Article
Chemistry, Multidisciplinary
David S. Spencer, Aaliyah B. Shodeinde, David W. Beckman, Bryan C. Luu, Hannah R. Hodges, Nicholas A. Peppas
Summary: By evaluating a series of nanoparticle formulations, this study identified candidates with low cytotoxicity, pH-dependent membrane disruption potential, highest siRNA loading, and transfection efficacy for in vitro applications.
JOURNAL OF CONTROLLED RELEASE
(2021)
Article
Engineering, Environmental
Jian Shu, Xudong Li, Juanjuan Dang, Yong Liu, Shanzhou Duan, Rongying Zhu, Lichen Yin, Yongbing Chen
Summary: This study presents a promising strategy to reverse drug resistance in cancer by intervening in cancer bioenergetics using RNA interference. The development of a densely packed, guanidine-rich spherical helical polypeptide for encapsulating chemotherapeutic drugs and siRNA shows potential for enhancing drug delivery and inhibiting drug efflux. The down-regulation of PKM2 through RNA interference effectively suppresses glycolysis metabolism, depletes energy supply for drug transporters, and induces apoptosis, thereby improving the efficacy of chemotherapy against drug-resistant tumors.
CHEMICAL ENGINEERING JOURNAL
(2021)
Article
Chemistry, Multidisciplinary
Xiang Yu, Hai Xiao, Muqier Muqier, Shuqin Han, Huricha Baigude
Summary: In this study, we investigated the influence of type and number of amino groups on the neural cell targeted nucleic acid delivery by designing lipid with different amine-containing branched head groups. The results suggest that the lipid with 3 amino groups showed the highest nucleic acid transfection efficiency to neural cells.
Article
Medicine, General & Internal
Adam A. Walters, Baljevan Dhadwar, Khuloud T. Al-Jamal
Summary: Immune checkpoint targeting immunotherapy aims to manipulate interactions to engage the immune system in cancer treatment. Clinical use of monoclonal antibodies to block immunosuppressive interactions has been effective. Next generation strategies involve altering expression levels of immune checkpoint molecules with nucleic acids, including formulation within nanoparticulate systems.
Review
Medicine, Research & Experimental
Santoshi Naik, Ajjappla Basavaraj Shreya, Ruchira Raychaudhuri, Abhijeet Pandey, Shaila A. Lewis, Manali Hazarika, Sulatha Bhandary, Bola Sadashiva Satish Rao, Srinivas Mutalik
Summary: This article discusses the current research on using siRNA-based gene silencing strategies to treat glaucoma, including discussions on ocular structures, administration routes, viral and non-viral vectors, as well as basic strategies and challenges in siRNA therapy. Different therapeutic targets for glaucoma, current siRNA-based drugs used in treatment, and strategies for siRNA-based treatment after glaucoma surgery are also briefly mentioned.
Review
Pharmacology & Pharmacy
Dima Hattab, Amirah Mohd Gazzali, Athirah Bakhtiar
Summary: RNA interference (RNAi) offers a genetic approach for cancer treatment by inhibiting specific messenger RNA signaling uncontrollable cell growth and proliferation. Challenges such as low tumor localization, stability and rapid clearance from circulation hinder the success of gene therapy applications.
Article
Biochemistry & Molecular Biology
Anna S. Pavlova, Kristina I. Yakovleva, Anna V. Epanchitseva, Maxim S. Kupryushkin, Inna A. Pyshnaya, Dmitrii V. Pyshnyi, Elena I. Ryabchikova, Ilya S. Dovydenko
Summary: The study showed that siRNA modified with the phosphoryl guanidine (PG) group exhibited increased resistance to RNase A but decreased thermodynamic stability. However, PG-modified siRNA retained its activity when the modifications were introduced into the passenger strand.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Pharmacology & Pharmacy
Min Ju Kim, Hyeyoun Chang, Gihoon Nam, Youngji Ko, Sun Hwa Kim, Thomas M. Roberts, Ju Hee Ryu
Summary: Pancreatic cancer, predicted to be the second leading cause of cancer-associated death by 2025, remains a lethal form of cancer with low patient survival rates. RNA interference (RNAi) has emerged as a promising approach for pancreatic cancer treatment, with the use of nanocarriers to overcome delivery challenges. Studies have shown that targeting either the tumor or tumor microenvironment using siRNA and miRNA can induce a strong anticancer effect in pancreatic cancer models.
Article
Materials Science, Multidisciplinary
Xiaolong Gao, Junjun Tang, Minghua Li, Chao Lin, Peijun Wang
Summary: Gadolinium-based small-molecule contrast agents have short retention time and cytotoxicity, but poly(urethane-amine) polymer can enhance their effects and be used as a gene carrier for siRNAs. A nanocomposite system with active targeting for tumor cells was developed using this polymer, incorporating Gd-diethylenetriamine pentaacetate contrast agents and siRNA-VEGF for both imaging improvement and tumor growth inhibition. The system demonstrated good biocompatibility and integration of tumor diagnosis and treatment.
ACS APPLIED POLYMER MATERIALS
(2022)
Article
Chemistry, Multidisciplinary
Jong Won Lee, Jiwon Choi, Yeonho Choi, Kwangmeyung Kim, Yoosoo Yang, Sun Hwa Kim, Hong Yeol Yoon, Ick Chan Kwon
Summary: RNA interference (RNAi) is a cellular mechanism that regulates gene expression, with small interfering RNA molecules (siRNA) mediating mRNA degradation. Despite the promising therapeutic potential of RNAi technology for diseases like cancer, challenges such as biological instability and limited delivery efficiency remain. Recent advancements in siRNA delivery strategies using cationic lipids and polymers have shown improved pharmacokinetics and delivery efficiency.
JOURNAL OF CONTROLLED RELEASE
(2022)
Article
Pharmacology & Pharmacy
Muhammad Imran Sajid, Dindyal Mandal, Naglaa Salem El-Sayed, Sandeep Lohan, Jonathan Moreno, Rakesh Kumar Tiwari
Summary: Recent approvals of siRNA-based products have led to exploration of siRNA as a potential treatment option for cancer and other diseases. This study introduces a series of oleyl-conjugated histidine-arginine peptides as a promising nonviral siRNA delivery tool.
Review
Microbiology
Javad Khanali, Mohammadreza Azangou-Khyavy, Yasaman Asaadi, Monire Jamalkhah, Jafar Kiani
Summary: Despite the lack of approved treatments for COVID-19, biotechnological approaches such as aptamers and siRNAs show promise. These nucleic acid-based therapies have the potential to hinder viral entry, replication, and reduce viral loads, suggesting their application in combating SARS-CoV-2.
FRONTIERS IN MICROBIOLOGY
(2021)
Review
Chemistry, Multidisciplinary
Jinho Yoon, Minkyu Shin, Ji-Young Lee, Sang-Nam Lee, Jin-Ha Choi, Jeong-Woo Choi
Summary: RNA interference (RNAi)-based therapeutics have not been used for cancer treatment due to their instability and microRNA (miRNA) delivery challenges. Plasmonic nanoparticle-based RNAi nanotherapeutics have been developed with specific properties for accurate cancer diagnosis and strong therapeutic effects. This review presents recent strategies and advances in plasmonic nanoparticle-based miRNA delivery for cancer detection and treatment, and discusses the challenges and potential opportunities in the field of RNAi-based theragnosis.
JOURNAL OF CONTROLLED RELEASE
(2022)
Review
Medicine, Research & Experimental
Peipei Wang, Yue Zhou, Arthur M. Richards
Summary: The comparison between siRNA and miRNA suggests that miRNA mimics have unique advantages due to their endogenous nature and the ability to regulate gene silencing effects, which will facilitate the development of miRNA mimic drugs.
Article
Biochemistry & Molecular Biology
Zhiqing Zhang, Dongyan Xu, Jiawei Wang, Ruyan Zhang, Huan Du, Ting Zhou, Xiufeng Wang, Fang Wang
Summary: This study designed a DNA nano-assembly based on rolling circle amplification that can co-deliver doxorubicin (Dox) and siRNA simultaneously, achieving a comprehensive effect in tumor treatment.
Article
Biochemistry & Molecular Biology
Tianjian Yang, Kyle Benson, Hailin Fu, Tianrui Xue, Ziyuan Song, Hanyi Duan, Hongwei Xia, Ankarao Kalluri, Jie He, Jianjun Cheng, Challa Kumar, Yao Lin
Summary: This study demonstrates the accelerated assembly of synthetic poly(L-glutamic acid) into amyloid-like fibrils catalyzed by cationic silica nanoparticle clusters as artificial nucleation factors. The charge-charge interactions and surface functionalities effectively facilitate the conformational change of macromolecular monomers and control the rates of nucleation for fibril growth. This approach inspires the development of novel materials through biomimetic supramolecular polymerizations.
Article
Polymer Science
Mohammad Hakim Khalili, Ashfaq Afsar, Rujing Zhang, Sandra Wilson, Eleftheria Dossi, Saurav Goel, Susan A. Impey, Adrianus Indrat Aria
Summary: In this study, the thermal response of multi-layered PEGDA hydrogels was investigated under various temperature-controlled environments. The results showed that the volume of the hydrogels changed at different temperatures, and they also exhibited anisotropic characteristics.
POLYMER DEGRADATION AND STABILITY
(2022)
Article
Materials Science, Multidisciplinary
Christina Schmidleithner, Rujing Zhang, Nayere Taebnia, Esben K. U. Larsen, Kristoffer Almdal, Niels B. Larsen
Summary: A high-resolution dual-material 3D printing concept is developed, allowing for the production of distinct materials locally based on orthogonal chemical reactions depending on the illumination wavelength. The resulting devices have micrometer-scale precision in tissue modeling.
ADVANCED MATERIALS TECHNOLOGIES
(2022)
Article
Oncology
Justin Watts, Tara L. Lin, Alice Mims, Prapti Patel, Cynthia Lee, Anoush Shahidzadeh, Paul Shami, Elizabeth Cull, Christopher R. Cogle, Eunice Wang, Fatih M. Uckun
Summary: APVO436 is a recombinant bispecific antibody that could be used to treat hematologic malignancies, particularly relapsed or refractory AML/MDS patients. Preliminary clinical trial results showed promising tolerability and activity of APVO436 in these patients, with activation of T cells.
FRONTIERS IN ONCOLOGY
(2022)
Article
Polymer Science
Hailin Fu, Ryan Baumgartner, Ziyuan Song, Chongyi Chen, Jianjun Cheng, Yao Lin
Summary: This article introduces a simple kinetic model to describe the cooperativity in covalent polymerizations. By treating the growing polymer chain as both a cooperative system and a primitive enzyme, and incorporating a Michaelis-Menten-like equation, the model accurately describes the binding of monomers to the polymer chain. The application of this model can help polymer chemists discover similar cooperative mechanisms in nonpolypeptide systems and utilize them to create covalent analogues of natural cooperative systems.
Article
Oncology
Deniz C. Guven, Taha K. Sahin, Serkan Akin, Fatih M. Uckun
Summary: Patients with hematologic malignancies, especially those with CLL and those receiving specific treatments, have significantly lower seroconversion rates after SARS-CoV-2 vaccination. Effective strategies are urgently needed to improve vaccine efficacy in these vulnerable populations.
Article
Polymer Science
Fangping Yang, Zhiwei Liu, Wenting Si, Ziyuan Song, Lichen Yin, Haoyu Tang
Summary: Polysaccharide-polypeptide conjugates have shown great potential for biomedical applications by mimicking the structure and bioactivity of peptidoglycans or proteoglycans. However, the efficient and precise preparation of these conjugates remains challenging. In this study, we present a facile and efficient strategy to prepare conjugates with well-controlled polypeptide chain length from unmodified polysaccharides. The effect of polypeptide chain length on the surface antibacterial properties was also investigated.
Article
Microbiology
Fatih M. Uckun, Muhammad Saeed, Mustafa Awili, Ibrahim H. Ozercan, Sanjive Qazi, Cynthia Lee, Adeel Shibli, Alan W. Skolnick, Alonso Prusmack, Joseph Varon, Cesar I. P. Barrera, Cemal Orhan, Michael Volk, Kazim Sahin
Summary: This study compared the effects of Rejuveinix, dexamethasone, and their combination on fatal sepsis in an animal model and COVID-19 patients. The results showed that RJX exhibited potent anti-inflammatory activity, reducing cytokine responses and organ damage in mice, and improving survival outcome. The clinical study also demonstrated that RJX plus DEX was well tolerated by COVID-19 patients.
FUTURE MICROBIOLOGY
(2022)
Letter
Oncology
Deniz C. Guven, Taha K. Sahin, Serkan Akin, Fatih M. Uckun
Article
Chemistry, Multidisciplinary
Chenglong Ge, Junliang Zhu, Huan Ye, Yuansong Wei, Yuheng Lei, Renxiang Zhou, Ziyuan Song, Lichen Yin
Summary: In this study, a series of protein-mimetic nano-switches were constructed to control their function based on the flexibility/rigidity of the polymeric chains. When in a dormant state, the polypeptide adopts a flexible, random-coiled conformation, leading to the turn-off of the optically functional molecule. Upon treatment with alkaline phosphatase, the nano-switches are activated due to the coil-to-helix and flexible-to-rigid transition of the polypeptide linker, enabling selective fluorescence imaging or photodynamic therapy in response to ALP overproduced by tumor cells.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Article
Oncology
Fatih M. Uckun, Sanjive Qazi, Vuong Trieu
Summary: This study evaluated the clinical significance of amplified expression levels of transforming growth factor beta 2 (TGFB2) in tumor tissue specimens from pediatric DIPG patients. The results showed that high level TGFB2 expression is associated with poor treatment outcome in DIPG. These findings support further evaluation of new strategies targeting TGFB2 in pediatric DIPG.
Article
Oncology
Sanjive Qazi, Fatih M. Uckun
Summary: This study evaluated the clinical significance of CD22 exon 12 deletion and found that it is associated with poor treatment outcomes in B-ALL. The results suggest that further evaluation of targeting this abnormality in B-ALL is warranted.
Article
Health Care Sciences & Services
Sanjive Qazi, Fatih M. Uckun
Summary: We investigated the expression of ErbB family protein tyrosine kinases, including ERBB1, at the transcript level in primary malignant lymphoma cells from 498 adult patients with diffuse large B-cell lymphoma (DLBCL). The expression of ERBB1 in DLBCL cells was significantly higher compared to normal B-lineage lymphoid cells. Increased ERBB1 mRNA expression in DLBCL cells was correlated with amplified expression of transcription factors that recognize ERBB1 gene promoter sites. Importantly, amplified ERBB1 expression in DLBCL and its subtypes was associated with significantly worse overall survival (OS). These findings support further evaluation of the prognostic significance of high-level ERBB1 mRNA expression and the clinical potential of personalized medicines targeting ERBB1 in high-risk DLBCL.
JOURNAL OF PERSONALIZED MEDICINE
(2023)
Article
Chemistry, Multidisciplinary
Wanying Wang, Hailin Fu, Yao Lin, Jianjun Cheng, Ziyuan Song
Summary: In this paper, we discussed the mechanism and applications of cooperative covalent polymerization (CCP), which can accelerate the polymerization reaction and efficiently synthesize high-molecular-weight polypeptide materials. By studying the initiating systems and kinetic models of CCP, it was found that the secondary structure and molecular interactions played a crucial role in the polymerization rate. The fast kinetics of CCP also minimized side reactions and allowed the synthesis of polypeptides in the presence of an aqueous phase.
ACCOUNTS OF MATERIALS RESEARCH
(2023)
Article
Polymer Science
Qinyuan Li, Yingjia Lan, Wanying Wang, Guonan Ji, Xiaohong Li, Ziyuan Song
Summary: This study reports a method to control the polymerization behavior using a cosolvent system, which alters the polymerization kinetics curve and increases monomer solubility by mixing with chloroform, enabling fast and controlled preparation of polypeptides.
Review
Chemistry, Multidisciplinary
Zhiyuan Shi, Yong Hu, Xin Li
Summary: Controlled drug delivery systems that can respond to mechanical force offer a unique solution for on-demand drug activation and release. Among various mechanical stimuli, ultrasound (US) has advantages in achieving spatiotemporally controlled drug release. Traditional US-triggered drug release relies on heat-induced phase transitions or chemical transformations, while the cutting-edge approach of Sonopharmacology leverages polymer mechanochemistry. The remaining challenges and potential future directions in this field are also discussed.
JOURNAL OF CONTROLLED RELEASE
(2024)
Article
Chemistry, Multidisciplinary
Lijie Zheng, Yuanzheng Chen, Xun Gu, Yingying Li, Hanqing Zhao, Wenjun Shao, Tao Ma, Chuanbin Wu, Qingqing Wang
Summary: In this study, a novel dosage form consisting of dissolving microneedles and an adhesive transdermal patch was developed for the treatment of rheumatoid arthritis. In vitro and in vivo experiments demonstrated that the combination of drugs delivered by this dosage form effectively reduced joint inflammation and damage.
JOURNAL OF CONTROLLED RELEASE
(2024)
Article
Chemistry, Multidisciplinary
Kyungjik Yang, Hwa Seung Han, Seung Hwan An, Kyung Hoon Park, Keonwook Nam, Shinha Hwang, Yuyeon Lee, Sung Yeon Cho, Taehyung Kim, Deokyeong Choe, Sang Won Kim, Wonkyu Yu, Hyunah Lee, Jiyong Park, Sangguan You, Dong- Gyu Jo, Ki Young Choi, Young Hoon Roh, Jae Hyung Park
Summary: This study developed CP-loaded CS microcapsules to enhance the oral bioavailability of CP through controlled gastrointestinal delivery. The optimized microcapsules exhibited desirable physicochemical properties, showed anti-photoaging effects via antioxidant activity, and achieved controlled release in the gastrointestinal tract. This research provides a simple and economical approach for enhancing the oral bioavailability of CP for customized bioactive compound administration.
JOURNAL OF CONTROLLED RELEASE
(2024)
Article
Chemistry, Multidisciplinary
Huiyang Li, Shuo Liu, Wenjin Dai, Bingmei Yao, Yong Zhou, Sujia Si, Hairong Yu, Riguang Zhao, Fang Jin, Liqun Jiang
Summary: Changes in bodily fluid pressures are crucial in diseases like high-altitude pulmonary edema (HAPE). Researchers have developed hydrostatic pressure-sensitive multivesicular liposomes (PSMVLs) that can release drugs in response to pressure changes, with potential applications in HAPE treatment. Animal experiments showed that this system provides better protection for lung tissues and respiratory function, reducing the occurrence of pulmonary edema.
JOURNAL OF CONTROLLED RELEASE
(2024)
Article
Chemistry, Multidisciplinary
Qian Hu, Hongbing Lan, Yinmei Tian, Xiaonan Li, Mengmeng Wang, Jiao Zhang, Yulin Yu, Wei Chen, Li Kong, Yuanyuan Guo, Zhiping Zhang
Summary: Coacervate droplets formed through liquid-liquid phase separation have potential as delivery vesicles for therapeutics. However, their lack of physiological stability and membranes are challenges. In this study, polylysine-polynucleotide complex coacervate droplets with favorable stability were formulated to concentrate molecules and nanoparticles. Phospholipid membranes were further coated on the droplets to create coacervate-based artificial protocells (ArtPC) with membrane-like structures. These biofunctional ArtPC effectively reduced blood uric acid levels and prevented renal injuries.
JOURNAL OF CONTROLLED RELEASE
(2024)
Article
Chemistry, Multidisciplinary
Xiaowei Wang, Hongwei Lu, Fang Luo, Dan Wang, Apeng Wang, Xuelei Wang, Wenkai Feng, Xiaobo Wang, Jiayi Su, Mingliang Liu, Guimin Xia
Summary: Four novel lipid-like GEM diesters were synthesized and encapsulated into liposomes to improve the antitumor efficacy of Gemcitabine. The liposomes loaded with dimyristoyl GEM (LipodmGEM) showed enhanced cellular uptake, improved inhibition of cell migration, and a greatly extended half-life compared to free Gemcitabine. LipodmGEM successfully enriched the drug in the tumor and exhibited excellent anticancer efficacy in vivo with negligible systemic toxicity.
JOURNAL OF CONTROLLED RELEASE
(2024)
Article
Chemistry, Multidisciplinary
Pengyu Li, Jieyi Pan, Yating Dong, Yingying Sun, Yalong Wang, Kang Liao, Yili Chen, Xin Deng, Shihui Yu, Haiyan Hu
Summary: Chronic pulmonary infection caused by Pseudomonas aeruginosa is a serious public health problem with high mortality rates. In this study, infection-microenvironment responsive nanoparticles were developed to eradicate biofilms and inhibit virulence. These nanoparticles showed promising results in treating chronic pulmonary infections.
JOURNAL OF CONTROLLED RELEASE
(2024)
Article
Chemistry, Multidisciplinary
Yajing Sun, Ze Lu, John A. Taylor, Jessie L. S. Au
Summary: A recent development in cancer chemotherapy is the use of cytotoxics to induce tumor-specific immune response through immunogenic cell death (ICD). This study describes a method that utilizes immunostaining and machine-learning to identify cells with ecto-CRT in intact 3-dimensional tissues. The method was successfully applied to study drug-induced ICD in human bladder cancer.
JOURNAL OF CONTROLLED RELEASE
(2024)
Review
Chemistry, Multidisciplinary
Rafat Ali, Shantanu Sen, Rohil Hameed, Aamir Nazir, Sandeep Verma
Summary: This review provides a focused overview of emerging strategies for delivering gasotransmitters in a controlled and sustained manner to reestablish neurophysiological homeostasis.
JOURNAL OF CONTROLLED RELEASE
(2024)
Article
Chemistry, Multidisciplinary
Jing Chen, Xinyi Zhang, Jinshen Zhang, Zhaoxia Wang, Guilan Zhu, Ming Geng, Jinmiao Zhu, Yajun Chen, Wei Wang, Youcui Xu
Summary: In this study, a multifunctional responsive hydrogel system was developed for synergistic reoxygenation and chemo/photothermal therapy. The hydrogel system showed both therapeutic effects against metastatic breast cancer and wound infection, making it a promising strategy for treating and preventing tumor recurrence and associated wound infection.
JOURNAL OF CONTROLLED RELEASE
(2024)
Article
Chemistry, Multidisciplinary
Baoshan Huang, Na Zhang, Xinying Qiu, Rui Zeng, Shuimiao Wang, Mengxia Hua, Qing Li, Kaihui Nan, Sen Lin
Summary: This study revealed that robust ROS can oxidize mitochondrial DNA (ox-mtDNA) and cause its release into the cytosol, resulting in the activation of NLRP3 inflammasome. By using the mitochondria-targeted antioxidant SkQ1 and a novel mitochondria-targeted nanoparticle (SkQ1 NP), it was found that mitochondrial ROS scavenging could in situ inhibit DED-induced mtDNA oxidation and suppress NLRP3-mediated inflammation.
JOURNAL OF CONTROLLED RELEASE
(2024)
Article
Chemistry, Multidisciplinary
Wenqi Liu, Cheng Hu, Linyu Long, Shuyi He, Wen Zhang, Zhicun Wang, Li Yang, Yunbing Wang
Summary: Myocardial infarction is the leading cause of cardiovascular mortality, and current treatment methods have limitations. This study developed a smart carrier that can release different therapeutic substances for different pathological processes, effectively improving cardiac function, promoting cardiac repair, and preventing ventricular remodeling.
JOURNAL OF CONTROLLED RELEASE
(2024)