期刊
CANCER BIOLOGY & THERAPY
卷 9, 期 1, 页码 33-39出版社
LANDES BIOSCIENCE
DOI: 10.4161/cbt.9.1.10279
关键词
marchantin C; glioma; invasion; MAPK; PI3K; STAT3; CAM
类别
资金
- Natural Science Foundation of China [30571696, 30671901, 30628014]
- Ministry of Science and Technology of China [2007AA021000, 2004CB518807]
Cancer cell migration is a leading cause of tumor recurrence and treatment failure. Previously, we reported that marchantin C exhibited promising antitumor activity by inducing microtubule depolymerization and apoptosis. In the present study, we investigated the effect of marchantin C on inhibition of migration in T98G and U87 cells. The scratch-induced migration, Boyden chamber and cell invasion assays were applied to determine that the migrating capacity and invasiveness of these glioma cell lines were inhibited when exposed to marchantin C at a low concentration. There are no obvious signs of apoptosis with this dose. Western blot analyses confirmed that MMP-2, a key role in cancer cell migration, was reduced after incubation with marchantin C in both glioma cell lines. In addition, signaling pathway investigations demonstrated that ERK/MaPK might be involved in MMP-2 downregulation, rather than the AKT/PI3K or JaK/STAT3 pathways. Moreover, marchantin C potently suppressed angiogenesis activity in vivo by CAM assay. This is the first study to demonstrate that marchantin C can inhibit glioma cell migration and invasiveness.
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