Article
Medicine, Research & Experimental
Xiao Min, Xiaohan Zhang, Shujie Wang, Kyeong-Man Kim
Summary: This study aimed to investigate the activation mechanism of PKC beta II, focusing on the signaling cascade that regulates its phosphorylation and ubiquitination. The researchers found that G beta gamma and 14-3-3 epsilon mediate the constitutive phosphorylation of PKC beta II, which is necessary for its activation. They also discovered that inducible phosphorylation and Mdm2-mediated ubiquitination are required for PKC beta II's translocation to the plasma membrane. These findings will provide insights into the pathogenesis of PKC beta II-related disorders and contribute to their treatment.
Article
Biochemistry & Molecular Biology
Na Wang, Yu Qian, Ruixue Xia, Xinyan Zhu, Yangjie Xiong, Anqi Zhang, Changyou Guo, Yuanzheng He
Summary: This study presents the cryo-electron microscopy structures of human CD97 in complex with G13, Gq, and Gs, revealing the mechanism of CD97 activation and the determinants of G13 coupling selectivity.
CELL CHEMICAL BIOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Matthew Rosa, Timothy Noel, Matthew Harris, Graham Ladds
Summary: Adhesion G protein-coupled receptors (aGPCRs) form a subgroup within the GPCR superfamily with a unique structure and activation mechanism. Previous research has focused on aGPCR-ligand interactions to unravel the mysteries of this receptor family. Recent advancements have revealed a conserved method of aGPCR activation involving a tethered ligand within the GAIN domain.
BIOCHEMICAL SOCIETY TRANSACTIONS
(2021)
Article
Endocrinology & Metabolism
Enezi Khalid, John P. Chang
Summary: In goldfish, two isoforms of GnRH stimulate LH and GH secretion by acting on GnRHRs. G alpha q/11 was found to be an obligate driver of GnRH signaling in goldfish pituitary cells, and GRK2/3 showed differential involvement in GnRH actions on gonadotrophs and somatotrophs.
GENERAL AND COMPARATIVE ENDOCRINOLOGY
(2022)
Article
Neurosciences
Ting Du, Yan Rong, Rui Feng, Alexei Verkhratsky, Liang Peng
FRONTIERS IN CELLULAR NEUROSCIENCE
(2017)
Article
Endocrinology & Metabolism
Leif Hertz, Junnan Xu, Dan Song, Ting Du, Baoman Li, Enzhi Yan, Liang Peng
METABOLIC BRAIN DISEASE
(2015)
Article
Multidisciplinary Sciences
Humberto Mestre, Jeffrey Tithof, Ting Du, Wei Song, Weiguo Peng, Amanda M. Sweeney, Genaro Olveda, John H. Thomas, Maiken Nedergaard, Douglas H. Kelley
NATURE COMMUNICATIONS
(2018)
Article
Biology
Humberto Mestre, Lauren M. Hablitz, Anna L. R. Xavier, Weixi Feng, Wenyan Zou, Tinglin Pu, Hiromu Monai, Giridhar Murlidharan, Ruth M. Castellanos Rivera, Matthew J. Simon, Martin M. Pike, Virginia Pla, Ting Du, Benjamin T. Kress, Xiaowen Wang, Benjamin A. Plog, Alexander S. Thrane, Iben Lundgaard, Yoichiro Abe, Masato Yasui, John H. Thomas, Ming Xiao, Hajime Hirase, Aravind Asokan, Jeffrey J. Iliff, Maiken Nedergaard
Article
Chemistry, Multidisciplinary
Tuomas O. Lilius, Kim Blomqvist, Natalie L. Hauglund, Guojun Liu, Frederik Filip Staeger, Simone Baerentzen, Ting Du, Fredrik Ahlstrom, Janne T. Backman, Eija A. Kalso, Pekka Rauhala, Maiken Nedergaard
JOURNAL OF CONTROLLED RELEASE
(2019)
Article
Multidisciplinary Sciences
Amanda M. Sweeney, Virginia Pla, Ting Du, Guojun Liu, Qian Sun, Sisi Peng, Benjamin A. Plog, Benjamin T. Kress, Xiaowei Wang, Humberto Mestre, Maiken Nedergaard
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2019)
Article
Multidisciplinary Sciences
Humberto Mestre, Ting Du, Amanda M. Sweeney, Guojun Liu, Andrew J. Samson, Weiguo Peng, Kristian Nygaard Mortensen, Frederik Filip Staeger, Peter A. R. Bork, Logan Bashford, Edna R. Toro, Jeffrey Tithof, Douglas H. Kelley, John H. Thomas, Poul G. Hjorth, Erik A. Martens, Rupal I. Mehta, Orestes Solis, Pablo Blinder, David Kleinfeld, Hajime Hirase, Yuki Mori, Maiken Nedergaard
Article
Cell Biology
Guojun Liu, Humberto Mestre, Amanda M. Sweeney, Qian Sun, Pia Weikop, Ting Du, Maiken Nedergaard
Article
Biology
Aditya Raghunandan, Antonio Ladron-de-Guevara, Jeffrey Tithof, Humberto Mestre, Ting Du, Maiken Nedergaard, John H. Thomas, Douglas H. Kelley
Summary: New in vivo experiments have addressed the hypothesis that bulk flow of cerebrospinal fluid might be driven by the injection method, showing consistent particle-tracking flows with earlier experiments despite using a dual-syringe system that does not increase CSF volume or intracranial pressure.
Article
Clinical Neurology
Ting Du, Humberto Mestre, Benjamin T. Kress, Guojun Liu, Amanda M. Sweeney, Andrew J. Samson, Martin Kaag Rasmussen, Kristian Nygaard Mortensen, Peter A. R. Bork, Weiguo Peng, Genaro E. Olveda, Logan Bashford, Edna R. Toro, Jeffrey Tithof, Douglas H. Kelley, John H. Thomas, Poul G. Hjorth, Erik A. Martens, Rupal Mehta, Hajime Hirase, Yuki Mori, Maiken Nedergaard
Summary: Cerebral edema develops after anoxic brain injury and is associated with the availability of cerebrospinal fluid (CSF). An increase in CSF volume worsens the severity of edema. Edema primarily occurs in brain regions bordering CSF compartments. Anoxic brain tissue possesses a high intrinsic osmotic potential, which may contribute to the beneficial effect of therapeutic hypothermia.
Review
Neurosciences
Guojun Liu, Antonio Ladron-de-Guevara, Yara Izhiman, Maiken Nedergaard, Ting Du
Summary: Cerebrospinal fluid (CSF) is a critical component of the central nervous system, providing protection and transport functions. However, there is limited knowledge about its production rate and the impact of diseases on CSF dynamics. This review aims to highlight the importance of quantifying CSF production and inspire the development of more accurate and less invasive measurement techniques.
FLUIDS AND BARRIERS OF THE CNS
(2022)
Article
Multidisciplinary Sciences
Kimberly A. S. Boster, Shengze Cai, Antonio Ladron-de-Guevara, Jiatong Sun, Xiaoning Zheng, Ting Du, John H. Thomas, Maiken Nedergaard, George Em Karniadakis, Douglas H. Kelley
Summary: This study demonstrates the use of artificial intelligence velocimetry (AIV) to quantify the flow of cerebrospinal fluid (CSF) in pial perivascular spaces (PVSs) by integrating sparse velocity measurements with physics-informed neural networks. AIV infers three-dimensional high-resolution velocity, pressure, and shear stress, and the results are validated and analyzed for sensitivity. The findings contribute to improving fluid dynamic models and potentially providing insights into how CSF flow changes in aging, Alzheimer's disease, and small vessel disease.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Multidisciplinary Sciences
Fatima Min Rivas, Jia Liu, Benjamin C. Martell, Ting Du, Humberto Mestre, Maiken Nedergaard, Jeffrey Tithof, John H. Thomas, Douglas H. Kelley
JOURNAL OF THE ROYAL SOCIETY INTERFACE
(2020)
Meeting Abstract
Clinical Neurology
Humberto Mestre, Ting Du, Andrew Samson, Benjamin Kress, Amanda Sweeney, Martin Kaag Rasmussen, Kristian Nygaard Mortensen, Yuki Mori, Maiken Nedergaard
Article
Medicine, Research & Experimental
Benjamin A. Plog, Humberto Mestre, Genaro E. Olveda, Amanda M. Sweeney, H. Mark Kenney, Alexander Cove, Kosha Y. Dholakia, Jeffrey Tithof, Thomas D. Nevins, Iben Lundgaard, Ting Du, Douglas H. Kelley, Maiken Nedergaard