Article
Biochemistry & Molecular Biology
Julia Aguilera-Herce, Concepcion Panadero-Medianero, Maria Antonia Sanchez-Romero, Roberto Balbontin, Joaquin Bernal-Bayard, Francisco Ramos-Morales
Summary: Type III secretion systems are commonly found in many Gram-negative pathogens and symbionts. Salmonella enterica possess two type III secretion systems associated with virulence, one for host cell invasion and another for intracellular niche maintenance. This study investigates the biochemical function of the SrfJ effector and its impact on mammalian host cells. The results indicate that SrfJ acts as a glucosylceramidase, altering the lipidome and transcriptome of host cells, both in epithelial cells and macrophages during Salmonella infection. Furthermore, analysis of gene expression changes suggests that SrfJ may play a role in protecting Salmonella from host immune defenses.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Eduardo Soto, Jorge E. Galan, Marfa Lara-Tejero
Summary: In this study, an extensive in vivo cross-linking strategy aided by structure modeling was used to investigate the sorting platform complex of type III secretion systems. The assembly process of this bacterial structure was mapped using identified cross-links as signatures for pairwise intersubunit interactions and systematic genetic deletions. Insights generated by this study could be used for the rational development of antivirulence strategies against medically important bacterial pathogens.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Multidisciplinary Sciences
Marc-Andre Leblanc, Morgan R. Fink, Thomas T. Perkins, Marcelo C. Sousa
Summary: The study shows that effector proteins from certain bacteria can be injected into host cells through a type III secretion system. These proteins need to be partially unfolded to pass through the narrow channel. Mechanical unfolding of the proteins revealed that they exhibit low force and high mechanical compliance, facilitating efficient secretion.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Review
Biochemistry & Molecular Biology
Julia Sanchez-Garrido, David Ruano-Gallego, Jyoti S. Choudhary, Gad Frankel
Summary: Recent studies have shown that T3SS effectors interact with each other in the host, revealing their interdependency and context-dependent essentiality, which is of great significance for understanding the pathogenesis of the pathogens.
TRENDS IN MICROBIOLOGY
(2022)
Review
Immunology
Guodong Zhou, Yuying Zhao, Qifeng Ma, Quan Li, Shifeng Wang, Huoying Shi
Summary: Salmonella is a dangerous bacterial species that can cause various diseases in humans and animals. This review discusses the mechanisms used by Salmonella to evade or manipulate the host immune defenses at different levels. The effector proteins delivered by the secretory systems play a key role in Salmonella's strategies against host defense mechanisms. Salmonella manipulates inflammatory pathways, ubiquitination, and autophagy processes to survive and replicate in the host.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Multidisciplinary Sciences
Brianna Steiert, Carolina M. Icardi, Robert Faris, Paige N. McCaslin, Parker Smith, Aloysius J. Klingelhutz, Peter M. Yau, Mary M. Weber
Summary: The centrosome is crucial for mitotic spindle assembly, chromosome segregation, and cell division. Infection by Chlamydia trachomatis (C.t.) leads to blocked cytokinesis, supernumerary centrosomes, and multipolar spindles, but the mechanisms behind these cellular abnormalities remain largely unknown. This study shows that the secreted effector protein CteG binds to CETN2 and is necessary for infection-induced centrosome amplification. These findings provide insights into C.t.-induced cellular abnormalities and its potential contribution to cancer risk.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Biotechnology & Applied Microbiology
Julie Ming Liang, Lisa Ann Burdette, Han Teng Wong, Danielle Tullman-Ercek
Summary: Protein production using bacteria is costly and inefficient. Researchers have engineered a bacterial secretion system to improve the production process by directly secreting soluble proteins into the culture media. They explored different activation methods and created strains capable of efficiently producing heterologous proteins. They also discovered a genetic relationship between T3SS regulators, hilE and hilD, further enhancing the industrial competitiveness of protein production strains.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Cell Biology
Sheila Bandyopadhyay, Xiao Zhang, Andrea Ascura, Karen L. Edelblum, Edward M. Bonder, Nan Gao
Summary: In this study, a novel host regulator of enteric Salmonella infections, CDC42EP1, was identified. It was found that CDC42EP1 interacts with SEPTIN-7 and Villin, and its localization and engagement with bacteria depends on host CDC42 and S. Typhimurium's capability of activating CDC42. CDC42EP1 is not required for S. Typhimurium's initial cellular entry but contributes to the pathogen's intracellular growth and replication.
JOURNAL OF CELLULAR PHYSIOLOGY
(2023)
Article
Plant Sciences
Safirah Tasa Nerves Ratu, Atsushi Hirata, Christian Oliver Kalaw, Michiko Yasuda, Mitsuaki Tabuchi, Shin Okazaki
Summary: Bel2-5 in Bradyrhizobium elkanii interacts with host targets via multiple domains to execute both NF-independent symbiosis and nodulation restriction in Rj4 soybean.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Nidhi Singh, Thales Kronenberger, Andrea Eipper, Felix Weichel, Mirita Franz-Wachtel, Boris Macek, Samuel Wagner
Summary: The study found that the salt bridges in the assembly of virulence-associated T3SS are not critical, but facilitate the incorporation of the next subunit. The conserved charged residues are crucial for type III-dependent secretion and play a key role in the function of T3SS.
JOURNAL OF MOLECULAR BIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Lisa Ann Burdette, Han Teng Wong, Danielle Tullman-Ercek
Summary: In this study, the effects of different components in the growth medium on protein secretion titer were investigated. It was found that the optimal combination of carbon sources, buffers, and salts significantly increased secretion titer. This work demonstrates the importance of optimizing medium composition to enhance protein secretion titer in bacteria.
MICROBIAL CELL FACTORIES
(2021)
Article
Genetics & Heredity
Sebastian A. Jerez, Nicolas Plaza, Veronica Bravo, Italo M. Urrutia, Carlos J. Blondel
Summary: Vibrio parahaemolyticus is a major cause of seafood-borne gastroenteritis worldwide. The T3SS2 allows the delivery of effector proteins into infected eukaryotic cells, playing a critical role in the colonization and pathogenicity of V. parahaemolyticus.
MICROBIAL GENOMICS
(2023)
Review
Cell Biology
Flavia Viana, Shruthi Sachidanandan Peringathara, Arshad Rizvi, Gunnar N. Schroeder
Summary: Proteases are powerful enzymes that irreversibly cleave peptide bonds to control critical cell fate decisions. Bacterial pathogens deliver protease effectors through secretion systems to manipulate host processes, with functional diversity reflecting adaptations to specific hosts. Emerging concepts like effector-triggered immunity provide insights into host defense against protease attacks.
CELLULAR MICROBIOLOGY
(2021)
Article
Immunology
Ines Serrano Pereira, Sara Vilela Pais, Vitor Borges, Maria Jose Borrego, Joao Paulo Gomes, Luis Jaime Mota
Summary: A study revealed that the CteG effector protein of Chlamydia trachomatis is involved in the lytic exit of host cells. Cells infected with wild-type C. trachomatis released more infectious chlamydiae in the culture supernatant compared to cells infected with a CteG-deficient strain. CteG also played a role in host cell cytotoxicity. Furthermore, CteG and Pgp4 were found to participate in a cascade of events leading to host cell lysis and release of infectious chlamydiae.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Review
Plant Sciences
Brian C. Mooney, Melissa Mantz, Emmanuelle Graciet, Pitter F. Huesgen
Summary: Pathogens and hosts are locked in an evolutionary arms race, with pathogen effectors targeting host proteins and host proteins evolving to sense and counteract these effectors. Type III effector proteases are common among bacterial pathogens and modify host proteins irreversibly. Studying effector proteases in plants provides insights into how pathogens evade host immune responses, and how hosts detect and defend against these effectors.
JOURNAL OF EXPERIMENTAL BOTANY
(2021)
