期刊
CANADIAN JOURNAL OF MICROBIOLOGY
卷 60, 期 6, 页码 355-361出版社
CANADIAN SCIENCE PUBLISHING, NRC RESEARCH PRESS
DOI: 10.1139/cjm-2014-0178
关键词
Escherichia coli; luxS; quorum sensing; flagella
资金
- Chinese National Science Foundation [31072136, 30771603, 31270171]
- Genetically Modified Organisms Technology Major Project of China [2014ZX08006-001B]
- Program for ChangJiang Scholars and Innovative Research Team In University PCSIRT [IRT0978]
- 948 Program from Ministry of Agriculture of the People's Republic of China [2011-G24]
- program granted for scientific innovation research of college graduate in Jangsu province [CXLX12 0937]
To investigate the effect of the luxS gene on the expression of virulence factors in Shiga-like toxin producing and verotoxin-producing Escherichia coli, the luxS gene from E. coli 107/86 (wild type, O139:H1:F18ab, Stx2e) was deleted. The successful deletion of luxS was confirmed by bioluminescence assays. The luxS deletion mutant exhibited changed flagella-related phenotypes, like impaired expression of flagella, decreased flagella motility, reduced biofilm formation, and reduced ability to induce pro-immunity response in host cells, which were restored after complementation with the intact luxS gene. The mutant strain also displayed attenuated production of Stx2e. This study provides new information to the crucial function of luxS in regulating Shiga-like toxin producing E. coli virulence.
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