Review
Pharmacology & Pharmacy
Xinru Kong, Jiangkang Xu, Xiaoye Yang, Yujia Zhai, Jianbo Ji, Guangxi Zhai
Summary: Cell-penetrating peptides have shown unique applications in tumor treatment, delivering drugs with enhanced efficacy and reduced toxicity through targeted delivery.
JOURNAL OF DRUG TARGETING
(2022)
Review
Chemistry, Medicinal
So Hee Nam, Joonhyuck Park, Heebeom Koo
Summary: Cell-penetrating peptides (CPPs) have been used to enhance the delivery of bioactive and therapeutic agents by increasing interaction with the cell membrane. However, their non-specific targeting limits their medical applications.
ARCHIVES OF PHARMACAL RESEARCH
(2023)
Review
Pharmacology & Pharmacy
Hidetomo Yokoo, Makoto Oba, Satoshi Uchida
Summary: This article highlights the distinctive features of CPP-based mRNA delivery systems, including improved mRNA bioavailability, selective targeting of dendritic cells, efficient antigen presentation, and effective mRNA delivery to the lungs by dry powder inhalation.
Article
Biochemistry & Molecular Biology
Tanja Sack, Piriththiv Dhavarasa, Daniel Szames, Siobhan O'Brien, Stephane Angers, Shana O. Kelley
Summary: Comparing to nuclear DNA repair mechanisms, the characterization of mtDNA repair mechanisms is difficult due to the challenge of specifically addressing mitochondrial damage. This study uses an mtDNA-damaging agent in combination with CRISPR/Cas9 screening to identify genes essential for mtDNA damage response and confirms the responsiveness of WRNIP1 to mtDNA damage for the first time. The study also reveals the mitochondrial role of WRNIP1 in innate immune signaling and nuclear genome maintenance.
ACS CHEMICAL BIOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Qicai Xiao, Xiao Dong, Fen Yang, Shizhe Zhou, Menghua Xiang, Liang Lou, Shao Q. Yao, Liqian Gao
Summary: Mitochondrion is a promising target for cancer therapy, and cell-penetrating peptides with mitochondrion-targeting ability are efficient tools for delivering therapeutics. New mitochondrion-targeting peptides (MTPs) have been synthesized, with MTP3 showing excellent mitochondrion-targeting ability and potential for drug delivery. The study validated the feasibility of MTP3 by preparing a mitochondrion-targeting prodrug that showed good cytotoxicity against tumor cells while having minimal toxicity towards normal cells.
CHEMISTRY-A EUROPEAN JOURNAL
(2021)
Review
Chemistry, Multidisciplinary
Muhammad Muzamil Khan, Nina Filipczak, Vladimir P. Torchilin
Summary: Cell penetrating peptides (CPP) have the ability to efficiently internalize across biological membranes, making them effective for delivering chemotherapeutic agents when combined with tumor targeting peptides.
JOURNAL OF CONTROLLED RELEASE
(2021)
Article
Biochemistry & Molecular Biology
Hyejin Park, Jung-Hyun Park, Min Seok Kim, Kwangmin Cho, Jae-Min Shin
Summary: Cell-penetrating peptides (CPPs) have the potential to deliver bioactive agents into cells. This study developed a deep-learning-based CPP prediction method, AiCPP, and found that short peptide sequences derived from amyloid precursor proteins are efficient new CPPs. These CPP sequences can be further optimized.
Article
Biochemistry & Molecular Biology
Nedal Darif, Kathrin Vogelsang, Elena Vorgia, David Schneider, Elena Deligianni, Sven Geibel, John Vontas, Shane Denecke
Summary: Cell penetrating peptides (CPPs) are small peptides that can deliver molecular cargo into cells. This study characterized the entry of four fluorescently tagged CPPs into insect cells and dissected midgut tissues. The results showed that CPPs can penetrate the plasma membrane of ovarian and midgut-derived lepidopteran cells through endosomal uptake and that this process can be inhibited by specific endocytosis inhibitors. The study also found differences in the quantity and mode of penetration among different CPPs, with CPP-1838 showing the highest efficiency in penetrating membranes through passive diffusion.
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
(2023)
Article
Nanoscience & Nanotechnology
Yezi Chen, Xiaoqin Luo, Yun Liu, Yunlei Zou, Shiqi Yang, Chaoqi Liu, Yun Zhao
Summary: Ultrasound nanobubbles were prepared as drug carriers for targeted delivery of programmed death ligand 1 monoclonal antibody (PD-L1 mAb) and doxorubicin (DOX). The nanobubbles showed significant synergistic antitumor effects by combining immunotherapy and chemotherapy, providing a potential strategy for hepatocellular carcinoma (HCC) treatment.
INTERNATIONAL JOURNAL OF NANOMEDICINE
(2022)
Article
Chemistry, Multidisciplinary
Hiroshi Kida, Yutaro Yamasaki, Loreto B. B. Feril Jr., Hitomi Endo, Keiji Itaka, Katsuro Tachibana
Summary: In this study, an efficient mRNA delivery vehicle was developed by optimizing lyophilization method for preserving human serum albumin-based nanobubbles (HSA-NBs), which eliminated the need for artificial stabilizers. The morphology of the lyophilized material was verified using scanning electron microscopy, and the properties of regenerated HSA-NBs were assessed using flow cytometry, nanoparticle tracking analysis, and resonance mass measurements. The functionality of the regenerated HSA-NBs was confirmed by increased expression of intracellularly transferred mRNA under ultrasound irradiation. The findings indicate the potential of lyophilized HSA-NBs as efficient imaging and drug delivery systems for medical applications.
Review
Pharmacology & Pharmacy
Alireza Shoari, Raheleh Tooyserkani, Mehdi Tahmasebi, Dennis W. P. M. Lowik
Summary: Cell-penetrating peptides (CPPs) are a class of peptides with the ability to cross cellular membranes, playing a crucial role in drug delivery and cancer treatment. Utilizing CPPs as carriers can achieve higher transduction efficacy and reduce toxicity towards normal tissues.
Article
Pharmacology & Pharmacy
Chunhong Su, XiaoJun Ren, Fang Yang, Bin Li, Hao Wu, Hui Li, Fang Nie
Summary: In this study, nanobubbles loaded with PDLIM5 siRNA were prepared, and their transfection efficiency and antagonism in drug resistance for NSCLC were investigated in combination with ultrasound irradiation. The results showed that this approach can effectively inhibit tumor cell growth and promote apoptosis.
Review
Chemistry, Multidisciplinary
Chen Zhang, Yihong Li, Xinyong Ma, Wenxin He, Chenxi Liu, Zhe Liu
Summary: Chemically functionalized gas-filled bubbles at micro/nano scale have shown great potential in disease diagnosis and treatment, with applications in biomedical imaging, drug delivery, gene transfection, and disease-oriented therapy. The use of ultrasound in conjunction with functional micro/nanobubbles has led to significant advancements in disease precision therapy and holds promise for future healthcare advancements.
SCIENCE CHINA-CHEMISTRY
(2021)
Review
Pharmacology & Pharmacy
Abiodun Ayo, Pirjo Laakkonen
Summary: The article discusses a new treatment strategy utilizing tumor-targeting peptides to deliver drugs, as well as the structure-function relationship data of different tumor-targeting peptides. The importance of improving peptide activity and conjugation strategies is also highlighted.
Article
Chemistry, Multidisciplinary
Qicai Xiao, Wei Du, Xiao Dong, Shubo Du, Sing Yee Ong, Guanghui Tang, Changyu Zhang, Fen Yang, Lin Li, Liqian Gao, Shao Q. Yao
Summary: Two new cell-penetrating, mitochondrion-targeting ligands (named Mito(Ligand)) capable of effectively tagging small-molecule drugs, native proteins, and nanomaterials have been discovered. These ligands have corresponding chemoselective conjugation chemistry and, upon successful cellular delivery, released native cargos were predominantly localized inside mitochondria. Delivery of doxorubicin to mitochondria resulted in potent cell cytotoxicity, disruption of mitochondrial membrane potential, and ultimately apoptosis, paving the way for future mitochondrion-targeted therapy.
CHEMISTRY-A EUROPEAN JOURNAL
(2021)