Article
Environmental Sciences
Gerhard P. Aigner, Pamela Nenning, Birgit Fiechtner, Maja Srut, Martina Hoeckner
Summary: This study found that the demethylating agent Aza inhibits gene activity while Cd induces MT2 gene expression, but neither has an effect on global DNA methylation levels. Thus, Cd detoxification based on MT2 induction is not related to changes in DNA methylation.
Article
Agronomy
Shuo Liu, Yu Bao, Hui Deng, Guanqing Liu, Yangshuo Han, Yuechao Wu, Tao Zhang, Chen Chen
Summary: DNA methylation is a crucial epigenetic modification that regulates gene expression and genome stability. The methylation inhibitor, AzaD, can effectively reduce genome-wide hypomethylation and cause a series of epigenetic variations.
Article
Endocrinology & Metabolism
Maria Cristina Carbajo-Garcia, Ana Corachan, Marina Segura-Benitez, Javier Monleon, Julia Escrig, Amparo Faus, Antonio Pellicer, Irene Cervello, Hortensia Ferrero
Summary: The research showed that treatment with 5-aza-CdR can reduce cell proliferation and extracellular matrix formation in uterine leiomyoma cells, as well as inhibit the Wnt/beta-catenin signaling pathway. Therefore, DNA methylation inhibition may be a potential therapeutic target for uterine leiomyoma.
REPRODUCTIVE BIOLOGY AND ENDOCRINOLOGY
(2021)
Article
Oncology
Yang-Jie Zhang, Ran Xie, Jie Jiang, Li Zhai, Cong-Hui Yang, Jing Zhang, Xi Wang, Dong-Xue Chen, Hua-Tao Niu, Long Chen
Summary: The study demonstrates the methylation modification of GAS5 in melanoma and the reversible effect of 5-Aza-dC on this modification. Treatment with 5-Aza-dC inhibits cell proliferation, increases apoptosis, and reduces migration and invasion in melanoma cells. The antitumor effect of 5-Aza-dC in melanoma is related to the methylation modification of GAS5.
Article
Biochemistry & Molecular Biology
Karolina Luczkowska, Piotr Kulig, Klaudia Rusinska, Bartlomiej Baumert, Boguslaw Machalinski
Summary: This study investigates the effects of a methylation inhibitor on the proliferation of multiple myeloma cells and the development of resistance to treatment, finding that alterations in DNA methylation are associated with drug resistance.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Alain Aguirre-Vazquez, Luis A. Salazar-Olivo, Xochitl Flores-Ponce, Ana L. Arriaga-Guerrero, Dariela Garza-Rodriguez, Maria E. Camacho-Moll, Ivan Velasco, Fabiola Castorena-Torres, Nidheesh Dadheech, Mario Bermudez de Leon
Summary: By utilizing epigenetic molecules and small molecules, the expression of pluripotency genes can be induced and methylation in the OCT4 promoter can be reduced in human somatic cells, enhancing reprogramming efficiency. Further studies are needed to elucidate the mechanisms by which these compounds exert their effects on reprogramming.
Review
Genetics & Heredity
Ankita Sharma, Rafeeq Mir, Sanjeev Galande
Summary: This review discusses the role of the Wnt/beta-catenin pathway in cell proliferation, differentiation, and migration, as well as its impact on embryonic development, tissue regeneration, and cancer. It highlights the influence of epigenetic factors on the pathway and the crosstalk with other signaling pathways, particularly the Hippo/YAP pathway. The review also emphasizes recent advances in developing potential therapeutic interventions targeting the epigenetic machinery for treating various cancers and regenerative therapies.
FRONTIERS IN GENETICS
(2021)
Review
Cell Biology
Dipanwita Das, Nandini Karthik, Reshma Taneja
Summary: Inflammation is a crucial immune response against infection and tissue damage, but chronic inflammation is linked to cancer development. Immune cells produce inflammatory mediators to facilitate tumor growth, while aberrant expression of inflammatory factors by tumor cells recruits immune cells for mutual crosstalk, creating a conducive environment for tumor growth.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Pharmacology & Pharmacy
William B. Parker, Jaideep V. Thottassery
Summary: DNMT1 depletion is a rational goal in DNA methylation-dependent disorders, and current agents like decitabine and azacitidine have off-target activities limiting their use. Aza-T-dCyd is a promising candidate with less toxicity and higher therapeutic index for selectively depleting DNMT1. Further research is needed to understand its mechanism of action and potential clinical applications.
JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS
(2021)
Article
Multidisciplinary Sciences
Reem Miari, Naiel Azzam, Rinat Bar-Shalom, Fuad Fares
Summary: 5-Aza-2'-deoxycytidine has shown effectiveness in treating HPV(-) HNSCC by inhibiting proliferation and inducing apoptosis. Future studies are needed to clarify the molecular mechanism of action of 5-AzaD in HPV(-) cancer cells.
Article
Oncology
Ratnakar Singh, Zeeshan Fazal, Emmanuel Bikorimana, Raya Boyd, Cliff Yerby, Megan Tomlin, Hannah Baldwin, Doha Shokry, Andrea K. Corbet, Khadeeja Shahid, Aleyah Hattab, Sarah J. Freemantle, Michael J. Spinella
Summary: TGCTs exhibit reciprocal sensitivity to hypomethylating agents and cisplatin, with resistant cells showing opposite responses. The downregulation of polycomb target gene expression in hypomethylating agent resistant cells contrasts with the upregulation in cisplatin resistant cells, indicating a complex relationship between epigenetic changes and drug sensitivity in TGCTs.
MOLECULAR ONCOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Lu Wang, Zhe Wang, Yuze Zhu, Shutao Tan, Xiaonan Chen, Xianghong Yang
Summary: SOX17 is downregulated in ccRCC, with DNA methylation occurring in the promoter region in a significant portion of ccRCC tissues. Functional experiments demonstrate that SOX17 suppresses proliferation and migration of RCC cells, while negatively regulating the WNT signaling pathway.
ONCOTARGETS AND THERAPY
(2021)
Article
Agriculture, Dairy & Animal Science
Joana Alpoim-Moreira, Anna Szostek-Mioduchowska, Magda Slyszewska, Maria Rosa Rebordao, Dariusz J. Skarzynski, Graca Ferreira-Dias
Summary: Endometrosis is a major cause of infertility in mares, characterized by excessive deposition of collagen and alpha smooth muscle actin in the endometrium. Epigenetic alterations in fibroblast phenotype are linked with the development of endometrial fibrosis. This study investigated the in vitro epigenetic regulation of mare endometrial fibrogenesis through DNMTs transcription and the effect of 5-aza-2'-deoxycytidine on collagen expression in fibroblasts challenged with TGF-beta 1. The findings suggest the potential role of epigenetic mechanisms in equine endometrial fibrogenesis and highlight the therapeutic potential of 5-aza-dC.
Article
Oncology
Jianping Chen, Dandan Wang, Hongqiang Chen, Jin Gu, Xiao Jiang, Fei Han, Jia Cao, Wenbin Liu, Jinyi Liu
Summary: Our study reveals that TMEM196 functions as a novel suppressor of lung cancer metastasis through the Wnt/beta-catenin signaling pathway. TMEM196 mRNA and protein expression levels are significantly decreased in lung cancer tissues and cells. Low expression of TMEM196 is associated with poor prognosis in clinical patients. TMEM196 strongly inhibits tumor metastasis and progression in vitro and in vivo. Mechanistically, TMEM196 inhibits the Wnt signaling pathway and represses beta-catenin promoter transcription. Silencing TMEM196 results in upregulation of beta-catenin and downstream target genes MMP2 and MMP7. Inhibition of beta-catenin expression attenuates the antimetastatic effect of TMEM196 in lung cancer cells.
JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY
(2023)
Article
Cell Biology
Xiaomin Gao, Xiang Shi, Shujian Zhou, Chen Chen, Cai Hu, Qian Xia, Xinlin Li, Wen Gao, Ying Ding, Qisheng Zuo, Yani Zhang, Bichun Li
Summary: Detailed analysis of the regulatory mechanism of spermatogonia stem cell (SSCs) genesis can provide a novel strategy for the application of SSCs in the fields of transgenic animal production and regenerative medicine. This study reveals that Wnt/TCF7L2 signaling pathway promotes SSCs formation by regulating the low DNA methylation of TDRD1 gene.
JOURNAL OF CELLULAR PHYSIOLOGY
(2022)