Article
Biochemistry & Molecular Biology
Jinguo Zhang, Wencai Guan, Xiaolin Xu, Fanchen Wang, Xin Li, Guoxiong Xu
Summary: The study reveals that Sorcin plays a key role in chemoresistance of ovarian cancer, potentially driving PTX resistance through miR-142-5p and ZEB1. Targeting the SRI/Smad4/ZEB1/miR-142-5p loop may reverse chemoresistance.
Article
Biochemistry & Molecular Biology
Mai Koizumi Ichikawa, Kaori Endo, Yuka Itoh, Asami Hotta Osada, Yujiro Kimura, Koichiro Ueki, Kunio Yoshizawa, Keiji Miyazawa, Masao Saitoh
Summary: The epithelial-mesenchymal transition (EMT) plays a crucial role in epithelial tumor progression. EMT transcription factors Snail and ZEB1/2 are key regulators of this transition. TGF-beta and active Ras signals induce the expression of Snail, while Ets1 and Ets2 contribute to the upregulation of both Snail and ZEB1/2. Ets1 is a crucial molecule for regulating Snail and ZEB1/2, and cancer progression is mediated through post-translational modification of the exon VII domain.
Article
Biochemistry & Molecular Biology
Christopher S. Pulford, Chandana K. Uppalapati, McKale R. Montgomery, Richard L. Averitte, Elizabeth E. Hull, Kathryn J. Leyva
Summary: Studying patient-derived cSCC and adjacent normal tissues revealed a hybrid EMT process within individual cSCC tumors, where cells at the tumor edges displayed a more mesenchymal phenotype, while cells in the center maintained higher expression of epithelial markers. This dynamic may be regulated by miRNA and associated transcription factors, impacting therapeutic strategies for more invasive disease.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Medicine, Research & Experimental
Ning Zhao, Minwei He, Wei Chen, Peng Jin, Lulu Cao, Jinhai Deng, Xu Cheng, Lu Wang
Summary: Our study identified FAM96A as a crucial regulator of tumor metastasis and epithelial-mesenchymal transition (EMT) by inhibiting the TGF-beta signaling pathway.
Review
Biochemistry & Molecular Biology
Naoyuki Taniguchi, Yuki Ohkawa, Kento Maeda, Yoichiro Harada, Masamichi Nagae, Yasuhiko Kizuka, Hideyuki Ihara, Yoshitaka Ikeda
Summary: Cancer-related changes in glycans on cell surface proteins are regulated by glycosyltransferases and related genes, which play key roles in cancer metastasis and invasion. The significance of EMT and N-glycan branching glycosyltransferases needs to be reconsidered for potential therapeutic strategies. Specific glycosyltransferases involved in EMT or MET pathways may be difficult to define due to their roles in intermediate states.
MOLECULAR ASPECTS OF MEDICINE
(2021)
Article
Multidisciplinary Sciences
Katsuhiko Warita, Takuro Ishikawa, Akihiro Sugiura, Jiro Tashiro, Hiroaki Shimakura, Yoshinao Z. Hosaka, Ken-ichi Ohta, Tomoko Warita, Zoltan N. Oltvai
Summary: Statins, such as atorvastatin, can delay metastasis formation in vivo and attenuate the growth and proliferation of tumor cells, especially in those with a mesenchymal-like phenotype. In epithelial cancer cells treated with TGF-beta, atorvastatin-induced growth inhibition is stronger, and pre-treatment with atorvastatin can enhance this effect further by reducing HMGCR expression. Targeting HMGCR pharmacologically can strongly inhibit the growth of epithelial cancer cells treated with TGF-beta and potentially improve statin therapy-mediated attenuation of metastasis formation in vivo.
SCIENTIFIC REPORTS
(2021)
Article
Multidisciplinary Sciences
Benjamin Nordick, Mary Chae-Yeon Park, Vito Quaranta, Tian Hong
Summary: Cooperative RNA degradation and microRNAs play an important role in generating multiple intermediate EMT states, bridging the gap between mechanistic models and single-cell experiments.
Article
Cell Biology
Yan Guo, Xiaoqin Lu, Yao Chen, Geoff Clark, John Trent, Miriam Cuatrecasas, Douglas Emery, Zhao-Hui Song, Julia Chariker, Eric Rouchka, Antonio Postigo, Yongqing Liu, Douglas C. Dean
Summary: This study shows a cytoplasmic-to-nuclear transport gradient of the EMT transcription factor Zeb1 in cancer cells, which is regulated by the EMT inducer Tgfb. The transport of Zeb1 promotes the assembly of the actin cytoskeleton and cell migration. Inhibition of Zeb1 nuclear transport can suppress the malignant transition of cancer cells.
Article
Biochemistry & Molecular Biology
Long Xu, Jian Wang, Buhan Liu, Jiaying Fu, Yuanxin Zhao, Sihang Yu, Luyan Shen, Xiaoyu Yan, Jing Su
Summary: Epithelial ovarian cancer has the highest mortality rate among gynecological malignant tumors, largely due to metastasis. This study found that HDAC9 plays different roles in different histological subtypes of ovarian cancer and may be a potential target for personalized treatment.
Article
Medicine, Research & Experimental
Haoqi Zhao, Lan Wang, Shufang Wang, Xihua Chen, Min Liang, Xin Zhang, Jiedong Wang, Xiangbo Xu
Summary: The study revealed that the overexpression of CHN1 is associated with lymph node metastasis and low survival rates in cervical carcinoma patients. CHN1 overexpression promoted cell proliferation, migration, and invasion by inducing EMT-related transcription factors. Furthermore, in vitro experiments showed that CHN1 overexpression activated the Akt/GSK-3 beta/Snail signaling pathway.
JOURNAL OF TRANSLATIONAL MEDICINE
(2021)
Article
Oncology
Xiaowu Liu, Guannan Zhao, Xueyun Huo, Yaohong Wang, Gabor Tigyi, Bing-Mei Zhu, Junming Yue, Wenjing Zhang
Summary: Adipose-derived stem cells (ADSC) play a promoting role in ovarian cancer metastasis by inducing epithelial to mesenchymal transition (EMT) in OC cells, leading to proliferation, survival, migration, and invasion. Experimental evidence shows that ADSC contributes to ovarian tumor growth and metastasis by activating the TGF-beta pathway. Targeting ADSC with TGF-beta inhibitor has therapeutic potential in blocking EMT and OC metastasis.
FRONTIERS IN ONCOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Ali Dayoub, Artem I. Fokin, Maria E. Lomakina, John James, Marina Plays, Tom Jacquin, Nikita M. Novikov, Rostislav S. Vorobyov, Anastasia A. Schegoleva, Karina D. Rysenkova, Julia Gaboriaud, Sergey V. Leonov, Evgeny V. Denisov, Alexis M. Gautreau, Antonina Y. Alexandrova
Summary: Whole exome sequencing of invasive mammary carcinomas identified the association of PTEN and ZFHX3 mutations. In MCF10A cells, PTEN inactivation induced the formation of large colonies, while ZFHX3 inactivation in PTEN KO increased colony numbers and restored their size. PTEN KO enhanced coordinated cell motility and collective migration, while ZFHX3 KO resulted in uncoordinated cell movement and the expression of the mesenchymal marker vimentin. Double KO cells showed decreased coordination in collective migration, high vimentin levels, and restored linear adhesive junctions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Ophthalmology
Yi Chen, Binxin Wu, Jian Feng He, Jingyao Chen, Zi Wei Kang, Dandan Liu, Junjie Luo, Kexin Fang, Xiaoxu Leng, Haibin Tian, Jingying Xu, Caixia Jin, Jieping Zhang, Juan Wang, Jingfa Zhang, Qingjian Ou, Lixia Lu, Furong Gao, Guo-Tong Xu
Summary: This study successfully induced EMT in RPE cells through prolonged low-density culture, and found that the combination of the ROCK inhibitor Y27632 and the TGF-beta receptor inhibitor RepSox effectively suppressed and reversed the EMT process, maintaining the morphology and function of RPE cells and the retina. This combined therapy could be a new strategy for treating proliferative retinal diseases involving EMT of RPE cells.
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2021)
Article
Oncology
Ling Wang, Yali Miao, Jirui Wen, Juan Cheng, Qiao Wen, Zhiwei Zhao, Jiang Wu
Summary: Methyl vanillate inhibits the proliferation and migration of ovarian cancer cells by suppressing the ZEB2/Snail signaling pathway and epithelial-mesenchymal transition (EMT). It may serve as a promising therapeutic drug for ovarian cancer.
TRANSLATIONAL CANCER RESEARCH
(2023)
Review
Medicine, General & Internal
Masao Saitoh
Summary: Epithelial-mesenchymal transition (EMT) plays a crucial role in cancer progression, associated with invasion, metastasis, generation of tumor stem cells, and resistance to therapy. Transforming growth factor (TGF)-beta acts as a key mediator in the EMT process, initiating and maintaining EMT through signaling pathways and transcription factors.
