Article
Infectious Diseases
Natkamon Saenhom, Parichart Boueroy, Peechanika Chopjitt, Rujirat Hatrongjit, Anusak Kerdsin
Summary: Vancomycin-resistant enterococci (VRE) are a major concern as microorganisms with antimicrobial resistance and as a public health threat. A self-made screening broth can accurately and rapidly detect VRE, making it valuable for hospital infection control and clinical laboratories.
Article
Virology
S. Rigvava, I Kusradze, I. Tchgkonia, N. Karumidze, T. Dvalidze, M. Goderdzishvili
Summary: Enterococcus spp. is a common commensal microorganism, but some strains can cause opportunistic infections in humans. The spread of multi-resistant bacterial strains has renewed interest in phage therapy as a potential treatment for Enterococcus faecium infections.
Article
Infectious Diseases
Mette Pinholt, Sarah Mollerup, Kit Boye, Peder Worning, Barbara Juliane Holzknecht, Sanne Nygaard, Karen Leth Nielsen, Henrik Hasman, Louise Roer, Anette M. Hammerum, Henrik Westh, Kristian Schonning
Summary: During 2018-19, an increase in vanB VREfm was observed in the Capital Region of Denmark, mainly due to the introduction and transmission of the successful Cluster 1. By analyzing WGS data and designing a clone-specific PCR assay, vanB VREfm outbreak isolates in rectal screening samples can be rapidly and accurately detected.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2021)
Article
Biochemistry & Molecular Biology
Martin Vestergaard, Bolette Skive, Ilona Domraceva, Hanne Ingmer, Henrik Franzyk
Summary: This study identified 11 stable peptide/beta-peptoid hybrids with antibacterial activity against vancomycin-resistant Enterococcus strains, and found three analogues with bactericidal effects through membrane disruption. These findings provide promising starting points for further optimization studies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Infectious Diseases
Theresa Maria Wagner, Jessin Janice, Mark Schulz, Susan A. Ballard, Anders Goncalves da Silva, Geoffrey W. Coombs, Denise A. Daley, Stanley Pang, Shakeel Mowlaboccus, Tim Stinear, Kristin Hegstad, Benjamin P. Howden, Arnfinn Sundsfjord
Summary: This study identified eight potential vancomycin variable enterococci (VVE) isolates through whole-genome sequencing of Australian Enterococcus faecium (Efm) isolates. It was found that VVE could convert to a resistant phenotype under vancomycin selection, which was associated with a 44-bp deletion in the vanHAX promoter region and an increased vanA plasmid copy number.
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2023)
Article
Infectious Diseases
Jamil Muqtadir Bhatti, Syed Ali Raza, Ayesha Farooq Alam, Yameena Noman Khan, Ali Mala, Irshad Batool, F. N. U. Sameeullah
Summary: Enterococcal bacteremia has become prevalent in hospitalized patients, with increasing resistance to antibiotics. Inappropriate antibiotic use contributes to mortality, while targeted therapy and appropriate antibiotic use can reduce the risk of death.
BMC INFECTIOUS DISEASES
(2023)
Article
Infectious Diseases
Hanane Zerrouki, Sid-Ahmed Rebiahi, Linda Hadjadj, Fatmi Ahlem, Yamina Elhabiri, Tahar Sedrati, Jean-Marc Rolain, Seydina M. Diene
Summary: The study investigated the frequency and antibiotic resistance mechanisms of Enterococci strains isolated from hospitalised patients in Tlemcen university hospital in Algeria. Results revealed that E. faecium and E. faecalis strains were the main pathogens identified, with high resistance rate for most antibiotic classes.Vancomycin-resistant Enterococci (VRE) strains were mainly composed of E. faecium strains in the ICU, while resistant E. faecalis strains were predominant in surgical wards.
INFECTION GENETICS AND EVOLUTION
(2021)
Article
Medicine, General & Internal
Hanane Zerrouki, Sid-Ahmed Rebiahi, Linda Hadjadj, Jean-Marc Rolain, Seydina M. Diene
Summary: This study developed a real-time PCR assay for the simultaneous detection of vanA and vanB genes, showing 100% specificity and positivity on tested vancomycin-resistant isolates. The detection limit of vanA and vanB genes by this assay was 47 CFU/mL and 32 CFU/mL, respectively.
Article
Public, Environmental & Occupational Health
K. B. Gast, A. J. G. van Oudheusden, J. L. Murk, J. J. J. M. Stohr, A. G. Buiting, J. J. Verweij
Summary: This study investigated VRE carriers at Elisabeth-Tweesteden Hospital in 2018, identifying two outbreaks and using environmental sampling and whole-genome sequencing (WGS) to study genetic relatedness. Results showed high genetic relatedness of VRE, close correlation between environmental contamination and VRE transmission, and the potential of quantifying environmental contamination to reflect VRE transmission risk.
JOURNAL OF HOSPITAL INFECTION
(2021)
Article
Infectious Diseases
Beatriz Rizzo Paschoalini, Karen Vanessa Munive Nunez, Juliana Takahashi Maffei, Helio Langoni, Felipe Freitas Guimaraes, Clarice Gebara, Natylane Eufransino Freitas, Marcos Veiga dos Santos, Carlos Eduardo Fidelis, Roberto Kappes, Monica Correia Goncalves, Nathalia Cristina Cirone Silva
Summary: This study evaluates the antimicrobial resistance and virulence characteristics of Enterococcus spp. isolated from bovine milk, showing high resistance in E. faecium and E. faecalis. The findings highlight the potential risks and the need for control strategies in dairy farms.
Article
Microbiology
Andy Hesketh, Giselda Bucca, Colin P. Smith, Hee-Jeon Hong
Summary: Dalbavancin, vancomycin, and chlorobiphenyl-vancomycin are glycopeptide antibiotics with similar structures and modes of action, but they induce different gene expression patterns and have diverse impacts on bacterial cell wall biosynthesis and other physiological processes.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Food Science & Technology
Mina Nasiri, Shahram Hanifian
Summary: The study assessed the prevalence of enterococci in pasteurized milk samples from retail stores in Northwest Iran, finding that most isolates were identified as E. faecalis or E. faecium, carrying various virulence genes and showing high resistance to most antibiotics tested. Additionally, the isolates were able to produce biofilm, suggesting that pasteurized milk could potentially transmit multidrug-resistant virulent enterococci strains to humans.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Engineering, Environmental
Rui Gao, Miao Yu, Jingni Xie, Minghao Sui
Summary: This study comparatively investigated the efficacy of UV and UV/H2O2 in inactivating vancomycin-resistant Enterococcus faecalis (VRE) and degrading its containing vanB gene. The results showed that VRE was readily inactivated in both UV and UV/H2O2 processes. UV/H2O2 achieved synergistic degradation of intracellular vanB and inhibited bacterial regrowth. The presence of humic acid inhibited VRE inactivation and vanB degradation, but lower concentrations of humic acid slightly promoted vanB degradation in UV/H2O2 process.
JOURNAL OF WATER PROCESS ENGINEERING
(2022)
Article
Infectious Diseases
Beatriz Oliveira de Farias, Kayo Bianco, Ana Paula Alves Nascimento, Andressa Silva Goncalves de Brito, Thais Costa Moreira, Maysa Mandetta Clementino
Summary: This study investigated vanA-carrying Efm from WWTPs in Brazil and found their environmental spread. These isolates exhibited multidrug resistance gene expression and carried plasmids, with some genes classified as human pathogens. Multilocus sequence typing analysis revealed new alleles and different STs, including CC17 associated with hospital outbreaks. These findings are important for understanding the environmental dissemination of antibiotic-resistant bacteria.
MICROBIAL DRUG RESISTANCE
(2022)
Article
Chemistry, Medicinal
Brianna E. Dalesandro, Marcos M. Pires
Summary: Conventional small molecule antibiotics will continue to be the main treatment option in the foreseeable future, but a growing number of patients are not responding to them, indicating the need for nontraditional therapies. By grafting immunogenic agents onto bacterial cell surfaces, the immune system can be re-engaged to combat pathogenic bacteria effectively.
ACS INFECTIOUS DISEASES
(2021)