Review
Cell Biology
Yash Chhabra, Christine M. M. Lee, Alexandra Franziska Mueller, Andrew J. Brooks
Summary: This review summarizes the current knowledge on the activation, regulation, and downstream signaling of the GH receptor, focusing on the activation of SRC family kinases by GH and the different degradation mechanisms that can regulate GHR availability. Further studies on this area are warranted considering its role in tumor progression.
MOLECULAR AND CELLULAR ENDOCRINOLOGY
(2021)
Review
Chemistry, Analytical
Juan Gao, Jingyi Jian, Zhengjin Jiang, Ann Van Schepdael
Summary: Tyrosine kinases have been extensively studied as drug targets due to their regulation of cellular processes. The deregulation of tyrosine kinases plays a key role in the pathophysiology of various diseases. Several techniques, including traditional binding assays and high-throughput screening methods, have been developed to discover new tyrosine kinase inhibitors. This review provides insights into the different assay methods for the exploration of novel tyrosine kinase inhibitors.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2023)
Article
Chemistry, Multidisciplinary
Cynthia Meyer, Melissa McCoy, Lianbo Li, Bruce Posner, Kenneth D. Westover
Summary: Measurements of kinase activity are crucial for drug development and understanding drug resistance mechanisms. A label-free, high-throughput mass spectrometry-based assay is described for studying individual kinase enzymology and drug discovery. This approach is adaptable to various kinase substrates and offers increased sensitivity. It has the potential for research on understudied kinases and kinase drug discovery.
CELL REPORTS PHYSICAL SCIENCE
(2023)
Article
Multidisciplinary Sciences
Wen Lu, Katarzyna M. Skrzypczynska, Arthur Weiss
Summary: Src-family kinases play different roles in T cell and B cell signaling, with their activity regulated by factors such as Csk. Csk is crucial for maintaining appropriate SFK activity levels and regulating PIP3 amounts to prevent inappropriate B cell activation.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Review
Cell Biology
Andra Fortner, Alexandra Chera, Antoanela Tanca, Octavian Bucur
Summary: C-terminal Src kinase (CSK) plays a crucial role in regulating critical cellular decisions, mainly through negative regulation of the SRC family of tyrosine kinases (SFKs) to promote apoptosis. Abnormal functioning of CSK and activation of SFKs are associated with diseases such as cancer, cardiovascular, and neurological disorders.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Review
Cell Biology
Li Chen, Xiangyi Kong, Yi Fang, Shishir Paunikar, Xiangyu Wang, James A. L. Brown, Emer Bourke, Xingrui Li, Jing Wang
Summary: Discoidin domain receptor tyrosine kinases (DDRs) are a type of receptor tyrosine kinases associated with various diseases, widely expressed in different cell types and involved in signaling pathways, closely related to the development and progression of solid tumors.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Pharmacology & Pharmacy
Maria Krchniakova, Silvia Paukovcekova, Petr Chlapek, Jakub Neradil, Jan Skoda, Renata Veselska
Summary: This study analyzed the effects of combining three TKIs and two iron-chelating agents in the treatment of pediatric solid tumors. The results showed that the combination of these drugs had synergistic and/or additive effects, inhibiting cell signaling and inducing apoptosis. The combination of lapatinib with the iron-chelating agents had the most significant effects. These findings provide a rationale for the novel strategy of combining iron-chelating agents with TKIs in the therapy of pediatric solid tumors.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Multidisciplinary Sciences
Samira Benadda, Mathilde Nugue, Despoina Koumantou, Marcelle Bens, Mariacristina De Luca, Olivier Pelle, Renato C. Monteiro, Irini Evnouchidou, Loredana Saveanu
Summary: This study investigated the involvement of endosomal signaling in the function of human receptors for Fc immunoglobulin fragments (FcRs). The results showed that Fc alpha RI was targeted directly to lysosomes, while Fc gamma RIIA and Fc gamma RI were internalized in specific endosomal compartments described by IRAP. The destabilization of Fc gamma R endosomal signaling compromised cytokine secretion and macrophage ability to kill tumor cells by ADCC.
Article
Biochemistry & Molecular Biology
Tufail Ali, Farah Anjum, Arunabh Choudhury, Alaa Shafie, Amal Adnan Ashour, Abdulraheem Almalki, Taj Mohammad, Md. Imtaiyaz Hassan
Summary: Spleen tyrosine kinase (SYK) is a non-receptor tyrosine kinase that plays a crucial role in signal transduction in different cell types. In this study, two natural compounds, Isolysergic acid and Michelanugine, were identified as potential SYK inhibitors with strong affinity and specificity for the SYK binding pocket. These compounds have the potential to be developed into promising SYK inhibitors for the treatment of autoimmune disorders, cancer, and inflammatory diseases.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2023)
Article
Biochemistry & Molecular Biology
Sandra I. Mota, Ligia Fao, Patricia Coelho, A. Cristina Rego
Summary: This study found that brief exposure to soluble A beta 1-42 oligomers (A beta O) induced H2O2-dependent Src activation, resulting in NMDAR activation, increased cytosolic and mitochondrial H2O2 levels, and mild mitochondrial fragmentation. These effects were prevented by Src inhibition. This study supports the important role of Src kinase in regulating mitochondrial and glutamatergic synapse homeostasis.
Article
Cell Biology
Ling Wu, Joanna Brzostek, Previtha Dawn Sakthi Vale, Qianru Wei, Clara K. T. Koh, June Xu Hui Ong, Liang-zhe Wu, Jia Chi Tan, Yen Leong Chua, Jiawei Yap, Yuan Song, Vivian Jia Yi Tan, Triscilla Y. Y. Tan, Junyun Lai, Paul A. MacAry, Nicholas R. J. Gascoigne
Summary: This study reveals that chimeric antigen receptor (CAR) signaling can occur independently of the SRC family kinase (SFK) LCK and instead relies on FYN and a CD28 intracellular domain within the CAR. LCK-deficient CAR-T cells exhibit stronger signaling through CAR and show improved in vivo efficacy with reduced exhaustion phenotype and enhanced induction of memory and proliferation. These findings provide insights into the initiation of both T cell receptor (TCR) and CAR signaling and have important clinical implications for improving CAR function.
CELL REPORTS MEDICINE
(2023)
Article
Multidisciplinary Sciences
Kyoungmi Kim, Daekee Lee
Summary: ERBB family members and their ligands are crucial for heart development and physiology. Specifically, ERBB3 is a critical binding partner of ERBB2, forming a complex that activates downstream signaling pathways for cell proliferation. The study showed that a mutant ERBB3 with altered binding sites led to lethality in embryos at specific developmental stages, highlighting the importance of AKT/ERK activation for proper heart development.
Article
Immunology
Christelle Harly, Stephen Paul Joyce, Charlotte Domblides, Thomas Bachelet, Vincent Pitard, Charlotte Mannat, Angela Pappalardo, Lionel Couzi, Sonia Netzer, Layal Massara, Emilie Obre, Omar Hawchar, Lydia Lartigue, Stephane Claverol, Carla Cano, Jean-Francois Moreau, Isabelle Mahouche, Isabelle Soubeyran, Rodrigue Rossignol, Benoit Viollet, Carrie R. Willcox, Fiyaz Mohammed, Benjamin E. Willcox, Benjamin Faustin, Julie Dechanet-Merville
Summary: In this study, the researchers identified EphA2 as a stress antigen recognized by human Vγ9Vδ1 TCR, with EphA2 being coordinately recognized by ephrin A to activate γδ TCR. The expression of EphA2 was found to be up-regulated in cancer cells undergoing AMPK-dependent metabolic reprogramming, and coexpression of EphA2 and active AMPK in tumors was associated with increased CD3 T cell infiltration in human colorectal cancer tissues.
SCIENCE IMMUNOLOGY
(2021)
Article
Multidisciplinary Sciences
Xinran Li, Amy Y. T. Lau, Angel S. N. Ng, Abdullah Aldehaiman, Yuan Zhou, Patrick K. S. Ng, Stefan T. Arold, Lydia W. T. Cheung
Summary: The study demonstrates that driver mutations in p85α can activate multiple kinase pathways and promote malignant growth of cancer cells. These mutations increase HER3 protein levels by inhibiting HER3 ubiquitination and degradation, leading to its heterodimerization with EGFR, HER2, and c-Met, as well as the allosteric activation of these dimerized partners.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Pharmacology & Pharmacy
Wen-Sheng Wu, Rui-Fang Chen, Chuan-Chu Cheng, Jia-Ling Wei, Chen-Fang Lin, Ren-In You, Yen-Chang Chen, Ming-Che Lee, Yen-Cheng Chen
Summary: Cholangiocarcinoma is the second most common type of cancer in the liver, with a poor prognosis. Inhibition of oncogenic molecules Hic-5, Src, AKT, and JNK, which are involved in the migration of CCA cells, could be a potential targeted therapy. Dasatinib, a Src inhibitor, and Hic-5 siRNA showed promising results in suppressing CCA cell invasion, and dasatinib also inhibited tumor progression in an animal model.
Article
Neurosciences
Simona Dedoni, Maria C. Olianas, Angela Ingianni, Pierluigi Onali
MOLECULAR NEUROBIOLOGY
(2017)
Article
Pharmacology & Pharmacy
Maria C. Olianas, Simona Dedoni, Pierluigi Onali
EUROPEAN JOURNAL OF PHARMACOLOGY
(2017)
Article
Biochemistry & Molecular Biology
Maria C. Olianas, Simona Dedoni, Pierluigi Onali
JOURNAL OF NEUROCHEMISTRY
(2017)
Article
Biochemistry & Molecular Biology
Maria C. Olianas, Simona Dedoni, Pierluigi Onali
NEUROCHEMICAL RESEARCH
(2018)
Article
Biochemistry & Molecular Biology
Simona Dedoni, Lee A. Campbell, Brandon K. Harvey, Valeria Avdoshina, Italo Mocchetti
JOURNAL OF NEUROCHEMISTRY
(2018)
Article
Biochemistry & Molecular Biology
Maria C. Olianas, Simona Dedoni, Pierluigi Onali
Article
Pharmacology & Pharmacy
Simona Dedoni, Luisa Marras, Maria C. Olianas, Angela Ingianni, Pierluigi Onali
JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS
(2019)
Article
Biochemistry & Molecular Biology
Simona Dedoni, Luisa Marras, Maria C. Olianas, Angela Ingianni, Pierluigi Onali
Summary: The study demonstrates that VPA upregulates TrkC in neuroblastoma cells by activating epigenetic mechanisms and signaling pathways, making the cells more sensitive to NT-3-induced apoptosis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Simona Dedoni, Alessandra Olianas, Barbara Manconi, Maria Collu, Barbara Tuveri, Maria Elena Vincis, Maria C. Olianas, Pierluigi Onali
Summary: HDAC inhibitors can induce the expression of p75NTR in neuroblastoma cells, which can be exploited to enhance the cytotoxic effect of a toxin-conjugated anti-p75NTR antibody. This novel therapeutic strategy showed promising anti-tumor effects in neuroblastoma cell cultures and murine xenograft models.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Simona Dedoni, Valeria Avdoshina, Chiara Camoglio, Carlotta Siddi, Walter Fratta, Maria Scherma, Paola Fadda
Summary: COVID-19, caused by SARS-CoV-2, is a global pandemic with potentially serious consequences. Neurological complications, including mental status change and smell and taste disorders, have been reported in infected patients. The presence of SARS-CoV-2 in brain tissue suggests neuroinvasion and possible damage. The virus may enter the brain through the olfactory mucosa. In addition, the infection triggers an inflammatory response even without direct infection of brain cells. ACE2, the entry receptor for SARS-CoV-2, is present in various brain regions. Mouse models with human ACE2 have been used to study the impact of SARS-CoV-2 on the central nervous system.
Article
Nutrition & Dietetics
Simona Dedoni, Maria Scherma, Chiara Camoglio, Carlotta Siddi, Walter Fratta, Paola Fadda
Summary: The expression of ALK receptor is reduced in rats with activity-based anorexia, indicating a potential involvement of ALK receptor in the pathophysiology of anorexia nervosa.
Article
Chemistry, Multidisciplinary
Pasquale Paribello, Bernardo Carpiniello, Roberto Murgia, Antonio Andrea Porcheddu, Sabrina El-Kacemi, Marco Pinna, Martina Contu, Giulia Costa, Rossella Barbarossa, Egea Sanna, Sara Carucci, Alessandro Zuddas, Paola Fadda, Simona Dedoni, Carlotta Siddi, Patrizia Congiu, Michela Figorilli, Michela Fanzecco, Monica Puligheddu, Antonella Gagliano, Federica Pinna, Maria Scherma, Mirko Manchia
Summary: Obsessive-compulsive disorder (OCD) is a common and highly disabling mental disorder. Different subgroups of OCD may have different impacts on cognition, functioning, sleep quality, and treatment response. There is a lack of evidence on the variation of brain-derived neurotrophic factor (BDNF) levels in OCD subgroups. Assessing inflammatory states, electroencephalogram, and polysomnography to characterize these subtypes has been hardly explored. The present project aims to increase accuracy in characterizing different OCD subtypes through clinical, cognitive, and sleep markers, along with other possible markers that may be biologically plausible.
APPLIED SCIENCES-BASEL
(2023)
Meeting Abstract
Biochemistry & Molecular Biology
Maria C. Olianas, Simona Dedoni, Pierluigi Onali
Meeting Abstract
Biochemistry & Molecular Biology
Pierluigi Onali, Simona Dedoni, Maria C. Olianas
Meeting Abstract
Biochemistry & Molecular Biology
Maria C. Olianas, Simona Dedoni, Pierluigi Onali