Article
Cell Biology
Rebecca Loo-Kirana, Marjolijn Gilijamse, Jolanda Hogervorst, Ton Schoenmaker, Teun J. de Vries
Summary: The study compared the biological characteristics of human alveolar bone cells and PDL cells in terms of osteogenesis and osteoclastogenesis. Results showed that PDL cells were slightly more favorable for osteogenesis and induced approximately twice as many osteoclasts compared to alveolar bone cells. This indicates that alveolar bone has remodeling activity, although to a lesser extent than PDL cells.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Medicine, Research & Experimental
Zheng Zhang, Minghui Wang, Youli Zheng, Yanmei Dai, Jiashu Chou, Xiaowei Bian, Pengcheng Wang, Changyi Li, Jing Shen
Summary: This study found that miR-223 is upregulated in periodontitis and downregulated during the osteogenesis of PDL-derived cells. Overexpression of miR-223 inhibits osteogenic differentiation of PDL-derived cells, potentially by targeting the growth factor receptor genes FGFR2 and TGFβR2.
JOURNAL OF TRANSLATIONAL MEDICINE
(2022)
Article
Engineering, Biomedical
Chen Zong, Wannes Van Holm, Annelies Bronckaers, Zuodong Zhao, Stevan Cokic, Merve Kubra Aktan, Ana Belen Castro, Bart Van Meerbeek, Annabel Braem, Guy Willems, Maria Cadenas de Llano-Perula
Summary: The study shows that pretreatment of human periodontal ligament stem cells (hPDLSCs) with gold nanocomplexes (AuNCs) and transplantation in a collagen hydrogel scaffold can effectively protect alveolar bone from resorption and promote bone regeneration, suggesting a promising strategy for bone defect treatment.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Endocrinology & Metabolism
Wenjin Li, Jing Zheng, Yao Xu, Weiran Niu, Dong Guo, Jianing Cui, Wenjin Bian, Xiaohui Wang, Jinliang Niu
Summary: This study observed the elongation of unopposed rodent molars in a type 1 diabetes mellitus model and explored the pathological changes of periodontal ligament and alveolar bone, as well as their correlation with tooth axial movement. The results showed that tooth elongation was inhibited in type 1 diabetic mice, with increased osteoclasts and lower bone mineral density. There were also changes in the expression of osteogenesis-related genes.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Article
Immunology
Tugce Paksoy, Gulbahar Ustaoglu, Ahmet Ozer Sehirli, Revan Birke Koca Unsal, Serkan Sayiner, Kaan Orhan, Nurdan Bulbul Ayci, Sule Cetinel, Umut Aksoy, Ayliz Velioglu Ogunc
Summary: This study evaluated the impact of systemically administered bromelain on the progression of experimental periodontitis. The results showed that bromelain can improve the healing process, reduce bone resorption and oxidative stress by regulating cytokine levels.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2023)
Article
Physiology
Yukun Jiang, Yuzhe Guan, Yuanchen Lan, Shuo Chen, Tiancheng Li, Shujuan Zou, Zhiai Hu, Qingsong Ye
Summary: The Piezo1 channel plays a critical role in mediating bone formation and osteoclastic activities during orthodontic tooth movement, particularly on the tension side. Inhibition of Piezo1 function leads to reduced expression of osteogenesis-associated transcription factors and disruptions in bone remodeling processes.
FRONTIERS IN PHYSIOLOGY
(2021)
Article
Dentistry, Oral Surgery & Medicine
Ruben Aquino-Martinez, Brittany A. Eckhardt, Jennifer L. Rowsey, Daniel G. Fraser, Sundeep Khosla, Joshua N. Farr, David G. Monroe
Summary: As individuals age, osteocytes in the alveolar bone exhibit characteristics of senescence, leading to exacerbation of chronic inflammation and reduction in regeneration. This senescence process is associated with DNA damage and can potentiate inflammation caused by bacterial components.
JOURNAL OF PERIODONTOLOGY
(2021)
Article
Medicine, Research & Experimental
Lingping Tan, Zeyuan Cao, Huan Chen, Yunyi Xie, Le Yu, Chuanqiang Fu, Wei Zhao, Yan Wang
Summary: The study showed that curcumin could enhance cell proliferation and increase bone tissue regeneration ability of transplanted hPDLSCs under oxidative stress conditions. Curcumin reduced apoptosis and promoted osteogenesis of hPDLSCs through various mechanisms, suggesting its potential clinical application in tissue regeneration.
Article
Polymer Science
Te-Yang Huang, Mohsen Shahrousvand, Yu-Teng Hsu, Wen-Ta Su
Summary: The study aimed to evaluate the osteogenic differentiation effects of Dipsacus asper wall extracts (DAE) on periodontal ligament stem cells (PDLSCs), showing that DAE promoted cell osteogenesis and improved the physical properties of short fibers affecting the cells.
Article
Dentistry, Oral Surgery & Medicine
Liu Wang, Xiangfen Li, Yao Song, Lan Zhang, Ling Ye, Xuedong Zhou, Dongzhe Song, Dingming Huang
Summary: This study explored the effect of BMP9 on the osteogenic differentiation of inflammatory periodontal ligament stem cells (PDLSCs). The results showed that BMP9 promoted osteogenic differentiation of PDLSCs, but also upregulated the expression of inflammatory markers. NELL1 played an important role in this process by activating the MAPK/p38/ERK signaling pathway, reducing the expression of inflammatory genes induced by BMP9, and enhancing the osteogenic effect of the cells.
JOURNAL OF PERIODONTOLOGY
(2022)
Article
Immunology
Li Wang, Jing Mi, Bingjing Sun, Gang Yang, Shangfen Liu, Meihua Chen, Liming Yu, Jie Pan, Yuehua Liu
Summary: The study demonstrates the significant role of TRPC6 in the osteoblastic differentiation of PDLCs, with TRPC6 inhibitor blocking its function and TRPC6 activator promoting differentiation. In mouse model experiments, TRPC6 showed notable effects on bone regeneration and repair compared to TRPC6-/- mice.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Mehdi Salar Amoli, Mostafa EzEldeen, Reinhilde Jacobs, Veerle Bloemen
Summary: Significant progress has been made in the field of dentoalveolar tissue engineering, particularly with the development of bioprinting technology. However, the choice of suitable materials for bioprinting dentoalveolar tissues remains challenging due to their heterogeneity. This review provides an overview of the current state of research on materials used for dentoalveolar bioprinting, highlighting the advantages and shortcomings of current approaches and suggesting opportunities for further research.
Article
Materials Science, Biomaterials
Jingchen Xu, Yao Lin, Mi Tian, Xinyi Li, Yuanyuan Yin, Qiming Li, Ziyu Li, Jialiang Zhou, Xiaoge Jiang, Yulin Li, Song Chen
Summary: The study found that PDLSC-derived EVs play a positive role in tension-induced osteogenesis, enhancing the efficiency of orthodontic tooth movement (OTM) and promoting osteogenesis in bone cells. Additionally, mechano-sensitive miRNAs enriched in EVs may play a key role in promoting osteogenesis-related signaling pathways.
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2022)
Article
Dentistry, Oral Surgery & Medicine
Xiaochi Chang, Fengyi Zhou, Lingxue Bu, Nan Wang, Jing Deng, Shuai Wang
Summary: Hypoxia inhibits osteogenesis of PDLSCs, while Sema3A attenuates the hypoxia suppression of osteogenesis in PDLSCs.
JOURNAL OF PERIODONTAL RESEARCH
(2022)
Article
Biotechnology & Applied Microbiology
Xiongcheng Xu, Ziqin Chen, Long Xiao, Yanmei Xu, Nianqi Xiao, Weiqiu Jin, Yuling Chen, Yanfen Li, Kai Luo
Summary: This study aimed to investigate the effects of nanosilicate-incorporated PCL (PCL/LAP) nanofibrous membranes on periodontal ligament cells (PDLCs) in vitro and periodontal regeneration in vivo. The results showed that the PCL/LAP nanofibrous membrane exhibited good biocompatibility and bioactivity, enhanced the proliferation and osteogenic differentiation of PDLCs, and promoted periodontal regeneration by orchestrating the immunomodulatory effects of PDLCs. Therefore, the PCL/LAP nanofibrous membrane has the potential to be a promising biomaterial for periodontal regeneration therapy.
JOURNAL OF NANOBIOTECHNOLOGY
(2023)
Article
Dentistry, Oral Surgery & Medicine
Stephen K. Harrel, Thomas G. Wilson, Mirali Pandya, Thomas G. H. Diekwisch
JOURNAL OF PERIODONTOLOGY
(2019)
Editorial Material
Cell & Tissue Engineering
Thomas G. H. Diekwisch
STEM CELLS AND DEVELOPMENT
(2019)
Article
Cell & Tissue Engineering
Gokul Gopinathan, Deborah Foyle, Xianghong Luan, Thomas G. H. Diekwisch
STEM CELLS AND DEVELOPMENT
(2019)
Article
Nanoscience & Nanotechnology
Yunzhu Qian, Xuefeng Zhou, Feimin Zhang, Thomas G. H. Diekwisch, Xianghong Luan, Jianxin Yang
ACS APPLIED MATERIALS & INTERFACES
(2019)
Article
Materials Science, Biomaterials
Alisa Isaac, Faraz Jivan, Shangjing Xin, Jacob Hardin, Xianghong Luan, Mirali Pandya, Thomas G. H. Diekwisch, Daniel L. Alge
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2019)
Editorial Material
Biochemistry & Molecular Biology
Thomas G. H. Diekwisch
Review
Dentistry, Oral Surgery & Medicine
M. Francis, G. Gopinathan, D. Foyle, P. Fallah, M. Gonzalez, X. Luan, T. G. H. Diekwisch
JOURNAL OF DENTAL RESEARCH
(2020)
Article
Dentistry, Oral Surgery & Medicine
M. Francis, G. Gopinathan, A. Salapatas, S. Nares, M. Gonzalez, T. G. H. Diekwisch, X. Luan
JOURNAL OF DENTAL RESEARCH
(2020)
Article
Dentistry, Oral Surgery & Medicine
W. Ma, H. Lyu, M. Pandya, G. Gopinathan, X. Luan, T. G. H. Diekwisch
Summary: The nervous system plays a crucial role in controlling various aspects of organism life, with recent tissue regeneration research focusing on utilizing small molecule peptides to enhance biological responses. A study introduced the small molecule peptide galanin as a scaffold-coating agent for craniofacial tissue healing and regeneration, showing promising results in promoting cell proliferation, matrix synthesis, and mineralized tissue deposits. This suggests a potential novel role for neurogenic signaling pathways in craniofacial and periodontal regeneration.
JOURNAL OF DENTAL RESEARCH
(2021)
Article
Cell & Tissue Engineering
Mirali Pandya, Huling Lyu, Xianghong Luan, Thomas G. H. Diekwisch
Summary: The study demonstrates that coculturing cervical loop cells with dental pulp cells on tailored collagen scaffolds enriched with LRAP and early enamel matrix can result in singular, elongated, and polarized ameloblast-like cells expressing and secreting enamel proteins. Bioreactor microenvironments enriched with enamel matrix and LRAP are advantageous for the propagation of HAT-7 cells, leading to a significant increase in expression of amelogenin and ameloblastin enamel proteins. The use of microgravity culture systems and specific microenvironments along with tailored scaffolds show promise for the growth of ameloblast-like cells.
STEM CELLS AND DEVELOPMENT
(2021)
Article
Engineering, Biomedical
Huling Lyu, Xuefeng Zhou, Yunzhu Qian, Xiaohua Liu, Gokul Gopinathan, Mirali Pandya, Chunlin Qin, Xianghong Luan, Thomas G. H. Diekwisch
Summary: The use of a PLGA-PCL scaffold coated with dopamine and collagen, enriched with PFI-2, has shown potential in enhancing bone regeneration. Applied in an experimental periodontitis model, the scaffold resulted in significant repair of periodontal tissues and the formation of highly ordered regenerated cementum.
Article
Genetics & Heredity
Gokul Gopinathan, Xinmin Zhang, Xianghong Luan, Thomas G. H. Diekwisch
Summary: In this study, the epigenetic events that limit HOX gene expression in craniofacial tissues were investigated. High levels of repressive H3K27me3 marks were found on the HOX gene promoters in undifferentiated neural crest cells, while the ratio of K4/K27 marks was less repressive in differentiated odontogenic progenitors and other craniofacial tissues. The elevated levels of HOX genes, especially those from the HOXA and HOXB clusters, were found in alveolar bone cells. The induction of HOX expression in craniofacial neural crest cells resulted in decreased mineralization markers and promoting HOX gene expression in developing teeth led to increased patterning events and gene expression.
Article
Genetics & Heredity
Gokul Gopinathan, Xianghong Luan, Thomas G. H. Diekwisch
Summary: The nonmineralized state of the mammalian periodontal ligament is controlled by an epigenetic regulation of the RUNX2 and OSX key mineralization gene promoters.
Review
Developmental Biology
Gokul Gopinathan, Thomas G. H. Diekwisch
Summary: The epigenome controls all aspects of eukaryotic development by influencing the packaging of DNA and gene expression levels. This review focuses on three major epigenetic modifications, DNA methylation, histone methylation/acetylation, and chromatin remodeling, and provides examples of their effects on early development.
JOURNAL OF DEVELOPMENTAL BIOLOGY
(2022)
Article
Developmental Biology
Mirali Pandya, Gokul Gopinathan, Connie Tillberg, Jun Wang, Xianghong Luan, Thomas G. H. Diekwisch
Summary: YAP and TAZ signaling plays a crucial role in the mineralization and cell proliferation of periodontal tissues. Loss of YAP/TAZ leads to decreased bone density, downregulation of mineralization-associated genes, increased resorption markers, and increased osteoclast numbers.
JOURNAL OF DEVELOPMENTAL BIOLOGY
(2022)