Article
Oncology
Toshiyasu Suzuki, Anna Kilbey, Nuria Casa-Rodriguez, Amy Lawlor, Anastasia Georgakopoulou, Hannah Hayman, Kyi Lai Yin Swe, Anna Nordin, Claudio Cantu, Pierre Vantourout, Rachel A. Ridgway, Ryan M. Byrne, Lei Chen, Michael P. Verzi, David M. Gay, Ester Gil Vazquez, Hayley L. Belnoue-Davis, Kathryn Gilroy, Anne Helene Kostner, Christian Kersten, Chanitra Thuwajit, Ditte K. Andersen, Robert Wiesheu, Anett Jandke, Karen Blyth, Antonia K. Roseweir, Simon J. Leedham, Philip D. Dunne, Joanne Edwards, Adrian Hayday, Owen J. Sansom, Seth B. Coffelt
Summary: Colon cancer cells evade immune surveillance by suppressing the expression of y8 T cells and Butyrophilin-like (BTNL) molecules. Reexpression of BTNL enhances the survival and activation of y8 T cells, but it does not affect their cancer-killing ability or recruitment to orthotopic tumors. However, inhibition of 13-catenin signaling through genetic deletion of Bcl9/Bcl9L restores the expression of Hnf4a, Hnf4g, and Btnl genes as well as infiltration of y8 T cells into tumors.
CANCER IMMUNOLOGY RESEARCH
(2023)
Article
Multidisciplinary Sciences
Xin Yu, Wenge Li, Yanjun Feng, Zhijie Gao, Qi Wu, Yue Xia
Summary: Bladder cancer is the second most common urological malignancy and contributes significantly to cancer-related deaths worldwide. The hedgehog signaling pathway plays a role in the onset and spread of various cancers, including bladder cancer. However, there is still limited understanding of the function of HhS in the molecular landscape of bladder cancer.
SCIENTIFIC REPORTS
(2023)
Article
Multidisciplinary Sciences
I-M Launonen, N. Lyytikainen, J. Casado, E. A. Anttila, A. Szabo, U-M Haltia, C. A. Jacobson, J. R. Lin, Z. Maliga, B. E. Howitt, K. C. Strickland, S. Santagata, K. Elias, A. D. D'Andrea, P. A. Konstantinopoulos, P. K. Sorger, A. Farkkila
Summary: In this study, the authors used highly multiplexed imaging to analyze the immune microenvironment of high-grade serous ovarian cancers (HGSOC) and identified phenotypic characteristics and spatial interactions associated with BRCA1/2 gene mutations. These findings have important implications for improving immunotherapeutic strategies and patient stratification.
NATURE COMMUNICATIONS
(2022)
Article
Multidisciplinary Sciences
Pratyusha Bala, Jonathan P. Rennhack, Daulet Aitymbayev, Clare Morris, Sydney M. Moyer, Gina N. Duronio, Paul Doan, Zhixin Li, Xiaoyan Liang, Jason L. Hornick, Matthew B. Yurgelun, William C. Hahn, Nilay S. Sethi
Summary: Cell state plasticity is regulated in adult epithelia to prevent cancer, but its expansion in neoplasia is poorly understood. Mouse models and patient samples revealed that impaired differentiation precedes cancer development, with aberrant transcriptional state marked by Ly6a and reactivation of fetal genes. Genetic inactivation of Sox9 prevents adenoma formation and restores multilineage differentiation. These findings indicate that unabated regenerative activity and developmental reprogramming contribute to the aberrant cell state plasticity preceding cancer development.
Article
Agriculture, Multidisciplinary
Duoduo Zhang, Xingtao Zhou, Linyuan Liu, Mi Guo, Tongwen Huang, Wengan Zhou, Fang Geng, Steve W. Cui, Shaoping Nie
Summary: The study revealed that glucomannan from Aloe vera gel promoted proliferation and differentiation of intestinal stem cells through the Wnt/β-catenin signaling pathway, facilitating epithelial cell regeneration and alleviating colitis in mice.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Health Care Sciences & Services
Aigul R. Rakhmatullina, Rimma N. Mingaleeva, Dina U. Gafurbaeva, Olesya N. Glazunova, Aisylu R. Sagdeeva, Emil R. Bulatov, Albert A. Rizvanov, Regina R. Miftakhova
Summary: This study successfully immortalized adipose-derived MSCs by overexpressing hTERT gene and knocking down TP53, maintaining the differentiation potential and cell morphology stability during long-term culture.
JOURNAL OF PERSONALIZED MEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Mandy Berndt-Paetz, Shanfu Han, Annett Weimann, Annabell Reinhold, Sandra Nuernberger, Jochen Neuhaus
Summary: Three-dimensional tumor models, such as organoids, are valuable tools for bladder cancer research as they accurately mimic the structure and characteristics of solid tumors. These models can be used for functional studies and development of anti-cancer drugs.
Article
Immunology
Mintao Xiao, Xinyi Zhang, Duoli Zhang, Shuai Deng, Anfu Zheng, Fukuan Du, Jing Shen, Lin Yue, Tao Yi, Zhangang Xiao, Yueshui Zhao
Summary: This study reveals the heterogeneity of cancer stem cells (CSCs) in head and neck squamous cell carcinoma (HNSCC) and their underlying mechanisms in the formation of the tumor microenvironment (TME). The functional diversity and signal transduction of CSCs are found to play a crucial role in the progression of HNSCC.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Cell & Tissue Engineering
Wei-Min Chung, Ryan D. Molony, Yi-Fen Lee
Summary: This study revealed that EVs derived from NSCCs contain cargo proteins enriched in proteostasis-related functions, significantly impacting the development of CSCs to be more intrinsically chemoresistant and aggressive. This novel communication mechanism between NSCC-EVs and CSCs may promote disease progression and the acquisition of chemoresistance.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Oncology
Alvin Y. Liu
Summary: The loss of prostate cancer differentiation can lead to an untreatable disease, but the prostate stromal-specific factor PENK has shown potential to induce differentiation and counteract de-differentiation in cancer cells. This suggests that cancer cells may still be responsive to stromal signaling and that differentiation can potentially be induced despite genetic mutations.
Article
Oncology
Osamu Maehara, Goki Suda, Mitsuteru Natsuizaka, Taku Shigesawa, Gouki Kanbe, Megumi Kimura, Masaya Sugiyama, Masashi Mizokami, Masato Nakai, Takuya Sho, Kenichi Morikawa, Koji Ogawa, Shinya Ohashi, Shingo Kagawa, Hideaki Kinugasa, Seiji Naganuma, Naoto Okubo, Shunsuke Ohnishi, Hiroshi Takeda, Naoya Sakamoto
Summary: Fibroblast growth factors (FGFs) and their receptors (FGFRs) play an important role in maintaining cancer stem-like cells in esophageal squamous cell carcinoma (ESCC). This study reveals that FGFR2, particularly the IIIb isoform, is highly expressed in non-CSCs, inducing keratinocyte differentiation and decreasing CSC populations. Analysis using The Cancer Genome Atlas (TCGA) dataset shows a significant correlation between FGFR2 expression and cancer cell differentiation in ESCC clinical samples, suggesting FGFR2-AKT signaling as a potential therapeutic target for ESCC.
CANCER BIOLOGY & THERAPY
(2021)
Article
Oncology
Mustafa Karabicici, Sena Alptekin, Zeynep Firtina Karagonlar, Esra Erdal
Summary: Therapeutic induction of senescence may increase cancer stem cell phenotype, leading to increased tumor aggressiveness and disease recurrence. In liver cancer cells, doxorubicin can induce cellular senescence, increase stem cell gene expression, and cause a shift in stem cell characteristics.
MOLECULAR ONCOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Adriana Buskin, Emma Scott, Ryan Nelson, Luke Gaughan, Craig N. Robson, Rakesh Heer, Anastasia C. Hepburn
Summary: A key challenge in the management of prostate cancer is its heterogeneity, and there is an urgent need for models that can reflect clinical diversity and resistant phenotypes. The three-dimensional organoid model has revolutionized cancer research, providing opportunities for disease modeling, drug screening, and precision medicine. However, there are limitations in emulating the tumor microenvironment and metastasis process, as well as the epigenome profile, which need to be addressed to maximize the translational relevance of organoids.
Article
Chemistry, Multidisciplinary
Jonathan Sai-Hong Chui, Teresa Izuel-Idoype, Alessandra Qualizza, Rita Pires de Almeida, Lindsey Piessens, Bernard K. van der Veer, Gert Vanmarcke, Aneta Malesa, Paraskevi Athanasouli, Ruben Boon, Joris Vriens, Leo van Grunsven, Kian Peng Koh, Catherine M. Verfaillie, Frederic Lluis
Summary: This study demonstrates that a hyperosmolar environment can induce cell cycle arrest and cellular maturation through the regulation of NF-kappa B and WNT signaling. It provides a practical method for differentiating stem cells and suggests the use of higher osmolarity in in vitro cultures to mimic natural conditions.
Article
Biochemistry & Molecular Biology
Rocio Jimenez-Guerrero, Alejandro Belmonte-Fernandez, M. Luz Flores, Monica Gonzalez-Moreno, Begona Perez-Valderrama, Francisco Romero, Miguel A. Japon, Carmen Saez
Summary: This study reveals the role of the Wnt/β-catenin pathway in paclitaxel resistance in bladder cancer cells with CSC-like properties.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Urology & Nephrology
Theo van der Kwast, Fredrik Liedberg, Peter C. Black, Ashish Kamat, Bas W. G. van Rhijn, Ferran Algaba, David M. Berman, Arndt Hartmann, Antonio Lopez-Beltran, Hemamali Samaratunga, Murali Varma, Liang Cheng
Summary: This article explores the requirements for an optimal grading system for non-muscle-invasive bladder cancer through expert opinion. It suggests splitting the WHO 2004 high-grade category into WHO 1973 grade 2 and 3 subsets, providing more detailed histological criteria. These changes may lead to better treatment decisions for patients with bladder cancer.
EUROPEAN UROLOGY FOCUS
(2022)
Article
Urology & Nephrology
Peter C. Black, Nimira Alimohamed, Wassim Kassouf, John L. Gore, Kathy D. McCoy, Brad H. Nelson, Daniel D. De Carvalho, Rodney J. Ouellette, Ferg Devins, Tony Cornacchia, D. Robert Siemens, David M. Berman, Srikala S. Sridhar, Girish Kulkarni
CUAJ-CANADIAN UROLOGICAL ASSOCIATION JOURNAL
(2022)
Article
Biochemistry & Molecular Biology
Mei Wang, Chengfei Zhang, Qian Zheng, Zhijie Ma, Min Qi, Guangfu Di, Shizhang Ling, Haojun Xu, Bin Qi, Chengyun Yao, Hongping Xia, Xiaochun Jiang
Summary: Glioblastoma relies on new blood vessel formation, and RhoJ plays a significant role in regulating endothelial cell behavior and tumor angiogenesis. Targeting RhoJ may be an effective therapeutic strategy against GBM.
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
(2022)
Meeting Abstract
Oncology
Leszek Kotula, Anita Hryniewicz-Jankowska, Xiang Li, Katarzyna Augoff, Csaba Papp, Disharee Das, Sonia H. Y. Kung, Ladan Fazli, Tamara Jasmaspishvili, David Monty Berman, Fan Zhang, Baylee Porter, Aleksander Sikorski, Martin Gleave, Vladimir Kuznetsov, Gennady Bratslavsky
JOURNAL OF CLINICAL ONCOLOGY
(2022)
Article
Neurosciences
Xin-Jie Yang, Shizhang Ling, Meng-Liang Zhou, Hong-Ji Deng, Min Qi, Xi-Lin Liu, Cheng Zhen, Yun-Xiao Chen, Xi-Ran Fan, Ze-Yu Wu, Feng-Chun Ma, Jun Rong, Guang-Fu Di, Xiao-Chun Jiang
Summary: Neuronal apoptosis is a characteristic feature of secondary injury after traumatic brain injury (TBI) and this study reveals the specific signaling pathway involved, with TRPA1 upregulation and the involvement of Ca2+ and ROS leading to neuronal apoptosis. The findings suggest that attenuating TRPA1 can mitigate neuronal apoptosis and improve neuronal dysfunction.
Article
Multidisciplinary Sciences
Ami G. Sangster, Robert J. Gooding, Andrew Garven, Hamid Ghaedi, David M. Berman, Scott K. Davey
Summary: Muscle invasive bladder cancer is characterized by abundant mutations, particularly in DNA damage response and chromatin modification genes. The significance of these mutations and their interactions in the development and progression of the disease have been explored through the identification of mutually exclusive mutation patterns. The discovery of these patterns sheds light on the mechanisms underlying muscle invasive bladder cancer and may guide therapeutic development.
Article
Pathology
Chelsea L. Jackson, Lina Chen, Celine S. C. Hardy, Kevin Y. M. Ren, Kash Visram, Vanessa F. Bratti, Jeannette Johnstone, Gottfrid Sjodahl, David Robert Siemens, Robert J. Gooding, David M. Berman
Summary: Research using a three-antibody immunohistochemistry algorithm identified diagnostic and prognostic associations of basal and luminal subtypes in early-stage bladder cancer. Basal tumors were found to have higher grade and risk of progression to muscle invasion, while URO subtype showed faster recurrence compared to GU subtype, with URO-KRT5(+) tumors typically low grade and recurring slowly.
JOURNAL OF PATHOLOGY CLINICAL RESEARCH
(2022)
Article
Cell Biology
Celine S. C. Hardy, Hamid Ghaedi, Ava Slotman, Gottfrid Sjodahl, Robert J. Gooding, David M. Berman, Chelsea L. Jackson
Summary: A study classified bladder cancers into molecular subtypes and developed compact algorithms, demonstrating that simple immunohistochemistry classifiers can accurately distinguish different subtypes, providing appealing options for clinical implementation.
JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY
(2022)
Article
Biochemical Research Methods
Kirsty Milligan, Samantha J. Van Nest, Xinchen Deng, Ramie Ali-Adeeb, Phillip Shreeves, Samantha Punch, Nathalie Costie, Nils Pavey, Juanita M. Crook, David M. Berman, Alexandre G. Brolo, Julian J. Lum, Jeffrey L. Andrews, Andrew Jirasek
Summary: High-dose-rate brachytherapy (HDR-BT) is a promising alternative treatment for patients with intermediate risk prostate cancer. However, there is currently no bio-marker based method to monitor treatment response in HDR-BT, and the biochemical changes that occur in hypo-fractionated HDR-BT are not well understood. This pilot study aims to assess the effectiveness of Raman spectroscopy combined with principal component analysis and random-forest classification in identifying radiation response profiles after a single dose of 13.5 Gy.
JOURNAL OF BIOPHOTONICS
(2022)
Article
Genetics & Heredity
Joshua D. Silvertown, Connie Lisle, Laura Semenuk, Colleen Knapp, Jillann Jaynes, Doreen Berg, Nabodita Kaul, Josianne Lachapelle, Leslie Richardson, Marsha Speevak, Haya Sarras, David M. Berman, Ronald Carter, Harriet Feilotter, Timothy Feltis
Summary: This study investigated the prevalence of NTRK fusions in Canadian patients and provided guidance for NTRK testing paradigms. The results showed that NTRK fusions were present in 0.71% of patients, and clinically actionable incidental findings were also detected. The testing algorithm played an important role in screening patient samples.
MOLECULAR DIAGNOSIS & THERAPY
(2023)
Meeting Abstract
Medicine, Research & Experimental
Ava Slotman, Minqi Xu, Dan Winkowski, Celine Hardy, Lina Chen, Regan Baird, Chelsea Jackson, Robert Gooding, David Berman
LABORATORY INVESTIGATION
(2022)
Meeting Abstract
Pathology
Ava Slotman, Minqi Xu, Dan Winkowski, Celine Hardy, Lina Chen, Regan Baird, Chelsea Jackson, Robert Gooding, David Berman
Meeting Abstract
Medicine, General & Internal
Ninoshka Mendonca, Shizhang Ling, Ruth Marx, Yuqiong Wu, Jia Zhuo, Jeremy Waltson, Elizabeth D. Luczak, Mark Anderson, Peter Abadir
SOUTHERN MEDICAL JOURNAL
(2021)
Meeting Abstract
Biochemistry & Molecular Biology
Ninoshka Mendonca, Shizhang Ling, Diahida Bedja, Ruth Marx, Yuqiong Wu, Jia Zhuo, Jeremy Walston, Elizabeth Luczak, Mark Anderson, Peter Abadir
Article
Urology & Nephrology
Stephen Chenard, Chelsea Jackson, Thiago Vidotto, Lina Chen, Celine Hardy, Tamara Jamaspishvilli, David Berman, D. Robert Siemens, Madhuri Koti
Summary: This study revealed a higher abundance of immunosuppressive macrophages in tumors from female patients with non-muscle-invasive bladder cancer, which may lead to poorer clinical outcomes. The increased presence of CD163+ macrophages and CD79a+ B cells was associated with lower recurrence-free survival in both male and female patients.
EUROPEAN UROLOGY OPEN SCIENCE
(2021)