Article
Microbiology
Ryota Miyazawa, So Shimoda, Keiichi Matsuda, Ryuta Tobe, Tasuke Ando, Hiroshi Yoneyama
Summary: Staphylococcus aureus is a major pathogen in humans and livestock, causing serious issues such as bovine mastitis. This study monitored cases of intramammary infection caused by S. aureus in the Miyagi Prefecture, Japan, and analyzed the strains obtained from dairy farms. The study found recurrent cases of infection and identified a Methicillin-Sensitive SA (MSSA) ST398 strain isolated from mastitis-containing cows, which is the first confirmed case in Japan. The results highlight the importance of nationwide surveillance to manage the distribution of ST398 strains in dairy farms for human and animal health.
Article
Ecology
Idris Nasir Abdullahi, Guillermo Juarez-Fernandez, Ursula Hofle, Javier Latorre-Fernandez, Teresa Cardona-Cabrera, David Minguez-Romero, Myriam Zarazaga, Carmen Lozano, Carmen Torres
Summary: This study investigated the nasotracheal carriage rates of Staphylococcus aureus in white storks in Southern Spain and characterized the genetic diversity of the isolates. It found that approximately 31% of white storks carried S. aureus, with nasal samples having a higher carriage rate (36.5%) than tracheal samples (11.9%). All isolates were susceptible to methicillin, and a small percentage (8.8%) were susceptible to all tested antibiotics. Various spa types and clonal complexes were detected, including the emergence of MSSA-CC398 and toxigenic MSSA strains in stork nestlings.
Article
Infectious Diseases
Ameni Arfaoui, Rym Ben Sallem, Rosa Fernandez-Fernandez, Paula Eguizabal, Raoudha Dziri, Idris Nasir Abdullahi, Noureddine Sayem, Salma Ben Khelifa Melki, Hadda-Imen Ouzari, Carmen Torres, Naouel Klibi
Summary: This study analyzed the antimicrobial resistant phenotypes and genotypes, as well as the virulence content, of S. aureus isolates from patients with diabetic foot infections in a Tunisian hospital. The study found a high prevalence of S. aureus in DFIs, with genetic diversity among the MSSA and MRSA isolates. This is the first report on the prevalence and molecular characterization of S. aureus from DFIs in Tunisia.
Article
Genetics & Heredity
Sabrina Di Gregorio, Jesus Vielma, Maria Sol Haim, Lucia Rago, Josefina Campos, Mihir Kekre, Monica Abrudan, Angela Famiglietti, Liliana Fernandez Canigia, Gabriela Rubinstein, Martha Helena von Specht, Melina Herrera, Carolina Aro, Marcelo Galas, Norah Balderrama Yarhui, Agnes Figueiredo, Nilton Lincopan, Miryan Falcon, Rosa Guillen, Teresa Camou, Gustavo Varela, David M. Aanensen, Silvia Argimon, Marta Mollerach, StaphNET-SA consortium
Summary: This article reports a genomic epidemiology study of Staphylococcus aureus in South America, which reveals the widespread antimicrobial resistance and genetic diversity of this pathogen in the region. The study also highlights the presence of high-risk clones and the need for continuous genomic surveillance.
MICROBIAL GENOMICS
(2023)
Article
Immunology
Hussain A. Alabdullah, Elise Overgaard, Danielle Scarbrough, Janet E. Williams, Omid Mohammad Mousa, Gary Dunn, Laura Bond, Mark A. McGuire, Juliette K. Tinker
Summary: The study assessed the efficacy of a vaccine against S. aureus infection, showing vaccinated animals did not exhibit clinical symptoms, had enhanced immune responses, and potential protective effects.
Article
Microbiology
Maged El-Ashker, Stefan Monecke, Mayada Gwida, Thoraya Saad, Adel El-Gohary, Amro Mohamed, Annett Reissig, Katrin Frankenfeld, Dominik Gary, Elke Mueller, Ralf Ehricht
Summary: This study aims to investigate the clonal diversity of Staphylococcus aureus strains in healthy dairy cattle, buffaloes, and their caretakers in the Nile Delta region of Egypt. The results reveal a high clonal diversity of multi-drug resistant S. aureus clones that colonize the animals and caretakers, indicating a potential health threat for both populations.
VETERINARY MICROBIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Karen Vanessa Munive Nunez, Anderson Clayton da Silva Abreu, Juliano Leonel Goncalves, Marcos Veiga dos Santos, Liliana de Oliveira Rocha, Nathalia Cristina Cirone Silva
Summary: This study evaluated the virulence and antimicrobial resistance genes of methicillin-susceptible Staphylococcus aureus isolated from subclinical bovine mastitis in Sao Paulo, Brazil. The most prevalent virulence genes were icaD, fib, and fnbA, while the most common antimicrobial resistance genes were blaZ, aac6'aph2', and ant4. The spa type t605 was found in all isolates.
JOURNAL OF APPLIED MICROBIOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Ramamoorthy Sivakumar, Parameswaran Sree Pranav, Madhavi Annamanedi, S. Chandrapriya, Shrikrishna Isloor, Jeyaprakash Rajendhran, Nagendra R. R. Hegde
Summary: This study conducted a comprehensive analysis of bovine mastitis-associated S. aureus isolates from India using whole genome sequencing and comparative genomic analysis. The results revealed 15 different sequence types and 5 clonal complexes. The genome of Indian mastitis-associated S. aureus strains is relatively closed. Phylogenetic analysis based on SNP variation differentiated the 41 strains into six major clades. Seventeen different antimicrobial resistance genes and 108 virulence-associated genes were identified among these isolates.
Article
Microbiology
Jabin Nesaraj, Alex Grinberg, Richard Laven, Patrick Biggs
Summary: We analyzed the genomes of 188 bovine-mastitis-causing S. aureus isolates from 65 dairy farms in New Zealand over a 17-year period. The analysis revealed a dominant clonal complex (CC1/ST1) that accounted for 75% of the isolates. This clonal complex showed host adaptation to cattle and exhibited temporal clonal stability.
VETERINARY MICROBIOLOGY
(2023)
Article
Agriculture, Multidisciplinary
Minhye Shin, Daye Mun, Hye Jin Choi, Sooah Kim, Shelley M. Payne, Younghoon Kim
Summary: Staphylococcus aureus RF122 Feo system is a major pathogen causing bovine mastitis, and the new antimicrobial agent PHT-427 efficiently inhibits this system, attenuating various virulence factors related to milk quality, enhancing bacterial antibiotic susceptibility, and showing no toxicity on animal model systems.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Review
Veterinary Sciences
Bruno Campos, Amy C. Pickering, Lis Souza Rocha, Ananda Pereira Aguilar, Mary Hellen Fabres-Klein, Tiago Antonio de Oliveira Mendes, J. Ross Fitzgerald, Andrea de Oliveira Barros Ribon
Summary: This review summarizes the research on bovine Staphylococcus aureus population genetics and molecular pathogenesis conducted worldwide in the past 16 years. It describes the diversity and geographical distribution of S. aureus associated with bovine mastitis, and explores the evolution of bovine S. aureus and the importance of host-adaptation in its emergence as a mastitis pathogen. The functional relevance of virulence determinants in the pathogenesis of bovine mastitis is also discussed.
BMC VETERINARY RESEARCH
(2022)
Article
Veterinary Sciences
Junjun Liu, Xin Wang, Chongliang Bi, Khalid Mehmood, Farah Ali, Jianhua Qin, Zhaoqing Han
Summary: This study investigated Staphylococcus aureus in mastitis bovine milk in Anhui and found its drug resistance and virulence genes. Understanding the drug resistance situation of S. aureus is of great importance for the prevention and treatment of public health concerns in this region.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Biotechnology & Applied Microbiology
Xiao-Yan Zhu, Meng-Ling Wang, Meng Cai, Xue-Mei Nan, Yi-Guang Zhao, Ben-Hai Xiong, Liang Yang
Summary: Mastitis is a common infectious disease in dairy farms that results in decreased milk production and quality. Staphylococcus aureus is one of the main bacteria causing subclinical mastitis. Exosomes play a role in various physiological and pathological functions by containing proteins, lipids, and nucleic acids.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2023)
Article
Veterinary Sciences
Xiaozhou Wang, Mingchao Liu, Na Geng, Yongzhen Du, Zhaoming Li, Xin Gao, Bo Han, Jianzhu Liu, Yongxia Liu
Summary: Cell death and inflammation are closely linked during mastitis caused by Staphylococcus aureus. Bovine mammary epithelial cells respond to S. aureus by NLRP3 inflammasome activation, leading to cell apoptosis and pyroptosis. This cell death process is associated with caspase-1 activation.
VETERINARY RESEARCH
(2022)
Article
Veterinary Sciences
Bruno Toledo-Silva, Fernando N. de Souza, Kristien Mertens, Sofie Piepers, Freddy Haesebrouck, Sarne De Vliegher
Summary: The formation of biofilms in Staphylococcus aureus strains causing subclinical mastitis in dairy cows is influenced by environmental signals and communication systems, with different staphylococci species impacting S. aureus biofilm formation and dispersion. Specifically, S. chromogenes enhances biofilm formation of an agr-positive S. aureus strain, while NAS isolates suppress biofilm dispersion of S. aureus. Additionally, the effects on biofilm formation and dispersion do not depend on the capacity of NAS to repress the agr system.
VETERINARY RESEARCH
(2021)
Article
Environmental Sciences
Mona M. Elsayed, Fatma A. Elgohary, Amira I. Zakaria, Rasha M. Elkenany, Ayman Y. EL-Khateeb
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2020)
Article
Microbiology
Mayada Gwida, Stefanie Lueth, Maged El-Ashker, Amira Zakaria, Fatma El-Gohary, Mona Elsayed, Sylvia Kleta, Sascha Al Dahouk
Article
Agriculture, Dairy & Animal Science
Samah Mosad Mosad, Fatma A. El-Gohary, Hanaa Said Ali, Hanem El-Sharkawy, Ehab Kotb Elmahallawy
Article
Agriculture, Dairy & Animal Science
Maged El-Ashker, Mayada Gwida, Stefan Monecke, Ralf Ehricht, Mona Elsayed, Fatma El-Gohary, Annett Reissig, Elke Mueller, Akinduti Paul, Etinosa O. Igbinosa, Abeni Beshiru, Sven Maurischat
TROPICAL ANIMAL HEALTH AND PRODUCTION
(2020)
Article
Infectious Diseases
Fatma A. El-Gohary, Lina Jamil M. Abdel-Hafez, Amira Zakaria, Radwa Reda Shata, Amin Tahoun, Amany El-Mleeh, Eman A. Abo Elfadl, Ehab Kotb Elmahallawy
INFECTION AND DRUG RESISTANCE
(2020)
Article
Agriculture, Dairy & Animal Science
Khaled Saad Abd Elfatah, Moshira Abas Elabasy, Faris El-khyate, Ehab Kotb Elmahallawy, Samah M. Mosad, Fatma A. El-Gohary, Walied Abdo, Ashraf Al-Brakati, Mohamed G. Seadawy, Abd Elnaby Tahoon, Abd Elgalil El-Gohary
Summary: Newcastle disease virus (NDV) is a highly contagious viral disease affecting various avian species, particularly chickens, with the sub-genotype VII.1.1 found in wild birds. Molecular and histopathological methods play crucial roles in detecting and characterizing the virus. Further research is needed to explore the epidemiology and strains of NDV in different bird species.
Review
Biochemistry & Molecular Biology
Ehab Kotb Elmahallawy, Yasser Mohamed, Walied Abdo, Fatma A. El-Gohary, Shaimaa Ahmed Awad Ali, Tokuma Yanai
Summary: The emergence of COVID-19 presents a major challenge to the world, with an urgent need for effective intervention strategies. Bioactive compounds in bee products may offer a promising approach to fight against the virus, but further research is required.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2021)
Review
Multidisciplinary Sciences
Stefan Monecke, Elke Mueller, Sascha D. Braun, Marc Armengol-Porta, Michele Bes, Samar Boswihi, Maged El-Ashker, Ines Engelmann, Darius Gawlik, Mayada Gwida, Helmut Hotzel, Rania Nassar, Annett Reissig, Antje Ruppelt-Lorz, Abiola Senok, Ali M. Somily, Edet E. Udo, Ralf Ehricht
Summary: In this study, a previously unknown MRSA lineage CC1153 from the Middle East, including strains from humans and livestock, was characterized. The CC1153 lineage carries specific genes and has been observed in multiple countries in the Middle East. The presence of fusidic acid-resistant MRSA poses a public health threat due to resistance to multiple antibiotics commonly used in both hospital and community settings.
SCIENTIFIC REPORTS
(2021)
Article
Genetics & Heredity
Josephine Gruetzke, Mayada Gwida, Carlus Deneke, Holger Brendebach, Michaela Projahn, Alexander Schattschneider, Dirk Hofreuter, Maged El-Ashker, Burkhard Malorny, Sascha Al Dahouk
Summary: Metagenomics serves as a valuable diagnostic tool for enhancing microbial food safety by enabling untargeted detection of pathogens, providing fast results without the need for primary isolation of microorganisms, and offering high discriminatory power for detailed molecular characterization of pathogens.
MICROBIAL GENOMICS
(2021)
Article
Microbiology
Maged El-Ashker, Stefan Monecke, Mayada Gwida, Thoraya Saad, Adel El-Gohary, Amro Mohamed, Annett Reissig, Katrin Frankenfeld, Dominik Gary, Elke Mueller, Ralf Ehricht
Summary: This study aims to investigate the clonal diversity of Staphylococcus aureus strains in healthy dairy cattle, buffaloes, and their caretakers in the Nile Delta region of Egypt. The results reveal a high clonal diversity of multi-drug resistant S. aureus clones that colonize the animals and caretakers, indicating a potential health threat for both populations.
VETERINARY MICROBIOLOGY
(2022)
Article
Veterinary Sciences
Ahmed Gareh, Amira A. Saleh, Samar M. Moustafa, Amin Tahoun, Roua S. Baty, Refaat M. A. Khalifa, Ahmed K. Dyab, Doaa A. Yones, Mohsen Arafa, Amer R. Abdelaziz, Fatma A. El-Gohary, Ehab Kotb Elmahallawy
Summary: Cystic echinococcosis, a major parasitic zoonosis associated with economic losses, was investigated in slaughtered camels and cattle in Assiut Governorate, Egypt. The parasite was found exclusively in camel lungs and mainly in cattle lungs, with different fertility rates in the two hosts. Factors like age and sex were associated with the occurrence of the disease in cattle, but not in camels. The study confirmed the presence of the parasite in both hosts, indicating their potential role in the transmission cycle.
FRONTIERS IN VETERINARY SCIENCE
(2021)
Article
Veterinary Sciences
Ehab Kotb Elmahallawy, Hesham A. Sadek, Dina Aboelsoued, Maha A. Aloraini, Abdulsalam A. M. Alkhaldi, Salma M. Abdel-Rahman, Hanna Y. Bakir, Mohsen I. Arafa, Ehssan Ahmed Hassan, Elzahara Elbaz, Eman A. A. Hassanen, Fatma A. El-Gohary, Ahmed Gareh
Summary: This study investigated the occurrence of Cryptosporidium in cattle and buffalo from Upper Egypt. Results showed a high infection rate in both animals, with different factors such as age, sex, and season influencing the likelihood of infection.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Veterinary Sciences
Ahmed Gareh, Dalia Hassan, Asmaa Essa, Saber Kotb, Mohammed Karmi, Abou El-Hamd H. Mohamed, Abeer Mousa Alkhaibari, Elzahara Elbaz, Nagwa M. Elhawary, Eman A. A. Hassanen, Maha S. Lokman, Fatma A. El-Gohary, Ehab Kotb Elmahallawy
Summary: This study investigated the acaricidal activity of neem seed extracts on camel ticks and found that the hexane extract was the most effective in killing the ticks. It can be used economically for controlling H. dromedarii ticks.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Agriculture, Dairy & Animal Science
Mohamed Marzok, Adel Almubarak, Zakriya Al Mohamad, Mohamed Salem, Alshimaa M. Farag, Hussam M. Ibrahim, Maged R. El-Ashker, Sabry El-khodery
Summary: This study aimed to establish reference values and repeatability for echocardiographic parameters in donkeys and found that these reference values can be helpful for diagnosing cardiovascular disorders in donkeys.
Article
Veterinary Sciences
Heba A. Ahmed, Rasha M. El Bayomi, Rehab Hamed, Rasha A. Mohsen, Fatma A. El-Gohary, Ahmed A. Hefny, Eman Elkhawaga, Hala M. N. Tolba
Summary: This study determined the prevalence and toxin types of antibiotic-resistant C. perfringens in different sources. The inhibitory effects of AgNPs on biofilm formation ability were investigated, and the genetic relatedness of isolates from various sources was determined using RAPD-PCR. The findings showed the high prevalence of multidrug-resistant C. perfringens and the potential of AgNPs in inhibiting biofilm formation.
VETERINARY SCIENCES
(2022)
