Monoclonal antibody-europium conjugate-based lateral flow time-resolved fluoroimmunoassay for quantitative determination of T-2 toxin in cereals and feed

A method of lateral flow time-resolved fluoroimmunoassay (LF-TRFIA) was developed for rapid and ultrasensitive detection of T-2 toxin with TRFIA strips and an assorted portable TRFIA reader. Quantitative detection of T-2 toxin was realized by recording fluorescence intensities of the mAb-Eu(III) probes captured on the test line (T line) and control line (C line) of the assay strips. Quantitative standard curves for determining T-2 toxin in rice, maize, and feed were established by plotting T/C ratio against the logarithmic concentration of T-2 toxin with the linear range of 0.125-200 ng g(-1) for rice and maize and 0.25-200 ng g(-1) for feed. The limit of detection (LOD) was 0.09 ng g(-1) for rice and maize and 0.17 ng g(-1) for feed. The recovery of the standard spike ranged from 94.2% to 111.0%, and the coefficient of variation (CV) was less than 15%. The results obtained by LF-TRFIA within 15 minutes showed a good correlation with the LC-MS/MS results, indicating that the LF-TRFIA method was reliable and suitable for rapid testing.