期刊
CANCER BIOTHERAPY AND RADIOPHARMACEUTICALS
卷 28, 期 2, 页码 115-123出版社
MARY ANN LIEBERT, INC
DOI: 10.1089/cbr.2012.1268
关键词
BIM; Gefitinib resistance; MEK/ERK signaling pathway; PI3K/AKT signaling pathway
类别
资金
- National Natural Science Foundation [30873023, 81071922]
Recent evidence indicates that both the phosphatidylinositol 3-kinase (PI3K)/AKT and the MEK/ERK pathways are strictly regulated by epidermal growth factor receptor in non-small cell lung cancer (NSCLC) that responds to Gefitinib. Gefitinib resistance is partly owing to the activation of two major downstream signaling pathways PI3K/AKT or MEK/ERK. In this study, we found that in Gefitinib-sensitive cell lines, Gefitinib could induce tumor cell apoptosis via upregulation of a proapoptotic protein BIM. Small interfering RNA results showed that silencing of BIM could alleviate apoptosis induced by Gefitinib. We adopted a combination of PI3K inhibitor (LY294002) and MEK inhibitor (U0126) against Gefitinib resistance in cell lines. As expected, the combination substantially induced apoptosis and restored the sensitivity to Gefitinib by increasing the expression of BIM. Our studies provided a theoretical basis for overcoming drug resistance in NSCLC via combination therapy.
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