Article
Oncology
Marilena Karavyraki, Richard K. Porter
Summary: In this study, the metastatic potential of SCC-4 cells derived from human tongue was characterized and compared to pre-cancerous DOK cells and PGK. The study found that SCC-4 cells continuously synthesize and release IL-6, and the addition of a TLR2/TLR6 agonist enhances this process. The research also revealed that IL-6 contributes to anoikis resistance in an autocrine manner, and inhibiting IL-6 and TLR2 receptors can suppress this resistance in cancerous tongue cells.
Article
Biochemistry & Molecular Biology
Mingyan Xu, Junling Zhang, Xuemei Lu, Fan Liu, Songlin Shi, Xiaoling Deng
Summary: SMARCA4 is highly upregulated in oral squamous cell carcinoma (OSCC) tissues, promoting cell invasion and tumor growth. Mechanistic studies reveal that miR-199a-5p-regulated SMARCA4 promotes tumor cell invasion and metastasis through epithelial-mesenchymal transition (EMT). These findings provide insights into the role of SMARCA4 in OSCC and have important implications for therapy.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Tian Tian, Yunxin Lu, Jinfei Lin, Miao Chen, Huijuan Qiu, Wancui Zhu, Haohui Sun, Jinsheng Huang, Han Yang, Wuguo Deng
Summary: In this study, researchers found that the key enzyme carnitine O-palmitoyl transferase 1 (CPT1A) is upregulated in advanced esophageal squamous cell carcinoma (ESCC) and is associated with poor survival. Overexpression of CPT1A protects ESCC cells from apoptosis by maintaining redox homeostasis through supply of GSH and NADPH. The transcription factor ETV4 and ubiquitin enzyme RNF2 play important roles in regulating the expression of CPT1A. Disrupting CPT1A prevents NADPH supply and inhibits the anchorage-independent growth of ESCC cells and their lung metastases. These findings provide insights into the metastasis of ESCC and suggest a link between anoikis resistance and fatty acid oxidation.
Review
Biochemistry & Molecular Biology
Shang-Hung Chen, Sheng-Yen Hsiao, Kwang-Yu Chang, Jang-Yang Chang
Summary: Oral squamous cell carcinoma is a prevalent malignant disease worldwide, with Taiwan being particularly affected. Early detection is crucial, while understanding invasive features and the tumor microenvironment is essential for effective treatment strategies, especially for advanced cases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Oncology
Peng Yin, Shuanlong Cui, Xiangling Liao, Xiaoguang Yao
Summary: This study demonstrated the important role of Gal-3 in cetuximab-resistant OSCC, and the combination of Gal-3 inhibitor and cetuximab displayed a synergistic antitumor effect, inhibiting the development of cetuximab resistance in OSCC.
MOLECULAR MEDICINE REPORTS
(2021)
Article
Cell Biology
Chuang Wang, Chamila Kadigamuwa, Songlv Wu, Yijun Gao, Wuya Chen, Yangcong Gu, Shengli Wang, Xia Li
Summary: This study reveals that METTL3 is significantly elevated in arecoline-exposed oral squamous cell carcinoma (OSCC) cell lines and OSCC tissues of areca nut chewers. METTL3 promotes cell proliferation, migration, oncogenicity, and cisplatin resistance in arecoline-transformed OSCC cells through a positive autoregulation loop. Targeting METTL3 may be a potential strategy for treating OSCC patients with areca nut chewing history and receiving cisplatin treatment.
Article
Oncology
Feiya Zhu, Tianru Yang, Mianfeng Yao, Ting Shen, Changyun Fang
Summary: In this study, dysregulation of 13 out of 19 m(6)A-related genes in oral squamous cell carcinoma (OSCC) tissues was observed, with HNRNPA2B1 identified as the most prognostically important locus. Elevated expression of HNRNPA2B1 in OSCC was significantly associated with poor overall survival in patients. Functional studies revealed that silencing HNRNPA2B1 suppressed proliferation, migration, and invasion of OSCC via the EMT pathway. The study suggests that HNRNPA2B1 may promote carcinogenesis of OSCC through targeting EMT.
FRONTIERS IN ONCOLOGY
(2021)
Article
Oncology
Zongcheng Yang, Fen Liu, Zongkai Li, Nianping Liu, Xinfeng Yao, Yu Zhou, Liyu Zhang, Pan Jiang, Honghong Liu, Lingming Kong, Chuandong Lang, Xin Xu, Jihui Jia, Takahito Nakajima, Wenchao Gu, Lixin Zheng, Zhihong Zhang
Summary: In this study, abnormal expression of SMYD3 was found to be closely associated with the formation and poor prognosis of oral squamous cell carcinoma (OSCC). Functional experiments demonstrated that SMYD3 enhanced cancer cell stemness and proliferation in vitro and promoted tumor growth in vivo. Furthermore, SMYD3 was found to transactivate HMGA2 by increasing tri-methylation of histone H3 lysine 4, and the inhibition of SMYD3 showed anti-tumor effects in OSCC.
CLINICAL EPIGENETICS
(2023)
Article
Biotechnology & Applied Microbiology
Xiaotao Zheng, Fang Du, Xuepeng Gong, Ping Xu
Summary: Circ_0005320 is upregulated in oral squamous cell carcinoma (OSCC) and promotes tumor progression. Knockdown of circ_0005320 inhibits cell growth, migration, and invasion, and induces apoptosis in OSCC. The circ_0005320-miR-486-3p/miR-637 axis mediates the activation of the JAK2/STAT3 pathway.
Review
Biochemistry & Molecular Biology
Heinrich Botha, Camile S. Farah, Kendrick Koo, Nicola Cirillo, Michael McCullough, Rita Paolini, Antonio Celentano
Summary: Research shows that in oral squamous cell carcinoma, glucose transporter 1 (GLUT-1) and GLUT-3 expression levels are correlated with tumor stage, treatment resistance, and poor prognosis. Other glucose transporters (GLUTs) are relatively understudied and may have the potential to improve patient care with further analysis.
Article
Oncology
Omprakash Shriwas, Rakesh Arya, Sibasish Mohanty, Pallavi Mohapatra, Sugandh Kumar, Rachna Rath, Sandeep Rai Kaushik, Falak Pahwa, Krushna Chandra Murmu, Saroj Kumar Das Majumdar, Dillip Kumar Muduly, Anshuman Dixit, Punit Prasad, Ranjan K. Nanda, Rupesh Dash
Summary: The study identified RRBP1 as a major driver of cisplatin-resistance in oral squamous cell carcinoma, regulating YAP1 expression to mediate chemoresistance, and demonstrated that targeting RRBP1 could reverse cisplatin-induced chemoresistance.
BRITISH JOURNAL OF CANCER
(2021)
Article
Oncology
Christian Doll, Carolin Bestendonk, Kilian Kreutzer, Konrad Neumann, Anne Pohrt, Irena Trzpis, Steffen Koerdt, Steffen Dommerich, Max Heiland, Jan-Dirk Raguse, Korinna Joehrens
Summary: Although ERa expression is rare in OSCC, it is associated with a dramatic decrease in overall survival in male patients. Further studies are needed to confirm these results and evaluate the exact mechanism underlying this observation. Therefore, ERa-positive OSCC patients might benefit from an ER-based therapeutic (adjuvant) approach in the future.
Article
Biochemistry & Molecular Biology
Lin Qiu, Han Liu, Shuang Wang, Xiao-Hua Dai, Jian-Wei Shang, Xiao-Li Lian, Guan-Hua Wang, Jun Zhang
Summary: Oral squamous cell carcinoma (OSCC) is a major cause of cancer-related death worldwide, with its pathophysiology involving abnormal expression of the FKBP11 gene. Research has shown that FKBP11 promotes the progression of OSCC by regulating cell proliferation, cell cycle, and apoptosis pathways.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Oncology
Mingyan Xu, Feixiang Zhu, Qi Yin, Hao Yin, Shaobin Fang, Gongwei Luo, Jie Huang, Wenxia Huang, Fan Liu, Ming Zhong, Xiaoling Deng
Summary: Serum response factor (SRF) plays a role in regulating pro-carcinogenic genes in various cancers, but its function in oral squamous cell carcinoma (OSCC) is unclear. This study found that SRF is overexpressed in OSCC patients and is associated with tumor invasion and metastasis. Further experiments showed that SRF promotes OSCC cell migration and invasion by modulating the IDO1/Kyn-AhR signaling pathway. This study provides potential targets and biomarkers for diagnosing and treating OSCC.
Article
Biochemistry & Molecular Biology
Chia-Huei Lee, Yu-Hsin Tsao, Yui-Ping Weng, I-Ching Wang, Yao-Ping Chen, Pin-Feng Hung
Summary: The herbal medicine perilla leaf extract (PLE) has been found to inhibit the viability of oral squamous cell carcinoma (OSCC) cells. The main phenols in PLE, caffeic acid (CA) and rosmarinic acid (RA), were shown to reduce OSCC cell viability in a dose-dependent manner. In addition, chronic treatment with CA and RA increased the sensitivity of OSCC cells to the EGFR tyrosine kinase inhibitor gefitinib and effectively suppressed interleukin-1 beta secretion. These findings suggest that perilla phenols can be used as adjuvant therapies to improve the efficacy of chemotherapy and EGFR-targeted therapy in OSCC.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biotechnology & Applied Microbiology
Li Gao, Misun Kang, Martin Jinye Zhang, M. Sailani, Ryutaro Kuraji, April Martinez, Changchang Ye, Pachiyappan Kamarajan, Charles Le, Ling Zhan, Helene Range, Sunita P. Ho, Yvonne L. Kapila
NPJ BIOFILMS AND MICROBIOMES
(2020)
Article
Dentistry, Oral Surgery & Medicine
Ryutaro Kuraji, Ya-Hsin Wu, Shuichi Hashimoto, Yukihiro Miyashita, Saki Mishiro, Hiroshi Ito, Pachiyappan Kamarajan, Yvonne Kapila, Yukihiro Numabe
Summary: The study used Ca-45 as an index to investigate the dynamic changes in calcium metabolism during periodontitis progression in rats. The results showed significant alterations in Ca-45 radioactivity levels in the alveolar bone, reflecting a wide range of site-specific calcium metabolism disruptions induced by periodontitis within the alveolar bone.
JOURNAL OF PERIODONTAL RESEARCH
(2021)
Review
Cell Biology
Anthony J. Covarrubias, Rosalba Perrone, Alessia Grozio, Eric Verdin
Summary: NAD(+) is a crucial coenzyme in redox reactions and plays a pivotal role in energy metabolism. Its levels decline with age, and raising NAD(+) levels is being explored as a potential strategy to extend human lifespan and healthspan. NAD(+) influences key cellular processes and functions, including metabolic pathways, DNA repair, and cellular senescence, with implications for healthy aging and age-related diseases.
NATURE REVIEWS MOLECULAR CELL BIOLOGY
(2021)
Article
Health Care Sciences & Services
Yingxiang Huang, Dina Radenkovic, Kevin Perez, Kari Nadeau, Eric Verdin, David Furman
Summary: The study aims to help healthcare workers stratify and identify risks in COVID-19 patients by analyzing their characteristics. Features such as fever, use of immunosuppressant medications, use of mobility aids, shortness of breath, and severe fatigue were found to be most predictive of severe cases.
JOURNAL OF MEDICAL INTERNET RESEARCH
(2021)
Article
Immunology
Erin Trent Malone, Sean Ganther, Nevina Mena, Allan Radaic, Keemia Shariati, Abigail Kindberg, Christian Tafolla, Pachiyappan Kamarajan, J. Christopher Fenno, Ling Zhan, Yvonne L. Kapila
Summary: The periodontal complex, composed of the PDL, alveolar bone, and cementum, plays a key role in maintaining the homeostatic environment of the oral cavity. Dysbiosis of oral microbes may lead to periodontal disease, as seen in the case of Treponema denticola inducing pathological responses in PDL cells through novel mechanisms.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Virology
Marius Walter, Rosalba Perrone, Eric Verdin
Summary: Researchers developed a CRISPR-based gene drive system in herpesviruses and analyzed the evolution of resistance against human cytomegalovirus in cell culture experiments. They found that targeting evolutionarily conserved sequences can attenuate resistant viruses and reduce viral levels over time.
JOURNAL OF VIROLOGY
(2021)
Review
Dentistry, Oral Surgery & Medicine
Flavia Q. Pirih, Sepehr Monajemzadeh, Neelima Singh, Rachel Sheridan Sinacola, Jae Min Shin, Tsute Chen, J. Christopher Fenno, Pachiyappan Kamarajan, Alexander H. Rickard, Suncica Travan, Bruce J. Paster, Yvonne Kapila
Summary: Periodontitis is associated with metabolic syndrome, diabetes, and cardiovascular disease, with evidence suggesting that these conditions can alter the oral microbiome. Studies indicate an interrelationship between periodontitis and metabolic syndrome, where periodontitis may contribute to or worsen metabolic syndrome. Additional research is needed to fully comprehend the impact of these conditions on each other.
PERIODONTOLOGY 2000
(2021)
Review
Dentistry, Oral Surgery & Medicine
Allan Radaic, Sean Ganther, Pachiyappan Kamarajan, Jennifer Grandis, Sue S. Yom, Yvonne L. Kapila
Summary: The oral microbiome is a complex community of microorganisms in the oral cavity. Dysbiosis in the oral microbiome can lead to oral diseases and systemic diseases, including cancer. Antimicrobials, antimicrobial peptides, and probiotics may be promising tools for preventing and treating these cancers by targeting microbes and associated carcinogenesis processes.
PERIODONTOLOGY 2000
(2021)
Article
Microbiology
Sean Ganther, Allan Radaic, Erin Malone, Pachiyappan Kamarajan, Nai-Yuan Nicholas Chang, Christian Tafolla, Ling Zhan, J. Christopher Fenno, Yvonne L. Kapila
Summary: Periodontal disease is primarily driven by dysbiosis in the oral microbiome, leading to the overrepresentation of species that induce pro-inflammatory responses in the periodontium. The oral spirochete Treponema denticola is consistently found at significantly elevated levels in periodontal lesions and is associated with the activation of tissue-destructive genes, mainly through the surface-expressed protease complex dentilisin, stimulating tissue-destructive cellular processes in a TLR2/MyD88/Sp1-dependent fashion. These findings provide new insights into the molecular mechanisms underlying periodontal disease progression and could potentially lead to the development of more effective therapeutic treatments.
Article
Biotechnology & Applied Microbiology
Li Gao, Ryutaro Kuraji, Martin Jinye Zhang, April Martinez, Allan Radaic, Pachiyappan Kamarajan, Charles Le, Ling Zhan, Changchang Ye, Helene Range, M. Reza Sailani, Yvonne L. Kapila
Summary: Dysbiosis of the oral microbiome is associated with chronic periodontal disease, but realigning the microbiome can prevent the disease. Antibiotics and probiotics have been successful in modulating the microbiome and immune response in periodontal disease. This study examined the potential of nisin and a nisin-producing probiotic to treat periodontal bone loss, inflammation, and changes in the oral microbiome in a mouse model. The results showed that nisin and the probiotic significantly reduced periodontal pathogens, bone loss, and inflammation, while promoting the growth of connective tissue cells. Nisin and the probiotic also shifted the oral microbiome towards a healthier state. This research reveals a new biomedical application for nisin in the treatment of periodontitis and reparative medicine.
NPJ BIOFILMS AND MICROBIOMES
(2022)
Article
Microbiology
Allan Radaic, Hanna Brody, Fernando Contreras, Maryam Hajfathalian, Luke Lucido, Pachiyappan Kamarajan, Yvonne L. Kapila
Summary: The study found that nisin and the wild-type nisin-producing Lactococcus lactis probiotic can disrupt oral pathogenic biofilms in a peri-implantitis setting in vitro, promoting a healthier oral microbiome within these biofilms. Both treatments were able to shift the composition, relative abundance, and diversity levels of these biofilms towards healthy control levels.
Article
Cell Biology
Pierre-Paul Axisa, Tomomi M. Yoshida, Liliana E. Lucca, Herbert G. Kasler, Matthew R. Lincoln, Giang H. Pham, Dante Del Priore, Jean-Marie Carpier, Carrie L. Lucas, Eric Verdin, Tomokazu S. Sumida, David A. Hafler
Summary: Genome-wide association studies have identified numerous susceptibility loci for autoimmune diseases, particularly in genes active in immune cells. In this study, the authors focused on a specific variant of the histone deacetylase 7 (HDAC7) gene and found that it plays a protective role in immune regulation. The findings provide insights into the functional characterization of genetic susceptibility loci and offer potential targets for therapeutic interventions.
SCIENCE TRANSLATIONAL MEDICINE
(2022)
Article
Cell Biology
Marielle Margier, Chisaka Kuehnemann, Nicolas Hulo, Jazmin Morales, Prasanna Vadhana Ashok Kumaar, Cecile Cros, Helene Cannelle, Julie Charmetant, Eric Verdin, Matthias Canault, Alessia Grozio
Summary: It has been found that nicotinamide mononucleotide (NMN) can protect the heart from the toxic effects of doxorubicin (Doxo) and improve physical function. NMN increased tissue levels of NAD+ and its metabolites, improved survival and bodyweight loss, and prevented Doxo-induced cardiotoxicity in both acute and chronic studies. NMN also prevented Doxo-induced transcriptomic changes related to mitochondrial function, apoptosis, oxidative stress, inflammation, and p53 signaling in the heart.
Article
Nanoscience & Nanotechnology
Allan Radaic, Erin Malone, Pachiyappan Kamarajan, Yvonne L. Kapila
Summary: The study investigated the use of nisin-loaded solid lipid nanoparticles (SLN) as an antimicrobial and anticancer therapeutic, showing significant inhibition of oral pathogen growth, disruption of oral biofilms, and decrease in oral squamous cell carcinoma cell viability. The results indicate that SLN-Nisin likely decreases cell viability by increasing pore formation, demonstrating that nanoscale drug delivery systems are robust tools that can enhance bacteriocin properties.
JOURNAL OF BIOMEDICAL NANOTECHNOLOGY
(2022)
Article
Microbiology
Allan Radaic, Changchang Ye, Brett Parks, Li Gao, Ryutaro Kuraji, Erin Malone, Pachiyappan Kamarajan, Ling Zhan, Yvonne L. Kapila
JOURNAL OF ORAL MICROBIOLOGY
(2020)