Determination and Method Validation of Metamitron in Soil by RP-HPLC

A simple, rapid and economical method was developed and validated for the determination of metamitron using UV detector with RP-HPLC. Study of metamitron in soil was carried out. The compound was extracted from soil by methanol and clean-up was done on C-18 SPE column. Recovery ranged from 90.75 % to 94.05 % within 0.1-2.0 mu g g(-1) and RSD 1.80 %. Retention time was 3.8 min and limit of detection and limit of quantification were 0.001 and 0.008 mu g g(-1). The results indicated that the reported method could meet the requirement for the analysis of metamitron in trace amounts.