelength modulation spectroscopy methods. It also successfully evaluated the temporal variations of an acoustically excited flame.
IEEE TRANSACTIONS ON INSTRUMENTATION AND MEASUREMENT
(2023)
Article
Engineering, Electrical & Electronic
Shuang Qiu, Zhang Cao, Jinting Wen, Xiaoqian Zhang, Lijun Xu
Summary: A discrete tomographic imaging method is proposed for reconstructing temperature and gas concentration distributions using tunable diode laser absorption spectroscopy. The method discretizes the continuously distributed temperature and gas concentration into finite sets and approximates the distributions using value discretization. A linear matrix equation is used to solve for temperature and concentration values, and projections along laser paths are determined from measured absorption spectra. Numerical simulations and experiments demonstrate the effectiveness of the proposed method in obtaining accurate images with improved noise immunity.
IEEE TRANSACTIONS ON INSTRUMENTATION AND MEASUREMENT
(2023)
Article
Engineering, Electrical & Electronic
Jiangtao Sun, Xiaolin Li, Xu Bai, Shijie Sun, Lijun Xu
Summary: This article presents the measurement of transient flash evaporation flow in liquefied gas propulsion (LGP) using a dual-plane electrical capacitance tomography (ECT) sensor. The study introduces a method for more accurate liquid holdup measurement, an adaptive cross correlation algorithm for stable velocity measurement, and compensation methods to account for temperature and pressure changes. Simulations and experiments validated the effectiveness of the proposed methods in deriving the mass flow rate of the two-phase flow. The research has important implications for online control of LGP and estimating the remaining life of small spacecrafts.
IEEE TRANSACTIONS ON INSTRUMENTATION AND MEASUREMENT
(2023)
Article
Chemistry, Analytical
Gang Qu, Yuxin Zhao, Qiaoli Zhang, Jina Wu, Xiaosen Li, Yang Yang, Shilei Liu
Summary: In this study, magnetic mesoporous materials combined with real-time in situ mass spectrometry were used for the high-throughput detection of hydrolyzed products of organophosphorus nerve agents. The method showed good linearity, low limits of detection and quantification, and high extraction recoveries. The magnetic preparation method used was quick, cost-effective, rugged, and safe. The results demonstrated the potential of this method for rapid and efficient determination of the target analytes in environmental samples.
ANALYTICAL METHODS
(2024)
Article
Chemistry, Analytical
Anna Hildebrand, Mariam Merchant, Danny O'Hare
Summary: Substandard and falsified artemisinin derivatives in antimalarials have caused significant deaths and economic losses. This study evaluates the feasibility of voltammetric methods for identifying and quantifying artemether. The findings suggest that electrochemical analysis shows promise as a method for artemether identification and quantification.
ANALYTICAL METHODS
(2024)
Article
Chemistry, Analytical
Marx Osorio Araujo Pereira, Alvaro Ferreira Junior, Edson Silvio Batista Rodrigues, Helena Mulser, Giovanna Nascimento de Mello e Silva, Wallans Torres Pio dos Santos, Eric de Souza Gil
Summary: Brazilian spotted fever (BSF) is a serious and rapidly evolving disease. A new impedimetric immunosensor was developed for rapid diagnosis by measuring specific antibodies in plasma. The sensor demonstrated selectivity and accuracy, and has potential for important applications in diagnostic testing.
ANALYTICAL METHODS
(2024)
Article
Chemistry, Analytical
Kathrin Schilling, Ronald A. Glabonjat, Olgica Balac, Marta Galvez-Fernandez, Arce Domingo-Relloso, Vesna Slavkovich, Jeff Goldsmith, Miranda R. Jones, Tiffany R. Sanchez, Ana Navas-Acien
Summary: Analysis of trace elements in urine is an important tool for assessing exposures, diagnosing nutritional status, and guiding public health and healthcare intervention. This study provides a sensitive method for analyzing 18 elements in urine samples, using only 100 μL. The results show good accuracy and sensitivity of the method.
ANALYTICAL METHODS
(2024)
Article
Chemistry, Analytical
Mengya Li, Shijie Liu, Shiliang Guo, Dong Liang, Miaoyun Li, Yaodi Zhu, Lijun Zhao, Jong-Hoon Lee, Gaiming Zhao, Yangyang Ma, Yanxia Liu
Summary: In this study, a magnetic flow device was developed to purify spores in a culture medium system. The device used magnetic nanoparticles to absorb vegetative cells, separating them from the spores. The achieved purity of the collected spores was over 95%. The study also demonstrated a rapid quantitative detection method using Raman spectroscopy.
ANALYTICAL METHODS
(2024)
Article
Chemistry, Analytical
Wanqiong Liu, Zixuan Wu, Jianwei Peng, Zebin Xu, Yong Liang
Summary: Metal-organic frameworks (MOFs) are effective carriers for molecular imprinting, but their poor dispersibility in aqueous solution is a significant drawback. In this study, we have applied amphiphilic block copolymers and molecularly imprinted technology on MOFs to improve the hydrophilicity of molecularly imprinted fluorescent materials.
ANALYTICAL METHODS
(2024)
