Article
Chemistry, Analytical
Shengjun Bu, Qianyu Zhou, Liming Liu, Chunyang Zhou, Zhuo Hao, Jiayu Wan, Chunying Pang
Summary: The present study describes a novel technology called TPIA that enables target nucleic acid detection. By using specific design strategy and fluorescence techniques, this system provides a versatile tool with great potential for biomedical research and clinical diagnosis.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Review
Food Science & Technology
Tai-Yong Kim, Xiaoning Zhu, Se-Min Kim, Jeong-A Lim, Min-Cheol Lim, Ke Luo
Summary: Viruses are major pathogens in food poisoning, and the development of foodborne virus detection technologies for the entire food distribution chain is crucial for food safety. Polymerase chain reaction (PCR) is the gold standard method for monitoring food contamination by viruses, but it is time-consuming and vulnerable to inhibitors in food samples. Optical and electrical readout biosensors based on nucleic acid isothermal amplification technology and nanomaterials have emerged as alternatives, offering portability and on-site operation. However, the complexity of food components and lack of stability studies on residual food components limit the practical application of biosensors as a universal detection method.
FOOD RESEARCH INTERNATIONAL
(2023)
Article
Biochemical Research Methods
Noemi Bellassai, Roberta D'Agata, Giuseppe Spoto
Summary: Extracellular miRNAs are potential targets for early diagnosis and prognosis of diseases. This study introduces an innovative strategy for direct detection of miRNAs from biological fluids using hairpin probe-assisted isothermal amplification reaction. The assay was designed and optimized for straightforward analysis of multiple samples using a microfluidic device. By detecting circulating miR-127-5p in synovial fluid, the assay demonstrated its importance in convenient detection of miRNAs in liquid biopsy samples.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Yan Zhao, Xiaomeng Li, Mei-Hao Xiang, Feng Gao, Fengli Qu, Mingfang Li, Limin Lu
Summary: A novel photoelectrochemical biosensor based on an enzyme-free nucleic acid dual-amplification strategy combined with a mimic enzyme has been developed for ultrasensitive detection of miRNA-21. The biosensor achieves outstanding performance with an ultralow detection limit of 33 aM and a wide quantification range from 100 aM to 1 nM. This study opens a new avenue for ultrasensitive detection of miRNAs and has potential applications in clinical and biochemical samples.
Article
Chemistry, Analytical
Qiuyuan Lin, Yuanwei Cao, Guobin Han, Wen Sun, Wenhao Weng, Hui Chen, Haoyi Wang, Jilie Kong
Summary: We report a general and programmable diagnostic platform that combines mesophilic Clostridium perfringens Argonaute (CpAgo) with exponential isothermal amplification (EXPAR) for the rapid and sensitive detection of multiple miRNAs with single-nucleotide discrimination in one pot. The CpAgo-based One-Pot (COP) assay achieved a limit of detection of 1 zMmiRNA within 30 min and showed superior analytical performance in distinguishing colorectal cancer patients from healthy individuals. This programmable, one-pot, multiplex, rapid, and specific strategy holds great promise in scientific research and clinical applications.
ANALYTICAL CHEMISTRY
(2023)
Article
Nanoscience & Nanotechnology
Marta Broto, Michael M. Kaminski, Christopher Adrianus, Nayoung Kim, Robert Greensmith, Schan Dissanayake-Perera, Alexander J. Schubert, Xiao Tan, Hyemin Kim, Anand S. Dighe, James J. Collins, Molly M. Stevens
Summary: This study combines CRISPR-Cas reaction with nanozyme-linked ELISA to achieve quantitative detection of Cas13-mediated RNA detection through catalytic metallic nanoparticles at room temperature (CrisprZyme). The study shows that CrisprZyme is adaptable to lateral flow reading and different Cas enzymes, and can detect various non-coding RNAs. The platform can be used to identify disease patients and monitor cellular differentiation. CrisprZyme can serve as a universally applicable signal catalyst for CRISPR-based diagnostics, expanding the target range of preamplification-free, quantitative detection.
NATURE NANOTECHNOLOGY
(2022)
Article
Chemistry, Analytical
Tong Luo, Jiacheng Li, Yao He, Hui Liu, Zhiwei Deng, Xi Long, Qingqing Wan, Jiacheng Ding, Zan Gong, Yanjing Yang, Shian Zhong
Summary: In this study, we developed a novel CRISPR-based biosensor by combining CRISPR/Cas12a with Au-nanobeacon, which allows for direct and rapid detection of nucleic acids in complex biological fluids with high sensitivity.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Yixia Yang, Jingzhao Yang, Feng Gong, Pengfei Zuo, Zhiyou Tan, Juanjuan Li, Conghua Xie, Xinghu Ji, Wenhua Li, Zhike He
Summary: This study presents a sensing platform based on CRISPR/Cas12a and isothermal exponential amplification reaction (EXPAR) for ultrasensitive analysis of miRNA. The platform provides a wide detection range and low limit of detection, and has the capability to detect other microRNA biomarkers. Additionally, the platform can assess miR-27a levels in breast cancer cell and serum samples, showing promising potential for clinical application in molecular diagnostics.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Review
Pharmacology & Pharmacy
Zahra Hassani Nejad, Fataneh Fatemi, Seyed Ehsan Ranaei Siadat
Summary: Diagnostic testing is crucial in controlling and studying the COVID-19 pandemic. This review discusses various approaches, including nucleic acid and antibody tests, and provides guidelines for more accurate testing.
JOURNAL OF PHARMACEUTICAL ANALYSIS
(2022)
Article
Chemistry, Analytical
Jie Bai, Eryi Shu, Tao Zhang, Hongliang Fan
Summary: MicroRNAs (miRNAs) are small RNA molecules that can be used as diagnostic/prognostic biomarkers and therapeutic targets for diseases. This study proposes an identification-cleavage-amplification (ICA) strategy for highly sensitive and versatile detection of miRNA, successfully applied to miR-155 and miR-21 assays. The strategy combines aligner-target mediated cleavage with strand displacement amplification (ATMC-SDA) for the ICA process.
ANALYTICA CHIMICA ACTA
(2023)
Article
Chemistry, Physical
Yu Wang, Tianhao Yan, Kainan Mei, Depeng Rao, Wenjie Wu, Ye Chen, Yongpei Peng, Jianye Wang, Shangquan Wu, Qingchuan Zhang
Summary: This study developed an ultrasensitive nanomechanical method based on peptide nucleic acid for the detection of SARS-CoV-2 RNA. The method has a low detection limit and can accurately diagnose COVID-19 patients, making it an important tool for early diagnosis and broad detection.
Article
Chemistry, Analytical
Huimin Hao, Ye Li, Bin Yang, Shuyan Lou, Zihua Guo, Weiyi Lu
Summary: In this study, a unique DNA probe with Hill-type cooperativity was developed based on toehold-mediated strand displacement processes, enabling accurate discrimination of single-nucleotide variants. The probe exhibited thermodynamic advantages for specificity, forming mismatch bubbles in the presence of single-nucleotide variants. The probe's feasibility was successfully demonstrated and its design allowed accurate discrimination of single-nucleotide variants, including insertions, mutations, and deletions, with Hill-type cooperativity serving as a novel strategy for DNA probe design.
ANALYTICAL CHEMISTRY
(2023)
Review
Chemistry, Inorganic & Nuclear
Fan Yang, Huiting Lu, Xiangdan Meng, Liping Li, Wenhao Dai, Lingzhi Yang, Ruiping Zhang, Haifeng Dong, Xueji Zhang
Summary: The spatial-temporal and dynamic expression profiles of intracellular microRNA are crucial for understanding cellular biological behaviors and monitoring pathological processes. Nucleic acid probe systems have been developed for various biological tasks in living cells due to their intrinsic biosecurity, biocompatibility, ease of synthesis, and ability for efficient recognition and signal amplification. This review highlights recent achievements in nucleic acid probe systems for intracellular miRNA sensing and discusses approaches for improving detection sensitivity, selectivity, and accuracy.
COORDINATION CHEMISTRY REVIEWS
(2023)
Article
Chemistry, Analytical
Jinjoo Han, Seokhwan Kim, Seokjoon Kim, Eun Sung Lee, Byung Seok Cha, Jung Soo Park, Jiye Shin, Youngjun Jang, Ki Soo Park
Summary: In this study, a strategy using Cas12a protein for active primer production and subsequent isothermal amplification was proposed. The target nucleic acids activated Cas12a to cleave inactive RNA-DNA chimera primers into active ones. Two isothermal amplification methods generated fluorescence and colorimetric signals, allowing effective detection of the Sal-monella enterotoxin gene even in real samples such as drinking water and human serum.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Multidisciplinary Sciences
Helena de Puig, Rose A. Lee, Devora Najjar, Xiao Tan, Luis R. Soekensen, Nicolaas M. Angenent-Mari, Nina M. Donghia, Nicole E. Weckman, Audrey Ory, Carlos F. Ng, Peter Q. Nguyen, Angelo S. Mao, Thomas C. Ferrante, Geoffrey Lansberry, Hani Sallum, James Niemi, James J. Collins
Summary: The miSHERLOCK is a low-cost CRISPR-based POC diagnostic platform that can rapidly detect SARS-CoV-2 and its variants with high sensitivity in just 1 hour. This modular system can be quickly adapted to different viruses and variants, and is complemented by a smartphone application for output interpretation and result reporting.
Article
Chemistry, Analytical
Gang Qu, Yuxin Zhao, Qiaoli Zhang, Jina Wu, Xiaosen Li, Yang Yang, Shilei Liu
Summary: In this study, magnetic mesoporous materials combined with real-time in situ mass spectrometry were used for the high-throughput detection of hydrolyzed products of organophosphorus nerve agents. The method showed good linearity, low limits of detection and quantification, and high extraction recoveries. The magnetic preparation method used was quick, cost-effective, rugged, and safe. The results demonstrated the potential of this method for rapid and efficient determination of the target analytes in environmental samples.
ANALYTICAL METHODS
(2024)
Article
Chemistry, Analytical
Anna Hildebrand, Mariam Merchant, Danny O'Hare
Summary: Substandard and falsified artemisinin derivatives in antimalarials have caused significant deaths and economic losses. This study evaluates the feasibility of voltammetric methods for identifying and quantifying artemether. The findings suggest that electrochemical analysis shows promise as a method for artemether identification and quantification.
ANALYTICAL METHODS
(2024)
Article
Chemistry, Analytical
Marx Osorio Araujo Pereira, Alvaro Ferreira Junior, Edson Silvio Batista Rodrigues, Helena Mulser, Giovanna Nascimento de Mello e Silva, Wallans Torres Pio dos Santos, Eric de Souza Gil
Summary: Brazilian spotted fever (BSF) is a serious and rapidly evolving disease. A new impedimetric immunosensor was developed for rapid diagnosis by measuring specific antibodies in plasma. The sensor demonstrated selectivity and accuracy, and has potential for important applications in diagnostic testing.
ANALYTICAL METHODS
(2024)
Article
Chemistry, Analytical
Kathrin Schilling, Ronald A. Glabonjat, Olgica Balac, Marta Galvez-Fernandez, Arce Domingo-Relloso, Vesna Slavkovich, Jeff Goldsmith, Miranda R. Jones, Tiffany R. Sanchez, Ana Navas-Acien
Summary: Analysis of trace elements in urine is an important tool for assessing exposures, diagnosing nutritional status, and guiding public health and healthcare intervention. This study provides a sensitive method for analyzing 18 elements in urine samples, using only 100 μL. The results show good accuracy and sensitivity of the method.
ANALYTICAL METHODS
(2024)
Article
Chemistry, Analytical
Mengya Li, Shijie Liu, Shiliang Guo, Dong Liang, Miaoyun Li, Yaodi Zhu, Lijun Zhao, Jong-Hoon Lee, Gaiming Zhao, Yangyang Ma, Yanxia Liu
Summary: In this study, a magnetic flow device was developed to purify spores in a culture medium system. The device used magnetic nanoparticles to absorb vegetative cells, separating them from the spores. The achieved purity of the collected spores was over 95%. The study also demonstrated a rapid quantitative detection method using Raman spectroscopy.
ANALYTICAL METHODS
(2024)
Article
Chemistry, Analytical
Wanqiong Liu, Zixuan Wu, Jianwei Peng, Zebin Xu, Yong Liang
Summary: Metal-organic frameworks (MOFs) are effective carriers for molecular imprinting, but their poor dispersibility in aqueous solution is a significant drawback. In this study, we have applied amphiphilic block copolymers and molecularly imprinted technology on MOFs to improve the hydrophilicity of molecularly imprinted fluorescent materials.
ANALYTICAL METHODS
(2024)
