Article
Plant Sciences
Tingting Ji, Si Ma, Meiting Liang, Xueyun Wang, Lihong Gao, Yongqiang Tian
Summary: This study identified the optimal reference genes for cucumber under M. incognita stress and Pseudomonas treatment, providing potential insights into the interaction between M. incognita or Pseudomonas soil rhizosphere microbe and cucumber.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Multidisciplinary Sciences
Han Wang, Zong-Huan Ma, Juan Mao, Bai-Hong Chen
Summary: The study focused on the EXO70 gene family in grape, analyzing their characteristics and expression differences under stress conditions using bioinformatics. The results provided a foundation for further exploration of the resistance mechanisms and potential functions of these genes in grape breeding.
Article
Biochemistry & Molecular Biology
Kathannan Sankar, Hyung Joo Yoon, Young Bo Lee, Kyeong Yong Lee
Summary: Bumble bees are important alternative pollinators and model insects. This study identified stable reference genes for the bumble bee species and their different lines and tissues using RT-qPCR procedures and four analysis programs. The results emphasized the need to evaluate the stability of candidate reference genes for any differently designed lines and tissue conditions in bumble bee species.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
G. Ramakrishna, Anupam Singh, Parampreet Kaur, Sunishtha S. Yadav, Sandhya Sharma, Kishor Gaikwad
Summary: A study identified 38 small heat shock proteins (sHSPs) in the pigeonpea genome, which are highly induced by various abiotic stresses and may play a key role in mitigating stress responses.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Plant Sciences
Chen Qiu, Jinhui Chen, Weihuang Wu, Bojun Liao, Xueyan Zheng, Yong Li, Jing Huang, Jisen Shi, Zhaodong Hao
Summary: The COBRA gene in the rare and endangered woody plant Liriodendron chinense was found to encode a plant-specific glycosylphosphatidylinositol (GPI)-anchored protein (GAP) that plays a crucial role in cellulose deposition in the cell wall. The study identified 7 COBRA-like (COBL) genes in the plant's genome, which were divided into two subfamilies, SF I and SF II. The expression patterns of these genes showed tissue-specific expression, with LcCOBL5 highly expressed in the phloem and xylem, indicating its potential role in cellulose biosynthesis. Furthermore, three LcCOBL genes (LcCOBL3, LcCOBL4, and LcCOBL5) were found to respond to abiotic stresses such as cold, drought, and heat stress, suggesting their involvement in stress responses.
Article
Entomology
Li-Jun Cai, Li-Shuang Zheng, Yu-Ping Huang, Wei Xu, Min-Sheng You
Summary: This study identified 39 putative OBP genes from the diamondback moth genome and transcriptome sequences, and investigated their expression patterns across different developmental stages, sexes, and tissues. The results showed that most OBPs were highly expressed in adult antennae, with certain OBPs being regulated by mating activities and host plant feeding.
Article
Microbiology
Lingning Meng, Xiaoli Cao, Chuchu Li, Jia Li, Hui Xie, Jiping Shi, Mei Han, Han Shen, Chang Liu
Summary: This study tested the stability of ten classic housekeeping genes under the pressure of eight commonly used antibiotics in the laboratory. Results showed that the stability of gene expression was indeed affected by the types of antibiotics added, and the best reference gene set varied for different antibiotics. This study provides a comprehensive summary of the effects of laboratory antibiotics on the stability of housekeeping genes in P. aeruginosa, highlighting the necessity to select housekeeping genes according to the type of antibiotics used in the initial stage of experiment.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Environmental Sciences
Fatemeh Parvizi, Arash Akbarzadeh, Ahmad Farhadi, Sophie Arnaud-Haond, Mohammad Sharif Ranjbar
Summary: Some mollusks exhibit color variation in their soft tissues, with the color of their mantle tissue being related to the color and radiance of their shells and pearls. This study analyzed the expression of biomineralization-related genes in different color morphs of the pearl oyster, Pinctada persica, and found significant differences, with higher gene expression in the orange morphotype, particularly in warmer seasons. These findings provide insight into the molecular mechanisms underlying biomineralization in pearl oysters.
FRONTIERS IN MARINE SCIENCE
(2023)
Article
Plant Sciences
Nanyan Zhu, Chunhua Zhou
Summary: This study investigated the genetic basis of flower color formation in Rhododendron pulchrum by transcriptome analysis. Differentially expressed genes, including signal-transduction-related genes, anthocyanin biosynthesis genes, and transcription factors, were identified among different colored petal samples. The study found that higher expression levels of genes associated with flavonol synthase (FLS) may be key to white formation, while the formation of red color may be related to the higher expression of flavanone 4-reductase (DFR) families.
Article
Biochemistry & Molecular Biology
Xue Bai, Tao Chen, Yuan Wu, Mingyong Tang, Zeng-Fu Xu
Summary: This study identified a series of reference genes in tiger nut that can serve as internal controls for gene expression studies, providing convenience for qRT-PCR analysis. Among the various tissues, UCE2, UBL5, and Rubisco showed more stable expression patterns, with TUB4 and UCE2 identified as optimal reference genes for tuber development stages.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Agronomy
Devinder Sandhu, Andrew Pallete, Manju V. Pudussery, Kulbhushan K. Grover
Summary: The study evaluated four diverse guar genotypes under salinity conditions, revealing Matador and PI 268229 as salt-tolerant, and PI 340261 and PI 537281 as salt-sensitive. Salt-sensitive lines showed significantly higher leaf Na and Cl concentrations compared to salt-tolerant lines under salinity stress.
Article
Agriculture, Dairy & Animal Science
Kaitlyn M. Sommer, Youngsoo Lee, Sharon M. Donovan, Ryan N. Dilger
Summary: This study aims to determine if different mRNA purification methods can reduce interference caused by DSS. The results show that spin column purification is more effective than LiCl purification, but no method can completely eliminate interference. Therefore, caution should be exercised when interpreting gene expression results in animal studies using DSS-induced colitis.
JOURNAL OF ANIMAL SCIENCE
(2023)
Article
Entomology
Ying-Na Pan, Ru-Na Zhao, Di Fu, Chun Yu, Chun-Ni Pan, Wei Zhou, Wen-Long Chen
Summary: In this study, stable reference genes were screened for Eocanthecona furcellata under different experimental conditions, providing a basis for future molecular studies on this organism.
Article
Plant Sciences
Manu Maya Magar, Hui Liu, Guijun Yan
Summary: This study identified and analyzed the AP2/ERF superfamily genes in bread wheat and discovered candidate genes associated with heat stress response. The findings of this study provide important information and a foundation for heat tolerance breeding in wheat.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Michele Salemi, Federico Ridolfo, Maria Grazia Salluzzo, Francesca A. A. Schillaci, Salvatore Caniglia, Bartolo Lanuzza, Mariagiovanna Cantone, Raffaele Ferri
Summary: Bradykinesia, tremor, rigidity and postural instability are the hallmark of Parkinson's disease (PD). In this study, increased Humanin mRNA levels were found in peripheral blood mononuclear cells (PBMCs) of PD patients, which may serve as a marker for a better diagnosis of PD.
MOLECULAR BIOLOGY REPORTS
(2023)
Article
Microbiology
Adrien Biessy, Amy Novinscak, Jochen Blom, Genevieve Leger, Linda S. Thomashow, Francisco M. Cazorla, Dragana Josic, Martin Filion
ENVIRONMENTAL MICROBIOLOGY
(2019)
Article
Biotechnology & Applied Microbiology
A. Novinscak, M. Filion
JOURNAL OF APPLIED MICROBIOLOGY
(2019)
Article
Biotechnology & Applied Microbiology
Antoine Zboralski, Adrien Biessy, Marie-Claude Savoie, Amy Novinscak, Martin Filion
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2020)
Article
Biotechnology & Applied Microbiology
J. A. Jimenez, A. Novinscak, M. Filion
JOURNAL OF APPLIED MICROBIOLOGY
(2020)
Article
Plant Sciences
Tanya Arseneault, Roxane Roquigny, Amy Novinscak, Claudia Goyer, Martin Filion
PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY
(2020)
Article
Microbiology
Dominique Comeau, Amy Novinscak, David L. Joly, Martin Filion
FRONTIERS IN MICROBIOLOGY
(2020)
Article
Plant Sciences
Cindy Hudec, Amy Novinscak, Martin Filion
Summary: This study characterized the genetic and phenotypic diversity of pathogenic Streptomyces spp. causing Common scab (CS) in Prince Edward Island, Canada. Analysis revealed 14 distinct genetic groups, with varying levels of virulence. The most virulent genetic groups seemed to dominate the pathogenic Streptomyces spp. populations in each field, highlighting the importance of understanding population dynamics for managing this potato disease efficiently.
Article
Microbiology
Jesus A. Jimenez, Amy Novinscak, Martin Filion
FRONTIERS IN MICROBIOLOGY
(2020)
Article
Plant Sciences
Carole Balthazar, Gabrielle Cantin, Amy Novinscak, David L. Joly, Martin Filion
FRONTIERS IN PLANT SCIENCE
(2020)
Review
Biochemistry & Molecular Biology
Adrien Biessy, Martin Filion
Summary: Plant-beneficial Pseudomonas spp. aggressively colonize the rhizosphere and produce secondary metabolites like DAPG, which has broad-spectrum antimicrobial activity and contributes to disease suppression. This review discusses the biosynthesis, regulation, and role of phloroglucinol derivatives in Pseudomonas, including their mode of action as antibiotics, signaling molecules, and potential pathogenicity factors. The genetic and genomic diversity of DAPG-producing Pseudomonas spp. and its importance for enhancing biocontrol of plant pathogens are also explored.
Article
Microbiology
Antoine Zboralski, Adrien Biessy, Martin Filion
Summary: The distribution and functions of Type III secretion systems (T3SSs) in two important bacterial groups, nitrogen-fixing rhizobia and plant-beneficial Pseudomonas spp., are discussed. T3SSs and their effectors play important roles in legume-rhizobia symbiosis and in plant-beneficial Pseudomonas spp. The diversity of T3SSs in plant-beneficial bacteria suggests their significant roles in interkingdom interactions in the rhizosphere.
Editorial Material
Microbiology
Martin Filion
Article
Food Science & Technology
Amy Novinscak, Martin Filion
FRONTIERS IN SUSTAINABLE FOOD SYSTEMS
(2020)
Article
Microbiology
Amy Novinscak, David L. Joly, Martin Filion
MICROBIOLOGY RESOURCE ANNOUNCEMENTS
(2019)
Review
Biochemistry & Molecular Biology
Antoine Zboralski, Martin Filion
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2020)