Article
Biophysics
Ting Wang, Qiong Yin, Hao yang Huang, Zhenyu Wang, Haixing Song, Xiaoming Luo
Summary: The poor drug penetration in the hypoxia area of solid tumors poses a significant challenge for intestinal tumor therapy. In this study, the researchers developed a bacteria propelled micro-robot using Escherichia coli Nissle 1917 (EcN) bacteria to target and deliver drugs to the hypoxic region of intestinal tumors.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2023)
Review
Biochemistry & Molecular Biology
Zejing Zhao, Shumin Xu, Wangyang Zhang, Danjun Wu, Gensheng Yang
Summary: This review discusses the probiotic properties of Escherichia coli NISSLE 1917 (EcN) in the treatment of inflammatory bowel disease (IBD), particularly ulcerative colitis. The mechanisms of EcN in the remission of IBD are proposed, and recent advancements on functionalized EcN are compiled to provide novel therapeutic strategies for the prevention and treatment of IBD.
Article
Medicine, Research & Experimental
Haojie Chen, Pengyu Lei, Hao Ji, Jiahui Ma, Yimeng Fang, Haiyang Yu, Jiao Du, Linkai Qu, Qinsi Yang, Li Luo, Kun Zhang, Wei Wu, Libo Jin, Da Sun
Summary: This study evaluated the safety and efficacy of Escherichia coli Nissle 1917 (EcN) ghosts as drug carriers for treating inflammatory bowel disease (IBD). The experiments showed that EcN ghosts had high adhesion and could attenuate IBD symptoms by inhibiting neutrophil chemotaxis and reactive oxygen species production. Due to the unique biological functions of EcN ghosts, they may be used as a strategy for targeted drug delivery in future IBD treatment.
Article
Biotechnology & Applied Microbiology
Dandan Wu, Xiaomei Li, Yanying Yu, Bingxue Gong, Xianxuan Zhou
Summary: The study showed that heparin stimulated the biofilm formation of EcN. Both the sulfation and chain length of heparin contributed to enhancing the biofilm formation of EcN, which could potentially promote the intestinal colonization of probiotics to combat pathogen infections.
BIOTECHNOLOGY LETTERS
(2021)
Article
Biotechnology & Applied Microbiology
Courtney Jones, Brianna G. Jarboe, Haley M. Majer, Amy T. Ma, Joris Beld
Summary: Escherichia coli Nissle 1917 is a Gram-negative bacterium used for treating inflammatory bowel diseases, with its ability to produce secondary metabolites playing a key role in its activity. The APE biosynthetic gene cluster and the interaction between acyl carrier proteins and phosphopantetheinyl transferase (PPTase) in EcN are essential for understanding its function. This knowledge is crucial for engineering secondary metabolite production hosts and targeting PPTases with new antibiotics.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Yi Ma, Wei Fu, Bin Hong, Xinfeng Wang, Shoujin Jiang, Jufang Wang
Summary: In this study, it was found that Probiotic Escherichia coli Nissle 1917 (EcN) inhibits the growth of Salmonella by producing the antimicrobial substance MccM. EcN::mcmA strain also reduced the adhesion and invasion ability of Salmonella to intestinal epithelial cells and decreased the secretion of inflammatory factors. These findings suggest that EcN::mcmA can be developed as engineered probiotics to combat pathogenic enterobacteria colonization in the gut.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Gastroenterology & Hepatology
Rui Zhao, Zimai Li, Yuqing Sun, Wei Ge, Mingyu Wang, Huaiwei Liu, Luying Xun, Yongzhen Xia
Summary: Hyperuricemia, the second most prevalent metabolic disease, can be effectively treated by introducing recombinant urate-degrading bacteria to enhance intestinal secretion, according to a study.
Review
Microbiology
Sefli Sri Wahyu Effendi, I-Son Ng
Summary: EcN is a notable probiotic used to treat Crohn's disease and irritable bowel syndrome. A comprehensive genomic database is helpful in assessing EcN's role in human health. Recent advancements in synthetic and genetic tools have made it possible to engineer EcN as a living medicine with controllable functions. Further studies are needed to fully explore the potential of EcN in disease treatment.
CRITICAL REVIEWS IN MICROBIOLOGY
(2023)
Article
Microbiology
Jun Shi, Dongxue Ma, Shanhu Gao, Fei Long, Xin Wang, Xingyu Pu, Richard D. Cannon, Ting-Li Han
Summary: This study found that outer membrane vesicles derived from Escherichia coli Nissle 1917 (EcN-OMVs) could reduce body weight, lower blood glucose levels, and increase plasma insulin in obese mice. EcN-OMVs treatment also modified the ratio of Firmicutes/Bacteroidetes in the gut, increased the abundance of short-chain fatty acid (SCFA)-producing flora, and influenced SCFA content in the intestine. Additionally, administration of EcN-OMVs resulted in increased levels of intestinal metabolites, hepatic ?-6 unsaturated fatty acids, and SCFAs. These findings suggest that EcN-OMVs may act as post-biotic agents that modulate gut-liver metabolism and ameliorate the pathophysiology of obesity and diabetes.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Gayoung Kim, Kyeong-Hun Choi, Hangeun Kim, Dae-Kyun Chung
Summary: L. plantarum K8 lysates can modulate the inflammatory response induced by LPS in THP-1 cells, reduce TNF-alpha production, alleviate inflammation, and inhibit characteristics such as cell enlargement and macrophage infiltration in mice.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Seita Chudan, Riko Ishibashi, Miyu Nishikawa, Yoshiaki Tabuchi, Yoshinori Nagai, Shinichi Ikushiro, Yukihiro Furusawa
Summary: This study found that soluble oat fibers ameliorate T cell-dependent colitis by inducing peripherally induced regulatory T cells (pTregs) and increasing colonic butyrate production. Soluble oat fibers promote the growth of butyrate-producing bacteria in the gut microbiome, increase the concentration of butyrate, and alleviate weight loss and inflammation in acute colitis.
Article
Chemistry, Multidisciplinary
Xinyuan Yang, Jiali Yang, Zihan Ye, Guizhen Zhang, Weimin Nie, Hui Cheng, Mengyun Peng, Kaixiang Zhang, Junjie Liu, Zhenzhong Zhang, Jinjin Shi
Summary: Coating probiotics with tannic acid and mucin can enhance their survival and colonization in the gastrointestinal tract, leading to potential applications in disease prevention and treatment.
Article
Multidisciplinary Sciences
Asma Amamou, Matthieu Rouland, Linda Yaker, Alexis Goichon, Charlene Guerin, Moutaz Aziz, Guillaume Savoye, Rachel Marion-Letellier
Summary: The study suggests that high salt diet may exacerbate intestinal fibrosis and contribute to worsen chronic inflammation.
SCIENTIFIC REPORTS
(2021)
Article
Biotechnology & Applied Microbiology
Shilong Hu, Mingyue Fei, Beibei Fu, Mingjing Yu, Panhong Yuan, Biao Tang, Hua Yang, Dongchang Sun
Summary: By protein and metabolic engineering, Escherichia coli Nissle 1917 (EcN) was successfully engineered to produce beta-alanine from glucose. The production of beta-alanine was increased by optimizing enzyme activity, metabolic flux, and culture medium. The engineered EcN obtained a high yield of beta-alanine in fed-batch fermentation.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Microbiology
Yasmine Rebai, Lysett Wagner, Mayssa Gnaien, Merle L. Hammer, Mario Kapitan, Maria Joanna Niemiec, Wael Mami, Amor Mosbah, Erij Messadi, Helmi Mardassi, Slavena Vylkova, Ilse D. Jacobsen, Sadri Znaidi
Summary: In this study, it was found that the probiotic strain Escherichia coli Nissle 1917 (EcN) can inhibit the growth of C. albicans and prevent its damage to intestinal cells. Through co-culture experiments and cell culture models, it was demonstrated that EcN can reduce the abundance of C. albicans and protect enterocytes from damage. This suggests that EcN may be a potential therapeutic approach against C. albicans infections.
Article
Nutrition & Dietetics
Francesca Algieri, Jose Garrido-Mesa, Teresa Vezza, Maria Jesus Rodriguez-Sojo, Maria Elena Rodriguez-Cabezas, Monica Olivares, Federico Garcia, Julio Galvez, Rocio Moron, Alba Rodriguez-Nogales
Summary: The study found that different probiotics have intestinal anti-inflammatory effects, particularly with slight differences in miRNA expression. Additionally, probiotics improved colitis-associated dysbiosis, although the specific bacterial groups affected varied among the probiotics tested.
EUROPEAN JOURNAL OF NUTRITION
(2021)
Article
Food Science & Technology
Chiara Sinisgalli, Teresa Vezza, Patricia Diez-Echave, Angela Ostuni, Immacolata Faraone, Laura Hidalgo-Garcia, Daniela Russo, Maria Francesca Armentano, Jose Garrido-Mesa, Maria Elena Rodriguez-Cabezas, Alba Rodriguez-Nogales, Luigi Milella, Julio Galvez
Summary: The extract from red sweet peppers with the best antioxidant properties, termed Capsicum annuum extract (CAE), was orally administered to mice fed a high fat diet in an experimental model. The CAE promoted weight loss, improved glucose and lipid metabolism, reduced systemic inflammation, restored gut barrier function, and modulated the intestinal microbiome, showing potential as a complementary approach for treating obesity.
MOLECULAR NUTRITION & FOOD RESEARCH
(2021)
Article
Nutrition & Dietetics
Cristina Sanchez-Gonzalez, Lorenzo Rivas-Garcia, Alba Rodriguez-Nogales, Francesca Algieri, Julio Galvez, Pilar Aranda, Maria Montes-Bayon, Juan Llopis
Summary: The study found that diabetic rats had higher inflammatory status and lower hematological parameters, but these improved after treatment with V, with higher serum iron levels. V reduced the gene expression of hepcidin in diabetic rats, improving the anemic state caused by diabetes.
Review
Nutrition & Dietetics
Maria Jesus Rodriguez-Sojo, Antonio Jesus Ruiz-Malagon, Maria Elena Rodriguez-Cabezas, Julio Galvez, Alba Rodriguez-Nogales
Summary: Probiotic microorganisms exert their health-associated activities through various mechanisms, with Limosilactobacillus fermentum CECT5716 standing out as a promising probiotic with anti-inflammatory and immunomodulatory properties. Studies have shown its potential beneficial effects on host health and future therapeutic approaches are being explored.
Review
Pharmacology & Pharmacy
Jose Garrido-Mesa, Kate Adams, Julio Galvez, Natividad Garrido-Mesa
Summary: Drug repurposing is a successful method to address the lack of cost-effective therapies in situations like the COVID-19 pandemic. Tetracyclines have shown efficacy in preclinical models of acute respiratory distress syndrome (ARDS) and have potential as an antiviral against SARS-CoV2. Despite limited scientific evidence, the multi-target pharmacology and safety profile of tetracyclines overcome limitations such as antibiotic activity and low commercial interest. Immunomodulatory tetracyclines and novel chemically modified non-antibiotic tetracyclines show therapeutic potential, and further drug repurposing studies in ARDS and severe COVID-19 are necessary.
EXPERT OPINION ON INVESTIGATIONAL DRUGS
(2022)
Article
Biochemistry & Molecular Biology
Antonio Jesus Ruiz-Malagon, Maria Jesus Rodriguez-Sojo, Laura Hidalgo-Garcia, Jose Alberto Molina-Tijeras, Federico Garcia, Ivo Pischel, Miguel Romero, Juan Duarte, Patricia Diez-Echave, Maria Elena Rodriguez-Cabezas, Alba Rodriguez-Nogales, Julio Galvez
Summary: This study evaluated the effect of Thymus serpyllum extract on high-fat diet-induced obesity in mice. The extract displayed antioxidant activity and improved obesity and metabolic abnormalities, as well as reducing systemic inflammation. Additionally, the extract was found to modulate the gut microbiota, restoring microbial richness and diversity.
Review
Nutrition & Dietetics
Elena Gonzalez-Lozano, Jorge Garcia-Garcia, Julio Galvez, Laura Hidalgo-Garcia, Alba Rodriguez-Nogales, Maria Elena Rodriguez-Cabezas, Manuel Sanchez
Summary: Lactobacillus probiotics have beneficial effects on host health and various pathological conditions, especially in improving inflammatory bowel disease (IBD) and regulating the immune system. Extracellular vesicles (EVs) derived from probiotics play a key role in these interactions and have been proposed as a safe alternative to live bacteria for vulnerable patients. This review provides an updated overview of EVs from different Lactobacillus strains and their potential therapeutic applications.
Article
Nutrition & Dietetics
Maria Jesus Rodriguez-Sojo, Jorge Garcia-Garcia, Antonio Jesus Ruiz-Malagon, Patricia Diez-Echave, Laura Hidalgo-Garcia, Jose Alberto Molina-Tijeras, Elena Gonzalez-Lozano, Laura Lopez-Escanez, Maria Elena Rodriguez-Cabezas, Maria Jose Rodriguez-Sanchez, Alba Rodriguez-Nogales, Cristina Mediavilla, Julio Galvez
Summary: Limosilactobacillus fermentum CECT5716, a probiotic strain isolated from human milk, has shown beneficial effects on different gastrointestinal disorders and immune responses. In this study, its effects on an irritable bowel syndrome (IBS) rat model were assessed, showing improvements in behavior, hypersensitivity, inflammation, intestinal barrier integrity, and gut microbiota composition. These findings suggest the potential clinical use of Limosilactobacillus fermentum CECT5716 for IBS management.
Article
Biochemistry & Molecular Biology
Jose D. D. Puentes-Pardo, Sara Moreno-SanJuan, Jorge Casado, Julia Escudero-Feliu, David Lopez-Perez, Paula Sanchez-Uceta, Paula Gonzalez-Novoa, Julio Galvez, Angel Carazo, Josefa Leon
Summary: Poly(ADP-ribose) polymerase-1 (PARP-1) is involved in various physiological processes. Elevated PARP-1 expression is found in several tumors, associated with stemness and tumorigenesis. In colorectal cancer patients, PARP-1 expression correlated with differentiation grade, with a significant association only in tumors with wild-type p53. Additionally, PARP-1 and cancer stem cell markers were positively correlated in these tumors. In vitro studies showed that PARP-1 regulates cancer stem cell phenotype depending on p53 status, with overexpression increasing marker expression and sphere forming ability in wild-type p53 cells, while reducing these features in mutated p53 cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Nutrition & Dietetics
Enrique Guillamon, Nuria Mut-Salud, Maria Jesus Rodriguez-Sojo, Antonio Jesus Ruiz-Malagon, Antonio Cuberos-Escobar, Antonio Martinez-Ferez, Alba Rodriguez-Nogales, Julio Galvez, Alberto Banos
Summary: This study demonstrates the potential anti-proliferative and anti-inflammatory effects of onion-derived metabolites PTS and PTSO on various human tumor lines. These compounds have the ability to induce apoptosis and reduce the levels of pro-inflammatory cytokines, suggesting their promising role in cancer prevention and/or treatment.
Article
Biochemistry & Molecular Biology
Jose Alberto Molina-Tijeras, Antonio Jesus Ruiz-Malagon, Laura Hidalgo-Garcia, Patricia Diez-Echave, Maria Jesus Rodriguez-Sojo, Maria de la Luz Cadiz-Gurrea, Antonio Segura-Carretero, Jose Perez del Palacio, Maria Reyes Gonzalez-Tejero, Maria Elena Rodriguez-Cabezas, Julio Galvez, Alba Rodriguez-Nogales, Teresa Vezza, Francesca Algieri
Summary: Obesity, a global public health problem, is increasing in prevalence rate. Plant-based treatments, such as Lavandula multifida extract (LME), have been gaining attention as a means to manage obesity and its comorbidities. In this study, LME was found to reduce weight gain, improve insulin sensitivity and glucose tolerance, decrease inflammation in the liver and adipose tissue, regulate gut permeability, and reduce oxidative stress. These findings suggest that LME may be a promising supplementary approach for managing obesity and its comorbidities.
Article
Biochemistry & Molecular Biology
Maria Jesus Rodriguez-Sojo, Antonio Jesus Ruiz-Malagon, Laura Hidalgo-Garcia, Jose Alberto Molina-Tijeras, Patricia Diez-Echave, Laura Lopez-Escanez, Lucrezia Rosati, Elena Gonzalez-Lozano, Laura Cenis-Cifuentes, Jorge Garcia-Garcia, Federico Garcia, Inaki Robles-Vera, Miguel Romero, Juan Duarte, Jose Luis Cenis, Antonio Abel Lozano-Perez, Julio Galvez, Maria Elena Rodriguez-Cabezas, Alba Rodriguez-Nogales
Summary: Morus alba L. leaf extract has beneficial effects on high-fat diet-induced obesity in mice by reducing weight gain, attenuating lipid accumulation, increasing glucose sensitivity, and improving obesity-associated inflammation. These effects are possibly due to the extract's antioxidant properties. Moreover, the extract also mitigates gut dysbiosis, as evidenced by the restoration of the Firmicutes/Bacteroidota ratio and the decrease in plasma lipopolysaccharide levels.
Article
Infectious Diseases
Jorge Garcia-Garcia, Patricia Diez-Echave, Maria Eugenia Yuste, Natalia Chueca, Federico Garcia, Jose Cabeza-Barrera, Emilio Fernandez-Varon, Julio Galvez, Manuel Colmenero, Maria Elena Rodriguez-Cabezas, Alba Rodriguez-Nogales, Rocio Moron
Summary: The number of ICU patients and antibiotic treatments has increased due to SARS-CoV-2 infection, leading to a rise in multi-drug resistant bacteria (MDRB) colonization. As these bacteria mainly come from gut microbiota, studying its composition is crucial. This pilot study aims to identify bacteria biomarkers for predicting MDRB colonization risk in SARS-CoV-2 patients in ICUs.
