期刊
BRITISH JOURNAL OF NUTRITION
卷 110, 期 8, 页码 1421-1428出版社
CAMBRIDGE UNIV PRESS
DOI: 10.1017/S0007114513000755
关键词
High-fat feeding; Clock genes; Resveratrol; Rats
资金
- Instituto de Salud Carlos III (RETIC PREDIMED, CIBERObn)
- Spanish Government of Science and Innovation [BFU2011-24720, AGL2011-27406-ALI]
- Tomas Pascual Foundation
- Pilar Gomez-Cuetara Foundation
- Government of the Basque Country [IT-386-10, IT-572-13]
- Seneca Foundation from the Government of Murcia [15123/PI/10]
- University of the Basque Country (UPV/EHU) [ELDUNANOTEK UFI11/32]
In mammals, the main component of the circadian system is the suprachiasmatic nucleus in the hypothalamus. However, circadian clocks are also present in most peripheral tissues, such as adipose tissue. The aim of the present study was to analyse the potential effects of resveratrol on changes induced by high-fat feeding in the expression of clock genes and clock-controlled genes in the white adipose tissue from rats. For this purpose, rats were divided into three groups: a control group, fed a standard diet, and two other groups, either fed a high-fat diet supplemented with resveratrol (RSV) or no resveratrol (HF). The expression of clock genes and clock-controlled genes was analysed by RT-PCR. Protein expression and fatty acid synthase (FAS) activity were also analysed. When comparing the controls, the RSV group showed similar patterns of response to the HF group, except for reverse erythroblastosis virus alpha (Rev-Erb alpha), which was down-regulated. The expression of this gene reached the same levels as in control rats. The response pattern of protein expression for Rev-Erb alpha was similar to that found for gene expression. High-fat feeding up-regulated all adipogenic genes and resveratrol did not modify them. In the HF group, the activity of FAS tended to increase, while resveratrol decreased. In conclusion, resveratrol reverses the change induced by high-fat feeding in the expression of Rev-Erb alpha in adipose tissue, which means that clock machinery is a target for this polyphenol. This change seems to be related to reduced lipogenesis, which might be involved in the body fat-lowering effect of this molecule.
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