Article
Biotechnology & Applied Microbiology
Surabhi Mangal, Sanjay Chhibber, Vasundhara Singh, Kusum Harjai
Summary: The present study investigated the antimicrobial and antivirulence potential of guaiacol against Pseudomonas aeruginosa. The results demonstrated that guaiacol in combination with ciprofloxacin exhibited synergistic effects, reducing virulence factors and biofilm formation. Guaiacol also disrupted the bacterial membrane, leading to the release of cellular material.
JOURNAL OF APPLIED MICROBIOLOGY
(2022)
Letter
Biochemical Research Methods
Anming Xu, Xiaoxiao Zhang, Shixiang Cao, Xiaoli Zhou, Ziyi Yu, Xiujuan Qian, Jie Zhou, Weiliang Dong, Min Jiang
Summary: In this study, a novel transcription-associated fluorescence-activated droplet sorting method was developed for specific targeting of di-RL hyperproducers. Using this method, ten higher di-RL producing microorganisms were screened out, which showed significantly increased di-RL yield compared to the model strain. This study provides a rapid, reliable screening method for di-RL production and offers a way to measure di-RL yield in populations of droplets containing different environmental colonies.
ACS SYNTHETIC BIOLOGY
(2022)
Article
Chemistry, Physical
Fatma Allam, Mohamed Elnouby, Soraya A. Sabry, K. M. El-Khatib, Dalia E. El-Badan
Summary: This study isolated a local electroactive strain of Pseudomonas aeruginosa FA17 and optimized the composition of artificial wastewater media to investigate its electrochemical behavior, biofilm formation, and rhamnolipid production. Factors affecting current generation, biofilm formation, and rhamnolipid production by FA17 were identified, potentially leading to future development of power production by microbial electrochemical cells using the electroactive strain.
INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
(2021)
Article
Biochemistry & Molecular Biology
Alfonso Oliva, Scheherezade Garcia-Carrillo, Antonio Ortiz, Francisco J. Aranda, Jose A. Teruel
Summary: The interaction between dirhamnolipid biosurfactant and calcium ATPase from sarcoplasmic reticulum was studied, revealing that the surfactant fully inhibited ATP hydrolysis activity and induced conformational changes in the protein. This interaction made the protein more unstable and denatured at lower temperatures, as observed through enzyme activity and DSC studies.
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
(2021)
Article
Critical Care Medicine
Jill Zupetic, Hernan F. Penaloza, William Bain, Mei Hulver, Roberta Mettus, Peter Jorth, Yohei Doi, Jennifer Bomberger, Joseph Pilewski, Mehdi Nouraie, Janet S. Lee
Summary: The study found that elastase activity of Pseudomonas aeruginosa (PA) was associated with increased 30-day mortality in ICU patients. Mice infected with a high elastase producer showed increased lung bacterial burden and inflammatory profile compared to those infected with a low elastase producer.
Article
Infectious Diseases
Taehyeong Lim, So-Young Ham, SangJin Nam, Myoungsun Kim, Ki Yong Lee, Hee-Deung Park, Youngjoo Byun
Summary: This article reviews recent studies on small molecules targeting the RhlI/RhlR system in P. aeruginosa, discussing their in vitro and in vivo biological activities. The article focuses on structure-activity relationship studies and the outlook for next-generation anti-biofilm agents.
Article
Pharmacology & Pharmacy
Matthew S. S. Twigg, Simms A. A. Adu, Suguru Sugiyama, Roger Marchant, Ibrahim M. M. Banat
Summary: Glycolipid-type biosurfactants have shown potential as natural anticancer agents. In this study, mono-rhamnolipids produced by a recombinant strain of Pseudomonas aeruginosa were used to treat colorectal cancer cells. The mono-rhamnolipids significantly reduced the viability of the cancer cells and showed higher cytotoxicity compared to Piceatannol, another natural anticancer agent. The compounds appeared to induce necrosis in the cancer cells.
Article
Biotechnology & Applied Microbiology
James Redfern, Janine Wallace, Alex van Belkum, Magali Jaillard, Elliot Whittard, Roobinidevi Ragupathy, Joanna Verran, Peter Kelly, Mark Charles Enright
Summary: Pseudomonas aeruginosa is a common environmental microorganism that can form biofilms on indwelling device surfaces, promoting adhesion and survival. Research identified accessory genes and core-genome SNPs associated with enhanced biofilm production at 22 degrees C, including a 165 kb genomic island with heavy metal resistance genes.
Article
Engineering, Chemical
Mohd Nazren Radzuan, James Winterburn, Ibrahim Banat
Summary: The study demonstrates the successful production of rhamnolipid biosurfactant using PFAD and FAME by P. aeruginosa PAO1. The produced rhamnolipids were predominantly monorhamnolipid and dirhamnolipid, showing potential for high added value in various applications.
Article
Ophthalmology
Ye Li, YingWei Wang, ChunWei Li, DePeng Zhao, QinYuan Hu, Min Zhou, Miao Du, Jian Li, PengXia Wan
Summary: Elastase plays a critical role in corneal epithelial barrier dysfunction caused by Pseudomonas aeruginosa exoproteins by damaging tight junctions. Inhibition of elastase could protect the corneal epithelial barrier by reducing virulence and inflammation.
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2021)
Article
Biotechnology & Applied Microbiology
Feng Zhao, Bingxin Wang, Menglin Yuan, Sijia Ren
Summary: This study discovered that mono-rhamnolipid was superior to di-rhamnolipid in terms of antimicrobial activity for agricultural applications. Mono-rhamnolipid is an excellent candidate for agricultural biocontrol, and the knockout strain P. aeruginosa SG Delta rhlC is an excellent microbial cell factory for high production of mono-rhamnolipid.
MICROBIAL CELL FACTORIES
(2022)
Article
Infectious Diseases
Tomasz Bogiel, Dagmara Depka, Mateusz Rzepka, Joanna Kwiecinska-Pirog, Eugenia Gospodarek-Komkowska
Summary: The study investigated the frequency and genotype distribution of 10 virulence factor genes in 107 non-duplicated carbapenem-resistant P. aeruginosa isolates. Variations were observed in the prevalence and distribution of virulence genes, especially in terms of their carbapenemase synthesis ability and the strain origin.
Article
Green & Sustainable Science & Technology
Juan Shi, Yichao Chen, Xiaofeng Liu, Dong Li
Summary: This study isolated a novel halotolerant Pseudomonas aeruginosa M4, which can degrade oil and produce rhamnolipids. By optimizing conditions, the highest oil degradation rate and lipase activity were achieved, and the mechanism of oil degradation and bio-surfactant production process were explored.
JOURNAL OF CLEANER PRODUCTION
(2021)
Article
Biology
Steffen Geisel, Eleonora Secchi, Jan Vermant
Summary: Biofilms are bacterial communities encased by a self-produced matrix, and the channel networks formed within the biofilm play a vital role in promoting the transport of nutrients and chemicals. This study focuses on the role of the biofilm matrix adhesion in channel formation, specifically in Pseudomonas aeruginosa biofilms grown under constant flow in microfluidic channels. The formation of wrinkles and folds is triggered by a mechanical buckling instability, resulting in hollow channels that facilitate bacterial movement.
Review
Biotechnology & Applied Microbiology
Anming Xu, Xiaoxiao Zhang, Tong Wang, Fengxue Xin, Luyan Z. Ma, Jie Zhou, Weiliang Dong, Min Jiang
Summary: Pseudomonas aeruginosa is known for its excellent ecological flexibility and can survive in diverse niches. The adaptive mutants RSCVs have been frequently isolated from chronic infections, sparking interest in investigating their formation, persistence, and pathogenesis. RSCVs are believed to play a significant role in biofilm formation and high adaptability.
BIOTECHNOLOGY ADVANCES
(2021)