期刊
BULLETIN OF ENTOMOLOGICAL RESEARCH
卷 98, 期 6, 页码 599-603出版社
CAMBRIDGE UNIV PRESS
DOI: 10.1017/S0007485308005956
关键词
molecular species diagnosis; PCR-RFLP; mitochondrial DNA; Helicoverpa
类别
资金
- Melbourne International Research Scholarship (MIRS)
- Melbourne International Fee remission Scholarship (MIFRS)
- State Government of Victoria
- Max-Planck-Gesellschaft
- Australian Research Council (ARC)
The four significant pest species in the Helicoverpa genus (H. armigera, H. assulta, H. punctigera and H. zea) are morphologically similar and can only be reliably distinguished through dissection of adult genitalia. Two partial regions of the mitochondrial DNA (mtDNA), the cytochrome oxidase Subunit I (COI) and the cytochrome b (Cyt b) genes were amplified by PCR and digested with restriction endonucleases. The restriction patterns, generated by the endonucleases BstZ17I and HphI, demonstrated reliable differentiation of the four Helicoverpa pest species. This technique is fast, reliable and effective at distinguishing specimens irrespective of their life stages and offers Support to conventional taxonomic differentiation based on morphological characters.
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