Article
Food Science & Technology
Emad Shehata, Priscilla Day-Walsh, Lee Kellingray, Arjan Narbad, Paul A. Kroon
Summary: This study aims to determine the contribution of spontaneous and microbiota-dependent degradation of anthocyanins in the human colon. The results show that anthocyanins are completely degraded in a model of the human colon by a combination of spontaneous and microbiota-dependent processes. The degradation rate of anthocyanins is dependent on the B-ring substitution pattern and the type of sugar moiety.
MOLECULAR NUTRITION & FOOD RESEARCH
(2023)
Article
Chemistry, Medicinal
Ana L. Martinez-Lopez, Elizabeth Carvajal-Millan, Rafael Canett-Romero, Satya Prakash, Agustin Rascon-Chu, Yolanda L. Lopez-Franco, Jaime Lizardi-Mendoza, Valerie Micard
Summary: Arabinoxylans (AX) microcapsules loaded with insulin were prepared and evaluated using a simulator of the human intestinal microbial ecosystem. The microcapsules mainly degraded in the ascending colon and increased populations of beneficial bacteria while decreasing harmful bacteria. Oral delivery of the AX microcapsules in diabetic rats showed hypoglycemic effects and sustained insulin levels, indicating their potential as an oral insulin delivery system.
Article
Biochemistry & Molecular Biology
Feng Lu, Yuanyuan Li, Bing Zhou, Qing Guo, Yan Zhang
Summary: Early-life nutritional supplementation with grape polyphenol extract can enhance polyphenol absorption, intestinal microbiota, and intestinal barrier. This suggests the significance of polyphenol-rich nutrition in early life for improving health status.
Article
Endocrinology & Metabolism
Jacob Folz, Rebecca Neal Culver, Juan Montes Morales, Jessica Grembi, George Triadafilopoulos, David A. Relman, Kerwyn Casey Huang, Dari Shalon, Oliver Fiehn
Summary: This study used a non-invasive, ingestible sampling device to collect and analyze intestinal samples and stool samples from 15 healthy participants. The research found significant differences in food metabolites between stool and intestines, and the intestinal metabolome can reflect the connection between diet, host, and microbial metabolism.
Article
Environmental Sciences
Ali M. Khan, Mehdi Gharasoo, Lukas Y. Wick, Martin Thullner
Summary: The stable isotope fractionation of toluene was studied under dynamic phase exchange. The results showed strong fractionation in both the liquid and gas phases. Numerical simulation suggested that either a strong phase exchange fractionation or direct consumption of gas phase compounds by aqueous phase microorganisms could explain the observed effect.
ENVIRONMENTAL POLLUTION
(2022)
Article
Food Science & Technology
Nisreen Faizo, Chandrakala Aluganti Narasimhulu, Anna Forsman, Shibu Yooseph, Sampath Parthasarathy
Summary: The study utilized RNA sequencing to investigate the impact of exposure to peroxidized linoleic acid on cellular processes, uncovering alterations in lipid metabolic pathways, detoxification mechanisms, and suppression of oxidative phosphorylation. These findings provide insights into the effects of 13-HPODE on cellular processes and propose potential therapeutic strategies for LOOH-related pathologies.
Article
Biochemistry & Molecular Biology
Shumao Cui, Jie Jiang, Bowen Li, R. Paul Ross, Catherine Stanton, Jianxin Zhao, Hao Zhang, Bo Yang, Wei Chen
Summary: This study investigated the effects of short-term administration of different genotypes of P. pentosaceus on physiological characteristics, inflammation, and intestinal microecology in mice. Results showed that various strains of P. pentosaceus had different effects on gut microbiota and inflammation, with some strains being harmful while others beneficial. The presence of different genotypes of bacteriocin may explain the variations among strains, providing theoretical support for further exploring the probiotic effects of P. pentosaceus.
Review
Engineering, Environmental
Mi-Ling Li, Sae Yun Kwon, Brett A. Poulin, Martin Tsz-Ki Tsui, Laura C. Motta, Moonkyoung Cho
Summary: This paper reviews the progress in using mercury isotopes to understand the internal processes of mercury in biota. Mercury stable isotopes are a powerful tool for assessing mercury sources and biogeochemical processes. Understanding the internal mechanisms leading to mercury isotope change is crucial. There is still uncertainty in the understanding of the internal processes leading to mercury isotope fractionation.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2022)
Article
Environmental Sciences
Jiawen Zhou, Zhu Li, Xingchao Zhang, Huimin Yu, Longhua Wu, Fang Huang, Yongming Luo, Peter Christie
Summary: Improving phytoremediation techniques involves understanding plant uptake mechanisms and replenishing the bioavailable pool, which can be effectively explored using stable isotope methods. Stable isotope analysis revealed that replenishment of soil Fe- and Mn-oxide bound Zn boosted Zn uptake by the hyperaccumulator Sedum plumbizincicola during long-term remediation.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Chemistry, Applied
Wei Xia, Xiangjun Fang, Yuan Gao, Weijie Wu, Yanchao Han, Ruiling Liu, Hailong Yang, Hangjun Chen, Haiyan Gao
Summary: Food quality, safety, and nutrient metabolism play a key role in human health. However, contaminants and metabolic dysregulation in food can be problematic. Chromatography-mass spectrometry technology, especially when combined with stable isotopes, is widely used in food analysis and nutrient metabolism research. This study provides an overview of stable isotope technology applications in quantifying contaminant residues and nutrient metabolism, aiming to contribute to effective analysis techniques for protecting food quality and human health.
Article
Medicine, General & Internal
Ying Liang, Xing Shi, Yang Shen, Zhuoran Huang, Jian Wang, Changjun Shao, Yanan Chu, Jing Chen, Jun Yu, Yu Kang
Summary: This study revealed apparent dysbiosis in the intestinal microbiome of schizophrenia (SZ) patients, where microbial metabolism is dominated by protein fermentation and shift from carbohydrate fermentation and protein synthesis in healthy conditions. The concentration of protein catabolism products in feces showed positive correlations with the severity of psychiatric symptoms. These findings highlight the importance of nutrition care and the potential for developing microbiota-targeted therapeutics in SZ.
Article
Chemistry, Applied
Yajuan Bai, Yue Zhou, Xiang Li, Ruifen Zhang, Fei Huang, Bei Fan, Litao Tong, Fengzhong Wang, Mingwei Zhang
Summary: Longan pulp polysaccharide, known as LPIIa, is a bioactive compound that acts as a prebiotic and protects the intestinal barrier. This study found that LPIIa's molecular weight remained stable after gastrointestinal digestion and that 56.02% of it was consumed by gut microbiota during fecal fermentation. The intake of LPIIa increased the production of short-chain fatty acids and expression of G-protein-coupled receptor 41 in the colon, as well as improved the richness of beneficial bacteria in the colon content. Fecal fermented LPIIa showed better protection of the intestinal epithelial barrier compared to LPIIa alone.
Article
Chemistry, Applied
Matej Simek, Kristina Nesporova, Anna Kocurkova, Tereza Foglova, Gabriela Ambrozova, Vladimir Velebny, Lukas Kubala, Martina Hermannova
Summary: The in vivo fate of hyaluronan is influenced by its molecular weight, with low Mw hyaluronan being more efficiently eliminated while high Mw hyaluronan exhibits saturable kinetics. Hyaluronan is taken up by liver cells and metabolized into activated sugars in a similar manner regardless of its molecular weight. The use of 13C-labeling combined with LC-MS is an effective approach to studying the biological activities of hyaluronan.
CARBOHYDRATE POLYMERS
(2021)
Article
Chemistry, Analytical
Malama Chisanga, Howbeer Muhamadali, Danielle McDougall, Yun Xu, Nicholas Lockyer, Royston Goodacre
Summary: This study utilized Raman and FT-IR spectroscopy combined with SIP to measure 13C carbon incorporation rates and unravel microbial metabolism and interactions. The findings demonstrated the feasibility of identifying metabolic kinetics and interactions within microbial consortia using these techniques.
Article
Biochemistry & Molecular Biology
Mukundan Ragavan, Mengchen Li, Anthony G. Giacalone, Charles E. Wood, Maureen Keller-Wood, Matthew E. Merritt
Summary: Ovine models have been widely used for studying maternal-fetal interactions and congenital heart diseases. This study demonstrates a comprehensive assessment of heart function and metabolism in a perinatal model, with the use of [U-C-13]glucose as a tracer to study central energy metabolism. The methodology presented can be applied to investigate acute and chronic effects of hormonal signaling in future studies.
Article
Pharmacology & Pharmacy
Karin Larmene-Beld, Rommert Wijnsma, Albert Kuiper, Stefan van Berkel, Henri Robben, Katja Taxis, Henderik Frijlink
Summary: The study aimed to develop a strategy to qualify a prefillable autoclavable COP syringe as a container for multiple drug products in a hospital pharmacy setting. Different extraction studies and tests were performed on the syringes filled with various solutions, showing low levels of extractables and meeting predefined requirements for subvisible particles, sterility, and closure integrity. The 50mL syringes were found to have higher concentrations of silicon compared to the 5mL syringes.
EUROPEAN JOURNAL OF HOSPITAL PHARMACY
(2022)
Article
Chemistry, Medicinal
Anne-Sophie M. W. Bekhof, Florence P. A. M. van Hunsel, Sonja van de Koppel, Herman J. Woerdenbag
Summary: Tea tree essential oil, mainly consisting of monoterpenes, is commonly used as an antiseptic. This study reviewed safety data and analyzed cases of adverse reactions reported to the WHO. The findings suggest that oral administration of tea tree oil can lead to central nervous system depression and pneumonitis, while topical application may cause skin disorders. Properly stored tea tree oil within its expiration date is considered safe for non-serious inflammatory skin conditions, but the occurrence of adverse reactions is difficult to predict.
PHYTOTHERAPY RESEARCH
(2023)
Article
Gastroenterology & Hepatology
Antonius T. Otten, Hedwig H. van der Meulen, Maurice Steenhuis, Floris C. Loeff, Daan J. Touw, Jos G. W. Kosterink, Henderik W. Frijlink, Theo Rispens, Gerard Dijkstra, Marijn C. Visschedijk, Arno R. Bourgonje
Summary: A finger prick-based capillary blood self-sampling technique was validated for monitoring infliximab, vedolizumab, and C-reactive protein concentrations in patients with inflammatory bowel disease. The technique received substantial support from patients and demonstrated good tolerability and practicality.
INFLAMMATORY BOWEL DISEASES
(2023)
Article
Chemistry, Multidisciplinary
Rick Heida, Renate Akkerman, Paulo H. Jacob Silva, Anke J. Lakerveld, Daniel Ortiz, Chiara Bigogno, Matteo Gasbarri, Puck B. van Kasteren, Francesco Stellacci, Henderik W. Frijlink, Anke L. W. Huckriede, Wouter L. J. Hinrichs
Summary: Respiratory viruses, including RSV, pose a significant global health burden. Entry inhibitors, such as CD-MUS, have shown promise as antiviral drugs that can prevent infection in the respiratory tract. In this study, CD-MUS was shown to be well tolerated when administered to mice's respiratory tract and retained its activity against RSV after spray drying. This research sets the foundation for future in vivo studies on the efficacy of CD-MUS in animal models of RSV infection.
JOURNAL OF CONTROLLED RELEASE
(2023)
Article
Medicine, General & Internal
Antonius Timotheus Otten, V Peters, I Barth, C. L. Stevens, A. R. Bourgonje, H. W. Frijlink, H. J. M. Harmsen, A. Rehman, M. J. E. Campmans-Kuijpers, G. Dijkstra
Summary: This article describes a nutritional intervention trial on patients with Crohn's disease (CD) and their healthy household members, investigating the effects of an evidence-based anti-inflammatory diet and an ileocolonic-targeted capsule containing vitamin B2, B3, and C. The results of the study will provide valuable evidence for dietary recommendations for CD patients and physicians.
Review
Oncology
Herman J. Woerdenbag, Peter Olinga, Ellen A. Kok, Donald A. P. Brugman, Ulrike F. van Ark, Arwin S. Ramcharan, Paul W. Lebbink, Frederik J. H. Hoogwater, Daan G. Knapen, Derk Jan A. de Groot, Maarten W. Nijkamp
Summary: The use of cannabis products in oncology has generated interest, particularly among patients. However, the value of these products in cancer care and the associated risks are still a matter of debate despite the availability of research data. This review aims to provide a recommendation on the use of cannabis products in clinical oncology by assessing recent literature. The different types, characteristics, quality, and pharmacology of cannabis products are discussed. Standardization is crucial for ensuring reliable and reproducible quality. The oromucosal/sublingual route of administration is preferred over inhalation and drinking tea. Cannabinoids may interact with anticancer drugs and symptom management drugs, potentially enhancing or reducing their effects. Clinical research is needed to support the use, or refraining from use, of cannabis products in cancer patients.
Review
Pharmacology & Pharmacy
Primawan Putra Nugrahadi, Wouter L. J. Hinrichs, Henderik W. Frijlink, Christian Schoneich, Christina Avanti
Summary: In recent decades, there has been a significant increase in the use of therapeutic peptides. However, peptides are often unstable in aqueous solutions, which affects their stability and bioactivity. This literature review provides an overview of the degradation pathways and formulation strategies to stabilize therapeutic peptides in aqueous solutions. The most practical approaches to peptide stabilization are pH optimization and selecting the appropriate type of buffer, along with other strategies such as co-solvency, air exclusion, viscosity enhancement, PEGylation, and the use of polyol excipients.
Article
Pharmacology & Pharmacy
Renee S. van der Kooij, Martin Beukema, Anke L. W. Huckriede, Johan Zuidema, Rob Steendam, Henderik W. Frijlink, Wouter L. J. Hinrichs
Summary: Although vaccination is important for public health, the increase in vaccination coverage has slowed down. Single-injection vaccines could solve the problem of multiple injections. Researchers developed injectable microspheres that released bovine serum albumin (BSA) over several weeks. In vitro and in vivo studies showed successful sustained release and immunization.
Article
Pharmacology & Pharmacy
Aisha A. Ahmed, Antonius T. Otten, Bahez Gareb, Judith E. Huijmans, Anko C. Eissens, Ateequr Rehman, Gerard Dijkstra, Jos G. W. Kosterink, Henderik W. Frijlink, Reinout C. A. Schellekens
Summary: The development of capsules containing riboflavin, nicotinic acid, and ascorbic acid covered with a pH-sensitive coating (ColoVit) was described to achieve site-specific release in the ileocolon. The capsules met the required specifications and had a lag-time in drug release of 277-283 min, which meets the requirements for ileocolonic release. The production process of the ColoVit formulation was validated and reproducible, and the vitamin blend was stable during the production process and in the finished coated product. ColoVit is intended as an innovative treatment approach for beneficial microbiome modulation and optimization of gut health.
Article
Pharmacology & Pharmacy
Sohaib Mahri, Tobias Wilms, Paul Hagedorm, Marie-Julie Guichard, Kevin Vanvarenberg, Mireille Dumoulin, Henderik Frijlink, Rita Vanbever
Summary: In this study, the effects of PEGylation on the thermodynamic stability of rhDNase were investigated. PEGylation destabilized rhDNase upon denaturation and ethanol exposure, but PEG30-rhDNase remained stable enough to withstand aerosolization stresses. High aerosol output and excellent aerosol characteristics were achieved while preserving protein integrity and enzymatic activity.
EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES
(2023)
Article
Microbiology
Rick Heida, Henderik Frijlink, Wouter Hinrichs
Summary: The pulmonary route of administration plays an important role in the treatment of respiratory virus infections, as it can induce a broader immune response and improve drug bioavailability.
Article
Pharmacology & Pharmacy
Rick Heida, Paul Hagedoorn, Melle C. van Meel, Jurrie E. R. Prins, Frederike S. Simonis, Renate Akkerman, Anke L. W. Huckriede, Henderik W. Frijlink, Anne H. de Boer, Wouter L. J. Hinrichs
Summary: A challenge in developing dry powder formulations for inhalation is the poor reproducibility of administration to small laboratory animals. Current devices for delivering dry powder formulations to small rodents often fail to function optimally due to the use of the same puff of air for both powder dispersion and aerosol delivery. A novel aerosol generator was introduced, allowing the administration of a pre-generated aerosol to mice lungs, with controlled powder delivery. Although the emitted aerosol showed favorable size range for lung deposition and reproducibility, in vivo fluorescent imaging revealed significant differences in dose deposition and powder distribution among mice, suggesting inevitable biological variation in lung deposition.
Article
Pharmacology & Pharmacy
Khanh T. T. Nguyen, Daan Zillen, Franca F. M. van Heijningen, Kjeld J. C. van Bommel, Renz J. van Ee, Henderik W. Frijlink, Wouter L. J. Hinrichs
Summary: This study demonstrates the successful production of PBP tablets with smooth surfaces that can be directly coated with the ColoPulse coating, while preserving the stability of the encapsulated biopharmaceutical, BIAP.
Article
Chemistry, Medicinal
A. Marije Tjeerdsma, Florence P. A. M. van Hunsel, Sonja van de Koppel, Corine Ekhart, Annabella Vitalone, Herman J. Woerdenbag
Summary: This study aimed to evaluate adverse reactions of phytoestrogenic products through a review of adverse reaction reports. It found that black cohosh, hops, and soybean were the most commonly reported plant species to cause adverse reactions. The study also found that the use of products with assumed phytoestrogenic activity was associated with postmenopausal bleeding. This study highlights the importance of further investigation and monitoring of these products to ensure their safety and the well-being of individuals using them.
Article
Multidisciplinary Sciences
Y. A. de Reus, P. Hagedoorn, M. G. G. Sturkenboom, F. Grasmeijer, M. S. Bolhuis, I Sibum, H. A. M. Kerstjens, H. W. Frijlink, O. W. Akkerman
Summary: This study assessed the local tolerability, safety, and pharmacokinetic parameters of inhaled HCQ using a dry powder inhaler. The results showed that single doses of inhaled dry powder HCQ up to 20 mg are safe and well tolerated, without significant adverse effects.