Review
Biochemistry & Molecular Biology
Hidekazu Sekine, Teruo Okano
Summary: One of the key challenges in regenerative medicine is to introduce vascular networks into bioengineered tissues. Co-culturing transplant tissue with vascular cells before transplantation has been explored as a way to promote capillary angiogenesis and construct a continuous circulatory structure.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biotechnology & Applied Microbiology
WonJin Kim, GeunHyung Kim
Summary: A new cell-printing process is introduced in this study, which allows the fabrication of cell-spheroids and cell-loaded constructs together without the need to prepare cell-spheroids in advance. The diameter and location of spheroids can be manipulated by controlling printing parameters. Hybrid cell constructs fabricated using this method exhibit strong angiogenic and osteogenic activities.
BIOENGINEERING & TRANSLATIONAL MEDICINE
(2023)
Review
Engineering, Biomedical
Leanne de Silva, Paulina N. Bernal, A. J. W. Rosenberg, Jos Malda, Riccardo Levato, Debby Gawlitta
Summary: The development of tissue engineering strategies for treatment of large bone defects is crucial, and creating a vascular network is vital for tissue engineered bone constructs.
ACTA BIOMATERIALIA
(2023)
Article
Engineering, Environmental
WonJin Kim, Hyeongjin Lee, Eun Ji Roh, Seong Bae An, In-Bo Han, Geun Hyung Kim
Summary: The bioprinted cell-laden construct consisting of collagen, beta-tricalcium phosphate, human adipose stem cells (hASCs) and human umbilical vein endothelial cells (HUVECs) was successfully used for bone tissue regeneration in spinal fusion. The construct exhibited strong angiogenic and osteogenic activities due to the synergistic crosstalk of the two cell types, leading to significantly higher new bone formation and angiogenesis in a mouse model of posterolateral lumbar spinal fusion. These findings suggest that biomimetic cell-laden constructs could potentially encourage effective spinal fusion by promoting bone tissue regeneration.
CHEMICAL ENGINEERING JOURNAL
(2022)
Article
Biochemistry & Molecular Biology
Isabel Verit, Laura Gemini, Julie Preterre, Pierre Pfirmann, Hugo Bakis, Jean-Christophe Fricain, Rainer Kling, Claire Rigothier
Summary: Research investigates the formation of intra-volume channels within three-dimensional collagen microfibers using laser-induced cavitation to address the lack of efficient vascularization systems for organ transplantation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Engineering, Biomedical
Yongcong Fang, Mengke Ji, Yi Yang, Yihan Guo, Ruobin Sun, Ting Zhang, Wei Sun, Zhuo Xiong
Summary: Three-dimensional bioprinting has been hindered by a lack of suitable bioinks for printing functional tissues. We developed a granular cell aggregate-based biphasic (GCAB) bioink that exhibited desired properties for extrusion bioprinting and modeling the mechanical characteristics of soft tissues. By preorganizing GCAB bioink and printing it with an endothelial cell-laden gelatin bioink, we successfully created functional hepatic tissues with high cell density and a perfusable vascular network. This GCAB bioink design has potential for creating functional tissues for therapeutic applications.
Article
Chemistry, Multidisciplinary
Ju An Park, Yejin Youm, Hwa-Rim Lee, Yongwoo Lee, Sarah L. Barron, Taejeong Kwak, Gyu Tae Park, Young-Cheol Song, Roisin M. Owens, Jae Ho Kim, Sungjune Jung
Summary: A cell sheet transfer method from a culture surface to a target tissue is introduced in this study. UV-treated parylene, a commercially available flexible material, is used as the culture surface to provide adequate cell adhesiveness for both cell attachment during culture and efficient transfer. Various cell types are successfully transferred using this versatile cell-transfer process. The mechanism of interfacial cell migration between surfaces with different adhesion preferences is observed, and the re-establishment and reversal of cell polarity after transfer are identified. The unique capabilities of 3D multilayer stacking, freeform design, and curved surface application are demonstrated. The therapeutic potential of the cell-sheet delivery system is demonstrated through experiments on cutaneous wound healing and skin-tissue regeneration in mice models.
ADVANCED MATERIALS
(2023)
Article
Engineering, Biomedical
Qilong Zhao, Yu Zhou, Min Wang
Summary: This study demonstrates a new method for directly placing living endothelial cells within bioactive nanofibrous scaffolds in 3D, using concurrent emulsion electrospinning and coaxial cell electrospraying. The technique allows for deep cell distribution and preservation of cell viability, leading to promising bioactive nanofibrous scaffolds with 3D cell incorporation. By combining structural and biochemical cues, the 3D cell-incorporated scaffolds offer an innovative approach to tissue engineering for creating vascularized structures.
ACTA BIOMATERIALIA
(2021)
Article
Biophysics
Zhiwei Jiang, Jin He, Xueting Wang, Danji Zhu, Na Li, Lingfei Ren, Guoli Yang
Summary: Nanomaterial-based cell sheet technology has significant potential in regenerative medicine and tissue engineering. Different types of nanomaterials have been used to harvest cell sheets, categorized by mechanisms such as light-induced, thermo-responsive, magnetic-controlled, and ROS-induced. Studies have demonstrated successful outcomes in the regeneration of bone, skeletal muscle, cardiac tissue, tendon, angiogenesis, and osseointegration. However, challenges remain in terms of comprehensive preparation, safety, and cell quality.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2022)
Review
Engineering, Biomedical
Zhiwei Jiang, Danji Zhu, Ke Yu, Yue Xi, Xiaozhao Wang, Guoli Yang
Summary: Cell sheet technology is a convenient and efficient approach for tissue regeneration and cell therapy, but faces limitations that need further research to overcome. Changes in culturing surfaces under light illumination lead to cell sheet detachment, highlighting the need for developing new materials for rapid and effective cultivation and release of cell sheets in future studies.
ACTA BIOMATERIALIA
(2021)
Article
Engineering, Biomedical
Laura Elomaa, Marcus Lindner, Ruth Leben, Raluca Niesner, Marie Weinhart
Summary: This study combines cell sheet engineering, 3D printing, and cellular self-organization to create a vascular-like 3D network. The results demonstrate that a cell sheet-covered vessel mimic and self-assembling vascular endothelial cells can spontaneously form a dense vascular network in a collagen gel under specific culture conditions.
Review
Engineering, Multidisciplinary
Diana S. Lim, John D. Jackson, Anthony Atala, James J. Yoo
Summary: There is a pressing need for new treatments for renal failure, but current therapies are limited by the scarcity of viable organs and infrastructure. Kidney tissue engineering holds promise in addressing the shortcomings, but faces challenges, particularly in establishing successful vascularization of engineered tissue constructs.
Review
Polymer Science
Ilya L. Tsiklin, Aleksey V. Shabunin, Alexandr V. Kolsanov, Larisa T. Volova
Summary: Reconstruction of critical-sized bone defects remains a challenge for surgeons worldwide. Bone tissue engineering offers a novel approach using seed cells, growth factors, and bioactive scaffolds. Insufficient vascularization is currently a critical limitation for viability. The in vivo bioreactor principle shows promise for predictable bone tissue regeneration.
Review
Biochemistry & Molecular Biology
Chikahiro Imashiro, Tatsuya Shimizu
Summary: Tissue engineering has been attracting significant attention since the 1980s, with cell sheet technology being a promising strategy to produce cell-dense tissues. Various techniques for fabricating and improving cell sheets have been studied, with the goal of mimicking physiological conditions and promoting vasculogenesis. This review highlights fundamental technologies and recent advances in cell-sheet-based tissue engineering, aiming to further develop tissue engineering applications.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Multidisciplinary
Malin Becker, Melvin Gurian, Maik Schot, Jeroen Leijten
Summary: Embedded 3D bioprinting using low-viscosity ink 3D (LoV3D) bioprinting based on aqueous two-phase stabilization allows for the fabrication of living constructs at high speeds with high viability. The liquid/liquid interfaces of LoV3D bioprinting offer unique advantages for fusing structures, creating vasculature, and modifying surfaces. The low interfacial tension of LoV3D bioprinting allows for nozzle-independent control over filament diameter, enabling the printing of a wide range of diameters down to the width of a single cell.
Article
Engineering, Biomedical
Jun Akimoto, Masamichi Nakayama, Soichi Takagi, Teruo Okano
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
(2019)
Article
Cell & Tissue Engineering
Katsuhisa Matsuura, Masanori Wada, Katsuhisa Sakaguchi, Yuki Matsuhashi, Tatsuya Shimizu
REGENERATIVE THERAPY
(2019)
Article
Cell & Tissue Engineering
Botao Gao, Katsuhisa Sakaguchi, Katsuhisa Matsuura, Tetsuya Ogawa, Yuki Kagawa, Hirotsugu Kubo, Tatsuya Shimizu
TISSUE ENGINEERING PART A
(2020)
Article
Engineering, Biomedical
Hiroaki Takehara, Katsuhisa Sakaguchi, Hidekazu Sekine, Teruo Okano, Tatsuya Shimizu
BIOMEDICAL MICRODEVICES
(2020)
Editorial Material
Cell & Tissue Engineering
Kyungsook Kim, David W. Grainger, Teruo Okano
REGENERATIVE THERAPY
(2019)
Article
Cell & Tissue Engineering
Shinpei Tsuruyama, Katsuhisa Matsuura, Katsuhisa Sakaguchi, Tatsuya Shimizu
REGENERATIVE THERAPY
(2019)
Article
Cell & Tissue Engineering
Katsuhisa Sakaguchi, Hiroaki Takahashi, Yusuke Tobe, Daisuke Sasaki, Katsuhisa Matsuura, Kiyotaka Iwasaki, Tatsuya Shimizu, Mitsuo Umezu
TISSUE ENGINEERING PART C-METHODS
(2020)
Article
Cell & Tissue Engineering
Botao Gao, Katsuhisa Sakaguchi, Tetsuya Ogawa, Yuki Kagawa, Hirotsugu Kubo, Tatsuya Shimizu
Summary: The study demonstrated that induced-BHs (iBHs) affect urea synthesis and cytochrome P450 enzyme activities, as well as lead to loss of bile canaliculi in co-culture cell sheets. Additionally, higher secretion of TGF-beta 1 was observed in iBHs. Treatment with a TGF-beta inhibitor and a semi-synthetic bile acid analogue improved the histological appearance of established iBHs.
TISSUE ENGINEERING AND REGENERATIVE MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Chikahiro Imashiro, Kai Yamasaki, Ryu-ichiro Tanaka, Yusuke Tobe, Katsuhisa Sakaguchi, Tatsuya Shimizu
Summary: This study introduces a novel system that enhances transportation in cell tissues through diffusion and convection, improving tissue viability and promoting glucose consumption, lactate production, and oxygen transport. The proposed system potentially sets a new standard for 3D cell cultures.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Engineering, Biomedical
Katsuhisa Sakaguchi, Yusuke Tobe, Jiayue Yang, Ryu-ichiro Tanaka, Kumiko Yamanaka, Jiro Ono, Tatsuya Shimizu
Summary: The article introduces a novel tissue engineering method using a net metal mould without the use of a scaffold, which allows for the formation of 3D tissues by culturing human dermal fibroblasts. The study successfully constructs scaffold-free 3D tissues with controllable strength, which may have a significant impact on next-generation tissue engineering products.
Article
Biotechnology & Applied Microbiology
Chikahiro Imashiro, Takashi Morikura, Motoaki Hayama, Atsushi Ezura, Jun Komotori, Shogo Miyata, Katsuhisa Sakaguchi, Tatsuya Shimizu
Summary: This article introduces the method of using 3D printing of metal to manufacture culture devices and its potential and application in bioengineering research. By creating mesh structures on the culture surfaces, the culture environment of tissues can be improved, leading to enhanced nutrient supply. The technology of manufacturing culture devices using metal 3D printing demonstrates good biocompatibility and reusability.
BIOTECHNOLOGY AND BIOPROCESS ENGINEERING
(2023)
Article
Food Science & Technology
Ryu-ichiro Tanaka, Katsuhisa Sakaguchi, Azumi Yoshida, Hironobu Takahashi, Yuji Haraguchi, Tatsuya Shimizu
Summary: Cell sheet-based meat, produced using cell sheet technology without a scaffold, demonstrates similar texture and nutrients to natural meat. This method offers the potential for environmentally friendly food production and can be scaled up as needed.
NPJ SCIENCE OF FOOD
(2022)
Article
Multidisciplinary Sciences
Nobuhiro Hasui, Katsuhisa Sakaguchi, Tetsuya Ogawa, Yoshihiro Sakamoto, Tatsuya Shimizu
Summary: In this study, a new in vitro model of ballooned hepatocytes in NASH was developed using engineered cell sheets. The model successfully replicated the histological features of ballooned hepatocytes in human NASH and revealed the underlying mechanism of fibrosis contributing to the development of ballooned hepatocytes.
SCIENTIFIC REPORTS
(2022)
Article
Engineering, Biomedical
Katsuhisa Sakaguchi, Kei Akimoto, Masanori Takaira, Ryu-ichiro Tanaka, Tatsuya Shimizu, Shinjiro Umezu
Summary: The development of microelectromechanical systems has led to the rapid advancement of PDMS microfluidic devices in drug screening models. In this study, a microfluidic device consisting only of cells was developed, which may contribute to the development of organ-on-a-chip systems.
CYBORG AND BIONIC SYSTEMS
(2022)
Article
Robotics
Ryu-ichiro Tanaka, Katsuhisa Sakaguchi, Shinjiro Umezu
ARTIFICIAL LIFE AND ROBOTICS
(2020)
Review
Chemistry, Multidisciplinary
Zhiyuan Shi, Yong Hu, Xin Li
Summary: Controlled drug delivery systems that can respond to mechanical force offer a unique solution for on-demand drug activation and release. Among various mechanical stimuli, ultrasound (US) has advantages in achieving spatiotemporally controlled drug release. Traditional US-triggered drug release relies on heat-induced phase transitions or chemical transformations, while the cutting-edge approach of Sonopharmacology leverages polymer mechanochemistry. The remaining challenges and potential future directions in this field are also discussed.
JOURNAL OF CONTROLLED RELEASE
(2024)
Article
Chemistry, Multidisciplinary
Lijie Zheng, Yuanzheng Chen, Xun Gu, Yingying Li, Hanqing Zhao, Wenjun Shao, Tao Ma, Chuanbin Wu, Qingqing Wang
Summary: In this study, a novel dosage form consisting of dissolving microneedles and an adhesive transdermal patch was developed for the treatment of rheumatoid arthritis. In vitro and in vivo experiments demonstrated that the combination of drugs delivered by this dosage form effectively reduced joint inflammation and damage.
JOURNAL OF CONTROLLED RELEASE
(2024)
Article
Chemistry, Multidisciplinary
Kyungjik Yang, Hwa Seung Han, Seung Hwan An, Kyung Hoon Park, Keonwook Nam, Shinha Hwang, Yuyeon Lee, Sung Yeon Cho, Taehyung Kim, Deokyeong Choe, Sang Won Kim, Wonkyu Yu, Hyunah Lee, Jiyong Park, Sangguan You, Dong- Gyu Jo, Ki Young Choi, Young Hoon Roh, Jae Hyung Park
Summary: This study developed CP-loaded CS microcapsules to enhance the oral bioavailability of CP through controlled gastrointestinal delivery. The optimized microcapsules exhibited desirable physicochemical properties, showed anti-photoaging effects via antioxidant activity, and achieved controlled release in the gastrointestinal tract. This research provides a simple and economical approach for enhancing the oral bioavailability of CP for customized bioactive compound administration.
JOURNAL OF CONTROLLED RELEASE
(2024)
Article
Chemistry, Multidisciplinary
Huiyang Li, Shuo Liu, Wenjin Dai, Bingmei Yao, Yong Zhou, Sujia Si, Hairong Yu, Riguang Zhao, Fang Jin, Liqun Jiang
Summary: Changes in bodily fluid pressures are crucial in diseases like high-altitude pulmonary edema (HAPE). Researchers have developed hydrostatic pressure-sensitive multivesicular liposomes (PSMVLs) that can release drugs in response to pressure changes, with potential applications in HAPE treatment. Animal experiments showed that this system provides better protection for lung tissues and respiratory function, reducing the occurrence of pulmonary edema.
JOURNAL OF CONTROLLED RELEASE
(2024)
Article
Chemistry, Multidisciplinary
Qian Hu, Hongbing Lan, Yinmei Tian, Xiaonan Li, Mengmeng Wang, Jiao Zhang, Yulin Yu, Wei Chen, Li Kong, Yuanyuan Guo, Zhiping Zhang
Summary: Coacervate droplets formed through liquid-liquid phase separation have potential as delivery vesicles for therapeutics. However, their lack of physiological stability and membranes are challenges. In this study, polylysine-polynucleotide complex coacervate droplets with favorable stability were formulated to concentrate molecules and nanoparticles. Phospholipid membranes were further coated on the droplets to create coacervate-based artificial protocells (ArtPC) with membrane-like structures. These biofunctional ArtPC effectively reduced blood uric acid levels and prevented renal injuries.
JOURNAL OF CONTROLLED RELEASE
(2024)
Article
Chemistry, Multidisciplinary
Xiaowei Wang, Hongwei Lu, Fang Luo, Dan Wang, Apeng Wang, Xuelei Wang, Wenkai Feng, Xiaobo Wang, Jiayi Su, Mingliang Liu, Guimin Xia
Summary: Four novel lipid-like GEM diesters were synthesized and encapsulated into liposomes to improve the antitumor efficacy of Gemcitabine. The liposomes loaded with dimyristoyl GEM (LipodmGEM) showed enhanced cellular uptake, improved inhibition of cell migration, and a greatly extended half-life compared to free Gemcitabine. LipodmGEM successfully enriched the drug in the tumor and exhibited excellent anticancer efficacy in vivo with negligible systemic toxicity.
JOURNAL OF CONTROLLED RELEASE
(2024)
Article
Chemistry, Multidisciplinary
Pengyu Li, Jieyi Pan, Yating Dong, Yingying Sun, Yalong Wang, Kang Liao, Yili Chen, Xin Deng, Shihui Yu, Haiyan Hu
Summary: Chronic pulmonary infection caused by Pseudomonas aeruginosa is a serious public health problem with high mortality rates. In this study, infection-microenvironment responsive nanoparticles were developed to eradicate biofilms and inhibit virulence. These nanoparticles showed promising results in treating chronic pulmonary infections.
JOURNAL OF CONTROLLED RELEASE
(2024)
Article
Chemistry, Multidisciplinary
Yajing Sun, Ze Lu, John A. Taylor, Jessie L. S. Au
Summary: A recent development in cancer chemotherapy is the use of cytotoxics to induce tumor-specific immune response through immunogenic cell death (ICD). This study describes a method that utilizes immunostaining and machine-learning to identify cells with ecto-CRT in intact 3-dimensional tissues. The method was successfully applied to study drug-induced ICD in human bladder cancer.
JOURNAL OF CONTROLLED RELEASE
(2024)
Review
Chemistry, Multidisciplinary
Rafat Ali, Shantanu Sen, Rohil Hameed, Aamir Nazir, Sandeep Verma
Summary: This review provides a focused overview of emerging strategies for delivering gasotransmitters in a controlled and sustained manner to reestablish neurophysiological homeostasis.
JOURNAL OF CONTROLLED RELEASE
(2024)
Article
Chemistry, Multidisciplinary
Jing Chen, Xinyi Zhang, Jinshen Zhang, Zhaoxia Wang, Guilan Zhu, Ming Geng, Jinmiao Zhu, Yajun Chen, Wei Wang, Youcui Xu
Summary: In this study, a multifunctional responsive hydrogel system was developed for synergistic reoxygenation and chemo/photothermal therapy. The hydrogel system showed both therapeutic effects against metastatic breast cancer and wound infection, making it a promising strategy for treating and preventing tumor recurrence and associated wound infection.
JOURNAL OF CONTROLLED RELEASE
(2024)
Article
Chemistry, Multidisciplinary
Baoshan Huang, Na Zhang, Xinying Qiu, Rui Zeng, Shuimiao Wang, Mengxia Hua, Qing Li, Kaihui Nan, Sen Lin
Summary: This study revealed that robust ROS can oxidize mitochondrial DNA (ox-mtDNA) and cause its release into the cytosol, resulting in the activation of NLRP3 inflammasome. By using the mitochondria-targeted antioxidant SkQ1 and a novel mitochondria-targeted nanoparticle (SkQ1 NP), it was found that mitochondrial ROS scavenging could in situ inhibit DED-induced mtDNA oxidation and suppress NLRP3-mediated inflammation.
JOURNAL OF CONTROLLED RELEASE
(2024)
Article
Chemistry, Multidisciplinary
Wenqi Liu, Cheng Hu, Linyu Long, Shuyi He, Wen Zhang, Zhicun Wang, Li Yang, Yunbing Wang
Summary: Myocardial infarction is the leading cause of cardiovascular mortality, and current treatment methods have limitations. This study developed a smart carrier that can release different therapeutic substances for different pathological processes, effectively improving cardiac function, promoting cardiac repair, and preventing ventricular remodeling.
JOURNAL OF CONTROLLED RELEASE
(2024)