Permeability study of lipid-coated capsules with confocal laser scanning microscope (CLSM)

Based on our past work on the successfully fabricating giant liposomes, it is necessary to systematically study the capsules' permeability. In this paper. fluorescence recovery after photo-bleaching (FRAP) experiment wits carried out by confocal laser scanning microscope (CLSM) in order to quantity the 6-carboxyfluorescein (6-CF) molecules passing through capsules' wall. The results showed that the diffusion coefficient of the pure multilayered polyelectrolyte (PE) capsules was similar to the one of partly the lipid-coated PE capsules. The diffusion coefficient for completely coated PE capsules catalyzed selectively by phospholipase A, is similar to that of partly coated PE capsules.