期刊
BRITISH JOURNAL OF NUTRITION
卷 112, 期 4, 页码 486-492出版社
CAMBRIDGE UNIV PRESS
DOI: 10.1017/S000711451400107X
关键词
Short bowel syndrome; Intestinal barrier; Bovine lactoferrin
资金
- National Nature Science Foundation of China [81200652]
- Shanghai Key Laboratory [11DZ2260500]
- Mead Johnson Nutrition
Previous studies have shown that bovine lactoferrin (bLF) exerts antibacterial, immune-modulating and anti-inflammatory effects. The present study aimed to investigate the effect of enteral bLF supplementation on intestinal adaptation and barrier function in a rat model of short bowel syndrome (SBS). Male Sprague-Dawley rats aged 4 weeks were randomised into three groups (n 10 per group): Sham group (rats submitted to bowel transection and reanastomosis); SBS group (rats submitted to 80% small-bowel resection); SBS-bLF group (rats submitted to 80% small-bowel resection plus treatment with bLF (0.5 g/kg per d) by oral administration from day 2 to day 20). Despite similar food intake, both the SBS and SBS-bLF groups exhibited significantly lower body weight gain, but increased villus height and crypt depth and a higher intestinal epithelial cell proliferation index (P<0.05) when compared with the Sham group. Compared with that in the SBS group, in the SBS-bLF group, bacterial translocation to regional organs was low and intestinal permeability was significantly reduced. The SBS-bLF group also had increased secretory IgA (sIgA) concentrations in ileal contents (29.9 (23.8-33.0) ng/ml), when compared with the other two groups having similar sIgA concentrations (17.5 (12.6-29.1) and 19.3 (11.5-27.0) ng/ml, respectively). The relative expression levels of two tight junction (TJ) proteins, occludin and claudin-4, in the SBS-bLF group were significantly higher than those in the SBS group (P<0.05), but did not exhibit any significant differences when compared with those in the Sham group. In conclusion, enteral bLF supplementation up-regulates small-bowel sIgA concentrations and TJ protein expression and reduces intestinal permeability and could thus support intestinal barrier integrity and protect against bacterial infections in SBS.
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